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The map label for this gene is pleD [H]

Identifier: 209694808

GI number: 209694808

Start: 1379624

End: 1380955

Strand: Direct

Name: pleD [H]

Synonym: VSAL_I1271

Alternate gene names: 209694808

Gene position: 1379624-1380955 (Clockwise)

Preceding gene: 209694805

Following gene: 209694812

Centisome position: 41.49

GC content: 38.44

Gene sequence:

>1332_bases
ATGCGGGTATTTCAAAAAATAATAATAGGAATGCTAATCCTCTCAATTAGTGCACTTATTTGGCATCATTTTTGGGGGGA
TACTAAAACATTAACAATTAGTCCTGAAAAATACAGCTATTACGGCACTGATGATCGCAAGGTTGGAGGGATTAGTGAAG
CAACATTAATCGATAAAAATAATGTGCCAAGCATGTTGTGTAATATGAAAAAAGGGGATTATGCTTGGCCTTATTGCGAG
GCTGCGATTCATTTATCAGGCAGTATTTTTGAAGGTATCGATCTCTCGGATTATTCTACCCTGGTACTCGATATCGATTA
TAAAAGTACCGATCCTAATGGTCGTTTACGGGTGTATTTACGAAACAGCAACCCGGCATACACTAAACGCAACGACGCGG
CGTCATTAAAATTTAATGGCATCGAATATGAACCTGGATTTAAAACGGGTCCAAAAGAAATATTATTGTCGGATTTTCAA
GTATTAACGTGGTGGATTGCGGATTACAAAGTAGCCGTAGAACATGCTGCGCCTGAATTTTCTAATGTATCGATTATTGA
AATTGCGACGTCTTCAGGCCTTAGTGTCGGTAATTTTGAAATGACAATTAATAGTATTGAACTGCGTGGTTCTTGGGTTA
AAGAAACGACCTTATTGAAATTTATTTTGTATGCTTGGTTGGTCATGATCATTGGTTACATCCTTTATGAACAAAAAGGG
TTAAATCGACGTCTTAAAGCCAGTACGTATCGTGAAACCAAATTACAAAAGATGAATGATAATTTAAAAGAAAAAAATGT
TGAGTTTGCCGAGTTAGCACACCGAGATTCGTTAACCGGTGCGAGAAATCGAAACGCAGTAAGGGATTGGTTAGACAGAA
TGTCTCAACAAGTACGTTGGGGTAAACAACAATTCTCTGTTATCTACATTGATATTGATTTTTTTAAAGGTATTAATGAC
AAGTTAGGTCACCAAGTTGGGGATGATATTTTACGAGAATTTGTTTTGGTGGTGACGGGATCGATTAAAACGACTGATTT
CCTCGTTCGTTGGGGAGGTGAAGAGTTTATTGTTTTTAGTCCACAGACTGGTCTGGATCAAGCGTTTGAAAAAGCAGAGC
ATATTCGACGTACTGTTGAGCATCACCTTTGGTGTCATAATGAATCTTTGACGTGTAGCTTTGGTGTGGCAGAAATGGGG
AATGAGCGTATTTCCGAAGTGATAGCAAGGGCGGATGACGCGCTATATAAAGCTAAAAATCAAGGGCGTAATAGGGTGGT
GAGAAGTAATAATCTGCCCGATCAAAGATTGGTTTCTCGTTTTATGGATTAG

Upstream 100 bases:

>100_bases
AGTCAATAACAAAGTATTATTTCTTTGCGATGAAAATTAAGTGCGGTATTATTTTTTTGGAATGGAGATCAACTTTTACT
GGCTATGAAAGGAACGTCGT

Downstream 100 bases:

>100_bases
TCTTCTGCTATAAAAAGCCTCGCTATTGTATATACATAGCGAGGCTTTTTTTATTTAATCGAATTATTCAACAGGAATAG
TTTTATGCTGGATAGGTTCT

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MRVFQKIIIGMLILSISALIWHHFWGDTKTLTISPEKYSYYGTDDRKVGGISEATLIDKNNVPSMLCNMKKGDYAWPYCE
AAIHLSGSIFEGIDLSDYSTLVLDIDYKSTDPNGRLRVYLRNSNPAYTKRNDAASLKFNGIEYEPGFKTGPKEILLSDFQ
VLTWWIADYKVAVEHAAPEFSNVSIIEIATSSGLSVGNFEMTINSIELRGSWVKETTLLKFILYAWLVMIIGYILYEQKG
LNRRLKASTYRETKLQKMNDNLKEKNVEFAELAHRDSLTGARNRNAVRDWLDRMSQQVRWGKQQFSVIYIDIDFFKGIND
KLGHQVGDDILREFVLVVTGSIKTTDFLVRWGGEEFIVFSPQTGLDQAFEKAEHIRRTVEHHLWCHNESLTCSFGVAEMG
NERISEVIARADDALYKAKNQGRNRVVRSNNLPDQRLVSRFMD

Sequences:

>Translated_443_residues
MRVFQKIIIGMLILSISALIWHHFWGDTKTLTISPEKYSYYGTDDRKVGGISEATLIDKNNVPSMLCNMKKGDYAWPYCE
AAIHLSGSIFEGIDLSDYSTLVLDIDYKSTDPNGRLRVYLRNSNPAYTKRNDAASLKFNGIEYEPGFKTGPKEILLSDFQ
VLTWWIADYKVAVEHAAPEFSNVSIIEIATSSGLSVGNFEMTINSIELRGSWVKETTLLKFILYAWLVMIIGYILYEQKG
LNRRLKASTYRETKLQKMNDNLKEKNVEFAELAHRDSLTGARNRNAVRDWLDRMSQQVRWGKQQFSVIYIDIDFFKGIND
KLGHQVGDDILREFVLVVTGSIKTTDFLVRWGGEEFIVFSPQTGLDQAFEKAEHIRRTVEHHLWCHNESLTCSFGVAEMG
NERISEVIARADDALYKAKNQGRNRVVRSNNLPDQRLVSRFMD
>Mature_443_residues
MRVFQKIIIGMLILSISALIWHHFWGDTKTLTISPEKYSYYGTDDRKVGGISEATLIDKNNVPSMLCNMKKGDYAWPYCE
AAIHLSGSIFEGIDLSDYSTLVLDIDYKSTDPNGRLRVYLRNSNPAYTKRNDAASLKFNGIEYEPGFKTGPKEILLSDFQ
VLTWWIADYKVAVEHAAPEFSNVSIIEIATSSGLSVGNFEMTINSIELRGSWVKETTLLKFILYAWLVMIIGYILYEQKG
LNRRLKASTYRETKLQKMNDNLKEKNVEFAELAHRDSLTGARNRNAVRDWLDRMSQQVRWGKQQFSVIYIDIDFFKGIND
KLGHQVGDDILREFVLVVTGSIKTTDFLVRWGGEEFIVFSPQTGLDQAFEKAEHIRRTVEHHLWCHNESLTCSFGVAEMG
NERISEVIARADDALYKAKNQGRNRVVRSNNLPDQRLVSRFMD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=41.2121212121212, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=36.8098159509202, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=37.4233128834356, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=32.7044025157233, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=35.8974358974359, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787541, Length=159, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=32.3529411764706, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=31.8471337579618, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=36.094674556213, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=31.3609467455621, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=27.8145695364238, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50776; Mature: 50776

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFQKIIIGMLILSISALIWHHFWGDTKTLTISPEKYSYYGTDDRKVGGISEATLIDKN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEECCC
NVPSMLCNMKKGDYAWPYCEAAIHLSGSIFEGIDLSDYSTLVLDIDYKSTDPNGRLRVYL
CCCHHHHCCCCCCCCCCHHHHHEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEE
RNSNPAYTKRNDAASLKFNGIEYEPGFKTGPKEILLSDFQVLTWWIADYKVAVEHAAPEF
ECCCCCCCCCCCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
SNVSIIEIATSSGLSVGNFEMTINSIELRGSWVKETTLLKFILYAWLVMIIGYILYEQKG
CCEEEEEEECCCCCEECEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LNRRLKASTYRETKLQKMNDNLKEKNVEFAELAHRDSLTGARNRNAVRDWLDRMSQQVRW
CCCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
GKQQFSVIYIDIDFFKGINDKLGHQVGDDILREFVLVVTGSIKTTDFLVRWGGEEFIVFS
CCCCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEC
PQTGLDQAFEKAEHIRRTVEHHLWCHNESLTCSFGVAEMGNERISEVIARADDALYKAKN
CCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEECCHHHHCHHHHHHHHHHHHHHHHHHHH
QGRNRVVRSNNLPDQRLVSRFMD
CCCCCEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MRVFQKIIIGMLILSISALIWHHFWGDTKTLTISPEKYSYYGTDDRKVGGISEATLIDKN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEECCC
NVPSMLCNMKKGDYAWPYCEAAIHLSGSIFEGIDLSDYSTLVLDIDYKSTDPNGRLRVYL
CCCHHHHCCCCCCCCCCHHHHHEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEE
RNSNPAYTKRNDAASLKFNGIEYEPGFKTGPKEILLSDFQVLTWWIADYKVAVEHAAPEF
ECCCCCCCCCCCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
SNVSIIEIATSSGLSVGNFEMTINSIELRGSWVKETTLLKFILYAWLVMIIGYILYEQKG
CCEEEEEEECCCCCEECEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LNRRLKASTYRETKLQKMNDNLKEKNVEFAELAHRDSLTGARNRNAVRDWLDRMSQQVRW
CCCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
GKQQFSVIYIDIDFFKGINDKLGHQVGDDILREFVLVVTGSIKTTDFLVRWGGEEFIVFS
CCCCEEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEC
PQTGLDQAFEKAEHIRRTVEHHLWCHNESLTCSFGVAEMGNERISEVIARADDALYKAKN
CCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEECCHHHHCHHHHHHHHHHHHHHHHHHHH
QGRNRVVRSNNLPDQRLVSRFMD
CCCCCEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA