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The map label for this gene is menE [H]

Identifier: 209694661

GI number: 209694661

Start: 1199768

End: 1201165

Strand: Direct

Name: menE [H]

Synonym: VSAL_I1103

Alternate gene names: 209694661

Gene position: 1199768-1201165 (Clockwise)

Preceding gene: 209694660

Following gene: 209694662

Centisome position: 36.08

GC content: 42.2

Gene sequence:

>1398_bases
ATGGGAAAGTTAATTACGCCTGAATTTACAACTTGGCCGTGGGTTAAGTGGAGTGAAACACGTGAGCTTGAAATCGCATT
ACGAGATGGTGATACGATCTGGACATGGGCTGAAGTATCGACTCAAATAAATCGCTACGCGAACGGATTACTTCAACAAG
GGGTTAAACGTGACGAAGTCGTTGTCGGTATTTCTAAGAACTGCATTGAATTAGTCTGGTTGCAACTTGCTTGTGTCCGT
ATCGGAGCACGTTTTGCAAGTATTAATCCAAAAAAACCAGAGTGCGAACTTCATCAGTTAGTCACGAGTATTTCTGCGAG
TCATATTTGGTTTGGAAAAGACCAAAAAACAATCTCGGGAATTTGGCACCATTTACAATTGATTAATGCTGAATATGGCG
TCATAGCGGCCACTTGGCAGTTGGGTCGGTTAGCCACATTGACCTTTACTTCTGGATCGACGGGCTTACCAAAAGCCGTT
GCACACAGTGCGAATAACCATTTACATTCTGCGGCAGGTTTACTTAGTTGGATGGATTTTGATGTATGCGATTCGTGGTT
ATTATCGTTACCACTATTTCATGTATCTGGTCTTGCGATTGTGTGGCGCTGGTTACTCTCAGGTGCAACATTAGTCATGC
CGACATCGAACGGACTTCTTCATGATTTATCTAGTGTCACTCATGCGTCATTAGTGCCAACACAGCTAACTCAAATCTTG
GCGGACGACCAAGTATCCCGTTTAGCCTTAAAGCGCGTATTGTTAGGGGGAGCCGTTATTCCTCCAGTATTAACAGAGGC
GGCTGAATTGCAAGGTATTGAATGCTGGTTAGGCTATGGTATGACAGAGATGGGTTCTACTGTTACCGCTAAGCGAGCGG
ATGGTCATACTGGCGTTGGAAATGTCCTCCCTTATCGAGAGATTAAAATTGATCATGGTGAGGTTTTAGTTCGAGGCGAA
ACGTTAGCACTTGGCTATTATCGTTCATCTGGGATCATCGATATTGCTGAAGACGATGGTTGGTTTAAAACAAAAGATTT
AGGTGATGTTTCTACGGGTGAGCTGCATATTAAAGGACGTGCTGATAACATGTTTATTTCTGGAGGGGAGAATATACACC
CTGAAAAAATTGAACAAGCGTTATTATTGCATCCAATGATTGAGCAAGTGATTGTCGTTGAAAGCGTGGATGAACAATTT
GGTTATCGACCTGTTGCCGCTGTTGTTACTACATCAGAGATTAAGGAAGAAGAGCTTACTTATTTCTTATCTGACAAACT
TACCTCGTATGAATTGCCTATTCGTTATGAATTAATGCCTAAGTCGTTACTCAATACGGGCATTAAAATATCTCGACAAA
AAGTAAGAGAGTGGGTGAATGATTTATTACCCGCATAA

Upstream 100 bases:

>100_bases
TCCCTGGTTTAGATACCATCGATTTATTTAAAATGCAGTTAGACACACCGTGGCCTCATTGTGAGTTACCGATGCAAGCG
CTGTCCGATCTCGAGGTGAT

Downstream 100 bases:

>100_bases
ATAAATTATCAGGGTGTGCATCTTATTAAAGATATCGTGCTTTAATAAATCGAGATACACGCCCTGAAAATCGGTAAAAA
AGATACACTGTTACGCATAA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGKLITPEFTTWPWVKWSETRELEIALRDGDTIWTWAEVSTQINRYANGLLQQGVKRDEVVVGISKNCIELVWLQLACVR
IGARFASINPKKPECELHQLVTSISASHIWFGKDQKTISGIWHHLQLINAEYGVIAATWQLGRLATLTFTSGSTGLPKAV
AHSANNHLHSAAGLLSWMDFDVCDSWLLSLPLFHVSGLAIVWRWLLSGATLVMPTSNGLLHDLSSVTHASLVPTQLTQIL
ADDQVSRLALKRVLLGGAVIPPVLTEAAELQGIECWLGYGMTEMGSTVTAKRADGHTGVGNVLPYREIKIDHGEVLVRGE
TLALGYYRSSGIIDIAEDDGWFKTKDLGDVSTGELHIKGRADNMFISGGENIHPEKIEQALLLHPMIEQVIVVESVDEQF
GYRPVAAVVTTSEIKEEELTYFLSDKLTSYELPIRYELMPKSLLNTGIKISRQKVREWVNDLLPA

Sequences:

>Translated_465_residues
MGKLITPEFTTWPWVKWSETRELEIALRDGDTIWTWAEVSTQINRYANGLLQQGVKRDEVVVGISKNCIELVWLQLACVR
IGARFASINPKKPECELHQLVTSISASHIWFGKDQKTISGIWHHLQLINAEYGVIAATWQLGRLATLTFTSGSTGLPKAV
AHSANNHLHSAAGLLSWMDFDVCDSWLLSLPLFHVSGLAIVWRWLLSGATLVMPTSNGLLHDLSSVTHASLVPTQLTQIL
ADDQVSRLALKRVLLGGAVIPPVLTEAAELQGIECWLGYGMTEMGSTVTAKRADGHTGVGNVLPYREIKIDHGEVLVRGE
TLALGYYRSSGIIDIAEDDGWFKTKDLGDVSTGELHIKGRADNMFISGGENIHPEKIEQALLLHPMIEQVIVVESVDEQF
GYRPVAAVVTTSEIKEEELTYFLSDKLTSYELPIRYELMPKSLLNTGIKISRQKVREWVNDLLPA
>Mature_464_residues
GKLITPEFTTWPWVKWSETRELEIALRDGDTIWTWAEVSTQINRYANGLLQQGVKRDEVVVGISKNCIELVWLQLACVRI
GARFASINPKKPECELHQLVTSISASHIWFGKDQKTISGIWHHLQLINAEYGVIAATWQLGRLATLTFTSGSTGLPKAVA
HSANNHLHSAAGLLSWMDFDVCDSWLLSLPLFHVSGLAIVWRWLLSGATLVMPTSNGLLHDLSSVTHASLVPTQLTQILA
DDQVSRLALKRVLLGGAVIPPVLTEAAELQGIECWLGYGMTEMGSTVTAKRADGHTGVGNVLPYREIKIDHGEVLVRGET
LALGYYRSSGIIDIAEDDGWFKTKDLGDVSTGELHIKGRADNMFISGGENIHPEKIEQALLLHPMIEQVIVVESVDEQFG
YRPVAAVVTTSEIKEEELTYFLSDKLTSYELPIRYELMPKSLLNTGIKISRQKVREWVNDLLPA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=25.3333333333333, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI122937307, Length=359, Percent_Identity=27.5766016713092, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788595, Length=453, Percent_Identity=43.2671081677704, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI145693145, Length=489, Percent_Identity=23.721881390593, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786810, Length=499, Percent_Identity=23.6472945891784, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788107, Length=347, Percent_Identity=26.5129682997118, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI221142682, Length=489, Percent_Identity=23.3128834355828, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=481, Percent_Identity=27.6507276507277, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=465, Percent_Identity=25.5913978494624, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=495, Percent_Identity=26.6666666666667, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=497, Percent_Identity=26.1569416498994, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=410, Percent_Identity=25.1219512195122, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=330, Percent_Identity=29.3939393939394, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994694, Length=532, Percent_Identity=24.0601503759398, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=532, Percent_Identity=24.0601503759398, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994690, Length=532, Percent_Identity=24.0601503759398, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=347, Percent_Identity=25.0720461095101, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24581924, Length=354, Percent_Identity=26.5536723163842, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51548; Mature: 51417

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLITPEFTTWPWVKWSETRELEIALRDGDTIWTWAEVSTQINRYANGLLQQGVKRDEV
CCCCCCCCCCCCCCEEECCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCE
VVGISKNCIELVWLQLACVRIGARFASINPKKPECELHQLVTSISASHIWFGKDQKTISG
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHH
IWHHLQLINAEYGVIAATWQLGRLATLTFTSGSTGLPKAVAHSANNHLHSAAGLLSWMDF
HHHHHHHHCCCCCEEEEEEECCEEEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
DVCDSWLLSLPLFHVSGLAIVWRWLLSGATLVMPTSNGLLHDLSSVTHASLVPTQLTQIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHH
ADDQVSRLALKRVLLGGAVIPPVLTEAAELQGIECWLGYGMTEMGSTVTAKRADGHTGVG
HCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHHCCEEEEECCCCCCCCC
NVLPYREIKIDHGEVLVRGETLALGYYRSSGIIDIAEDDGWFKTKDLGDVSTGELHIKGR
CCCCCEEEEECCCCEEEECCEEEEEEEECCCEEEEECCCCCEEECCCCCCCCCEEEEEEC
ADNMFISGGENIHPEKIEQALLLHPMIEQVIVVESVDEQFGYRPVAAVVTTSEIKEEELT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
YFLSDKLTSYELPIRYELMPKSLLNTGIKISRQKVREWVNDLLPA
HHHHCCCCCCCCCEEEEECCHHHHHCCCEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKLITPEFTTWPWVKWSETRELEIALRDGDTIWTWAEVSTQINRYANGLLQQGVKRDEV
CCCCCCCCCCCCCEEECCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCE
VVGISKNCIELVWLQLACVRIGARFASINPKKPECELHQLVTSISASHIWFGKDQKTISG
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHH
IWHHLQLINAEYGVIAATWQLGRLATLTFTSGSTGLPKAVAHSANNHLHSAAGLLSWMDF
HHHHHHHHCCCCCEEEEEEECCEEEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
DVCDSWLLSLPLFHVSGLAIVWRWLLSGATLVMPTSNGLLHDLSSVTHASLVPTQLTQIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHH
ADDQVSRLALKRVLLGGAVIPPVLTEAAELQGIECWLGYGMTEMGSTVTAKRADGHTGVG
HCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHHCCEEEEECCCCCCCCC
NVLPYREIKIDHGEVLVRGETLALGYYRSSGIIDIAEDDGWFKTKDLGDVSTGELHIKGR
CCCCCEEEEECCCCEEEECCEEEEEEEECCCEEEEECCCCCEEECCCCCCCCCEEEEEEC
ADNMFISGGENIHPEKIEQALLLHPMIEQVIVVESVDEQFGYRPVAAVVTTSEIKEEELT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
YFLSDKLTSYELPIRYELMPKSLLNTGIKISRQKVREWVNDLLPA
HHHHCCCCCCCCCEEEEECCHHHHHCCCEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]