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The map label for this gene is truA

Identifier: 209694634

GI number: 209694634

Start: 1170713

End: 1171501

Strand: Direct

Name: truA

Synonym: VSAL_I1075

Alternate gene names: 209694634

Gene position: 1170713-1171501 (Clockwise)

Preceding gene: 209694633

Following gene: 209694635

Centisome position: 35.21

GC content: 41.06

Gene sequence:

>789_bases
ATGCGAATAGCTTTATGTATTGAGTACGATGGTGCCAGTTACCACGGTTGGCAGTCCCAAAAAGACGTAACAAGTGTTCA
AGAAGAATTAGAAAAAGCCTTAATGGTCGTTGCTAATGAACCGATTGAGGTTATGTGTGCAGGTCGAACGGATGCTGGCG
TACATGGAACAGGTCAAGTAGTACATTTTGATACAAATAGCGTGCGTAAATTGCCAGTGTGGATGGTAGGGGTGAATGCT
AACCTACCAAAAAATATTGCGGTTCGTTGGGTTAAAGAAGTAAACGAAGATTTTCATGCTCGTTTTACTGCTACCGCGCG
ACGTTATCGTTATATTATTTTTAATAATCGTCAACGCCCAGCCATTTTTAGTCATGGTGTTAGTCATTATCATGAAACAC
TTGATGCTGATTTAATGCATGAAGCTGGCCAGTATCTACTGGGAGAGAATGATTTTACGTCTTTTAGAGCGGTTCGCTGT
CAGTCACGTACCCCTTGGCGTAATTTAATACACTTAAAAGTAACGCGTCATGGCGATTTTATTGTTATTGATATTAAAGC
GAATGCCTTTGTTCATCATATGGTCCGAAACATTACAGGTAGCCTAATCGAAGTGGGCAGAGGCAAGCAAAAACCTGAGT
GGGTACAGTGGTTACTAGAAGCGAAAGACCGTAAGCTAGCAGGAGCAACAGCAAAAGCTGAAGGATTATATCTTATTGAT
GTTGATTACCCTAAAGAGTGGGATTTGCCTAGAGTTCCGTTAGGGCCTTTATTTTTACCAGATAATTGA

Upstream 100 bases:

>100_bases
AACTCGATTAACTTTTACATCAACAATCTTAAAAAGGCGGTGGCAGTGAGCTCCGCCTTTTTTTATAATGGCGTATAACT
GAATAAAAAGAATTGAAATC

Downstream 100 bases:

>100_bases
TTCAATTAATGATAAAGATTAAACCTTAAAATAGTGTAAAAAGTAGATGTGGATTCAATATTGAAAATAGGTACAACCAG
TTTTTTATCTACACTGGTCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIALCIEYDGASYHGWQSQKDVTSVQEELEKALMVVANEPIEVMCAGRTDAGVHGTGQVVHFDTNSVRKLPVWMVGVNA
NLPKNIAVRWVKEVNEDFHARFTATARRYRYIIFNNRQRPAIFSHGVSHYHETLDADLMHEAGQYLLGENDFTSFRAVRC
QSRTPWRNLIHLKVTRHGDFIVIDIKANAFVHHMVRNITGSLIEVGRGKQKPEWVQWLLEAKDRKLAGATAKAEGLYLID
VDYPKEWDLPRVPLGPLFLPDN

Sequences:

>Translated_262_residues
MRIALCIEYDGASYHGWQSQKDVTSVQEELEKALMVVANEPIEVMCAGRTDAGVHGTGQVVHFDTNSVRKLPVWMVGVNA
NLPKNIAVRWVKEVNEDFHARFTATARRYRYIIFNNRQRPAIFSHGVSHYHETLDADLMHEAGQYLLGENDFTSFRAVRC
QSRTPWRNLIHLKVTRHGDFIVIDIKANAFVHHMVRNITGSLIEVGRGKQKPEWVQWLLEAKDRKLAGATAKAEGLYLID
VDYPKEWDLPRVPLGPLFLPDN
>Mature_262_residues
MRIALCIEYDGASYHGWQSQKDVTSVQEELEKALMVVANEPIEVMCAGRTDAGVHGTGQVVHFDTNSVRKLPVWMVGVNA
NLPKNIAVRWVKEVNEDFHARFTATARRYRYIIFNNRQRPAIFSHGVSHYHETLDADLMHEAGQYLLGENDFTSFRAVRC
QSRTPWRNLIHLKVTRHGDFIVIDIKANAFVHHMVRNITGSLIEVGRGKQKPEWVQWLLEAKDRKLAGATAKAEGLYLID
VDYPKEWDLPRVPLGPLFLPDN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=70.7692307692308, Blast_Score=394, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321112, Length=279, Percent_Identity=27.5985663082437, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281362146, Length=273, Percent_Identity=25.2747252747253, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648455, Length=236, Percent_Identity=25.8474576271186, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_ALISL (B6EIW6)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262562.1
- GeneID:   6986834
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1075
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29942; Mature: 29942

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALCIEYDGASYHGWQSQKDVTSVQEELEKALMVVANEPIEVMCAGRTDAGVHGTGQV
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCE
VHFDTNSVRKLPVWMVGVNANLPKNIAVRWVKEVNEDFHARFTATARRYRYIIFNNRQRP
EEECCCCCEECEEEEEEECCCCCHHHHHHHHHHHCHHHHHEEEEEEEEEEEEEECCCCCC
AIFSHGVSHYHETLDADLMHEAGQYLLGENDFTSFRAVRCQSRTPWRNLIHLKVTRHGDF
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCHHEEEEEEEECCCE
IVIDIKANAFVHHMVRNITGSLIEVGRGKQKPEWVQWLLEAKDRKLAGATAKAEGLYLID
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VDYPKEWDLPRVPLGPLFLPDN
ECCCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure
MRIALCIEYDGASYHGWQSQKDVTSVQEELEKALMVVANEPIEVMCAGRTDAGVHGTGQV
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCE
VHFDTNSVRKLPVWMVGVNANLPKNIAVRWVKEVNEDFHARFTATARRYRYIIFNNRQRP
EEECCCCCEECEEEEEEECCCCCHHHHHHHHHHHCHHHHHEEEEEEEEEEEEEECCCCCC
AIFSHGVSHYHETLDADLMHEAGQYLLGENDFTSFRAVRCQSRTPWRNLIHLKVTRHGDF
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCHHEEEEEEEECCCE
IVIDIKANAFVHHMVRNITGSLIEVGRGKQKPEWVQWLLEAKDRKLAGATAKAEGLYLID
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VDYPKEWDLPRVPLGPLFLPDN
ECCCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA