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The map label for this gene is rep [H]

Identifier: 209693716

GI number: 209693716

Start: 122717

End: 124732

Strand: Direct

Name: rep [H]

Synonym: VSAL_I0092

Alternate gene names: 209693716

Gene position: 122717-124732 (Clockwise)

Preceding gene: 209693711

Following gene: 209693718

Centisome position: 3.69

GC content: 39.58

Gene sequence:

>2016_bases
ATGAGACTTAACCCACAGCAAGATGAAGCCGTTAAGTTTGTTTCTGGGCCATGTTTAGTATTGGCAGGCGCAGGCTCAGG
TAAAACTCGTGTAATTACGAATAAAATAGCCTATCTAGTACAACAATGCGGGTATAAAGCTCGCAATATTGCTGCGGTTA
CCTTTACTAATAAAGCGGCGAGAGAGATGAAAGAGCGTGTTGGGCAAACGCTGAACAAACAAGAATCACGAGGGTTATTA
GTATCAACATTCCATTCTCTTGGATTAGACATTATTCGTCGTGAATATAAAATATTAGGCCTAAAGGCAGGTTTTTCTCT
TTTTGATGATCAAGACCAATTAGCCTTATTAAAAGAACTGACTGAAAAACAAATTGATGGAGACAAGGATCTACTACGTC
AACTGCTTAGTTGTATTTCTAAGTGGAAGACTGACATGCTTACCCCAGATCAAGTTAAAGGCATTGTTCGCTCAGAGCAG
GAGCAATTATTTGCTTTCTGTTATGAGATGTATTTAACGCAAATGAAAGCGTATAACGCATTAGATTTTGATGATTTAAT
CTCATTGCCGGTGTTATTGTTGAGAAATAATCAGGATGTACGTGAGCGTTGGCAGATGAAACTTCGCTATTTATTAGTGG
ATGAATATCAAGATACCAATACTAGCCAATATGAATTAGTAAAATTGCTTGTTGGTGAAAGAGGTCGATTGACCGTAGTA
GGAGATGATGATCAATCTATTTATTCGTGGCGTGGAGCAAAACCTCAAAACCTTATTTTATTGAGTGAAGACTACCCTAA
TTTGAAAGTCATTAAATTAGAGCAGAATTATCGTTCAACGAGTCGAATTTTACGTGCAGCCAACATACTGATTGCTAATA
ATCCTCATGCATTTGAAAAACGGTTATTTTCTGAAATCCCTGATGGTGAGCAGATAAAGATTCTGACAGCGAAAGATGAT
GCGCACGAAGCCGAACGAGTTGTCGGTGAGATCATTGCTCATCGTTTCCTTAATCGAACCGATTATAAAGATTACGCAGT
ACTTTATCGTGGGAATCATCAGTCTCGACTTATTGAAAAATCACTAATGCAAAACCGCGTGCCTTATAAGATTTCTGGGG
GGACTTCCTTTTTCTCTCGTTCAGAAATCAAAGACATCATGGCTTATCTTCGTCTTCTGACTAACCCTGATGACGATAAT
GCGTTTTTGCGTGTCGTAAACACACCTCGACGTGAAATTGGCCCTGTTACTTTAGAGAAGCTAGGTACGTATGCCAACAT
GCGTGGAAAAAGTTTGTTTGAAGCTTGTTTTGAAATGGGGTTAGAACAAACCTTATCTGGTCGAGGGCTTGAGTCACTGC
GTCGATTTGGTCATTGGGTTGTTTCTATATCAGAAAATGCTGAGCGAGGTAATACCGTAGAGGCGGTGCGATCACTAGTA
AAAGACATTCACTACGAAGATTGGTTGTACGAAACATCACCTAGCGCGAAAGCTGCAGAAATGCGCATGAAAAATGTATC
TGATTTATATTCTTGGATTGTGGCTGATTTAGAAGGTGATAATTATGATAAAGAAGAGAAAACATTAAAAGAAGTCGTAT
TGCGCTTAACACTGCGAGATATGATGGAGCGTGGAGAAGATGAAGATGGTGCTGACCAAGTGCAGTTAATGACATTGCAT
GCATCAAAAGGCTTAGAATTCCCTTATGTGTATTTGATTGGGGCAGAAGAAGGTATTTTACCGCATCAAACCAGTGTTGA
TGAAGACAACGTAGAAGAAGAACGTCGTTTGGCGTATGTGGGTATTACGCGAGCAAAAAAAGAGCTGACTTTTACATTGT
GTAAAGAGCGTCGTCAGTATGGTGAGCTAATTCGTCCCACACCAAGCCGTTTTTTAGAAGAGCTTCCGCAAGATGATTTA
GATTGGGAAATGAAAAAACCTAAGCCGACTCAAGAAGAGAGAATGGAAAAAGGGAAAAGTAATATTGCTCATCTGCGTGC
TATGTTGAAAAAATAG

Upstream 100 bases:

>100_bases
GTAAAGGTCGAGAGTTTTCTTCTATGGTTTACATGGTATTCATACATAGACAGCTTTATTATAGACGCAAATATTCCATT
ATCGATTTTATAGGTGATTC

Downstream 100 bases:

>100_bases
CCTCTTAATTTAAAGAATAAATAAAAATGCCCACATACCTGATTTGGTATTGTGGGCATTTTTGTACCAGTTAAATAAGC
GGTACCTAATCTGCGGTATC

Product: ATP-dependent DNA helicase rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MRLNPQQDEAVKFVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVGQTLNKQESRGLL
VSTFHSLGLDIIRREYKILGLKAGFSLFDDQDQLALLKELTEKQIDGDKDLLRQLLSCISKWKTDMLTPDQVKGIVRSEQ
EQLFAFCYEMYLTQMKAYNALDFDDLISLPVLLLRNNQDVRERWQMKLRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLILLSEDYPNLKVIKLEQNYRSTSRILRAANILIANNPHAFEKRLFSEIPDGEQIKILTAKDD
AHEAERVVGEIIAHRFLNRTDYKDYAVLYRGNHQSRLIEKSLMQNRVPYKISGGTSFFSRSEIKDIMAYLRLLTNPDDDN
AFLRVVNTPRREIGPVTLEKLGTYANMRGKSLFEACFEMGLEQTLSGRGLESLRRFGHWVVSISENAERGNTVEAVRSLV
KDIHYEDWLYETSPSAKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLKEVVLRLTLRDMMERGEDEDGADQVQLMTLH
ASKGLEFPYVYLIGAEEGILPHQTSVDEDNVEEERRLAYVGITRAKKELTFTLCKERRQYGELIRPTPSRFLEELPQDDL
DWEMKKPKPTQEERMEKGKSNIAHLRAMLKK

Sequences:

>Translated_671_residues
MRLNPQQDEAVKFVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVGQTLNKQESRGLL
VSTFHSLGLDIIRREYKILGLKAGFSLFDDQDQLALLKELTEKQIDGDKDLLRQLLSCISKWKTDMLTPDQVKGIVRSEQ
EQLFAFCYEMYLTQMKAYNALDFDDLISLPVLLLRNNQDVRERWQMKLRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLILLSEDYPNLKVIKLEQNYRSTSRILRAANILIANNPHAFEKRLFSEIPDGEQIKILTAKDD
AHEAERVVGEIIAHRFLNRTDYKDYAVLYRGNHQSRLIEKSLMQNRVPYKISGGTSFFSRSEIKDIMAYLRLLTNPDDDN
AFLRVVNTPRREIGPVTLEKLGTYANMRGKSLFEACFEMGLEQTLSGRGLESLRRFGHWVVSISENAERGNTVEAVRSLV
KDIHYEDWLYETSPSAKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLKEVVLRLTLRDMMERGEDEDGADQVQLMTLH
ASKGLEFPYVYLIGAEEGILPHQTSVDEDNVEEERRLAYVGITRAKKELTFTLCKERRQYGELIRPTPSRFLEELPQDDL
DWEMKKPKPTQEERMEKGKSNIAHLRAMLKK
>Mature_671_residues
MRLNPQQDEAVKFVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVGQTLNKQESRGLL
VSTFHSLGLDIIRREYKILGLKAGFSLFDDQDQLALLKELTEKQIDGDKDLLRQLLSCISKWKTDMLTPDQVKGIVRSEQ
EQLFAFCYEMYLTQMKAYNALDFDDLISLPVLLLRNNQDVRERWQMKLRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLILLSEDYPNLKVIKLEQNYRSTSRILRAANILIANNPHAFEKRLFSEIPDGEQIKILTAKDD
AHEAERVVGEIIAHRFLNRTDYKDYAVLYRGNHQSRLIEKSLMQNRVPYKISGGTSFFSRSEIKDIMAYLRLLTNPDDDN
AFLRVVNTPRREIGPVTLEKLGTYANMRGKSLFEACFEMGLEQTLSGRGLESLRRFGHWVVSISENAERGNTVEAVRSLV
KDIHYEDWLYETSPSAKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLKEVVLRLTLRDMMERGEDEDGADQVQLMTLH
ASKGLEFPYVYLIGAEEGILPHQTSVDEDNVEEERRLAYVGITRAKKELTFTLCKERRQYGELIRPTPSRFLEELPQDDL
DWEMKKPKPTQEERMEKGKSNIAHLRAMLKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=669, Percent_Identity=71.3004484304933, Blast_Score=993, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=661, Percent_Identity=39.4856278366112, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1787196, Length=346, Percent_Identity=28.0346820809249, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=476, Percent_Identity=31.7226890756302, Blast_Score=203, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6324477, Length=679, Percent_Identity=21.2076583210604, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77274; Mature: 77274

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNPQQDEAVKFVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAA
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHCEEEEEECCHHH
REMKERVGQTLNKQESRGLLVSTFHSLGLDIIRREYKILGLKAGFSLFDDQDQLALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHH
TEKQIDGDKDLLRQLLSCISKWKTDMLTPDQVKGIVRSEQEQLFAFCYEMYLTQMKAYNA
HHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDFDDLISLPVLLLRNNQDVRERWQMKLRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
CCHHHHHHHHHHEECCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLILLSEDYPNLKVIKLEQNYRSTSRILRAANILIANNPHAFEK
ECCCCHHHHCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHEEEECCCHHHHH
RLFSEIPDGEQIKILTAKDDAHEAERVVGEIIAHRFLNRTDYKDYAVLYRGNHQSRLIEK
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
SLMQNRVPYKISGGTSFFSRSEIKDIMAYLRLLTNPDDDNAFLRVVNTPRREIGPVTLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCHHHHH
LGTYANMRGKSLFEACFEMGLEQTLSGRGLESLRRFGHWVVSISENAERGNTVEAVRSLV
HHHHHCCCHHHHHHHHHHHCHHHHHCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHH
KDIHYEDWLYETSPSAKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLKEVVLRLTLRD
HHCCHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MMERGEDEDGADQVQLMTLHASKGLEFPYVYLIGAEEGILPHQTSVDEDNVEEERRLAYV
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHEEHE
GITRAKKELTFTLCKERRQYGELIRPTPSRFLEELPQDDLDWEMKKPKPTQEERMEKGKS
EHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
NIAHLRAMLKK
HHHHHHHHHCC
>Mature Secondary Structure
MRLNPQQDEAVKFVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAA
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHCEEEEEECCHHH
REMKERVGQTLNKQESRGLLVSTFHSLGLDIIRREYKILGLKAGFSLFDDQDQLALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHH
TEKQIDGDKDLLRQLLSCISKWKTDMLTPDQVKGIVRSEQEQLFAFCYEMYLTQMKAYNA
HHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDFDDLISLPVLLLRNNQDVRERWQMKLRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
CCHHHHHHHHHHEECCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLILLSEDYPNLKVIKLEQNYRSTSRILRAANILIANNPHAFEK
ECCCCHHHHCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHEEEECCCHHHHH
RLFSEIPDGEQIKILTAKDDAHEAERVVGEIIAHRFLNRTDYKDYAVLYRGNHQSRLIEK
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
SLMQNRVPYKISGGTSFFSRSEIKDIMAYLRLLTNPDDDNAFLRVVNTPRREIGPVTLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCHHHHH
LGTYANMRGKSLFEACFEMGLEQTLSGRGLESLRRFGHWVVSISENAERGNTVEAVRSLV
HHHHHCCCHHHHHHHHHHHCHHHHHCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHH
KDIHYEDWLYETSPSAKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLKEVVLRLTLRD
HHCCHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MMERGEDEDGADQVQLMTLHASKGLEFPYVYLIGAEEGILPHQTSVDEDNVEEERRLAYV
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHEEHE
GITRAKKELTFTLCKERRQYGELIRPTPSRFLEELPQDDLDWEMKKPKPTQEERMEKGKS
EHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
NIAHLRAMLKK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]