Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 209693636

GI number: 209693636

Start: 811

End: 2178

Strand: Reverse

Name: trmE [H]

Synonym: VSAL_I0002

Alternate gene names: 209693636

Gene position: 2178-811 (Counterclockwise)

Preceding gene: 209693637

Following gene: 209693635

Centisome position: 0.07

GC content: 42.76

Gene sequence:

>1368_bases
ATGACATTACATACAGACACCATCGTTGCACAAGCAACAGCCCCTGGACGAGGCGGCGTAGGCATCATTCGAGTATCAGG
CCCTAAAGCTCAACAAGTCGCCCTAGAAGTCGTAGGAAAGACACTTAAGCCGCGTTACGCTGAGTACACGCCATTTAAAG
ATGATAATGGTTTAGAACTTGATCAAGGCATCGCCCTGTATTTTCCTAATCCACATTCTTTTACTGGTGAAGATGTTCTA
GAGCTTCAAGGACATGGCGGTCCTGTCGTTATGGATATGTTGATTAAACGCATCTTAAAAATTGAAGGGATCCGAACGGC
TCGCCCAGGAGAGTTCTCAGAAAGAGCTTTTTTGAACGATAAAATGGATTTAGCCCAAGCCGAAGCCATTGCCGATTTAA
TTGATGCAAGTTCAGAAGAAGCAGCAAAATCAGCACTTAATTCATTACAAGGTGTTTTCTCTAGAAAAATAAACACCTTA
GTTGAATCACTTATTTATCTGCGTATCTACGTTGAAGCTGCGATAGATTTCCCAGAAGAAGAAATCGACTTTCTGGCGGA
TGGCAAAGTGAGTGGTGACTTACAAGGCATTATTGATAACCTTAACGCGGTTCGCCAAGAAGCGAATCAAGGAGCCATTA
TGCGTGAAGGCATGAAGGTAGTTATTGCAGGTCGCCCAAACGCAGGTAAATCGAGTCTTCTGAACGCATTATCAGGCAAA
GACAGCGCTATCGTTACTGATATCGCAGGCACAACCCGTGATGTCTTGAGAGAGCATATTCACATTGATGGTATGCCATT
GCATATTATTGATACCGCAGGTCTTCGAGAAGCGTCAGATGAAGTAGAACGTATTGGTATAGAACGCGCGTGGCAAGAAA
TAGCCCAAGCAGACCGAGTTTTATTTATGGTTGATGGCACAACAACAGATGCAACTGACCCTAAAGTTATCTGGCCTGAA
TTTCTAGATCGATTGCCTAAGAATATGGGTATGACAGTCATTCGAAATAAAGCCGATCAAACCGGTGAAAATCTTGGTAT
TTGCCACGTTAACGATCCTACTCTTATTCGCTTATCAGCAAAAACAGGTGAAGGTGTTGATGCTTTGCGATTGCATTTAA
AAGAATGCATGGGTTTCTCTGGCAATCAAGAAGGTGGCTTTATGGCACGCAGACGTCATCTCGAAGCATTAGAAAAAGCA
TCTGAACACCTTGATATCGGTCAGCAACAACTTGAAGGTTATATGGCAGGAGAAATCCTGGCTGAAGAACTAAGAATCGC
TCAACAACACCTTAGTGAAATAACCGGTGAATTCAGTTCTGATGACTTATTAGGGCGGATTTTTACTTCTTTCTGTATTG
GTAAATAA

Upstream 100 bases:

>100_bases
AAGTTAACTTTCTAAAGAAATAAACAAAAGGCGGCCATTGGTCGCCTTTTTTGTTTTATCGGCTTACAATATGCTCAAGC
AATAGACATTAAGAATAATC

Downstream 100 bases:

>100_bases
ATCCTTCCAGCATAAACACAATCAATAATGAGCTATTACGGCCCATTTTTTATAGGAAAAATTCATGAAAAACGCATCAA
TTATTACAGGTAGCACCCTT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTLHTDTIVAQATAPGRGGVGIIRVSGPKAQQVALEVVGKTLKPRYAEYTPFKDDNGLELDQGIALYFPNPHSFTGEDVL
ELQGHGGPVVMDMLIKRILKIEGIRTARPGEFSERAFLNDKMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSRKINTL
VESLIYLRIYVEAAIDFPEEEIDFLADGKVSGDLQGIIDNLNAVRQEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGK
DSAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIAQADRVLFMVDGTTTDATDPKVIWPE
FLDRLPKNMGMTVIRNKADQTGENLGICHVNDPTLIRLSAKTGEGVDALRLHLKECMGFSGNQEGGFMARRRHLEALEKA
SEHLDIGQQQLEGYMAGEILAEELRIAQQHLSEITGEFSSDDLLGRIFTSFCIGK

Sequences:

>Translated_455_residues
MTLHTDTIVAQATAPGRGGVGIIRVSGPKAQQVALEVVGKTLKPRYAEYTPFKDDNGLELDQGIALYFPNPHSFTGEDVL
ELQGHGGPVVMDMLIKRILKIEGIRTARPGEFSERAFLNDKMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSRKINTL
VESLIYLRIYVEAAIDFPEEEIDFLADGKVSGDLQGIIDNLNAVRQEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGK
DSAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIAQADRVLFMVDGTTTDATDPKVIWPE
FLDRLPKNMGMTVIRNKADQTGENLGICHVNDPTLIRLSAKTGEGVDALRLHLKECMGFSGNQEGGFMARRRHLEALEKA
SEHLDIGQQQLEGYMAGEILAEELRIAQQHLSEITGEFSSDDLLGRIFTSFCIGK
>Mature_454_residues
TLHTDTIVAQATAPGRGGVGIIRVSGPKAQQVALEVVGKTLKPRYAEYTPFKDDNGLELDQGIALYFPNPHSFTGEDVLE
LQGHGGPVVMDMLIKRILKIEGIRTARPGEFSERAFLNDKMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSRKINTLV
ESLIYLRIYVEAAIDFPEEEIDFLADGKVSGDLQGIIDNLNAVRQEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGKD
SAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIAQADRVLFMVDGTTTDATDPKVIWPEF
LDRLPKNMGMTVIRNKADQTGENLGICHVNDPTLIRLSAKTGEGVDALRLHLKECMGFSGNQEGGFMARRRHLEALEKAS
EHLDIGQQQLEGYMAGEILAEELRIAQQHLSEITGEFSSDDLLGRIFTSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=34.8290598290598, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI193082988, Length=498, Percent_Identity=32.7309236947791, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI193082993, Length=458, Percent_Identity=33.6244541484716, Blast_Score=217, Evalue=2e-56,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=76.7184035476718, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=92, Percent_Identity=38.0434782608696, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=29.6536796536797, Blast_Score=168, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6323665, Length=501, Percent_Identity=31.7365269461078, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45550813, Length=491, Percent_Identity=34.0122199592668, Blast_Score=218, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49695; Mature: 49564

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHTDTIVAQATAPGRGGVGIIRVSGPKAQQVALEVVGKTLKPRYAEYTPFKDDNGLEL
CCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DQGIALYFPNPHSFTGEDVLELQGHGGPVVMDMLIKRILKIEGIRTARPGEFSERAFLND
CCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
KMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSRKINTLVESLIYLRIYVEAAIDFPEE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EIDFLADGKVSGDLQGIIDNLNAVRQEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCHHHHHHHHCCC
DSAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIAQADRV
CCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCE
LFMVDGTTTDATDPKVIWPEFLDRLPKNMGMTVIRNKADQTGENLGICHVNDPTLIRLSA
EEEEECCCCCCCCCCEECHHHHHHCHHHCCCHHEECCCCCCCCCEEEEECCCCEEEEEEC
KTGEGVDALRLHLKECMGFSGNQEGGFMARRRHLEALEKASEHLDIGQQQLEGYMAGEIL
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AEELRIAQQHLSEITGEFSSDDLLGRIFTSFCIGK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLHTDTIVAQATAPGRGGVGIIRVSGPKAQQVALEVVGKTLKPRYAEYTPFKDDNGLEL
CCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DQGIALYFPNPHSFTGEDVLELQGHGGPVVMDMLIKRILKIEGIRTARPGEFSERAFLND
CCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
KMDLAQAEAIADLIDASSEEAAKSALNSLQGVFSRKINTLVESLIYLRIYVEAAIDFPEE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EIDFLADGKVSGDLQGIIDNLNAVRQEANQGAIMREGMKVVIAGRPNAGKSSLLNALSGK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCHHHHHHHHCCC
DSAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIAQADRV
CCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCE
LFMVDGTTTDATDPKVIWPEFLDRLPKNMGMTVIRNKADQTGENLGICHVNDPTLIRLSA
EEEEECCCCCCCCCCEECHHHHHHCHHHCCCHHEECCCCCCCCCEEEEECCCCEEEEEEC
KTGEGVDALRLHLKECMGFSGNQEGGFMARRRHLEALEKASEHLDIGQQQLEGYMAGEIL
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AEELRIAQQHLSEITGEFSSDDLLGRIFTSFCIGK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA