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The map label for this gene is topA [H]

Identifier: 203284728

GI number: 203284728

Start: 896644

End: 899181

Strand: Reverse

Name: topA [H]

Synonym: BDU_840

Alternate gene names: 203284728

Gene position: 899181-896644 (Counterclockwise)

Preceding gene: 203284733

Following gene: 203284727

Centisome position: 96.51

GC content: 27.07

Gene sequence:

>2538_bases
ATGCAACAAAAAAGATTAATAATAGTAGAATCACCAACAAAAGCAACAACAATAAAAAAATATCTAGACAGCTCATTTTC
TGTGGAGGCTTGTATAGGGCATATAAGAGACTTGCCAAACAGCGCCAAAGAAATACCAGAAGAATATAAAAAATTTGAAT
GGGCAAATCTGTCGATAGATTACCAAAATGATTTTAAGCCACTCTATATTATTCCAAGTAATAAAAAAATGATTATTGAA
AAACTTAAAAAACTCATAAAAGACCAAGATGAAATATATCTTGCAACTGACCAAGACAGAGAAGGCGAAACAATAGCATT
TCACTTAAAAGAAGTATTACAAATCAAAAATTACAAAAGAATGGTATTCCACGAAATTACAAAAAGCGCAATAATTGACT
CACTAAATAATACAAGAGAAATTAATATGAATTTAGTTAATGCTGGTGAAACAAGAAGAATACTCGATAGACTTTATGGA
TATACTATTTCTCCTTTACTTTGGAAAAAAATTGCCTATGGACTTTCTGCTGGCAGAGTACAATCTGTAGGACTTCAATT
AATTATAGAAAAAGAAAAATCAAGAATAAATTTTAAAAAAGCTAACTATTACTCAATTACACTTGAATGTAAACATAACA
AAAACAATATACAACTTAAACCAACATTAGAAAAAATTGACAATAAAAATGTAGCCGAAAGTAAAGACTTTGAAAATGAT
ACAGGAACTTTACATGAGCCTAATAAAACAATCCTTATAGACAAAATATTAATGAATTCAATCACAAAAGAGCTAAAACA
AATAAACAATATAGAAGTAATTTCAATTGAAACAAAAGAAATCAAACTTACTCCTCCAAAGCCATTCATAACATCATCAC
TACAACAAGAAATAAACAGACGATTAAAAATAGGAACAAAACAAATTATGAGTTATGCACAAAAACTATATGAAAGCGGA
TACATAACCTATATGAGAACAGACTCTTATGAGATTGCAAAAACTGCAAAAGAAAAAATAAAAAACATTATAAGACAAAA
ATATGGAGAGGAATATATAGAAAAAAATGACAGAATGTATACCAAGGCTAAAATGGCTCAAGATGCACATGAAGCCATCA
GACCTTCTGAAATGTTCACTCCTCCTGAACAAATACAAATTGAAAGCGAAACAGCTAAAGAAATATATAAAATAATATGG
GATAGAACCATTGAATCTTGTATGAAAGATGCAATAAAAAAAATGGGAAAAATAAAATTTAAATATAAAAATTTAACCTT
CAAAGCAAATTTTACAAAACTTATTTTTGATGGCTTCTTGAAAAATAATAAAGAATTTCATGAAGATAACATAATAGATT
TTTCACTTATAAAAGAAGGAGATAAATTCACTATATTGAATATAAAACCAGAACAACATGAAACAAAACCCCCATTAAGA
TATACAGAAGCATCACTTGTACAAAAACTTGAAAAAGAAGGAATAGGTCGTCCTTCAACTTATGCAACAATAATATCAAC
ACTATTTGAAAGAGAATATGTATTTAAACAAAATAACACATTAATACCAACAATAAAAGGAGCTGCAGTCATAAATCTTT
TAAAAAAATATTTTCCAATACTAATTGAATTGCAATTCACATCTAATATGGAAGAAAATCTAGACAAAATTGCAATAGGC
AAATTAGATAAAATACAATATCTAAATGCATTTTATAATGGAAAAGAAGGTCTCAAGAACACAGTAAGTAAAATAGAACC
TATGATCAGTCCTGAAGAATTTAGAATGGTATATGAATATAATAATGACAAACAATTTAATTACAAAATTAATATTGGAA
AATTCGGGCCATACTTAATTTTTAATGGAAATAATTATGCAATAAATTTAACAACACCTCTCGAAAATTTGTATAAAAAA
GATGAAATACAAAAAATAATAACAGAAAAAGAAATACAACCTAATATATTAGGGGTTGATCCTGCAACAGGACTTAACAT
AATATATAAAAATTCAAAATACGGAGGAATTATCCAACTCGGCGAAGACATTTATGAACCACAAGGATTTACAAAAACAG
GCAAACCAAAAAAACCTAAAATAATTAAAGCTAAAAAAGTTTCAACAAAAAACATAGAATATGAAAATATCACGCTAGAA
CTTGCTTTACAATTGCTCTCCCTTCCAAAAGTAATAGGCAAATATCCAAAAACCAATGAAAAAATAATTGCTACTACAGG
AATCTTTGGAGACTATATTAAAACTGAAAACGATAGCATAATATGTCCACTAAAAAAAGACATGAAAGCATATAAAATCA
CACTCGAAGAATCAATTAAATTACTAGACACAAAATTAGCAAAAAACGGAATTATTGTAAAAATAATATCTCTATCTAAA
AACAAAATTGGAAATAAAATCTATATTTACAAACAAAATGACAAATTTTATGCTAAAATTAAAAGGAAAAAGGTTGATTT
GCCTACTAATATCGACCTTAATCAAATAGATGCAACTTATGTTTTTGGACTAATGTAG

Upstream 100 bases:

>100_bases
ATGTAAAATTTTATAGATACTCATAAAATACCGTGCTATTTTTGTTGAAAAATTATAACAATTTATACATTATGTTATAT
TATATATATATAAGGGTTTT

Downstream 100 bases:

>100_bases
GATATGAATGATTTTGGACTCCCCATTTACAAATACAAAGATGACTTAATTAAAAAATTAAGCACAAATAGTGTCTTGAT
AGTAGAAAGTCCAACAGGAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 845; Mature: 845

Protein sequence:

>845_residues
MQQKRLIIVESPTKATTIKKYLDSSFSVEACIGHIRDLPNSAKEIPEEYKKFEWANLSIDYQNDFKPLYIIPSNKKMIIE
KLKKLIKDQDEIYLATDQDREGETIAFHLKEVLQIKNYKRMVFHEITKSAIIDSLNNTREINMNLVNAGETRRILDRLYG
YTISPLLWKKIAYGLSAGRVQSVGLQLIIEKEKSRINFKKANYYSITLECKHNKNNIQLKPTLEKIDNKNVAESKDFEND
TGTLHEPNKTILIDKILMNSITKELKQINNIEVISIETKEIKLTPPKPFITSSLQQEINRRLKIGTKQIMSYAQKLYESG
YITYMRTDSYEIAKTAKEKIKNIIRQKYGEEYIEKNDRMYTKAKMAQDAHEAIRPSEMFTPPEQIQIESETAKEIYKIIW
DRTIESCMKDAIKKMGKIKFKYKNLTFKANFTKLIFDGFLKNNKEFHEDNIIDFSLIKEGDKFTILNIKPEQHETKPPLR
YTEASLVQKLEKEGIGRPSTYATIISTLFEREYVFKQNNTLIPTIKGAAVINLLKKYFPILIELQFTSNMEENLDKIAIG
KLDKIQYLNAFYNGKEGLKNTVSKIEPMISPEEFRMVYEYNNDKQFNYKINIGKFGPYLIFNGNNYAINLTTPLENLYKK
DEIQKIITEKEIQPNILGVDPATGLNIIYKNSKYGGIIQLGEDIYEPQGFTKTGKPKKPKIIKAKKVSTKNIEYENITLE
LALQLLSLPKVIGKYPKTNEKIIATTGIFGDYIKTENDSIICPLKKDMKAYKITLEESIKLLDTKLAKNGIIVKIISLSK
NKIGNKIYIYKQNDKFYAKIKRKKVDLPTNIDLNQIDATYVFGLM

Sequences:

>Translated_845_residues
MQQKRLIIVESPTKATTIKKYLDSSFSVEACIGHIRDLPNSAKEIPEEYKKFEWANLSIDYQNDFKPLYIIPSNKKMIIE
KLKKLIKDQDEIYLATDQDREGETIAFHLKEVLQIKNYKRMVFHEITKSAIIDSLNNTREINMNLVNAGETRRILDRLYG
YTISPLLWKKIAYGLSAGRVQSVGLQLIIEKEKSRINFKKANYYSITLECKHNKNNIQLKPTLEKIDNKNVAESKDFEND
TGTLHEPNKTILIDKILMNSITKELKQINNIEVISIETKEIKLTPPKPFITSSLQQEINRRLKIGTKQIMSYAQKLYESG
YITYMRTDSYEIAKTAKEKIKNIIRQKYGEEYIEKNDRMYTKAKMAQDAHEAIRPSEMFTPPEQIQIESETAKEIYKIIW
DRTIESCMKDAIKKMGKIKFKYKNLTFKANFTKLIFDGFLKNNKEFHEDNIIDFSLIKEGDKFTILNIKPEQHETKPPLR
YTEASLVQKLEKEGIGRPSTYATIISTLFEREYVFKQNNTLIPTIKGAAVINLLKKYFPILIELQFTSNMEENLDKIAIG
KLDKIQYLNAFYNGKEGLKNTVSKIEPMISPEEFRMVYEYNNDKQFNYKINIGKFGPYLIFNGNNYAINLTTPLENLYKK
DEIQKIITEKEIQPNILGVDPATGLNIIYKNSKYGGIIQLGEDIYEPQGFTKTGKPKKPKIIKAKKVSTKNIEYENITLE
LALQLLSLPKVIGKYPKTNEKIIATTGIFGDYIKTENDSIICPLKKDMKAYKITLEESIKLLDTKLAKNGIIVKIISLSK
NKIGNKIYIYKQNDKFYAKIKRKKVDLPTNIDLNQIDATYVFGLM
>Mature_845_residues
MQQKRLIIVESPTKATTIKKYLDSSFSVEACIGHIRDLPNSAKEIPEEYKKFEWANLSIDYQNDFKPLYIIPSNKKMIIE
KLKKLIKDQDEIYLATDQDREGETIAFHLKEVLQIKNYKRMVFHEITKSAIIDSLNNTREINMNLVNAGETRRILDRLYG
YTISPLLWKKIAYGLSAGRVQSVGLQLIIEKEKSRINFKKANYYSITLECKHNKNNIQLKPTLEKIDNKNVAESKDFEND
TGTLHEPNKTILIDKILMNSITKELKQINNIEVISIETKEIKLTPPKPFITSSLQQEINRRLKIGTKQIMSYAQKLYESG
YITYMRTDSYEIAKTAKEKIKNIIRQKYGEEYIEKNDRMYTKAKMAQDAHEAIRPSEMFTPPEQIQIESETAKEIYKIIW
DRTIESCMKDAIKKMGKIKFKYKNLTFKANFTKLIFDGFLKNNKEFHEDNIIDFSLIKEGDKFTILNIKPEQHETKPPLR
YTEASLVQKLEKEGIGRPSTYATIISTLFEREYVFKQNNTLIPTIKGAAVINLLKKYFPILIELQFTSNMEENLDKIAIG
KLDKIQYLNAFYNGKEGLKNTVSKIEPMISPEEFRMVYEYNNDKQFNYKINIGKFGPYLIFNGNNYAINLTTPLENLYKK
DEIQKIITEKEIQPNILGVDPATGLNIIYKNSKYGGIIQLGEDIYEPQGFTKTGKPKKPKIIKAKKVSTKNIEYENITLE
LALQLLSLPKVIGKYPKTNEKIIATTGIFGDYIKTENDSIICPLKKDMKAYKITLEESIKLLDTKLAKNGIIVKIISLSK
NKIGNKIYIYKQNDKFYAKIKRKKVDLPTNIDLNQIDATYVFGLM

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=549, Percent_Identity=22.4043715846995, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI4507635, Length=513, Percent_Identity=24.1715399610136, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1787529, Length=590, Percent_Identity=36.1016949152542, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32563869, Length=569, Percent_Identity=22.1441124780316, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17555378, Length=525, Percent_Identity=21.9047619047619, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323263, Length=600, Percent_Identity=21, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97896; Mature: 97896

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQKRLIIVESPTKATTIKKYLDSSFSVEACIGHIRDLPNSAKEIPEEYKKFEWANLSID
CCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCEEEEEEEE
YQNDFKPLYIIPSNKKMIIEKLKKLIKDQDEIYLATDQDREGETIAFHLKEVLQIKNYKR
ECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHH
MVFHEITKSAIIDSLNNTREINMNLVNAGETRRILDRLYGYTISPLLWKKIAYGLSAGRV
HHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCH
QSVGLQLIIEKEKSRINFKKANYYSITLECKHNKNNIQLKPTLEKIDNKNVAESKDFEND
HHCCEEEEEEECCCCCEEEECEEEEEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCCC
TGTLHEPNKTILIDKILMNSITKELKQINNIEVISIETKEIKLTPPKPFITSSLQQEINR
CCCEECCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEEECEEEECCCCCHHHHHHHHHHHH
RLKIGTKQIMSYAQKLYESGYITYMRTDSYEIAKTAKEKIKNIIRQKYGEEYIEKNDRMY
HHHCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEE
TKAKMAQDAHEAIRPSEMFTPPEQIQIESETAKEIYKIIWDRTIESCMKDAIKKMGKIKF
HHHHHHHHHHHHCCCHHCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
KYKNLTFKANFTKLIFDGFLKNNKEFHEDNIIDFSLIKEGDKFTILNIKPEQHETKPPLR
EEEEEEEEECHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCC
YTEASLVQKLEKEGIGRPSTYATIISTLFEREYVFKQNNTLIPTIKGAAVINLLKKYFPI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCEEEEEECHHHHHHHHHHHCCE
LIELQFTSNMEENLDKIAIGKLDKIQYLNAFYNGKEGLKNTVSKIEPMISPEEFRMVYEY
EEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEE
NNDKQFNYKINIGKFGPYLIFNGNNYAINLTTPLENLYKKDEIQKIITEKEIQPNILGVD
CCCCEEEEEEEEECCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEECC
PATGLNIIYKNSKYGGIIQLGEDIYEPQGFTKTGKPKKPKIIKAKKVSTKNIEYENITLE
CCCCCEEEEECCCCCCEEEECHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCHHH
LALQLLSLPKVIGKYPKTNEKIIATTGIFGDYIKTENDSIICPLKKDMKAYKITLEESIK
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEECCCCCEEECCHHCCEEEEEEHHHHHH
LLDTKLAKNGIIVKIISLSKNKIGNKIYIYKQNDKFYAKIKRKKVDLPTNIDLNQIDATY
HHHHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEEHHCCCCCCCCCCCCCCCEE
VFGLM
EEECC
>Mature Secondary Structure
MQQKRLIIVESPTKATTIKKYLDSSFSVEACIGHIRDLPNSAKEIPEEYKKFEWANLSID
CCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCEEEEEEEE
YQNDFKPLYIIPSNKKMIIEKLKKLIKDQDEIYLATDQDREGETIAFHLKEVLQIKNYKR
ECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHH
MVFHEITKSAIIDSLNNTREINMNLVNAGETRRILDRLYGYTISPLLWKKIAYGLSAGRV
HHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCH
QSVGLQLIIEKEKSRINFKKANYYSITLECKHNKNNIQLKPTLEKIDNKNVAESKDFEND
HHCCEEEEEEECCCCCEEEECEEEEEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCCC
TGTLHEPNKTILIDKILMNSITKELKQINNIEVISIETKEIKLTPPKPFITSSLQQEINR
CCCEECCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEEECEEEECCCCCHHHHHHHHHHHH
RLKIGTKQIMSYAQKLYESGYITYMRTDSYEIAKTAKEKIKNIIRQKYGEEYIEKNDRMY
HHHCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEE
TKAKMAQDAHEAIRPSEMFTPPEQIQIESETAKEIYKIIWDRTIESCMKDAIKKMGKIKF
HHHHHHHHHHHHCCCHHCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
KYKNLTFKANFTKLIFDGFLKNNKEFHEDNIIDFSLIKEGDKFTILNIKPEQHETKPPLR
EEEEEEEEECHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCC
YTEASLVQKLEKEGIGRPSTYATIISTLFEREYVFKQNNTLIPTIKGAAVINLLKKYFPI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCEEEEEECHHHHHHHHHHHCCE
LIELQFTSNMEENLDKIAIGKLDKIQYLNAFYNGKEGLKNTVSKIEPMISPEEFRMVYEY
EEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEE
NNDKQFNYKINIGKFGPYLIFNGNNYAINLTTPLENLYKKDEIQKIITEKEIQPNILGVD
CCCCEEEEEEEEECCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEECC
PATGLNIIYKNSKYGGIIQLGEDIYEPQGFTKTGKPKKPKIIKAKKVSTKNIEYENITLE
CCCCCEEEEECCCCCCEEEECHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCHHH
LALQLLSLPKVIGKYPKTNEKIIATTGIFGDYIKTENDSIICPLKKDMKAYKITLEESIK
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEECCCCCEEECCHHCCEEEEEEHHHHHH
LLDTKLAKNGIIVKIISLSKNKIGNKIYIYKQNDKFYAKIKRKKVDLPTNIDLNQIDATY
HHHHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEEHHCCCCCCCCCCCCCCCEE
VFGLM
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]