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The map label for this gene is nagE [H]

Identifier: 203284726

GI number: 203284726

Start: 892627

End: 894063

Strand: Reverse

Name: nagE [H]

Synonym: BDU_838

Alternate gene names: 203284726

Gene position: 894063-892627 (Counterclockwise)

Preceding gene: 203284727

Following gene: 203284725

Centisome position: 95.96

GC content: 32.92

Gene sequence:

>1437_bases
ATGCATAATTTAATGAAAAATATGCAAAATCTTGGCAAAGCACTACAAACTCCTGCTGCCGTTTTACCAATTGCTGGTCT
TTTACTCGGATTTGGATATCTTATAATATCTCTTACACAACCTTTTGAAATACTAGGCAAAATTGGCAAACTAATGGAAC
AATCAGGTAGCGCTATTCTTGGCAACTTACCAATACTATTTGCAATTGGAACTGGAATGGGATTATCTAAAAATAACAAA
GCTGCTGCTGCACTAGGAGGAGCCGTAGGATATCTAATTCTAAACGCAGGATTATCTACATTTACAATAATTATCAATGA
AAAAACCGAGCCTGTTAACATGTCTGTATTAGGAGGTATCATTACAGGAATAAGTTCAGCTATGCTTAGCGATAAAGTAG
TAGATTATAAAATACCACAATTTTTAGGATTTTTTTCAGGACAAAGATTAGTACCAATAGCCAATGGAATCTTTTCTGTA
ATATTTGCTATAGTATTTGGCTTTTTATGGGCACCAATACAAATAACCATTAATCAAATTGGAATATGGATGATAGAAGC
TGGCAATTTGGGACTATTTGTATTTGGATTCTTAAACAGATTACTCATAATAACAGGATTACATCAACTCTTAAATACAT
TGGTGTATTTTGTATTTGGAGAATATACCACAAATGAGGGAAATATAGTACAAGGAGAAATCACAAGATATTTAAATGGA
GATCCAACAGCTGGAGGATTCACATCGGGAATGTATCCAATAATGTTATTTGGACTACCAGGTGCAGCTCTTGCAATGTA
CTTAACAGCTAAAAAAGAACGCAGACAAGAAATAGGAGGTATCCTGCTCTCAGCATCACTAACCTCATTTTTAACAGGCA
TTACAGAACCAATAGAATATACATTCATGTTAATAGCACCACTGCTTTACCTAATACATGCTATTTTAACTGGAATATCA
CTAATCATTACCAATATATTTGGAATACGCATAGCATTTGCACTCTCAGCAGGAATCATCGATTATTTTTTAATGTTCCC
AAAATCAACGAACGCACTATTAATACTTCCAATAGGACTGACAATTGGTATCATTTACTTCACCATATTTTTCACACTAA
TTAAAGCATTTCAAATAAAAACTCCTGGCCGTGAAGATGATATTAATCAACAAAATTTTAAACATATTCAAAATATATCT
CAAAATGATACTTTTGAGGTAATAATTCAAGCTCTTGGAGGCACTAACAATATAATAAATATAGACTCATGTTTCACACG
ACTCAGAGTAGACGTAAAATCGCCCGAATTAGTCAATCAAGATTTAATGCATAATCTTGGAGCCACTGGAACAATTATCA
CACCAGGAAATCAGGCTCAAATAATATTTGGTGCAAAATCAGAACAAATTGCAAATTACATAAAATCTAAAATTTAA

Upstream 100 bases:

>100_bases
CTTTTTTTAAAAAAAAAGTAAATAAATATTATTTAAGCTAGAATCATATTGAAAGTTGATCTACACTATAACTAACAAAT
AACATATTAATGGGGGTCAT

Downstream 100 bases:

>100_bases
AAAATAAAAAATACAAAGAAATAAATTTATTTCTTTCTTTGTATTTCTACTAAAAGGATATGATGCTTAGGATAAAATCA
AATGCCTTTAACATTATCTA

Product: Sugar Specific PTS family, n-acetylglucosamine-specific enzyme IIABC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MHNLMKNMQNLGKALQTPAAVLPIAGLLLGFGYLIISLTQPFEILGKIGKLMEQSGSAILGNLPILFAIGTGMGLSKNNK
AAAALGGAVGYLILNAGLSTFTIIINEKTEPVNMSVLGGIITGISSAMLSDKVVDYKIPQFLGFFSGQRLVPIANGIFSV
IFAIVFGFLWAPIQITINQIGIWMIEAGNLGLFVFGFLNRLLIITGLHQLLNTLVYFVFGEYTTNEGNIVQGEITRYLNG
DPTAGGFTSGMYPIMLFGLPGAALAMYLTAKKERRQEIGGILLSASLTSFLTGITEPIEYTFMLIAPLLYLIHAILTGIS
LIITNIFGIRIAFALSAGIIDYFLMFPKSTNALLILPIGLTIGIIYFTIFFTLIKAFQIKTPGREDDINQQNFKHIQNIS
QNDTFEVIIQALGGTNNIINIDSCFTRLRVDVKSPELVNQDLMHNLGATGTIITPGNQAQIIFGAKSEQIANYIKSKI

Sequences:

>Translated_478_residues
MHNLMKNMQNLGKALQTPAAVLPIAGLLLGFGYLIISLTQPFEILGKIGKLMEQSGSAILGNLPILFAIGTGMGLSKNNK
AAAALGGAVGYLILNAGLSTFTIIINEKTEPVNMSVLGGIITGISSAMLSDKVVDYKIPQFLGFFSGQRLVPIANGIFSV
IFAIVFGFLWAPIQITINQIGIWMIEAGNLGLFVFGFLNRLLIITGLHQLLNTLVYFVFGEYTTNEGNIVQGEITRYLNG
DPTAGGFTSGMYPIMLFGLPGAALAMYLTAKKERRQEIGGILLSASLTSFLTGITEPIEYTFMLIAPLLYLIHAILTGIS
LIITNIFGIRIAFALSAGIIDYFLMFPKSTNALLILPIGLTIGIIYFTIFFTLIKAFQIKTPGREDDINQQNFKHIQNIS
QNDTFEVIIQALGGTNNIINIDSCFTRLRVDVKSPELVNQDLMHNLGATGTIITPGNQAQIIFGAKSEQIANYIKSKI
>Mature_478_residues
MHNLMKNMQNLGKALQTPAAVLPIAGLLLGFGYLIISLTQPFEILGKIGKLMEQSGSAILGNLPILFAIGTGMGLSKNNK
AAAALGGAVGYLILNAGLSTFTIIINEKTEPVNMSVLGGIITGISSAMLSDKVVDYKIPQFLGFFSGQRLVPIANGIFSV
IFAIVFGFLWAPIQITINQIGIWMIEAGNLGLFVFGFLNRLLIITGLHQLLNTLVYFVFGEYTTNEGNIVQGEITRYLNG
DPTAGGFTSGMYPIMLFGLPGAALAMYLTAKKERRQEIGGILLSASLTSFLTGITEPIEYTFMLIAPLLYLIHAILTGIS
LIITNIFGIRIAFALSAGIIDYFLMFPKSTNALLILPIGLTIGIIYFTIFFTLIKAFQIKTPGREDDINQQNFKHIQNIS
QNDTFEVIIQALGGTNNIINIDSCFTRLRVDVKSPELVNQDLMHNLGATGTIITPGNQAQIIFGAKSEQIANYIKSKI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=482, Percent_Identity=45.643153526971, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787343, Length=487, Percent_Identity=37.3716632443532, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787908, Length=513, Percent_Identity=30.4093567251462, Blast_Score=229, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51787; Mature: 51787

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00083 INTRADIOL_DIOXYGENAS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLMKNMQNLGKALQTPAAVLPIAGLLLGFGYLIISLTQPFEILGKIGKLMEQSGSAIL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE
GNLPILFAIGTGMGLSKNNKAAAALGGAVGYLILNAGLSTFTIIINEKTEPVNMSVLGGI
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
ITGISSAMLSDKVVDYKIPQFLGFFSGQRLVPIANGIFSVIFAIVFGFLWAPIQITINQI
HHHHHHHHHHCCHHHEECHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
GIWMIEAGNLGLFVFGFLNRLLIITGLHQLLNTLVYFVFGEYTTNEGNIVQGEITRYLNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCEEEHHHHHHCCC
DPTAGGFTSGMYPIMLFGLPGAALAMYLTAKKERRQEIGGILLSASLTSFLTGITEPIEY
CCCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHEHHHHHHHHHHHHCCHHHHH
TFMLIAPLLYLIHAILTGISLIITNIFGIRIAFALSAGIIDYFLMFPKSTNALLILPIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHH
TIGIIYFTIFFTLIKAFQIKTPGREDDINQQNFKHIQNISQNDTFEVIIQALGGTNNIIN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEE
IDSCFTRLRVDVKSPELVNQDLMHNLGATGTIITPGNQAQIIFGAKSEQIANYIKSKI
HHHHHHHHEECCCCCHHHHHHHHHHCCCCCEEECCCCCEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MHNLMKNMQNLGKALQTPAAVLPIAGLLLGFGYLIISLTQPFEILGKIGKLMEQSGSAIL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE
GNLPILFAIGTGMGLSKNNKAAAALGGAVGYLILNAGLSTFTIIINEKTEPVNMSVLGGI
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
ITGISSAMLSDKVVDYKIPQFLGFFSGQRLVPIANGIFSVIFAIVFGFLWAPIQITINQI
HHHHHHHHHHCCHHHEECHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
GIWMIEAGNLGLFVFGFLNRLLIITGLHQLLNTLVYFVFGEYTTNEGNIVQGEITRYLNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCEEEHHHHHHCCC
DPTAGGFTSGMYPIMLFGLPGAALAMYLTAKKERRQEIGGILLSASLTSFLTGITEPIEY
CCCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHEHHHHHHHHHHHHCCHHHHH
TFMLIAPLLYLIHAILTGISLIITNIFGIRIAFALSAGIIDYFLMFPKSTNALLILPIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHH
TIGIIYFTIFFTLIKAFQIKTPGREDDINQQNFKHIQNISQNDTFEVIIQALGGTNNIIN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEE
IDSCFTRLRVDVKSPELVNQDLMHNLGATGTIITPGNQAQIIFGAKSEQIANYIKSKI
HHHHHHHHEECCCCCHHHHHHHHHHCCCCCEEECCCCCEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]