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The map label for this gene is rpoS [H]

Identifier: 203284664

GI number: 203284664

Start: 827814

End: 828617

Strand: Reverse

Name: rpoS [H]

Synonym: BDU_775

Alternate gene names: 203284664

Gene position: 828617-827814 (Counterclockwise)

Preceding gene: 203284665

Following gene: 203284663

Centisome position: 88.94

GC content: 26.87

Gene sequence:

>804_bases
GTGAATATATTTAGCAATGAAGATTTAAATATATATCTAAAATCTGTAAGAGAACATAGACTTATAACTCACGAAGAAGA
AATCGAACTTGCAAAACAGATAAAACTAGGAAGCTTAAAAGCAAAAAACCAAATGATAAATGCCAACTTACGTTTAGTTC
TCAAAATAATTAAAAGATATGCTGGTAAAGGATTAAAAATAGAAGATCTAATACAAGAAGGAAATTTAGGTTTAATTAGA
GCTGCAGAAAAATATGATCCGAGTAAAAATACCAAATTTTCAACTTATGCTTCATTTTGGATTAAACAATCTCTTCAAAG
GGCATTAAACACAAAAACAAGACTTGTAAAAGTACCATACAGAAAAGAAAATTTAATACTTCAAATCAATAAATATCTAA
TGGAAGAAGAAAAATCACCCAAAAAAGAAGACATAATGGAAAGATTTAACTTGACAACTGCTCAATATGTAAAGATTATT
CCTTACCTTGAAAAAGAATATTCTCTAGATAAAAAGATTGAAGGATCAGAAAATTCCACGCTACTAAATCTCTATGAAGA
TAATTCTTTTAATCCTGAGAGTACTCTTGAACAAAATTCAACATTAAAGCATCTAAACTATATACTAGAAACTAAACTAA
ATGAAAAAGAAAGATATATAATCAAGAAAAGATACAATTTGGACAATAGTGATAAAAAAAGCACATTAAAAGATATTTCT
ACAGAACTTGGAATATCTTCAGAAACTGTAAGACAGATCGAAAAAAGAGTACTAAAAAAACTCAGAGAAGAACTTTATCA
ATAA

Upstream 100 bases:

>100_bases
TAAAAATATCAATTATTTTCTAAGTTGGCATGATTTTTGCATCACATCAAAAAGGGAGAAGGATGTTGATACACAAAATT
TAAAAGATGGGAGAAGTAGT

Downstream 100 bases:

>100_bases
ATATTGACTTAGTCACATATTTAAATATAATAAGAATTTCTGAGAATGAATATTCAAAATATTTATGTTTTTATTAAAAG
ACATAAGTTACAAATTAAAG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNIFSNEDLNIYLKSVREHRLITHEEEIELAKQIKLGSLKAKNQMINANLRLVLKIIKRYAGKGLKIEDLIQEGNLGLIR
AAEKYDPSKNTKFSTYASFWIKQSLQRALNTKTRLVKVPYRKENLILQINKYLMEEEKSPKKEDIMERFNLTTAQYVKII
PYLEKEYSLDKKIEGSENSTLLNLYEDNSFNPESTLEQNSTLKHLNYILETKLNEKERYIIKKRYNLDNSDKKSTLKDIS
TELGISSETVRQIEKRVLKKLREELYQ

Sequences:

>Translated_267_residues
MNIFSNEDLNIYLKSVREHRLITHEEEIELAKQIKLGSLKAKNQMINANLRLVLKIIKRYAGKGLKIEDLIQEGNLGLIR
AAEKYDPSKNTKFSTYASFWIKQSLQRALNTKTRLVKVPYRKENLILQINKYLMEEEKSPKKEDIMERFNLTTAQYVKII
PYLEKEYSLDKKIEGSENSTLLNLYEDNSFNPESTLEQNSTLKHLNYILETKLNEKERYIIKKRYNLDNSDKKSTLKDIS
TELGISSETVRQIEKRVLKKLREELYQ
>Mature_267_residues
MNIFSNEDLNIYLKSVREHRLITHEEEIELAKQIKLGSLKAKNQMINANLRLVLKIIKRYAGKGLKIEDLIQEGNLGLIR
AAEKYDPSKNTKFSTYASFWIKQSLQRALNTKTRLVKVPYRKENLILQINKYLMEEEKSPKKEDIMERFNLTTAQYVKII
PYLEKEYSLDKKIEGSENSTLLNLYEDNSFNPESTLEQNSTLKHLNYILETKLNEKERYIIKKRYNLDNSDKKSTLKDIS
TELGISSETVRQIEKRVLKKLREELYQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=263, Percent_Identity=34.9809885931559, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1789448, Length=258, Percent_Identity=29.8449612403101, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=27.112676056338, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31499; Mature: 31499

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFSNEDLNIYLKSVREHRLITHEEEIELAKQIKLGSLKAKNQMINANLRLVLKIIKRY
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AGKGLKIEDLIQEGNLGLIRAAEKYDPSKNTKFSTYASFWIKQSLQRALNTKTRLVKVPY
CCCCCCHHHHHHCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
RKENLILQINKYLMEEEKSPKKEDIMERFNLTTAQYVKIIPYLEKEYSLDKKIEGSENST
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LLNLYEDNSFNPESTLEQNSTLKHLNYILETKLNEKERYIIKKRYNLDNSDKKSTLKDIS
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
TELGISSETVRQIEKRVLKKLREELYQ
HHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIFSNEDLNIYLKSVREHRLITHEEEIELAKQIKLGSLKAKNQMINANLRLVLKIIKRY
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AGKGLKIEDLIQEGNLGLIRAAEKYDPSKNTKFSTYASFWIKQSLQRALNTKTRLVKVPY
CCCCCCHHHHHHCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
RKENLILQINKYLMEEEKSPKKEDIMERFNLTTAQYVKIIPYLEKEYSLDKKIEGSENST
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LLNLYEDNSFNPESTLEQNSTLKHLNYILETKLNEKERYIIKKRYNLDNSDKKSTLKDIS
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
TELGISSETVRQIEKRVLKKLREELYQ
HHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]