Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 203284454

GI number: 203284454

Start: 573324

End: 576092

Strand: Reverse

Name: polA [H]

Synonym: BDU_550

Alternate gene names: 203284454

Gene position: 576092-573324 (Counterclockwise)

Preceding gene: 203284455

Following gene: 203284453

Centisome position: 61.83

GC content: 26.36

Gene sequence:

>2769_bases
ATGAAAGAAATTTACTTAATAGATGCATTAAACATAATTTTTAGAAACTATCACGTTATGAAAAACAATCCTTTAACAAA
TAGTAAAGGAGAAAATGTCAGCGCATTTATTGGTTTTTTTAAAACTCTTTTCTTTATCATAAAGGAAAAAAAACCAGAAA
ATCTAATTGTCACTTTTGATTCAGAAACACAAACATTTAGACAAAAACAATACCCAAGTTACAAAGCAACAAGAGATGCT
CCTCCAGACAACCTAATTCCACAAATTCATTGGATAAAAGAAGGATTAATAAAAGCAAATATCCCAATGTTTGAACTACA
AGGATATGAAGCTGATGATCTTATAGCCAGTTTTACAAAAAAGGCAGAACAAAACAATTACTTGACTTATATTATCTCTC
CAGATAAAGACTTATTACAATTAATATCAAATCAAACCAAAATATTTAAAATTGAAAATAGTAGCTTTTTAGAAATGAAC
AATGATTATGTTATAAAAAAATTTGGAATAAATAAATCTCAAATCAAAGACTATTTATCCATTGTTGGAGACCCCTCAGA
CAACATCCCAGGAATTAAAGGAATTGGACAAAAAGGTGCTTCTAAATTATTAAATGAATTTCACACATTAAATGGAATAT
ATGAAAACTTATCTTTAATAAATAATAAATACAGAGAAATCTTACTACGAGAAAAAGAAAATGCTTTTTTAAGTTATAAA
CTTATAAGTCTTGTAGAAGATCTAGAATTACCATCACTTGAAACATTTAGACTAGAAAATTTAAAAGAAGATATTATTTT
ACTATTTGAAGAATATTCTGCAACAACCCTCATTAAATCTTACAAATCTCTATTAAAGAAAAAAAATATTATAAATACAA
TGCAAAAATCAATCTTTGATATCAATAAAACAAAAGCAAATAAAGAAAATACAAAAGATTTACAATCAACAACATTATAT
GCAAATACTCTTAAGACAATACAAGAAGAAAATGTCAAATATAAAACAATATTAAAAAAAGAAGAACTGGATTTACTAAT
AGAACAACTAAAAAAAGCAAGCTATATAGCAATAGACACAGAAACAACTTCTATTAATGTTTATGAATCAAATATAATTG
GAATTTCAGTTTCATTTAAAGAATTTGAAAGTTACTATATTCCCATAGAAACCAAAGAAAAAAATTCTATTGGAAAAGAG
TACATAATACAAAAATTTAATGAATTGTTTAACTCACAACCAAAATTAATTGGTCAAAATTATAAATTTGATTACAAAGT
ACTAAAAAGACATGGATTTAATGTCATCTCTGCATATTTCGATACAATGATAGCAGCATATGTTATTGATCCAAACACAA
AAGTATCTCTTGACTTTTTAGCAGCAAAATATCTAATGCATAAAAACATTAAATACGAAGAAATAGTACCACAACATAGT
ACTCTTAAAGATATACCACTTGAAATAGCATCCAACTATGCTGCTGAAGATGCTGACATTACCTTCAGATTATTTAATAT
CTTGAGAAAAAAGCTCAAAGAAGACAATCTTGAAAGCCTAATGAAAAACATAGAAATGCCATTTAGCAATGTCATTATAG
AAATGGAAGAAAATGGCATTTACCTTGACAGCAATTACTTAACACAATATGGCCATGAACTTGAACAAGAATTAAAAACA
ATTGAGAATGAAGTAATACAAAGTATAGGCATTGAATTTAATCTAAATTCAAGCAAACAATTACATACGATTTTATTTGA
AAAATTAAATATTATAGTACCAAAAAATGTTAAGCAAGATTCAACAGATATCAAGGTGCTAGAGACAATAAAAGATCAGC
ATGAATCTATAGAAAAACTCATAAACTACAGACAACTTGCAAAACTAAAAAATACATATACCGATAATTTAATAGAATTT
ATAAACGAAAAAACAAACAAAATACATACGAACTTTATACAAACAAAAACAGCAACGGGACGAATTTCAAGTACTGCACC
CAATCTACAAAACATACCAATCAAAAATGAAAGAGGAAGAAAAATAAGAGCAGCATTCAAACCTACTAAAGGCAATATTT
TCATATCTGCAGATTATTCACAAATAGAACTTGTAATATTAGCACATCTCTCAGAAGACGAATCACTAATTGAAGCTTTT
ACACATAAAAAAGATATTCACATACAAACAGCATCAAAACTCTTTAAGGTAGATGAAGCCAACATAACACCTTCAATGAG
AAGAATAGCAAAATCAATTAATTTTGGAATAATTTATAGAATGTCAGCTTTTAGACTTTCACAAGAGTTATCCATTAAAA
GAGAGGAAGCACAAAATTTTATCGATTCATATTTTAATCTTTATTCTAAAATAAAAACTTTTATACAAAATCAAATAGAT
TTTGTAAAGCAAAATGGATACAGTGAAACCCTTTTAAAGAGAAGAAGATACATAAGAGAAATTAATAGTCAAAATTATTT
AGAAAGAACAGGTGCTGAACGAATGGCAATAAATAGTATAATTCAAGGAAGTGCATCTGATATAATGAAAATTGCAATGA
TTAAAGTATATAATGAATTTAAAAATAACAATCTAAAATCAAAAATACTCTTACAAGTACACGATGAAATGTTAATTGAA
TCACCAGAAGAAGAATGTGAAAAGGCACAAATAATAATAAAAGAAATGATGGAAAATGCTTATCCTTTAAAGATTCCTTT
AAAAACAAATATTGAAACTGGAAAATCATGGGGAGACATTCATCAATAA

Upstream 100 bases:

>100_bases
TGTTAAAAAAAATATTTGCTCAAAAAAAAGCAATACCAAAAATACAATTTTTCAAAAGTTCATTTAACCATAAAGAACCA
ATTTTATTAGATGTTATAAT

Downstream 100 bases:

>100_bases
TTGGAATAACTGGTAGAATATCAACCGGAAAAGACACTGTGTCAAAAATCATTAGTAACGAATATGATTTTTATGAAATT
AATGCAGATAAAATAGGTCA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 922; Mature: 922

Protein sequence:

>922_residues
MKEIYLIDALNIIFRNYHVMKNNPLTNSKGENVSAFIGFFKTLFFIIKEKKPENLIVTFDSETQTFRQKQYPSYKATRDA
PPDNLIPQIHWIKEGLIKANIPMFELQGYEADDLIASFTKKAEQNNYLTYIISPDKDLLQLISNQTKIFKIENSSFLEMN
NDYVIKKFGINKSQIKDYLSIVGDPSDNIPGIKGIGQKGASKLLNEFHTLNGIYENLSLINNKYREILLREKENAFLSYK
LISLVEDLELPSLETFRLENLKEDIILLFEEYSATTLIKSYKSLLKKKNIINTMQKSIFDINKTKANKENTKDLQSTTLY
ANTLKTIQEENVKYKTILKKEELDLLIEQLKKASYIAIDTETTSINVYESNIIGISVSFKEFESYYIPIETKEKNSIGKE
YIIQKFNELFNSQPKLIGQNYKFDYKVLKRHGFNVISAYFDTMIAAYVIDPNTKVSLDFLAAKYLMHKNIKYEEIVPQHS
TLKDIPLEIASNYAAEDADITFRLFNILRKKLKEDNLESLMKNIEMPFSNVIIEMEENGIYLDSNYLTQYGHELEQELKT
IENEVIQSIGIEFNLNSSKQLHTILFEKLNIIVPKNVKQDSTDIKVLETIKDQHESIEKLINYRQLAKLKNTYTDNLIEF
INEKTNKIHTNFIQTKTATGRISSTAPNLQNIPIKNERGRKIRAAFKPTKGNIFISADYSQIELVILAHLSEDESLIEAF
THKKDIHIQTASKLFKVDEANITPSMRRIAKSINFGIIYRMSAFRLSQELSIKREEAQNFIDSYFNLYSKIKTFIQNQID
FVKQNGYSETLLKRRRYIREINSQNYLERTGAERMAINSIIQGSASDIMKIAMIKVYNEFKNNNLKSKILLQVHDEMLIE
SPEEECEKAQIIIKEMMENAYPLKIPLKTNIETGKSWGDIHQ

Sequences:

>Translated_922_residues
MKEIYLIDALNIIFRNYHVMKNNPLTNSKGENVSAFIGFFKTLFFIIKEKKPENLIVTFDSETQTFRQKQYPSYKATRDA
PPDNLIPQIHWIKEGLIKANIPMFELQGYEADDLIASFTKKAEQNNYLTYIISPDKDLLQLISNQTKIFKIENSSFLEMN
NDYVIKKFGINKSQIKDYLSIVGDPSDNIPGIKGIGQKGASKLLNEFHTLNGIYENLSLINNKYREILLREKENAFLSYK
LISLVEDLELPSLETFRLENLKEDIILLFEEYSATTLIKSYKSLLKKKNIINTMQKSIFDINKTKANKENTKDLQSTTLY
ANTLKTIQEENVKYKTILKKEELDLLIEQLKKASYIAIDTETTSINVYESNIIGISVSFKEFESYYIPIETKEKNSIGKE
YIIQKFNELFNSQPKLIGQNYKFDYKVLKRHGFNVISAYFDTMIAAYVIDPNTKVSLDFLAAKYLMHKNIKYEEIVPQHS
TLKDIPLEIASNYAAEDADITFRLFNILRKKLKEDNLESLMKNIEMPFSNVIIEMEENGIYLDSNYLTQYGHELEQELKT
IENEVIQSIGIEFNLNSSKQLHTILFEKLNIIVPKNVKQDSTDIKVLETIKDQHESIEKLINYRQLAKLKNTYTDNLIEF
INEKTNKIHTNFIQTKTATGRISSTAPNLQNIPIKNERGRKIRAAFKPTKGNIFISADYSQIELVILAHLSEDESLIEAF
THKKDIHIQTASKLFKVDEANITPSMRRIAKSINFGIIYRMSAFRLSQELSIKREEAQNFIDSYFNLYSKIKTFIQNQID
FVKQNGYSETLLKRRRYIREINSQNYLERTGAERMAINSIIQGSASDIMKIAMIKVYNEFKNNNLKSKILLQVHDEMLIE
SPEEECEKAQIIIKEMMENAYPLKIPLKTNIETGKSWGDIHQ
>Mature_922_residues
MKEIYLIDALNIIFRNYHVMKNNPLTNSKGENVSAFIGFFKTLFFIIKEKKPENLIVTFDSETQTFRQKQYPSYKATRDA
PPDNLIPQIHWIKEGLIKANIPMFELQGYEADDLIASFTKKAEQNNYLTYIISPDKDLLQLISNQTKIFKIENSSFLEMN
NDYVIKKFGINKSQIKDYLSIVGDPSDNIPGIKGIGQKGASKLLNEFHTLNGIYENLSLINNKYREILLREKENAFLSYK
LISLVEDLELPSLETFRLENLKEDIILLFEEYSATTLIKSYKSLLKKKNIINTMQKSIFDINKTKANKENTKDLQSTTLY
ANTLKTIQEENVKYKTILKKEELDLLIEQLKKASYIAIDTETTSINVYESNIIGISVSFKEFESYYIPIETKEKNSIGKE
YIIQKFNELFNSQPKLIGQNYKFDYKVLKRHGFNVISAYFDTMIAAYVIDPNTKVSLDFLAAKYLMHKNIKYEEIVPQHS
TLKDIPLEIASNYAAEDADITFRLFNILRKKLKEDNLESLMKNIEMPFSNVIIEMEENGIYLDSNYLTQYGHELEQELKT
IENEVIQSIGIEFNLNSSKQLHTILFEKLNIIVPKNVKQDSTDIKVLETIKDQHESIEKLINYRQLAKLKNTYTDNLIEF
INEKTNKIHTNFIQTKTATGRISSTAPNLQNIPIKNERGRKIRAAFKPTKGNIFISADYSQIELVILAHLSEDESLIEAF
THKKDIHIQTASKLFKVDEANITPSMRRIAKSINFGIIYRMSAFRLSQELSIKREEAQNFIDSYFNLYSKIKTFIQNQID
FVKQNGYSETLLKRRRYIREINSQNYLERTGAERMAINSIIQGSASDIMKIAMIKVYNEFKNNNLKSKILLQVHDEMLIE
SPEEECEKAQIIIKEMMENAYPLKIPLKTNIETGKSWGDIHQ

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=516, Percent_Identity=31.0077519379845, Blast_Score=232, Evalue=1e-60,
Organism=Homo sapiens, GI139394648, Length=693, Percent_Identity=25.2525252525253, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1790294, Length=942, Percent_Identity=35.9872611464968, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI226510970, Length=258, Percent_Identity=31.0077519379845, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI86563272, Length=407, Percent_Identity=29.4840294840295, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI17933644, Length=427, Percent_Identity=31.3817330210773, Blast_Score=205, Evalue=1e-52,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 107028; Mature: 107028

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIYLIDALNIIFRNYHVMKNNPLTNSKGENVSAFIGFFKTLFFIIKEKKPENLIVTFD
CCEEEEEHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SETQTFRQKQYPSYKATRDAPPDNLIPQIHWIKEGLIKANIPMFELQGYEADDLIASFTK
CCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCHHCCCCCEEEECCCCHHHHHHHHHH
KAEQNNYLTYIISPDKDLLQLISNQTKIFKIENSSFLEMNNDYVIKKFGINKSQIKDYLS
HHCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCEEEECCCEEEEEECCCHHHHHHHHH
IVGDPSDNIPGIKGIGQKGASKLLNEFHTLNGIYENLSLINNKYREILLREKENAFLSYK
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LISLVEDLELPSLETFRLENLKEDIILLFEEYSATTLIKSYKSLLKKKNIINTMQKSIFD
HHHHHHHCCCCCCHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INKTKANKENTKDLQSTTLYANTLKTIQEENVKYKTILKKEELDLLIEQLKKASYIAIDT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
ETTSINVYESNIIGISVSFKEFESYYIPIETKEKNSIGKEYIIQKFNELFNSQPKLIGQN
CCCEEEEEECCEEEEEEEHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YKFDYKVLKRHGFNVISAYFDTMIAAYVIDPNTKVSLDFLAAKYLMHKNIKYEEIVPQHS
CCCHHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEHHHHHHHHHHHCCCCHHHHCCCCC
TLKDIPLEIASNYAAEDADITFRLFNILRKKLKEDNLESLMKNIEMPFSNVIIEMEENGI
CHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHEEEEEECCCE
YLDSNYLTQYGHELEQELKTIENEVIQSIGIEFNLNSSKQLHTILFEKLNIIVPKNVKQD
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCEEECCCCCCC
STDIKVLETIKDQHESIEKLINYRQLAKLKNTYTDNLIEFINEKTNKIHTNFIQTKTATG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHEEECCCCC
RISSTAPNLQNIPIKNERGRKIRAAFKPTKGNIFISADYSQIELVILAHLSEDESLIEAF
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCEEEEEEEEECCCCHHHHHHH
THKKDIHIQTASKLFKVDEANITPSMRRIAKSINFGIIYRMSAFRLSQELSIKREEAQNF
HCCCCEEEEHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHH
IDSYFNLYSKIKTFIQNQIDFVKQNGYSETLLKRRRYIREINSQNYLERTGAERMAINSI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHH
IQGSASDIMKIAMIKVYNEFKNNNLKSKILLQVHDEMLIESPEEECEKAQIIIKEMMENA
HCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
YPLKIPLKTNIETGKSWGDIHQ
CCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKEIYLIDALNIIFRNYHVMKNNPLTNSKGENVSAFIGFFKTLFFIIKEKKPENLIVTFD
CCEEEEEHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SETQTFRQKQYPSYKATRDAPPDNLIPQIHWIKEGLIKANIPMFELQGYEADDLIASFTK
CCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCHHCCCCCEEEECCCCHHHHHHHHHH
KAEQNNYLTYIISPDKDLLQLISNQTKIFKIENSSFLEMNNDYVIKKFGINKSQIKDYLS
HHCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCEEEECCCEEEEEECCCHHHHHHHHH
IVGDPSDNIPGIKGIGQKGASKLLNEFHTLNGIYENLSLINNKYREILLREKENAFLSYK
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LISLVEDLELPSLETFRLENLKEDIILLFEEYSATTLIKSYKSLLKKKNIINTMQKSIFD
HHHHHHHCCCCCCHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INKTKANKENTKDLQSTTLYANTLKTIQEENVKYKTILKKEELDLLIEQLKKASYIAIDT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
ETTSINVYESNIIGISVSFKEFESYYIPIETKEKNSIGKEYIIQKFNELFNSQPKLIGQN
CCCEEEEEECCEEEEEEEHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YKFDYKVLKRHGFNVISAYFDTMIAAYVIDPNTKVSLDFLAAKYLMHKNIKYEEIVPQHS
CCCHHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEHHHHHHHHHHHCCCCHHHHCCCCC
TLKDIPLEIASNYAAEDADITFRLFNILRKKLKEDNLESLMKNIEMPFSNVIIEMEENGI
CHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHEEEEEECCCE
YLDSNYLTQYGHELEQELKTIENEVIQSIGIEFNLNSSKQLHTILFEKLNIIVPKNVKQD
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCEEECCCCCCC
STDIKVLETIKDQHESIEKLINYRQLAKLKNTYTDNLIEFINEKTNKIHTNFIQTKTATG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHEEECCCCC
RISSTAPNLQNIPIKNERGRKIRAAFKPTKGNIFISADYSQIELVILAHLSEDESLIEAF
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCEEEEEEEEECCCCHHHHHHH
THKKDIHIQTASKLFKVDEANITPSMRRIAKSINFGIIYRMSAFRLSQELSIKREEAQNF
HCCCCEEEEHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHH
IDSYFNLYSKIKTFIQNQIDFVKQNGYSETLLKRRRYIREINSQNYLERTGAERMAINSI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHH
IQGSASDIMKIAMIKVYNEFKNNNLKSKILLQVHDEMLIESPEEECEKAQIIIKEMMENA
HCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
YPLKIPLKTNIETGKSWGDIHQ
CCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]