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The map label for this gene is fus-1 [H]

Identifier: 203284445

GI number: 203284445

Start: 563779

End: 565860

Strand: Reverse

Name: fus-1 [H]

Synonym: BDU_541

Alternate gene names: 203284445

Gene position: 565860-563779 (Counterclockwise)

Preceding gene: 203284452

Following gene: 203284444

Centisome position: 60.74

GC content: 33.0

Gene sequence:

>2082_bases
ATGGACTACAAAAAATTGCGCAACATAGGTATTAGTGCCCATATCGACTCAGGGAAAACCACACTTACAGAACGTATTCT
ATTTTATTGTAACAAAATTCACGCTATTCATGAAGTTAAGGGAAAAGATGGTGTTGGAGCAACAATGGATTCAATGGAAC
TTGAAAAAGAACGAGGTATCACAATTGCATCAGCCGCAACTCATGTTGAATGGAAAAATCATCCAATCAATATTATTGAT
ACTCCGGGTCACGTTGATTTCACAATTGAAGTTGAACGTTCACTTAGAGTTTTAGATGGAGCAATACTTATTCTTGATTC
CGTTGCAGGTGTCCAATCTCAATCAATTACAGTTGACAGACAATTAAAAAGATATAATGTACCACGACTTGCTTTTGTGA
ATAAATGTGATAAGACCGGAGCAAATCCTAGTAACGTAAAAGATCAACTTAAAGATAAACTTGGCTTAAATTCAGTTTTA
ATGCAAATTCCAATTGGACTTGAAGATAAACACATGGGAGTTGTTGATCTTGTTGTTATGAAAGCCTATTACTTTGAAGG
TAAAGATGGAACAGAAATTATAGAAAAAGAAATCCCTGCTGAATTGTTAAATGAGGCTGAAGAAAAGCGAAAAATAATGC
TTGATGCCTTATCTGATTTTAATGATGAACTTATGGAACTACATATGGAAGGAAAAAATGTGGCTCTTGAGACAATATAC
GACGCAATTAGGACAGGAACCTTATCTTTAAAACTTTGTCCAGTATTTATGGGTTCTGCATATAAAAACAAAGGAGTACA
ACTTTTACTTGATGCTGTAAATAAATTTTTACCTTCACCTCATGATATTCAAAACATAGCACTTGACTTAAATGCAAATG
AAAAAGAAATTAAACTTAAAACTGACGAAACATTACCAACTGTAGCATTAGCTTTCAAACTAGAAGACGGACAATACGGA
CAATTAACTTATGTCAGGATATATCAAGGAGTTTTAAAAAAAGGACAAGAATTAATAAATTCAAGAACTTCTAAAAAGTT
TAAAGTTGGAAGACTCATTAGAATGCATGCCAATAATACTGAAGACATTGAATTTGGAAGCAGTGGCGATATTGTAGCTT
TATTTGGAATAGAATGCGCATCAGGAGATACATTCTGTGACCCTTCTATTAATTACTCAATGACATCAATGTATATCCCA
GAACCAGTAATATCTCTATCAATCAAACCCAAAGATAAAAAATCAGCTGACAATATGGCAAAAGCTCTTGGACGATTTAC
TAAAGAAGATCCTACATTTAAAACTTATGTAGATAATGAATCAAAAGAAACAATAATACAAGGAATGGGTGAATTACACT
TAGAAGTTTATATTGAGAGAATGAAACGAGAATTTAAAGCCGAAGTTGAAACAGGAATGCCCCAAGTAGCATATAGAGAA
ACCATTACAGAAAAAGCAGAATTTAATTATACTCATAAAAAACAATCAGGTGGTGCAGGTCAATTTGGAAGAGTTGCCGG
ATTTATGGAACCACTTGATACAGAAGAACAAACTTACGAATTTGTCAATCTTATAAAAGGTGGAGTAATTCCAACAGAAT
ATATTCCATCATGTGATAAAGGATTTCAAAAGGCTATGGAAAAGGGAACCTTAATTGGATTCCCAATCGTTGGAGTCAAA
ATTACAATTAATGATGGACAATACCATATTGTTGATTCATCAGATATTGCATTTCAATTAGCAGCAATTGGAGCATTTAG
AGAAGCTTATAATAAGGCAAAACCTACAATACTAGAACCAATTATGAAAGTAACTCTTGAAGGACCAACAGAATTCCAAG
GAAATATGTTTGGCCTACTCAATCAAAGAAGAGGAATAATTTTAGGTTCTTCTGAAGAAGGCACTTTCTCAAAGGTCGAA
GCCGAAGTACCGCTAAGTGAAATGTTTGGATTTTCAACTGTTTTAAGATCATCTACACAAGGAAAAGCTGAATTTTCAAT
GGAATTTTTAAGATATGGTAAAGTTCCAAATGCAACATTTAATGAACTATGTAAAAAATTTAACGAACAAAACAATAAAT
AA

Upstream 100 bases:

>100_bases
CAACTTGAAGAGTTTAAAATAGACTTTTCTAAAAAAATCATGTAAAATACTAAAGACAATTAATGTTCTAAGTAATACAC
GCTCAGGAGGAAAATATTCC

Downstream 100 bases:

>100_bases
GGAGGAATAATAATGCTTGAATTAACGCAAGATAAACAAGAAATAAAAATAAAAAATAAATTTTTGGCTCATGTTTTTGG
ATTAATGTCAGTTGGGCTTT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 1 [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MDYKKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELEKERGITIASAATHVEWKNHPINIID
TPGHVDFTIEVERSLRVLDGAILILDSVAGVQSQSITVDRQLKRYNVPRLAFVNKCDKTGANPSNVKDQLKDKLGLNSVL
MQIPIGLEDKHMGVVDLVVMKAYYFEGKDGTEIIEKEIPAELLNEAEEKRKIMLDALSDFNDELMELHMEGKNVALETIY
DAIRTGTLSLKLCPVFMGSAYKNKGVQLLLDAVNKFLPSPHDIQNIALDLNANEKEIKLKTDETLPTVALAFKLEDGQYG
QLTYVRIYQGVLKKGQELINSRTSKKFKVGRLIRMHANNTEDIEFGSSGDIVALFGIECASGDTFCDPSINYSMTSMYIP
EPVISLSIKPKDKKSADNMAKALGRFTKEDPTFKTYVDNESKETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
TITEKAEFNYTHKKQSGGAGQFGRVAGFMEPLDTEEQTYEFVNLIKGGVIPTEYIPSCDKGFQKAMEKGTLIGFPIVGVK
ITINDGQYHIVDSSDIAFQLAAIGAFREAYNKAKPTILEPIMKVTLEGPTEFQGNMFGLLNQRRGIILGSSEEGTFSKVE
AEVPLSEMFGFSTVLRSSTQGKAEFSMEFLRYGKVPNATFNELCKKFNEQNNK

Sequences:

>Translated_693_residues
MDYKKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELEKERGITIASAATHVEWKNHPINIID
TPGHVDFTIEVERSLRVLDGAILILDSVAGVQSQSITVDRQLKRYNVPRLAFVNKCDKTGANPSNVKDQLKDKLGLNSVL
MQIPIGLEDKHMGVVDLVVMKAYYFEGKDGTEIIEKEIPAELLNEAEEKRKIMLDALSDFNDELMELHMEGKNVALETIY
DAIRTGTLSLKLCPVFMGSAYKNKGVQLLLDAVNKFLPSPHDIQNIALDLNANEKEIKLKTDETLPTVALAFKLEDGQYG
QLTYVRIYQGVLKKGQELINSRTSKKFKVGRLIRMHANNTEDIEFGSSGDIVALFGIECASGDTFCDPSINYSMTSMYIP
EPVISLSIKPKDKKSADNMAKALGRFTKEDPTFKTYVDNESKETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
TITEKAEFNYTHKKQSGGAGQFGRVAGFMEPLDTEEQTYEFVNLIKGGVIPTEYIPSCDKGFQKAMEKGTLIGFPIVGVK
ITINDGQYHIVDSSDIAFQLAAIGAFREAYNKAKPTILEPIMKVTLEGPTEFQGNMFGLLNQRRGIILGSSEEGTFSKVE
AEVPLSEMFGFSTVLRSSTQGKAEFSMEFLRYGKVPNATFNELCKKFNEQNNK
>Mature_693_residues
MDYKKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELEKERGITIASAATHVEWKNHPINIID
TPGHVDFTIEVERSLRVLDGAILILDSVAGVQSQSITVDRQLKRYNVPRLAFVNKCDKTGANPSNVKDQLKDKLGLNSVL
MQIPIGLEDKHMGVVDLVVMKAYYFEGKDGTEIIEKEIPAELLNEAEEKRKIMLDALSDFNDELMELHMEGKNVALETIY
DAIRTGTLSLKLCPVFMGSAYKNKGVQLLLDAVNKFLPSPHDIQNIALDLNANEKEIKLKTDETLPTVALAFKLEDGQYG
QLTYVRIYQGVLKKGQELINSRTSKKFKVGRLIRMHANNTEDIEFGSSGDIVALFGIECASGDTFCDPSINYSMTSMYIP
EPVISLSIKPKDKKSADNMAKALGRFTKEDPTFKTYVDNESKETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
TITEKAEFNYTHKKQSGGAGQFGRVAGFMEPLDTEEQTYEFVNLIKGGVIPTEYIPSCDKGFQKAMEKGTLIGFPIVGVK
ITINDGQYHIVDSSDIAFQLAAIGAFREAYNKAKPTILEPIMKVTLEGPTEFQGNMFGLLNQRRGIILGSSEEGTFSKVE
AEVPLSEMFGFSTVLRSSTQGKAEFSMEFLRYGKVPNATFNELCKKFNEQNNK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=696, Percent_Identity=52.1551724137931, Blast_Score=722, Evalue=0.0,
Organism=Homo sapiens, GI19923640, Length=710, Percent_Identity=34.9295774647887, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI25306287, Length=692, Percent_Identity=33.9595375722543, Blast_Score=325, Evalue=1e-88,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=40.7594936708861, Blast_Score=256, Evalue=7e-68,
Organism=Homo sapiens, GI157426893, Length=150, Percent_Identity=35.3333333333333, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=40.1459854014599, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI4503483, Length=156, Percent_Identity=34.6153846153846, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=41.5929203539823, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=41.5929203539823, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=41.5929203539823, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789738, Length=688, Percent_Identity=49.4186046511628, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=474, Percent_Identity=26.3713080168776, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=40.9395973154362, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788922, Length=160, Percent_Identity=35.625, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17533571, Length=677, Percent_Identity=49.3353028064993, Blast_Score=662, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17556745, Length=735, Percent_Identity=28.9795918367347, Blast_Score=271, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17557151, Length=153, Percent_Identity=37.9084967320261, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17506493, Length=168, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71988811, Length=178, Percent_Identity=33.1460674157303, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=190, Percent_Identity=32.1052631578947, Blast_Score=79, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=694, Percent_Identity=53.4582132564842, Blast_Score=758, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322359, Length=787, Percent_Identity=30.1143583227446, Blast_Score=310, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6324707, Length=410, Percent_Identity=24.1463414634146, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320593, Length=410, Percent_Identity=24.1463414634146, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=156, Percent_Identity=33.974358974359, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=151, Percent_Identity=34.4370860927152, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24582462, Length=693, Percent_Identity=51.6594516594517, Blast_Score=728, Evalue=0.0,
Organism=Drosophila melanogaster, GI221458488, Length=706, Percent_Identity=32.0113314447592, Blast_Score=321, Evalue=1e-87,
Organism=Drosophila melanogaster, GI78706572, Length=161, Percent_Identity=34.7826086956522, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24585711, Length=160, Percent_Identity=38.125, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585713, Length=160, Percent_Identity=38.125, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585709, Length=160, Percent_Identity=38.125, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77350; Mature: 77350

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELEKERGI
CCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCE
TIASAATHVEWKNHPINIIDTPGHVDFTIEVERSLRVLDGAILILDSVAGVQSQSITVDR
EEEEEEEEEEECCCCEEEEECCCCEEEEEEECCHHHHHCCCEEEEEHHCCCCCCCEEHHH
QLKRYNVPRLAFVNKCDKTGANPSNVKDQLKDKLGLNSVLMQIPIGLEDKHMGVVDLVVM
HHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHH
KAYYFEGKDGTEIIEKEIPAELLNEAEEKRKIMLDALSDFNDELMELHMEGKNVALETIY
HHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHH
DAIRTGTLSLKLCPVFMGSAYKNKGVQLLLDAVNKFLPSPHDIQNIALDLNANEKEIKLK
HHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEE
TDETLPTVALAFKLEDGQYGQLTYVRIYQGVLKKGQELINSRTSKKFKVGRLIRMHANNT
CCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCC
EDIEFGSSGDIVALFGIECASGDTFCDPSINYSMTSMYIPEPVISLSIKPKDKKSADNMA
CCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHH
KALGRFTKEDPTFKTYVDNESKETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TITEKAEFNYTHKKQSGGAGQFGRVAGFMEPLDTEEQTYEFVNLIKGGVIPTEYIPSCDK
HHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHH
GFQKAMEKGTLIGFPIVGVKITINDGQYHIVDSSDIAFQLAAIGAFREAYNKAKPTILEP
HHHHHHHCCCEEEEEEEEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHH
IMKVTLEGPTEFQGNMFGLLNQRRGIILGSSEEGTFSKVEAEVPLSEMFGFSTVLRSSTQ
HHHEEECCCCCCCCCHHHHHCCCCCEEEECCCCCCHHEEEECCCHHHHHHHHHHHHCCCC
GKAEFSMEFLRYGKVPNATFNELCKKFNEQNNK
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDYKKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELEKERGI
CCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCE
TIASAATHVEWKNHPINIIDTPGHVDFTIEVERSLRVLDGAILILDSVAGVQSQSITVDR
EEEEEEEEEEECCCCEEEEECCCCEEEEEEECCHHHHHCCCEEEEEHHCCCCCCCEEHHH
QLKRYNVPRLAFVNKCDKTGANPSNVKDQLKDKLGLNSVLMQIPIGLEDKHMGVVDLVVM
HHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHH
KAYYFEGKDGTEIIEKEIPAELLNEAEEKRKIMLDALSDFNDELMELHMEGKNVALETIY
HHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHH
DAIRTGTLSLKLCPVFMGSAYKNKGVQLLLDAVNKFLPSPHDIQNIALDLNANEKEIKLK
HHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEE
TDETLPTVALAFKLEDGQYGQLTYVRIYQGVLKKGQELINSRTSKKFKVGRLIRMHANNT
CCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCC
EDIEFGSSGDIVALFGIECASGDTFCDPSINYSMTSMYIPEPVISLSIKPKDKKSADNMA
CCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHH
KALGRFTKEDPTFKTYVDNESKETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TITEKAEFNYTHKKQSGGAGQFGRVAGFMEPLDTEEQTYEFVNLIKGGVIPTEYIPSCDK
HHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHH
GFQKAMEKGTLIGFPIVGVKITINDGQYHIVDSSDIAFQLAAIGAFREAYNKAKPTILEP
HHHHHHHCCCEEEEEEEEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHH
IMKVTLEGPTEFQGNMFGLLNQRRGIILGSSEEGTFSKVEAEVPLSEMFGFSTVLRSSTQ
HHHEEECCCCCCCCCHHHHHCCCCCEEEECCCCCCHHEEEECCCHHHHHHHHHHHHCCCC
GKAEFSMEFLRYGKVPNATFNELCKKFNEQNNK
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA