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The map label for this gene is fliM [H]

Identifier: 203284199

GI number: 203284199

Start: 304188

End: 305246

Strand: Reverse

Name: fliM [H]

Synonym: BDU_281

Alternate gene names: 203284199

Gene position: 305246-304188 (Counterclockwise)

Preceding gene: 203284200

Following gene: 203284198

Centisome position: 32.76

GC content: 32.48

Gene sequence:

>1059_bases
ATGGCAGGTAATCCGGGAGCATTATCACAAGATGATATAGATAGTCTTTTAGAATCTATTAATTCGTCTGATAATTTATC
ATCAGATGATTCACTTTCTAATATTATATCTAGCCCTATGGGCAAGAAACAAAAAGTTAAAGTGTATGATTTTAAAAGAC
CAGATAAATTTTCAAAAGAACAAGTAAGAACAGTATCAAGCTTTCATGAAGCATTTGCAAGATATACTACAACTTCACTC
TCAGCTCTTTTGAGAAAAATGGTTCATGTACATGTAGCTTCAGTTGATCAGTTGACTTATGAAGAATTTATTCGATCTAT
TCCAAATCCTACTACCTTAGCAATAATTAATATGGATCCTCTTAAAGGTTCTGCTATATTTGAAGTTGATCCAACTATTG
CATTTGCAATAGTTGATAGGCTTTTTGGTGGTGATGGTGACACCATTAAAGATAAGAGTAGAGATTTAACAGAAATAGAA
CAGTCTGTAATGGAAAGTGTTATTATTCGTATTCTTGCTAATATGAGAGAGGCTTGGTCTCAAGTAGTTGATTTAAGGCC
TAGGTTTGGGCACATAGAAGTGAATCCTCAATTTGCTCAGATAGTTCCTCCAACAGAAATGGTTATTTTGGTTACACTTG
AAGTTAAGATAGGTAAAGTCGAAGGACTTATGAATTTTTGTTTGCCTTATATTACAATAGAACCTATTGTATCTAAACTG
TCAACAAGATATTGGCATTCTTTAATTGGTGTGGGAACTACCAGTGAGAATCTTGATGTACTTAGAGAAAAACTTGAATA
TACAAATATGCCTTTGGTGGCTGAGATAGGAGAAGTGAAATTAAAGGTAAGAGAAATCTTGTCTTTAGAGAAAGGGGATG
TTATTAATCTTGAAAATTCTTCAATAGATCAAGATTTGATTTTGAAAGTAGGAACTAAAGAAAAATTTAAATGTCGAATG
GGACTTGTTGGGAATAAAATTTCAGTTCAAATTACAAGAAAAGTTGGTGAGGTAGAGGATTTTGATTTGTTGAAAGAACT
TACGGAAGAAGTTGAATAA

Upstream 100 bases:

>100_bases
AAGGCCAGAATTAATAGTATTCTTAGAAATGGTGAAATAAAAGAGATAGCATTGACGCAAATTGATATTTTTGATATGTG
ATTTTTAAAAGGAATTTTAA

Downstream 100 bases:

>100_bases
CTTAAAGAGTTTTGATGTTTTAATGAATTTAATAGAGAGGTAAATAATGGATGTAGATGATAAGAATGATTTTAGTGAGG
AAAAACCTGAGATAAAGGGT

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MAGNPGALSQDDIDSLLESINSSDNLSSDDSLSNIISSPMGKKQKVKVYDFKRPDKFSKEQVRTVSSFHEAFARYTTTSL
SALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIE
QSVMESVIIRILANMREAWSQVVDLRPRFGHIEVNPQFAQIVPPTEMVILVTLEVKIGKVEGLMNFCLPYITIEPIVSKL
STRYWHSLIGVGTTSENLDVLREKLEYTNMPLVAEIGEVKLKVREILSLEKGDVINLENSSIDQDLILKVGTKEKFKCRM
GLVGNKISVQITRKVGEVEDFDLLKELTEEVE

Sequences:

>Translated_352_residues
MAGNPGALSQDDIDSLLESINSSDNLSSDDSLSNIISSPMGKKQKVKVYDFKRPDKFSKEQVRTVSSFHEAFARYTTTSL
SALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIE
QSVMESVIIRILANMREAWSQVVDLRPRFGHIEVNPQFAQIVPPTEMVILVTLEVKIGKVEGLMNFCLPYITIEPIVSKL
STRYWHSLIGVGTTSENLDVLREKLEYTNMPLVAEIGEVKLKVREILSLEKGDVINLENSSIDQDLILKVGTKEKFKCRM
GLVGNKISVQITRKVGEVEDFDLLKELTEEVE
>Mature_351_residues
AGNPGALSQDDIDSLLESINSSDNLSSDDSLSNIISSPMGKKQKVKVYDFKRPDKFSKEQVRTVSSFHEAFARYTTTSLS
ALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQ
SVMESVIIRILANMREAWSQVVDLRPRFGHIEVNPQFAQIVPPTEMVILVTLEVKIGKVEGLMNFCLPYITIEPIVSKLS
TRYWHSLIGVGTTSENLDVLREKLEYTNMPLVAEIGEVKLKVREILSLEKGDVINLENSSIDQDLILKVGTKEKFKCRMG
LVGNKISVQITRKVGEVEDFDLLKELTEEVE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=294, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 39433; Mature: 39302

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNPGALSQDDIDSLLESINSSDNLSSDDSLSNIISSPMGKKQKVKVYDFKRPDKFSKE
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCHH
QVRTVSSFHEAFARYTTTSLSALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
LKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQSVMESVIIRILANMREAWS
CCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QVVDLRPRFGHIEVNPQFAQIVPPTEMVILVTLEVKIGKVEGLMNFCLPYITIEPIVSKL
HHHHCCCCCCEEEECCCCEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
STRYWHSLIGVGTTSENLDVLREKLEYTNMPLVAEIGEVKLKVREILSLEKGDVINLENS
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCC
SIDQDLILKVGTKEKFKCRMGLVGNKISVQITRKVGEVEDFDLLKELTEEVE
CCCHHHEEECCCCHHHEEHCCCCCCEEEEEEEHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGNPGALSQDDIDSLLESINSSDNLSSDDSLSNIISSPMGKKQKVKVYDFKRPDKFSKE
CCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCHH
QVRTVSSFHEAFARYTTTSLSALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
LKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQSVMESVIIRILANMREAWS
CCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QVVDLRPRFGHIEVNPQFAQIVPPTEMVILVTLEVKIGKVEGLMNFCLPYITIEPIVSKL
HHHHCCCCCCEEEECCCCEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
STRYWHSLIGVGTTSENLDVLREKLEYTNMPLVAEIGEVKLKVREILSLEKGDVINLENS
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCC
SIDQDLILKVGTKEKFKCRMGLVGNKISVQITRKVGEVEDFDLLKELTEEVE
CCCHHHEEECCCCHHHEEHCCCCCCEEEEEEEHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]