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The map label for this gene is rho [H]

Identifier: 203284149

GI number: 203284149

Start: 246478

End: 248022

Strand: Reverse

Name: rho [H]

Synonym: BDU_230

Alternate gene names: 203284149

Gene position: 248022-246478 (Counterclockwise)

Preceding gene: 203284150

Following gene: 203284148

Centisome position: 26.62

GC content: 30.16

Gene sequence:

>1545_bases
ATGGATAGAAAATGTGAGGAATTTGATTTAGAGGATGAAATGAAGCGTTTGAATTCTTCTAAAGAATTAAAAATTGAAGA
TAATAATAAGAAAAAAGTAGTTAAAGTTGTTACTAAAAAAGAATCTGTTTCTAGTGCTTCTAGGAATTCTAATATTGATA
AATTAAGAGAATCTAATGAGCTTTCTTCTGATTTTGATTATGATATATCAGATCCAGATTTGGAGAGTAGTATTAAGACT
CTTGAACAAAGCAATATTATTAATTTTTTAGGTGGTAAGGATTCTATAGTTATTGATACTCTTTATGATAAGCCAATCAC
TGAAATTAGAAAAGTTGTTGAAGGACTTGGGACTAATCATACTATTGCGGTAACTATGAAAAAGACAGAATTGATATTTT
TACTTGTTAAAATATTAACAGAGCATAATATTAATGTTTTATTTACCGGTGTTCTTGATGTTTTAAGTGATGGTTATGGT
TTTTTGCGTACAGCATCTAATTCTTATCTGTCTGGAGGTAATGATGTTTATGTTTCTCCGTCTCAGATTAGGCTTTTTAA
TTTAAGAACGGGTGATATTTTATATGGACAAATTAGATCTCCAAGAGAAGGTGAGAGATTTTTTGCAATGATTAAGATTA
AGAGTATTAATGATCAAGATCCTACATTTGCACAAAATAGAATACCTTTTGATAATTTAACACCTTTATATCCTAGTTCA
AAATTAGATCTTGAATATGAAAATTGTAATATTTCAACTAGACTTATTAATTTGTTTGCACCTATTGGAAAGGGTCAAAG
AGCTTTAATAGTCTCGCCTCCAAAAGCTGGTAAGACTACTTTACTTCAAAAAATAGCTAATGCAATTACTACTAATTATC
CAGATATTGTTTTAATGATTTTACTTATTGATGAGAGACCTGAAGAGGTGACTGATATGATTCGTGGTGTTAAGGGTGAA
GTAATAGCATCTAATTTTGATGAACAGGCTAGTAGGCATGTACAAGTTGCAGAGATGGTCATTGAAAAGGCAAAAAGATT
AGTTGAGAATAAAAAAGATGTTGTGATTCTTTTAGATTCAATTACAAGGCTTGCTAGAGCTTATAATCAAACTATGCCAA
CTTCTGGTAAGATACTCTCAGGGGGTGTTGATTCTAATGCTTTACATAAACCAAAAAGATTTTTTGGTTCTGCTAGAAAT
ATTGAGGAAGGAGGTAGTCTTACTATTATAGCTACAGCTTTAGTTGATACTGGGAGTAAGATGGATGAAGTTATATTTGA
AGAATTCAAGAGTACTGGTAATATGGAGCTAATTCTTGATAGAAGTTTGGCAGATAGAAGACTTTTTCCTGCTATTAATA
TTAAGAAATCGGGGACAAGGAAAGAAGAATTACTTTTAAGTGAAGAAGAACGTTCTAAAATTTTGCTTATTAGGAAAATT
TTAAGTGGTGTTGATGATTATGAAGGAGTTGAAGCTTTGGTTGAAAAGATGAAAAAGAGTAAAAACAATGAAATTTTTTT
AAAAACCATGAGTAATGGAGATTAA

Upstream 100 bases:

>100_bases
ATACTTTGCAGTGAATTAATGTTATGTTTTATCATTTAATGATTAATTATTATTTTAAATAGAAATACCCTTCTTAAAAA
TTTATCTCATAGAGGAGATT

Downstream 100 bases:

>100_bases
TGAAATTGACTTTGTTAATAATTTAGACTAAACTTTGATTAAATTAAGAGAGGCTTTATGAAAAAAGATATACATCCTGT
TAGTAACTTAGTAGTTTTTA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MDRKCEEFDLEDEMKRLNSSKELKIEDNNKKKVVKVVTKKESVSSASRNSNIDKLRESNELSSDFDYDISDPDLESSIKT
LEQSNIINFLGGKDSIVIDTLYDKPITEIRKVVEGLGTNHTIAVTMKKTELIFLLVKILTEHNINVLFTGVLDVLSDGYG
FLRTASNSYLSGGNDVYVSPSQIRLFNLRTGDILYGQIRSPREGERFFAMIKIKSINDQDPTFAQNRIPFDNLTPLYPSS
KLDLEYENCNISTRLINLFAPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYPDIVLMILLIDERPEEVTDMIRGVKGE
VIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLDSITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSARN
IEEGGSLTIIATALVDTGSKMDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRKI
LSGVDDYEGVEALVEKMKKSKNNEIFLKTMSNGD

Sequences:

>Translated_514_residues
MDRKCEEFDLEDEMKRLNSSKELKIEDNNKKKVVKVVTKKESVSSASRNSNIDKLRESNELSSDFDYDISDPDLESSIKT
LEQSNIINFLGGKDSIVIDTLYDKPITEIRKVVEGLGTNHTIAVTMKKTELIFLLVKILTEHNINVLFTGVLDVLSDGYG
FLRTASNSYLSGGNDVYVSPSQIRLFNLRTGDILYGQIRSPREGERFFAMIKIKSINDQDPTFAQNRIPFDNLTPLYPSS
KLDLEYENCNISTRLINLFAPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYPDIVLMILLIDERPEEVTDMIRGVKGE
VIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLDSITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSARN
IEEGGSLTIIATALVDTGSKMDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRKI
LSGVDDYEGVEALVEKMKKSKNNEIFLKTMSNGD
>Mature_514_residues
MDRKCEEFDLEDEMKRLNSSKELKIEDNNKKKVVKVVTKKESVSSASRNSNIDKLRESNELSSDFDYDISDPDLESSIKT
LEQSNIINFLGGKDSIVIDTLYDKPITEIRKVVEGLGTNHTIAVTMKKTELIFLLVKILTEHNINVLFTGVLDVLSDGYG
FLRTASNSYLSGGNDVYVSPSQIRLFNLRTGDILYGQIRSPREGERFFAMIKIKSINDQDPTFAQNRIPFDNLTPLYPSS
KLDLEYENCNISTRLINLFAPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYPDIVLMILLIDERPEEVTDMIRGVKGE
VIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLDSITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSARN
IEEGGSLTIIATALVDTGSKMDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRKI
LSGVDDYEGVEALVEKMKKSKNNEIFLKTMSNGD

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=413, Percent_Identity=58.8377723970944, Blast_Score=504, Evalue=1e-144,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 57672; Mature: 57672

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKCEEFDLEDEMKRLNSSKELKIEDNNKKKVVKVVTKKESVSSASRNSNIDKLRESNE
CCCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHCC
LSSDFDYDISDPDLESSIKTLEQSNIINFLGGKDSIVIDTLYDKPITEIRKVVEGLGTNH
CCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCC
TIAVTMKKTELIFLLVKILTEHNINVLFTGVLDVLSDGYGFLRTASNSYLSGGNDVYVSP
EEEEEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCEEEECCCCCCCCCCEEEECC
SQIRLFNLRTGDILYGQIRSPREGERFFAMIKIKSINDQDPTFAQNRIPFDNLTPLYPSS
CEEEEEEECCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
KLDLEYENCNISTRLINLFAPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYPDIVLMI
CCEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHEEEE
LLIDERPEEVTDMIRGVKGEVIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLDS
EHCCCCCHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHH
ITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSARNIEEGGSLTIIATALVDTGSK
HHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEEECCCCCH
MDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRKI
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHH
LSGVDDYEGVEALVEKMKKSKNNEIFLKTMSNGD
HCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure
MDRKCEEFDLEDEMKRLNSSKELKIEDNNKKKVVKVVTKKESVSSASRNSNIDKLRESNE
CCCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHCC
LSSDFDYDISDPDLESSIKTLEQSNIINFLGGKDSIVIDTLYDKPITEIRKVVEGLGTNH
CCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCC
TIAVTMKKTELIFLLVKILTEHNINVLFTGVLDVLSDGYGFLRTASNSYLSGGNDVYVSP
EEEEEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCEEEECCCCCCCCCCEEEECC
SQIRLFNLRTGDILYGQIRSPREGERFFAMIKIKSINDQDPTFAQNRIPFDNLTPLYPSS
CEEEEEEECCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
KLDLEYENCNISTRLINLFAPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYPDIVLMI
CCEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHEEEE
LLIDERPEEVTDMIRGVKGEVIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLDS
EHCCCCCHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHH
ITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSARNIEEGGSLTIIATALVDTGSK
HHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEEECCCCCH
MDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRKI
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHH
LSGVDDYEGVEALVEKMKKSKNNEIFLKTMSNGD
HCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8451174; 9403685; 7812434 [H]