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The map label for this gene is trmE

Identifier: 203284100

GI number: 203284100

Start: 191484

End: 192878

Strand: Reverse

Name: trmE

Synonym: BDU_178

Alternate gene names: 203284100

Gene position: 192878-191484 (Counterclockwise)

Preceding gene: 203284106

Following gene: 203284099

Centisome position: 20.7

GC content: 31.04

Gene sequence:

>1395_bases
ATGAGCAGGCTTTTTCAGAAAGAAGATGATATTGTTGCACTTGCTACACCTTTTTTTAGTAGTGCTTTATGTGTAATTCG
TAGTAGTGGAGTTTCTGCAATTGAAAAATTTTCTAAAATGTTCTCAGATCCGAGTAAATTGCTTGAGGCATCTGGGCACA
CTATTCATTATGGATATATTCTAGATAACGAAACTAAAGAAAAATTGGATGAAGTTGTTGTTTGTCTTTATAGAGCGCCA
AAAAGTTTTACAGGTCAGGATTCAATAGAAGTGATGGCTCATGGTTCTCTTATTGGTATAAGGAGAATTATAGACTTTTT
TTTGAAAGTAGGTTTTCGAATGGCTGAGCCTGGTGAATTTACCCTTCGTTCTTTCTTGGCAGGGAAACTTGATCTTACTA
AAGCAGAGGCTATAAATGAATTAATTTCTGCTAAGACTAGGCAAGTACATGCTCTTGCTGTTAATAAACTTTCAGGTTCT
TTGTTTGATAAGATAGATTTGATTAAAAAAGATATTTTAAATTTTCTTTCAGCCATTAGTGTTTATCTTGATTATGAGAC
AGATGATAATGAAGTTGTTATTCCTGTTGATATTATTTTAAAAAGTAAATCTGAACTTGAAAGGCTAATTGATTCTTATG
ATACTGCAAGAAAATTGGAGAATGGTGTTACATTAGTGTTGGCTGGTTCTGTTAATGTTGGTAAATCTTCTCTTTTTAAT
TTGTTATTAAAAGAAGATAGGGCTATCGTGTCTTCTTATGCTGGTACTACACGAGATTATATTCAAGCAAGTTTTGAATT
TGATGGTATTTTGTTTAACGTATTTGATACAGCAGGGCTTAGAGAAACTAGTGATTTTGTTGAACAGTTGGGTATAGTGA
GAAGTAATTCTTTGATTAAGGAAGCATCTTTGATTTTTTATGTTGTTGATTTAAGTGGCAAATTAACGGATGATGATTTG
AAGTTTATTGATGCTTATAAGGAAGATTCTAGAGTGCTTTTTGTTTTAAATAAAGTGGATTTAGAGCAAAATAATCAAAC
AGTTGAATTTTTTAATTCTAATAATGTAGTTTCATTAAACACAGTAAAGATTAGTACTAAAACTTTATTTGGTATAAATT
CCCTTTATGATAGGATAAGGTCATTGATAGCTGTTGATTATATGAAGACTAGTGATTATGATGTTGTAATATCATCAACT
CGTCAGGCAGAACTTTTAAAGAGGGCTTATGCTTTAATTATTGAGTTGTTAAGCAAAATAGAACAAAATATAAGTTATGA
TATGTTGGCATTTGATGTTTACGAAGTGGTTAATGTTTTAGGGGAAATAACGGGTGAGGTTACTAGTGATGATGTGCTTG
ACAATATGTTTAAGAATTTTTGTTTAGGAAAATAG

Upstream 100 bases:

>100_bases
ACAAATTTGCAATATTGTTGTTTTTATTATTTTGTACTTTTGTTTTTTATTTTATTTTTTGATTTTTGTTATTTATGCTA
TGTTATAATTTCTATAATCT

Downstream 100 bases:

>100_bases
GTATGGATTTTGATGCTATTGTTATTGGAGGAGGGCATGCTGGTATTGAAGCATCTCTGGCTCTTGCAAGATTAAATTTT
AAAACAATTTTGATTACTCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSRLFQKEDDIVALATPFFSSALCVIRSSGVSAIEKFSKMFSDPSKLLEASGHTIHYGYILDNETKEKLDEVVVCLYRAP
KSFTGQDSIEVMAHGSLIGIRRIIDFFLKVGFRMAEPGEFTLRSFLAGKLDLTKAEAINELISAKTRQVHALAVNKLSGS
LFDKIDLIKKDILNFLSAISVYLDYETDDNEVVIPVDIILKSKSELERLIDSYDTARKLENGVTLVLAGSVNVGKSSLFN
LLLKEDRAIVSSYAGTTRDYIQASFEFDGILFNVFDTAGLRETSDFVEQLGIVRSNSLIKEASLIFYVVDLSGKLTDDDL
KFIDAYKEDSRVLFVLNKVDLEQNNQTVEFFNSNNVVSLNTVKISTKTLFGINSLYDRIRSLIAVDYMKTSDYDVVISST
RQAELLKRAYALIIELLSKIEQNISYDMLAFDVYEVVNVLGEITGEVTSDDVLDNMFKNFCLGK

Sequences:

>Translated_464_residues
MSRLFQKEDDIVALATPFFSSALCVIRSSGVSAIEKFSKMFSDPSKLLEASGHTIHYGYILDNETKEKLDEVVVCLYRAP
KSFTGQDSIEVMAHGSLIGIRRIIDFFLKVGFRMAEPGEFTLRSFLAGKLDLTKAEAINELISAKTRQVHALAVNKLSGS
LFDKIDLIKKDILNFLSAISVYLDYETDDNEVVIPVDIILKSKSELERLIDSYDTARKLENGVTLVLAGSVNVGKSSLFN
LLLKEDRAIVSSYAGTTRDYIQASFEFDGILFNVFDTAGLRETSDFVEQLGIVRSNSLIKEASLIFYVVDLSGKLTDDDL
KFIDAYKEDSRVLFVLNKVDLEQNNQTVEFFNSNNVVSLNTVKISTKTLFGINSLYDRIRSLIAVDYMKTSDYDVVISST
RQAELLKRAYALIIELLSKIEQNISYDMLAFDVYEVVNVLGEITGEVTSDDVLDNMFKNFCLGK
>Mature_463_residues
SRLFQKEDDIVALATPFFSSALCVIRSSGVSAIEKFSKMFSDPSKLLEASGHTIHYGYILDNETKEKLDEVVVCLYRAPK
SFTGQDSIEVMAHGSLIGIRRIIDFFLKVGFRMAEPGEFTLRSFLAGKLDLTKAEAINELISAKTRQVHALAVNKLSGSL
FDKIDLIKKDILNFLSAISVYLDYETDDNEVVIPVDIILKSKSELERLIDSYDTARKLENGVTLVLAGSVNVGKSSLFNL
LLKEDRAIVSSYAGTTRDYIQASFEFDGILFNVFDTAGLRETSDFVEQLGIVRSNSLIKEASLIFYVVDLSGKLTDDDLK
FIDAYKEDSRVLFVLNKVDLEQNNQTVEFFNSNNVVSLNTVKISTKTLFGINSLYDRIRSLIAVDYMKTSDYDVVISSTR
QAELLKRAYALIIELLSKIEQNISYDMLAFDVYEVVNVLGEITGEVTSDDVLDNMFKNFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=28.2978723404255, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=28.4782608695652, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=26.8924302788845, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI2367268, Length=484, Percent_Identity=30.9917355371901, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI87082120, Length=138, Percent_Identity=34.0579710144928, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=475, Percent_Identity=29.8947368421053, Blast_Score=161, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=28.6868686868687, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45550813, Length=476, Percent_Identity=30.0420168067227, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BORDL (B5RL05)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221840.1
- EnsemblBacteria:   EBBORT00000001276
- GeneID:   6918262
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_178
- GeneTree:   EBGT00050000007366
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51970; Mature: 51839

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 90-90 BINDING 129-129 BINDING 464-464

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLFQKEDDIVALATPFFSSALCVIRSSGVSAIEKFSKMFSDPSKLLEASGHTIHYGYI
CCCCCCCCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCEEEEEEE
LDNETKEKLDEVVVCLYRAPKSFTGQDSIEVMAHGSLIGIRRIIDFFLKVGFRMAEPGEF
ECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHH
TLRSFLAGKLDLTKAEAINELISAKTRQVHALAVNKLSGSLFDKIDLIKKDILNFLSAIS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHE
VYLDYETDDNEVVIPVDIILKSKSELERLIDSYDTARKLENGVTLVLAGSVNVGKSSLFN
EEEEEECCCCCEEEEHHEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
LLLKEDRAIVSSYAGTTRDYIQASFEFDGILFNVFDTAGLRETSDFVEQLGIVRSNSLIK
HHHHHHHHHHHHHCCCCHHHHEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHH
EASLIFYVVDLSGKLTDDDLKFIDAYKEDSRVLFVLNKVDLEQNNQTVEFFNSNNVVSLN
HCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEECCCCEEEEE
TVKISTKTLFGINSLYDRIRSLIAVDYMKTSDYDVVISSTRQAELLKRAYALIIELLSKI
EEEEEHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
EQNISYDMLAFDVYEVVNVLGEITGEVTSDDVLDNMFKNFCLGK
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRLFQKEDDIVALATPFFSSALCVIRSSGVSAIEKFSKMFSDPSKLLEASGHTIHYGYI
CCCCCCCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCEEEEEEE
LDNETKEKLDEVVVCLYRAPKSFTGQDSIEVMAHGSLIGIRRIIDFFLKVGFRMAEPGEF
ECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHH
TLRSFLAGKLDLTKAEAINELISAKTRQVHALAVNKLSGSLFDKIDLIKKDILNFLSAIS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHE
VYLDYETDDNEVVIPVDIILKSKSELERLIDSYDTARKLENGVTLVLAGSVNVGKSSLFN
EEEEEECCCCCEEEEHHEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
LLLKEDRAIVSSYAGTTRDYIQASFEFDGILFNVFDTAGLRETSDFVEQLGIVRSNSLIK
HHHHHHHHHHHHHCCCCHHHHEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHH
EASLIFYVVDLSGKLTDDDLKFIDAYKEDSRVLFVLNKVDLEQNNQTVEFFNSNNVVSLN
HCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEECCCCEEEEE
TVKISTKTLFGINSLYDRIRSLIAVDYMKTSDYDVVISSTRQAELLKRAYALIIELLSKI
EEEEEHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
EQNISYDMLAFDVYEVVNVLGEITGEVTSDDVLDNMFKNFCLGK
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA