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The map label for this gene is gidA [H]

Identifier: 203284099

GI number: 203284099

Start: 189619

End: 191499

Strand: Reverse

Name: gidA [H]

Synonym: BDU_177

Alternate gene names: 203284099

Gene position: 191499-189619 (Counterclockwise)

Preceding gene: 203284100

Following gene: 203284098

Centisome position: 20.55

GC content: 32.06

Gene sequence:

>1881_bases
TTGTTTAGGAAAATAGGTATGGATTTTGATGCTATTGTTATTGGAGGAGGGCATGCTGGTATTGAAGCATCTCTGGCTCT
TGCAAGATTAAATTTTAAAACAATTTTGATTACTCAAAATTTAGATACAGTAGGTAAACTATCTTGTAATCCTGCTATTG
GTGGGCTTGCTAAGGGAAATATGGTGAGGGAAATTGATGCTCTTGGTGGAGAGATGGGATGTTTAATTGATTTTAGTATG
ATTCAATTTCGTATTTTAAATAAAAGTAAGGGTCCTGCTGTACAAGCACCTCGTGCACAAGCTGATAAATTAGTATATCA
GATTAAAGCAAAGGAAACATTAGAGATGCAGAATAATCTTGAACTTTTTCAAGATACTGTTAGTGATTTTTTACTTAATC
CTATGAAAAATAAGATTGAAGGTGTCGTTACAGAAAGGGGTAATAAATTTACATCGCGTGTTGTAGTGCTTACAACGGGA
ACTTTTCTTAGAGGTAAAATATTCATAGGTGAATACAAGGCTTATATGGGAAGGATTTCTGAGCCAGCTGCTTTTGGTCT
TGAGAAAATTTTGCTTGAACTTGGATTTGAAATGGGTAGACTTAAGACAGGTACTCCTGCAAGGGTTCATAGGAGAAGTA
TTAATTTTGCAAAAACAGAAATTCAGTTTGGAGATTCTGATATCATTCCTTTTTCTTTTTCAAATACTAAAATAGATAAG
ACTCAACTTTCTTGTTATGTTACTTATACTAATAAAAAGACTCATGAGATAATTAGTGAAAATATGCATCTCTCTCCACT
TTATTCTGGGGAAATTGTTGGGAATGGTCCAAGATATTGTCCTTCTATTGAAGATAAAATTGTTAAATTTAAAGATAAAG
ATAAGCATCAAATTTTTATTGAGCCTGAGGGGATTAATACCCAAGAGATGTATCTTAATGGTTTAAGTTCATCTTTACCT
GAGGATGTTCAAAAGAGATTAATTAATAGCATTGATGGACTTGAGGATGCAGTAATTACAAGACCAGGATATGCTGTTGA
GTATGATTATATTAATCCCATTGAATTATATGCAAGCCTTGAGACTAAAAGGGTTGAAGGACTTTTTGTTGCAGGACAAA
CTAATGGATCATCTGGATATGAAGAAGCTGCAGCTCAAGGTTTGATGGCCGGAATTAATGCAGCACTTAAATTACAAAAA
AAAGATCCAATGATACTGCCAAGGACTAGTTCTTATATTGGTGTTTTAATTGACGATCTTGTTACTAAGGGTACAAAAGA
GCCTTATCGAATGTTTACTTCAAGAGCTGAACATAGACTTAATTTGAGGCATGATACTAGTGATAAAAGATTGATTAAAT
GGGGGTATGAAGTTGGATTGGTTAGTGAGGAGAAATATTCTAGGTATTTTTTTAAGGAAAAGCAAATTGAGGAAATTAAA
GAACTTTTGAGGCATAGACGTCTTAAAGAGAAAGAGGCTAGTAGTTTTGAATTAAAAAGGCATATTAATAAAGATTTTTA
TCATATTGTGAAAGATCCATATATTAATTTAAAGGATCTTGTTAATATTGATCCTAAGTTAAATGCTTCAAAAGTAATTC
TTGAACAAGTTGAATTAGACATTAAATATGAAGGCTACATTAATAGGCAAAAAGATTTGATTAGAAAAATGAATAATCTT
GAACTTATTAAATTGCCACTTGATTTTAATTATGAGATTGATGGTCTCTCTAGAGAGGCTAGAGAAAAATTTGTTAAAAT
TCAACCAGCTAATCTTGCTCAGGCAAGTAGGGTTTCTGGGGTTAGGAATACTGATATTCAAGTTTTGCTTATATATTTTT
CAAACCCTAAAAATAAAGTAGTACTTAATTTAGATTTATGA

Upstream 100 bases:

>100_bases
ATATGTTGGCATTTGATGTTTACGAAGTGGTTAATGTTTTAGGGGAAATAACGGGTGAGGTTACTAGTGATGATGTGCTT
GACAATATGTTTAAGAATTT

Downstream 100 bases:

>100_bases
TGACTAATTTTAAATTTGCTTTGAAAAATTTGAAAGTGAATTTTACTTCAGAAAATATTGATAAATTGAGATTTTATATA
GAGAAAGTTTTATTATTTAG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MFRKIGMDFDAIVIGGGHAGIEASLALARLNFKTILITQNLDTVGKLSCNPAIGGLAKGNMVREIDALGGEMGCLIDFSM
IQFRILNKSKGPAVQAPRAQADKLVYQIKAKETLEMQNNLELFQDTVSDFLLNPMKNKIEGVVTERGNKFTSRVVVLTTG
TFLRGKIFIGEYKAYMGRISEPAAFGLEKILLELGFEMGRLKTGTPARVHRRSINFAKTEIQFGDSDIIPFSFSNTKIDK
TQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDKHQIFIEPEGINTQEMYLNGLSSSLP
EDVQKRLINSIDGLEDAVITRPGYAVEYDYINPIELYASLETKRVEGLFVAGQTNGSSGYEEAAAQGLMAGINAALKLQK
KDPMILPRTSSYIGVLIDDLVTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKWGYEVGLVSEEKYSRYFFKEKQIEEIK
ELLRHRRLKEKEASSFELKRHINKDFYHIVKDPYINLKDLVNIDPKLNASKVILEQVELDIKYEGYINRQKDLIRKMNNL
ELIKLPLDFNYEIDGLSREAREKFVKIQPANLAQASRVSGVRNTDIQVLLIYFSNPKNKVVLNLDL

Sequences:

>Translated_626_residues
MFRKIGMDFDAIVIGGGHAGIEASLALARLNFKTILITQNLDTVGKLSCNPAIGGLAKGNMVREIDALGGEMGCLIDFSM
IQFRILNKSKGPAVQAPRAQADKLVYQIKAKETLEMQNNLELFQDTVSDFLLNPMKNKIEGVVTERGNKFTSRVVVLTTG
TFLRGKIFIGEYKAYMGRISEPAAFGLEKILLELGFEMGRLKTGTPARVHRRSINFAKTEIQFGDSDIIPFSFSNTKIDK
TQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDKHQIFIEPEGINTQEMYLNGLSSSLP
EDVQKRLINSIDGLEDAVITRPGYAVEYDYINPIELYASLETKRVEGLFVAGQTNGSSGYEEAAAQGLMAGINAALKLQK
KDPMILPRTSSYIGVLIDDLVTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKWGYEVGLVSEEKYSRYFFKEKQIEEIK
ELLRHRRLKEKEASSFELKRHINKDFYHIVKDPYINLKDLVNIDPKLNASKVILEQVELDIKYEGYINRQKDLIRKMNNL
ELIKLPLDFNYEIDGLSREAREKFVKIQPANLAQASRVSGVRNTDIQVLLIYFSNPKNKVVLNLDL
>Mature_626_residues
MFRKIGMDFDAIVIGGGHAGIEASLALARLNFKTILITQNLDTVGKLSCNPAIGGLAKGNMVREIDALGGEMGCLIDFSM
IQFRILNKSKGPAVQAPRAQADKLVYQIKAKETLEMQNNLELFQDTVSDFLLNPMKNKIEGVVTERGNKFTSRVVVLTTG
TFLRGKIFIGEYKAYMGRISEPAAFGLEKILLELGFEMGRLKTGTPARVHRRSINFAKTEIQFGDSDIIPFSFSNTKIDK
TQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDKHQIFIEPEGINTQEMYLNGLSSSLP
EDVQKRLINSIDGLEDAVITRPGYAVEYDYINPIELYASLETKRVEGLFVAGQTNGSSGYEEAAAQGLMAGINAALKLQK
KDPMILPRTSSYIGVLIDDLVTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKWGYEVGLVSEEKYSRYFFKEKQIEEIK
ELLRHRRLKEKEASSFELKRHINKDFYHIVKDPYINLKDLVNIDPKLNASKVILEQVELDIKYEGYINRQKDLIRKMNNL
ELIKLPLDFNYEIDGLSREAREKFVKIQPANLAQASRVSGVRNTDIQVLLIYFSNPKNKVVLNLDL

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=634, Percent_Identity=40.3785488958991, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI19882217, Length=659, Percent_Identity=38.8467374810319, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI183227703, Length=674, Percent_Identity=37.9821958456973, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI2367273, Length=615, Percent_Identity=47.3170731707317, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17534255, Length=630, Percent_Identity=39.0476190476191, Blast_Score=424, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321202, Length=612, Percent_Identity=41.5032679738562, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24658174, Length=625, Percent_Identity=42.4, Blast_Score=474, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70937; Mature: 70937

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKIGMDFDAIVIGGGHAGIEASLALARLNFKTILITQNLDTVGKLSCNPAIGGLAKGN
CCCCCCCCCCEEEEECCCCCCHHHEEEEEECEEEEEEECCCCCCCCEECCCCCCCCCCCC
MVREIDALGGEMGCLIDFSMIQFRILNKSKGPAVQAPRAQADKLVYQIKAKETLEMQNNL
HHHHHHHCCCCEEEEEEHHHHEEEEECCCCCCCCCCCCHHHHHEEEEEECHHHHHHHHHH
ELFQDTVSDFLLNPMKNKIEGVVTERGNKFTSRVVVLTTGTFLRGKIFIGEYKAYMGRIS
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEEEEEHHHHHHCCCC
EPAAFGLEKILLELGFEMGRLKTGTPARVHRRSINFAKTEIQFGDSDIIPFSFSNTKIDK
CCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHCCCEEEEEEEECCCCEEEEECCCCEEEC
TQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDKHQIFI
EEEEEEEEECCCHHHHHHHCCCEECCCCCCCEECCCCCCCCCHHHHHEEECCCCCCEEEE
EPEGINTQEMYLNGLSSSLPEDVQKRLINSIDGLEDAVITRPGYAVEYDYINPIELYASL
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEECCCCEEEECCCCHHHHHEEC
ETKRVEGLFVAGQTNGSSGYEEAAAQGLMAGINAALKLQKKDPMILPRTSSYIGVLIDDL
CCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCHHHHHHHHH
VTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKWGYEVGLVSEEKYSRYFFKEKQIEEIK
HHCCCCCHHHHHHHHHHHEEECCCCCCCCEEEEECCEEEEECHHHHHHHHHHHHHHHHHH
ELLRHRRLKEKEASSFELKRHINKDFYHIVKDPYINLKDLVNIDPKLNASKVILEQVELD
HHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHEEE
IKYEGYINRQKDLIRKMNNLELIKLPLDFNYEIDGLSREAREKFVKIQPANLAQASRVSG
EEECCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHHHHEEEECCCCCHHHHHHCC
VRNTDIQVLLIYFSNPKNKVVLNLDL
CCCCCEEEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MFRKIGMDFDAIVIGGGHAGIEASLALARLNFKTILITQNLDTVGKLSCNPAIGGLAKGN
CCCCCCCCCCEEEEECCCCCCHHHEEEEEECEEEEEEECCCCCCCCEECCCCCCCCCCCC
MVREIDALGGEMGCLIDFSMIQFRILNKSKGPAVQAPRAQADKLVYQIKAKETLEMQNNL
HHHHHHHCCCCEEEEEEHHHHEEEEECCCCCCCCCCCCHHHHHEEEEEECHHHHHHHHHH
ELFQDTVSDFLLNPMKNKIEGVVTERGNKFTSRVVVLTTGTFLRGKIFIGEYKAYMGRIS
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEEEEEHHHHHHCCCC
EPAAFGLEKILLELGFEMGRLKTGTPARVHRRSINFAKTEIQFGDSDIIPFSFSNTKIDK
CCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHCCCEEEEEEEECCCCEEEEECCCCEEEC
TQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDKHQIFI
EEEEEEEEECCCHHHHHHHCCCEECCCCCCCEECCCCCCCCCHHHHHEEECCCCCCEEEE
EPEGINTQEMYLNGLSSSLPEDVQKRLINSIDGLEDAVITRPGYAVEYDYINPIELYASL
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEECCCCEEEECCCCHHHHHEEC
ETKRVEGLFVAGQTNGSSGYEEAAAQGLMAGINAALKLQKKDPMILPRTSSYIGVLIDDL
CCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCHHHHHHHHH
VTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKWGYEVGLVSEEKYSRYFFKEKQIEEIK
HHCCCCCHHHHHHHHHHHEEECCCCCCCCEEEEECCEEEEECHHHHHHHHHHHHHHHHHH
ELLRHRRLKEKEASSFELKRHINKDFYHIVKDPYINLKDLVNIDPKLNASKVILEQVELD
HHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHEEE
IKYEGYINRQKDLIRKMNNLELIKLPLDFNYEIDGLSREAREKFVKIQPANLAQASRVSG
EEECCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHHHHEEEECCCCCHHHHHHCC
VRNTDIQVLLIYFSNPKNKVVLNLDL
CCCCCEEEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA