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The map label for this gene is hisS [H]

Identifier: 203284061

GI number: 203284061

Start: 144517

End: 145887

Strand: Reverse

Name: hisS [H]

Synonym: BDU_139

Alternate gene names: 203284061

Gene position: 145887-144517 (Counterclockwise)

Preceding gene: 203284063

Following gene: 203284059

Centisome position: 15.66

GC content: 28.23

Gene sequence:

>1371_bases
GTGGATGTTAGAACTTTAAAAGGTTTTAGAGATTATTTGCCGAAAGAAGCGTTAATTCGTACTCATATTATAAAACAAAT
ATATAGTGTTCTGGTTTCTTATAATTTTGATTTAATAGATACTCCTATTCTTGAGTATTCTGAATTTCTTTTAAAAAAGG
GTGGGGATGAAGTAGAAAAGCAAATTTATAGATTTAAGGATAATGGAGATAGGGATGTTTCCATGCGTTTTGACTTGACC
GTTCCTTTTGCAAGATTTATGGCTACTAATAAATCTAAGATTAAATTTCCTTTCAGAAGGTCTCAAATAGGTAAGGTATT
TAGGGGTGAGAATACTCAAAAGGGCAGATATAGAGAGTTCATGCAATTTGATTTTGATATATTAGGTGAGGATACTTTTC
GTAGTGATGCTGAGATTTTATCTATTGTTTATTGTGGACTTGAAGAGGTTTTTTTAAATTTTATAGAAGGTATTAATATA
AAATTTGTTATTCACTTTTCTCATATTGGAATATTAAATACTTATATTGAAAAGTTGGGATTAAAGGATAAATCCATTTT
TATTTTAAGGAATATAGACAAATTAGACAAAATAGGAATTGAGGCTGTACGAGAAAATTTGCTTTTAGAGTTAAGTGAAG
AGCATGTTGATTTAGTATTAGAATTTGTAAATTTAGATGGAACTTTTAGTAATAAAGTAAAGGCTTTAAGAAGTATTTTA
GGAGATAATGATGCTGTTAAGAGAATAGAAGATATTTTTGTACATTTGAATGCATTGGGAATTCAAGATGCTTTTAATTT
TAATTTAAAGATAGTGCGTGGACTTGATTATTATACCGGACTTGTATTTGAGGCTGAAATGTTAGGTGTTAATATGGGAA
GTATTTGTAGTGGTGGGAGATATGATAATTTATTGTCTTTATTTTCTGGTTCTTTGCAAAAAGTTTCAGGGGTTGGAGGA
TCTTTTGGTATTGATAGGATTCAAGATATAATTGAAATTGATAAATTTAATTACATTAAGTTGTTTGTGGTTAAAGCCAG
TTCTAGAGTATTAATTGTTAATATAGACGATACTTTGCAAGATTATTATTATAAACTTGCAGATAAATTTAGAAGACATG
ATTATTCTAAGATAAATAATATTGCTTGTGAGGTATATCCTAAGAGTAAAGATGGTAAAAATATTAAGATTCAAATAGAG
TATGCTCTTGCTAAGGCTATAAGATTTTTAATTTTTGTGGGTCAAGAAGAATATCAAGAAGGTAAGTTAAAGGTTAGAGA
TTTAACCAAAAAGGAAGAACTGTTATTGTCTTTTGATGAGGCTATTAAGTTTATTAAGGGTAATGATAAATTTTTATGCA
CACCTTTTTAA

Upstream 100 bases:

>100_bases
ACTTAAGAATTTTATTATTAATTTTGATTAATTTTTTCTCATATTTATAATATTATATTATGAAAAGATTAAATTTGAAA
TTAGTAGAGGTTGAAATATA

Downstream 100 bases:

>100_bases
TTAAAGGTGGTTAAATTTTATTACTTTTTTAAATTAAATTTATTCTTTTTCTTTTTTGATTTTTCGAATTGTGATTGCAT
TTAAGAGTAATGTATTTGTA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDVRTLKGFRDYLPKEALIRTHIIKQIYSVLVSYNFDLIDTPILEYSEFLLKKGGDEVEKQIYRFKDNGDRDVSMRFDLT
VPFARFMATNKSKIKFPFRRSQIGKVFRGENTQKGRYREFMQFDFDILGEDTFRSDAEILSIVYCGLEEVFLNFIEGINI
KFVIHFSHIGILNTYIEKLGLKDKSIFILRNIDKLDKIGIEAVRENLLLELSEEHVDLVLEFVNLDGTFSNKVKALRSIL
GDNDAVKRIEDIFVHLNALGIQDAFNFNLKIVRGLDYYTGLVFEAEMLGVNMGSICSGGRYDNLLSLFSGSLQKVSGVGG
SFGIDRIQDIIEIDKFNYIKLFVVKASSRVLIVNIDDTLQDYYYKLADKFRRHDYSKINNIACEVYPKSKDGKNIKIQIE
YALAKAIRFLIFVGQEEYQEGKLKVRDLTKKEELLLSFDEAIKFIKGNDKFLCTPF

Sequences:

>Translated_456_residues
MDVRTLKGFRDYLPKEALIRTHIIKQIYSVLVSYNFDLIDTPILEYSEFLLKKGGDEVEKQIYRFKDNGDRDVSMRFDLT
VPFARFMATNKSKIKFPFRRSQIGKVFRGENTQKGRYREFMQFDFDILGEDTFRSDAEILSIVYCGLEEVFLNFIEGINI
KFVIHFSHIGILNTYIEKLGLKDKSIFILRNIDKLDKIGIEAVRENLLLELSEEHVDLVLEFVNLDGTFSNKVKALRSIL
GDNDAVKRIEDIFVHLNALGIQDAFNFNLKIVRGLDYYTGLVFEAEMLGVNMGSICSGGRYDNLLSLFSGSLQKVSGVGG
SFGIDRIQDIIEIDKFNYIKLFVVKASSRVLIVNIDDTLQDYYYKLADKFRRHDYSKINNIACEVYPKSKDGKNIKIQIE
YALAKAIRFLIFVGQEEYQEGKLKVRDLTKKEELLLSFDEAIKFIKGNDKFLCTPF
>Mature_456_residues
MDVRTLKGFRDYLPKEALIRTHIIKQIYSVLVSYNFDLIDTPILEYSEFLLKKGGDEVEKQIYRFKDNGDRDVSMRFDLT
VPFARFMATNKSKIKFPFRRSQIGKVFRGENTQKGRYREFMQFDFDILGEDTFRSDAEILSIVYCGLEEVFLNFIEGINI
KFVIHFSHIGILNTYIEKLGLKDKSIFILRNIDKLDKIGIEAVRENLLLELSEEHVDLVLEFVNLDGTFSNKVKALRSIL
GDNDAVKRIEDIFVHLNALGIQDAFNFNLKIVRGLDYYTGLVFEAEMLGVNMGSICSGGRYDNLLSLFSGSLQKVSGVGG
SFGIDRIQDIIEIDKFNYIKLFVVKASSRVLIVNIDDTLQDYYYKLADKFRRHDYSKINNIACEVYPKSKDGKNIKIQIE
YALAKAIRFLIFVGQEEYQEGKLKVRDLTKKEELLLSFDEAIKFIKGNDKFLCTPF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=477, Percent_Identity=31.8658280922432, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI15029520, Length=456, Percent_Identity=30.2631578947368, Blast_Score=189, Evalue=5e-48,
Organism=Escherichia coli, GI1788861, Length=451, Percent_Identity=25.7206208425721, Blast_Score=118, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71993693, Length=478, Percent_Identity=30.7531380753138, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71993686, Length=478, Percent_Identity=30.7531380753138, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6325290, Length=473, Percent_Identity=32.3467230443975, Blast_Score=179, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24643061, Length=470, Percent_Identity=30.6382978723404, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24643059, Length=489, Percent_Identity=29.4478527607362, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 52697; Mature: 52697

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRTLKGFRDYLPKEALIRTHIIKQIYSVLVSYNFDLIDTPILEYSEFLLKKGGDEVEK
CCCCHHCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHH
QIYRFKDNGDRDVSMRFDLTVPFARFMATNKSKIKFPFRRSQIGKVFRGENTQKGRYREF
HHHHHCCCCCCEEEEEEEEECHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCHHHHHH
MQFDFDILGEDTFRSDAEILSIVYCGLEEVFLNFIEGINIKFVIHFSHIGILNTYIEKLG
HHCCHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHC
LKDKSIFILRNIDKLDKIGIEAVRENLLLELSEEHVDLVLEFVNLDGTFSNKVKALRSIL
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GDNDAVKRIEDIFVHLNALGIQDAFNFNLKIVRGLDYYTGLVFEAEMLGVNMGSICSGGR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHEEEHHHHCCCCCCCCCCCC
YDNLLSLFSGSLQKVSGVGGSFGIDRIQDIIEIDKFNYIKLFVVKASSRVLIVNIDDTLQ
HHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCHHHH
DYYYKLADKFRRHDYSKINNIACEVYPKSKDGKNIKIQIEYALAKAIRFLIFVGQEEYQE
HHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHEECCHHHCC
GKLKVRDLTKKEELLLSFDEAIKFIKGNDKFLCTPF
CCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEECCC
>Mature Secondary Structure
MDVRTLKGFRDYLPKEALIRTHIIKQIYSVLVSYNFDLIDTPILEYSEFLLKKGGDEVEK
CCCCHHCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHH
QIYRFKDNGDRDVSMRFDLTVPFARFMATNKSKIKFPFRRSQIGKVFRGENTQKGRYREF
HHHHHCCCCCCEEEEEEEEECHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCHHHHHH
MQFDFDILGEDTFRSDAEILSIVYCGLEEVFLNFIEGINIKFVIHFSHIGILNTYIEKLG
HHCCHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHC
LKDKSIFILRNIDKLDKIGIEAVRENLLLELSEEHVDLVLEFVNLDGTFSNKVKALRSIL
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GDNDAVKRIEDIFVHLNALGIQDAFNFNLKIVRGLDYYTGLVFEAEMLGVNMGSICSGGR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHEEEHHHHCCCCCCCCCCCC
YDNLLSLFSGSLQKVSGVGGSFGIDRIQDIIEIDKFNYIKLFVVKASSRVLIVNIDDTLQ
HHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCHHHH
DYYYKLADKFRRHDYSKINNIACEVYPKSKDGKNIKIQIEYALAKAIRFLIFVGQEEYQE
HHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHEECCHHHCC
GKLKVRDLTKKEELLLSFDEAIKFIKGNDKFLCTPF
CCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]