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The map label for this gene is ybjF [C]

Identifier: 197119265

GI number: 197119265

Start: 3336826

End: 3338544

Strand: Direct

Name: ybjF [C]

Synonym: Gbem_2893

Alternate gene names: 197119265

Gene position: 3336826-3338544 (Clockwise)

Preceding gene: 308535323

Following gene: 197119266

Centisome position: 72.3

GC content: 62.01

Gene sequence:

>1719_bases
ATGAAGTTTGAGCACTCCCGTCCCCAGTCACAAAAGCCATACAAGAAAGACGACTCAGCCAGAACCGAAGGCGCCGAGTC
CAGCGCTACCCCCAAGTACTCCAAAAACACCTCAACTACAGCGCGCAAACCCAAAGCCGCCCAGACTGAGCGCACCGAAA
GCACCCGCAGCTCCAGGGGCGAAACTCCCGCTGCAGCGTTTGGCTCACGTCCCGCCCGCCCGAAGGGAGATCGTTCGGCG
GCGCCTCCCGCACCGGGCAAAGGCAGGGACCGTAAACCGGCGGCAAAGACAGCCGGGAACAGACCGGAGAGGACGCCTGC
TGGAAAGGGAGGAGCATCTTCCCGCGACGAGCGCCAGCCTACGCTGCGCAGCGGACAGGTGCTGGAGCTGAACATTCTCG
CTACCAACGACGAGGGCTTCGGCGTGGCCAACCACGAGGGGACCCGCGTGCTGGTGGCAGGGGGACTTCCGGGCGAGGTG
CTGGTGGCGAAGATCACCTACGTCGGCCGCAGGGAAGCCTTCGCCCATACCATAAAGTGTCTGAAGCGTTCTCCGGACCG
CAACCCCTCTCCCGCCTGCACCCTGGGACGGGTGTGCGACGGCTGCGGACTGATGCAGATGCGCTACCCGGCCCAGTTGG
CATGGAAAAAGTCGCTGGTCGCCCGCCACTTCCGGAAATACCCCTCTCTTTTCGAGGTCGCCATCAGGGAAACCATCGGC
TCGCCCAAGGAGCTTGGATATCGCAACACGGCAAAACTTGTCATTGCCGGGAAGAACAACGATCCCGTCATCGGCATCTA
CCGTCGCAACACCCATGACGTCTTGCAGATCGAGGACTGCGCGCTGCACCACCCGCTGATCAATAAGGTGGTGAAGGCTG
CCAAGGCCGGGATCAAGAAAGGAAAGGTGCAGATCTACAACCCCAAGAGCGAGATGGGGCTTTTGCGCTACCTGGTGGTT
CGCGTGGCCGAAAAGACCAATCAGGTCATGGTGGTTCTGGTGACGACGGACCAGGGTTACAACGAGATGCACCACCTGGC
GAAGTTCATTCAGCAGGCGGTCCCGGAGGTCTCGGTGGTGGCGCAGAACATCAACACCTCGACCGGCAACGTGATCTTCG
GCACCAAGGACCGCTCCATTACCAAGGCCCAGACGCTGAAGGCGTTCGTGGGTGACAAGATCTTCAACCTTTCGCCCCAC
TCCTTCTTCCAGGTCAACAGCGGTGCCGCGCGCATCATCTATGAGAAGGTGCGGGAGCTGGCGAACCTGAAGGGGACCGA
GCGGGTCATCGACGTCTACTGCGGCATCGGCGGCATCTCGCTCTATCTCGCCGACCAGGCTCGCGAAGTGGTCGGGATCG
AATTCGTCGAGGCAGCGGTCGCCGACGCCACCATCAACGCGGCACTGAACCACGTGGAGAACTGTCACTTCGAGGCCGGC
GACGCCGTGCATCTGATTGACGAGATCGGGGAGGAAGGGGGCGCGGACCTGATCGTGCTCAACCCGCCGCGGAAAGGGTG
CGACGAGAAGGTGTTGAGGAGCGTGGCCGCCATCAATCCCCCACGCATCATCTACGTTTCCTGCTCGCCCGAAACGCTGG
CGCGGGATCTGGACATCCTCTCCGGGCTCGGCTACCGCACCGTGGAGGTGCAGCCGGTGGACATGTTCCCGCAGACGGTG
CATGTCGAGGACGTGGCGCTTCTGGAAAAAAAGCAGTGA

Upstream 100 bases:

>100_bases
TGACAGGAACTTCTTTTACCCTTATGTAAGAAGCATGAAAAATAAAAGCGAAGCACCGAAAAGAGATAGAGCGAAGCAGG
TTGCATCAGACGGCAAGTCG

Downstream 100 bases:

>100_bases
CAACAGCGGCAGTAATGCCGCTTCTAGGGCTGCCATGGATGCTTTGATTCTACGACTGAAGAAGTTCTTCCCTGCGTCCC
TGCACTCCCGTATCTTCCTC

Product: RNA methyltransferase, TrmA family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKFEHSRPQSQKPYKKDDSARTEGAESSATPKYSKNTSTTARKPKAAQTERTESTRSSRGETPAAAFGSRPARPKGDRSA
APPAPGKGRDRKPAAKTAGNRPERTPAGKGGASSRDERQPTLRSGQVLELNILATNDEGFGVANHEGTRVLVAGGLPGEV
LVAKITYVGRREAFAHTIKCLKRSPDRNPSPACTLGRVCDGCGLMQMRYPAQLAWKKSLVARHFRKYPSLFEVAIRETIG
SPKELGYRNTAKLVIAGKNNDPVIGIYRRNTHDVLQIEDCALHHPLINKVVKAAKAGIKKGKVQIYNPKSEMGLLRYLVV
RVAEKTNQVMVVLVTTDQGYNEMHHLAKFIQQAVPEVSVVAQNINTSTGNVIFGTKDRSITKAQTLKAFVGDKIFNLSPH
SFFQVNSGAARIIYEKVRELANLKGTERVIDVYCGIGGISLYLADQAREVVGIEFVEAAVADATINAALNHVENCHFEAG
DAVHLIDEIGEEGGADLIVLNPPRKGCDEKVLRSVAAINPPRIIYVSCSPETLARDLDILSGLGYRTVEVQPVDMFPQTV
HVEDVALLEKKQ

Sequences:

>Translated_572_residues
MKFEHSRPQSQKPYKKDDSARTEGAESSATPKYSKNTSTTARKPKAAQTERTESTRSSRGETPAAAFGSRPARPKGDRSA
APPAPGKGRDRKPAAKTAGNRPERTPAGKGGASSRDERQPTLRSGQVLELNILATNDEGFGVANHEGTRVLVAGGLPGEV
LVAKITYVGRREAFAHTIKCLKRSPDRNPSPACTLGRVCDGCGLMQMRYPAQLAWKKSLVARHFRKYPSLFEVAIRETIG
SPKELGYRNTAKLVIAGKNNDPVIGIYRRNTHDVLQIEDCALHHPLINKVVKAAKAGIKKGKVQIYNPKSEMGLLRYLVV
RVAEKTNQVMVVLVTTDQGYNEMHHLAKFIQQAVPEVSVVAQNINTSTGNVIFGTKDRSITKAQTLKAFVGDKIFNLSPH
SFFQVNSGAARIIYEKVRELANLKGTERVIDVYCGIGGISLYLADQAREVVGIEFVEAAVADATINAALNHVENCHFEAG
DAVHLIDEIGEEGGADLIVLNPPRKGCDEKVLRSVAAINPPRIIYVSCSPETLARDLDILSGLGYRTVEVQPVDMFPQTV
HVEDVALLEKKQ
>Mature_572_residues
MKFEHSRPQSQKPYKKDDSARTEGAESSATPKYSKNTSTTARKPKAAQTERTESTRSSRGETPAAAFGSRPARPKGDRSA
APPAPGKGRDRKPAAKTAGNRPERTPAGKGGASSRDERQPTLRSGQVLELNILATNDEGFGVANHEGTRVLVAGGLPGEV
LVAKITYVGRREAFAHTIKCLKRSPDRNPSPACTLGRVCDGCGLMQMRYPAQLAWKKSLVARHFRKYPSLFEVAIRETIG
SPKELGYRNTAKLVIAGKNNDPVIGIYRRNTHDVLQIEDCALHHPLINKVVKAAKAGIKKGKVQIYNPKSEMGLLRYLVV
RVAEKTNQVMVVLVTTDQGYNEMHHLAKFIQQAVPEVSVVAQNINTSTGNVIFGTKDRSITKAQTLKAFVGDKIFNLSPH
SFFQVNSGAARIIYEKVRELANLKGTERVIDVYCGIGGISLYLADQAREVVGIEFVEAAVADATINAALNHVENCHFEAG
DAVHLIDEIGEEGGADLIVLNPPRKGCDEKVLRSVAAINPPRIIYVSCSPETLARDLDILSGLGYRTVEVQPVDMFPQTV
HVEDVALLEKKQ

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=367, Percent_Identity=29.700272479564, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI51173878, Length=367, Percent_Identity=29.700272479564, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI269784680, Length=191, Percent_Identity=32.9842931937173, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI269784676, Length=191, Percent_Identity=32.9842931937173, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI20149688, Length=191, Percent_Identity=32.9842931937173, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI269784678, Length=191, Percent_Identity=32.9842931937173, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1787083, Length=378, Percent_Identity=27.2486772486773, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789148, Length=462, Percent_Identity=26.6233766233766, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1790403, Length=386, Percent_Identity=26.9430051813472, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71997438, Length=165, Percent_Identity=32.1212121212121, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=410, Percent_Identity=24.6341463414634, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24666579, Length=421, Percent_Identity=26.603325415677, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 62336; Mature: 62336

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEHSRPQSQKPYKKDDSARTEGAESSATPKYSKNTSTTARKPKAAQTERTESTRSSRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
ETPAAAFGSRPARPKGDRSAAPPAPGKGRDRKPAAKTAGNRPERTPAGKGGASSRDERQP
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
TLRSGQVLELNILATNDEGFGVANHEGTRVLVAGGLPGEVLVAKITYVGRREAFAHTIKC
CCCCCCEEEEEEEEECCCCCEECCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHH
LKRSPDRNPSPACTLGRVCDGCGLMQMRYPAQLAWKKSLVARHFRKYPSLFEVAIRETIG
HHCCCCCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCC
SPKELGYRNTAKLVIAGKNNDPVIGIYRRNTHDVLQIEDCALHHPLINKVVKAAKAGIKK
CHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCEEEEHHHHHCCHHHHHHHHHHHHCCCC
GKVQIYNPKSEMGLLRYLVVRVAEKTNQVMVVLVTTDQGYNEMHHLAKFIQQAVPEVSVV
CCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEH
AQNINTSTGNVIFGTKDRSITKAQTLKAFVGDKIFNLSPHSFFQVNSGAARIIYEKVREL
HHCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCEECCCCCCEEEECCCCHHHHHHHHHHH
ANLKGTERVIDVYCGIGGISLYLADQAREVVGIEFVEAAVADATINAALNHVENCHFEAG
HCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAVHLIDEIGEEGGADLIVLNPPRKGCDEKVLRSVAAINPPRIIYVSCSPETLARDLDIL
CCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
SGLGYRTVEVQPVDMFPQTVHVEDVALLEKKQ
HCCCCEEEEEEECCCCCCEEEHHHHHHHHCCC
>Mature Secondary Structure
MKFEHSRPQSQKPYKKDDSARTEGAESSATPKYSKNTSTTARKPKAAQTERTESTRSSRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
ETPAAAFGSRPARPKGDRSAAPPAPGKGRDRKPAAKTAGNRPERTPAGKGGASSRDERQP
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
TLRSGQVLELNILATNDEGFGVANHEGTRVLVAGGLPGEVLVAKITYVGRREAFAHTIKC
CCCCCCEEEEEEEEECCCCCEECCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHH
LKRSPDRNPSPACTLGRVCDGCGLMQMRYPAQLAWKKSLVARHFRKYPSLFEVAIRETIG
HHCCCCCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCC
SPKELGYRNTAKLVIAGKNNDPVIGIYRRNTHDVLQIEDCALHHPLINKVVKAAKAGIKK
CHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCEEEEHHHHHCCHHHHHHHHHHHHCCCC
GKVQIYNPKSEMGLLRYLVVRVAEKTNQVMVVLVTTDQGYNEMHHLAKFIQQAVPEVSVV
CCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEH
AQNINTSTGNVIFGTKDRSITKAQTLKAFVGDKIFNLSPHSFFQVNSGAARIIYEKVREL
HHCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCEECCCCCCEEEECCCCHHHHHHHHHHH
ANLKGTERVIDVYCGIGGISLYLADQAREVVGIEFVEAAVADATINAALNHVENCHFEAG
HCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAVHLIDEIGEEGGADLIVLNPPRKGCDEKVLRSVAAINPPRIIYVSCSPETLARDLDIL
CCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
SGLGYRTVEVQPVDMFPQTVHVEDVALLEKKQ
HCCCCEEEEEEECCCCCCEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA