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The map label for this gene is gltD [H]

Identifier: 197119240

GI number: 197119240

Start: 3302498

End: 3303979

Strand: Direct

Name: gltD [H]

Synonym: Gbem_2867

Alternate gene names: 197119240

Gene position: 3302498-3303979 (Clockwise)

Preceding gene: 197119239

Following gene: 197119242

Centisome position: 71.56

GC content: 65.38

Gene sequence:

>1482_bases
ATGGGAAAACCAACCGGATTCATGGAATATCTTCGCGAACTCCCGTCGGACCGCGAGCCGCTGGAGCGGCTCAAAGATTG
GGACGAGTTCCACCTGCACCTGCCGGAGGAGAAGCTCCGCACCCAGGGCGCCCGTTGCATGGACTGCGGCATCCCGTTCT
GCCACACCGGGAAGCTGGTGAGCGGCATGGCCTGCGGCTGCCCGGTCAACAACCTGATCCCCGAGTTCAACGACCTGGTC
TACCGCGGGCTTTGGAAGCAGGCGTACGACAGGCTCTCGCGGACCAACAACTTCCCCGAGTTCACCGGCCGGGTCTGCCC
CGCCCCGTGCGAGGGGTCGTGCACGCTGGGTGCCAGCGAGCCTGCGGTCACCATCAAGAACATAGAAGTGAGCATCATCG
AGCGCGCCTGGGACGAAGGGTGGGTCACCCCTACCCTGCCGCAGGTCCGCACCGGCAAGAAGGTGGCGGTCGTTGGCTCC
GGCCCCGCAGGGCTTTCCGCGGCCGAGCAGCTCAACAAGGCCGGGCACCAGGTGACCGTGTTCGAACGGGCGCCGCTTCC
GGGGGGGCTTTTGATGTACGGCATCCCCAACATGAAGCTGGACAAGCAAAACATCGTCATGCGCCGCATCCGGCTCATGG
AGCAGGAGCAGATCGCCTTTGTCTGCAACACCAGCATCGGCGGGGCCGATTACCCGGTCGAGAAGCTCAAGAGCGAGTTT
GACGCCGTGGTCATGGCTACCGGCGCCACCCTCCCCCGCGACCTCAACATCGAGGGGCGCTCGCTTCAGGGTATCCGCTT
CGCCATGGATTTCCTGACCGCCAACACCAAGGCGGTGCTGGAAAAAGGGAGCAAGTTCATCTCGGCGCAAGGGAAAGACG
TGATCATCATCGGCGGGGGCGACACCGGCACCGACTGCGTGGCCACCTCGCTGCGCCATGGCTGCAACTCCGTTACCCAG
CTGGAAATCATGCCCCGTTCCCCCGACACCCGCGCCGATGACAACCGTTGGCCGGAATGGCCCAAGACCCACAAGGTCGA
TTACGGGCAGGAGGAGGCTGCGGCGAAATTCGGCGCCGACCCGCGCGTTTTCCTCACCACCGCGACCAAGTTCGAAGGGG
ATGCGGAGGGCAAGGTGAAGGCGGTGCACACGGTCCAGGTGAAATGGGAGAAGAACGCCCAGGGCCAGTTCGTCCCCACC
CCGGTGCCGGGGACCGAAGAGGTCCGGCCGGCAAGCCTCGTGCTGTTGGCTATGGGCTTTCTGGGCCCCGAGCAGGAACT
GCCGGCGGCACTCGGGCTTGAGCGTGACGGGCGCAGCAACATCAAGGCCGACTACGGCCGCTACGCCACCAACATCCCCG
GCGTGTTCGCAGCCGGCGACTGCCGCCGCGGCCAGAGCCTCGTGGTCTGGGCCTTCAACGAAGGACGCGGGGCAGCGCGC
GAGTGCGACCGTTTCCTGATGGGAGACACCGAGCTTCCCTAA

Upstream 100 bases:

>100_bases
GGATTGAGCGGCGACGAAGCCCTGACAGCGGCATTCGAGGAGAATGCCGGCGCGGCCGCACACTAAACTAACGAGCAAAT
CTTGAGAATAGGATAGAGCT

Downstream 100 bases:

>100_bases
AGCTGACGAGCACCTGTCGGCACGCATGCAAACATGAAGGCACCCGGCCTATTCGGCGGGTGCCTTTTCTTGTGCCGTTT
ACCACTCATCGTTTATCAAA

Product: NAD(P)H-dependent glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGKPTGFMEYLRELPSDREPLERLKDWDEFHLHLPEEKLRTQGARCMDCGIPFCHTGKLVSGMACGCPVNNLIPEFNDLV
YRGLWKQAYDRLSRTNNFPEFTGRVCPAPCEGSCTLGASEPAVTIKNIEVSIIERAWDEGWVTPTLPQVRTGKKVAVVGS
GPAGLSAAEQLNKAGHQVTVFERAPLPGGLLMYGIPNMKLDKQNIVMRRIRLMEQEQIAFVCNTSIGGADYPVEKLKSEF
DAVVMATGATLPRDLNIEGRSLQGIRFAMDFLTANTKAVLEKGSKFISAQGKDVIIIGGGDTGTDCVATSLRHGCNSVTQ
LEIMPRSPDTRADDNRWPEWPKTHKVDYGQEEAAAKFGADPRVFLTTATKFEGDAEGKVKAVHTVQVKWEKNAQGQFVPT
PVPGTEEVRPASLVLLAMGFLGPEQELPAALGLERDGRSNIKADYGRYATNIPGVFAAGDCRRGQSLVVWAFNEGRGAAR
ECDRFLMGDTELP

Sequences:

>Translated_493_residues
MGKPTGFMEYLRELPSDREPLERLKDWDEFHLHLPEEKLRTQGARCMDCGIPFCHTGKLVSGMACGCPVNNLIPEFNDLV
YRGLWKQAYDRLSRTNNFPEFTGRVCPAPCEGSCTLGASEPAVTIKNIEVSIIERAWDEGWVTPTLPQVRTGKKVAVVGS
GPAGLSAAEQLNKAGHQVTVFERAPLPGGLLMYGIPNMKLDKQNIVMRRIRLMEQEQIAFVCNTSIGGADYPVEKLKSEF
DAVVMATGATLPRDLNIEGRSLQGIRFAMDFLTANTKAVLEKGSKFISAQGKDVIIIGGGDTGTDCVATSLRHGCNSVTQ
LEIMPRSPDTRADDNRWPEWPKTHKVDYGQEEAAAKFGADPRVFLTTATKFEGDAEGKVKAVHTVQVKWEKNAQGQFVPT
PVPGTEEVRPASLVLLAMGFLGPEQELPAALGLERDGRSNIKADYGRYATNIPGVFAAGDCRRGQSLVVWAFNEGRGAAR
ECDRFLMGDTELP
>Mature_492_residues
GKPTGFMEYLRELPSDREPLERLKDWDEFHLHLPEEKLRTQGARCMDCGIPFCHTGKLVSGMACGCPVNNLIPEFNDLVY
RGLWKQAYDRLSRTNNFPEFTGRVCPAPCEGSCTLGASEPAVTIKNIEVSIIERAWDEGWVTPTLPQVRTGKKVAVVGSG
PAGLSAAEQLNKAGHQVTVFERAPLPGGLLMYGIPNMKLDKQNIVMRRIRLMEQEQIAFVCNTSIGGADYPVEKLKSEFD
AVVMATGATLPRDLNIEGRSLQGIRFAMDFLTANTKAVLEKGSKFISAQGKDVIIIGGGDTGTDCVATSLRHGCNSVTQL
EIMPRSPDTRADDNRWPEWPKTHKVDYGQEEAAAKFGADPRVFLTTATKFEGDAEGKVKAVHTVQVKWEKNAQGQFVPTP
VPGTEEVRPASLVLLAMGFLGPEQELPAALGLERDGRSNIKADYGRYATNIPGVFAAGDCRRGQSLVVWAFNEGRGAARE
CDRFLMGDTELP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=328, Percent_Identity=27.1341463414634, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=36.0995850622407, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1788811, Length=492, Percent_Identity=35.3658536585366, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI87082180, Length=482, Percent_Identity=32.1576763485477, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=26.9487750556793, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789243, Length=479, Percent_Identity=26.5135699373695, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17570289, Length=502, Percent_Identity=47.808764940239, Blast_Score=466, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI71984108, Length=310, Percent_Identity=25.4838709677419, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543860, Length=156, Percent_Identity=29.4871794871795, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=495, Percent_Identity=49.6969696969697, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=55.9183673469388, Blast_Score=556, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=55.9183673469388, Blast_Score=556, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24665547, Length=489, Percent_Identity=55.8282208588957, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24665543, Length=489, Percent_Identity=55.8282208588957, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24640763, Length=310, Percent_Identity=28.3870967741935, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI18858217, Length=310, Percent_Identity=28.3870967741935, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17137178, Length=157, Percent_Identity=28.6624203821656, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54002; Mature: 53871

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFMEYLRELPSDREPLERLKDWDEFHLHLPEEKLRTQGARCMDCGIPFCHTGKLV
CCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEECCHHHHHHCCCEEEECCCCCEECCCHH
SGMACGCPVNNLIPEFNDLVYRGLWKQAYDRLSRTNNFPEFTGRVCPAPCEGSCTLGASE
CCEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEECCCC
PAVTIKNIEVSIIERAWDEGWVTPTLPQVRTGKKVAVVGSGPAGLSAAEQLNKAGHQVTV
CEEEEEEEEEEEEHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
FERAPLPGGLLMYGIPNMKLDKQNIVMRRIRLMEQEQIAFVCNTSIGGADYPVEKLKSEF
EECCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHH
DAVVMATGATLPRDLNIEGRSLQGIRFAMDFLTANTKAVLEKGSKFISAQGKDVIIIGGG
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCEEECCCCEEEEEECC
DTGTDCVATSLRHGCNSVTQLEIMPRSPDTRADDNRWPEWPKTHKVDYGQEEAAAKFGAD
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
PRVFLTTATKFEGDAEGKVKAVHTVQVKWEKNAQGQFVPTPVPGTEEVRPASLVLLAMGF
CEEEEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
LGPEQELPAALGLERDGRSNIKADYGRYATNIPGVFAAGDCRRGQSLVVWAFNEGRGAAR
CCCHHHCCHHHCCCCCCCCCCCHHHCCHHCCCCCEEECCCCCCCCEEEEEEEECCCCCHH
ECDRFLMGDTELP
HHHHHHCCCCCCC
>Mature Secondary Structure 
GKPTGFMEYLRELPSDREPLERLKDWDEFHLHLPEEKLRTQGARCMDCGIPFCHTGKLV
CCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEECCHHHHHHCCCEEEECCCCCEECCCHH
SGMACGCPVNNLIPEFNDLVYRGLWKQAYDRLSRTNNFPEFTGRVCPAPCEGSCTLGASE
CCEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEECCCC
PAVTIKNIEVSIIERAWDEGWVTPTLPQVRTGKKVAVVGSGPAGLSAAEQLNKAGHQVTV
CEEEEEEEEEEEEHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
FERAPLPGGLLMYGIPNMKLDKQNIVMRRIRLMEQEQIAFVCNTSIGGADYPVEKLKSEF
EECCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHH
DAVVMATGATLPRDLNIEGRSLQGIRFAMDFLTANTKAVLEKGSKFISAQGKDVIIIGGG
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCEEECCCCEEEEEECC
DTGTDCVATSLRHGCNSVTQLEIMPRSPDTRADDNRWPEWPKTHKVDYGQEEAAAKFGAD
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
PRVFLTTATKFEGDAEGKVKAVHTVQVKWEKNAQGQFVPTPVPGTEEVRPASLVLLAMGF
CEEEEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
LGPEQELPAALGLERDGRSNIKADYGRYATNIPGVFAAGDCRRGQSLVVWAFNEGRGAAR
CCCHHHCCHHHCCCCCCCCCCCHHHCCHHCCCCCEEECCCCCCCCEEEEEEEECCCCCHH
ECDRFLMGDTELP
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]