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The map label for this gene is nfsA [C]

Identifier: 197119210

GI number: 197119210

Start: 3264146

End: 3264730

Strand: Reverse

Name: nfsA [C]

Synonym: Gbem_2837

Alternate gene names: 197119210

Gene position: 3264730-3264146 (Counterclockwise)

Preceding gene: 197119211

Following gene: 197119209

Centisome position: 70.74

GC content: 58.29

Gene sequence:

>585_bases
ATGGACGTATTCGAGGCAATCAACGGCAGAAGGAGCATCCGGAAGTACAAGGACACTCCGGTCGAATGGGAGAAGGTGGG
GCAGGTGCTGGATGCAGCCAGGCTTGCCCCTTCCTGGAAGAACATGCAGTGCTGGCGCTTTCTGGTTCTGAACTCTACAG
AGAGGAAAAAGGCGCTCCTCGACGGCTTTTCGGACGATAATCCTGGGAAGAAGGCGCTGGCCCAGGCGCCGGTGGTCATT
GTCGTCTGCGCCGACCCGACTCAATCGGGCTTAGAGAACGGCATCGAGTACTACGTAGCCGACGCAGCCATCGCCTTCGA
ACATATCTGTCTCGCGGCGCACGGCCTAGGTCTTGGCACCTGCTGGATGGGATGGTATGATGAGCCCGCCATCAAGGCGG
GGCTGGCCATTCCGGCCGGTATCAGGGTCATCGGCGTGACCCCGCTCGGGTATCCCGACCAGGATCCCAAACAGCGCCCG
CGCAAGGATTTGAATGAAATTGCCTACTACAACGATTGGGGGGTGACGGATGCTGAACAAGCAGGAATCGCAACAGAGAA
TCACCAGGCTGCAACAGGAACTTAA

Upstream 100 bases:

>100_bases
CTCCCCCTCCCTTTACGGGAGGGGGGAGTTTGTGCTGGGCGGATCAACCATGGAGAAGCCGACGCCAAAGCGATGGACAG
GAACCGGATAAGGAGAACGC

Downstream 100 bases:

>100_bases
GGCAAAGGGTATCGACGGAGCGCTCTTCATCTACCCCATCGACGTCTACTATTTCACCGGCACCCGCCAGAATTCGACGC
TCTGGGTCCCGGCCGAGGGG

Product: nitroreductase-like family 3 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MDVFEAINGRRSIRKYKDTPVEWEKVGQVLDAARLAPSWKNMQCWRFLVLNSTERKKALLDGFSDDNPGKKALAQAPVVI
VVCADPTQSGLENGIEYYVADAAIAFEHICLAAHGLGLGTCWMGWYDEPAIKAGLAIPAGIRVIGVTPLGYPDQDPKQRP
RKDLNEIAYYNDWGVTDAEQAGIATENHQAATGT

Sequences:

>Translated_194_residues
MDVFEAINGRRSIRKYKDTPVEWEKVGQVLDAARLAPSWKNMQCWRFLVLNSTERKKALLDGFSDDNPGKKALAQAPVVI
VVCADPTQSGLENGIEYYVADAAIAFEHICLAAHGLGLGTCWMGWYDEPAIKAGLAIPAGIRVIGVTPLGYPDQDPKQRP
RKDLNEIAYYNDWGVTDAEQAGIATENHQAATGT
>Mature_194_residues
MDVFEAINGRRSIRKYKDTPVEWEKVGQVLDAARLAPSWKNMQCWRFLVLNSTERKKALLDGFSDDNPGKKALAQAPVVI
VVCADPTQSGLENGIEYYVADAAIAFEHICLAAHGLGLGTCWMGWYDEPAIKAGLAIPAGIRVIGVTPLGYPDQDPKQRP
RKDLNEIAYYNDWGVTDAEQAGIATENHQAATGT

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 21260; Mature: 21260

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVFEAINGRRSIRKYKDTPVEWEKVGQVLDAARLAPSWKNMQCWRFLVLNSTERKKALL
CCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHH
DGFSDDNPGKKALAQAPVVIVVCADPTQSGLENGIEYYVADAAIAFEHICLAAHGLGLGT
CCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHCHHEEHHHHHHHHHHHHHHHCCCCCCC
CWMGWYDEPAIKAGLAIPAGIRVIGVTPLGYPDQDPKQRPRKDLNEIAYYNDWGVTDAEQ
EECCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCEECCCCCCCHHH
AGIATENHQAATGT
CCCCCCCCCCCCCC
>Mature Secondary Structure
MDVFEAINGRRSIRKYKDTPVEWEKVGQVLDAARLAPSWKNMQCWRFLVLNSTERKKALL
CCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHH
DGFSDDNPGKKALAQAPVVIVVCADPTQSGLENGIEYYVADAAIAFEHICLAAHGLGLGT
CCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHCHHEEHHHHHHHHHHHHHHHCCCCCCC
CWMGWYDEPAIKAGLAIPAGIRVIGVTPLGYPDQDPKQRPRKDLNEIAYYNDWGVTDAEQ
EECCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCEECCCCCCCHHH
AGIATENHQAATGT
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]