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The map label for this gene is thlA [H]

Identifier: 197119208

GI number: 197119208

Start: 3261476

End: 3262651

Strand: Reverse

Name: thlA [H]

Synonym: Gbem_2835

Alternate gene names: 197119208

Gene position: 3262651-3261476 (Counterclockwise)

Preceding gene: 197119209

Following gene: 197119207

Centisome position: 70.69

GC content: 65.05

Gene sequence:

>1176_bases
ATGAAAGACATCTTTGTCGTTGAGTCGCAGAGAACCCCGTTCGGATCTTTCGGCGGCGTTCTCTCGGATGTTGAGGCTAC
GGTCCTTGGGGGGGGCGCCATCAAGGCTCTCCTGGAGCGGACCGGCCTGGACCCGGCCGAGGTCGACGAGGTCATCGCCG
GGCAGGTTCTTTCCGGCGGCTGCGGGCAGGCTCCTGCGCGCCAGGCGATGCGCGGCGCCGGGATCCCGGACAGCGCCCAT
GCCATGACCATCAACAAGGTCTGCGGCAGCGGCTTAAAAGCGATCATGCTGGGGGCAGGCTCCATCCAGCTGGAAGATTC
CGAAATCGTTGTGGCCGGCGGCATGGAAAACATGTCCCTCGCGCCCTTTCTGCTCAAGAAGGCGCGCTACGGCTACCGCA
TGGGGCACGGCTCGCTCATCGACCTGATGATTTACGACGGCCTGCAGGACCCATACTCCGGGCGGCACATGGGAGAGATC
GCCGAGGCTTCGGTGAAAAAGCATGGGCTCACCCGCGAGGCGCAGGACGAATTCACGCTGCGCTCCTACAAGCTGGCCCA
GGACGCGGTGCGCGGCGGGGTCTTCAAGGACGAGATCGCGCCGGTGGTGAAAAAAGGGCGCAAGGGGGACGAGGTGATCT
CGGACGACGAGGAGCCGTTCAAGGTGGACTTCGACAAGCTGACGCAGTTGAGGCCGGTGTTCAGCAAGGACGGGACCATC
ACCGCCGCCAACGCCTCCAGCATCAACGACGGCGCAGCCTTCACGCTCCTGGCTGGGGAGTCGGCGCTGAAGCGCTGCAA
CCTTACCCCGAAGGCGCGCGTGGTTGCCTACGCCACCGGTAGCCTGCACCCGGAGCTCTACACCGAAGCCCCCGTGACGG
CGATCCAGGCGGCCTGCGCCAGGGCCGGACTGAAAGTGGGGGACATCGACCTCTTCGAGATCAACGAAGCTTTCGCCGCG
GTGACCATGATTGCCATGAAAGAACTTGATTTGAACCCCGCCAAAGTCAACGTCAACGGCGGAGCCTGCGCCCTGGGGCA
CCCCATCGGCGCGAGCGGCGCACGGCTTGCCGGGACGCTGATCCGGGAGCTGCACAAGCGGCAGGCGCGCTATGGGCTCG
CCACGCTCTGCATCGGGGGCGGCGAGGCGGTCGCGGTCATTTTCGAAAGGGTCTAG

Upstream 100 bases:

>100_bases
CAACTCGTGCTAAATTCCTAAAAACCTGTTCTAGAAATGCTTGACATGCACGTAAACGACATATATATTCGCATCACAAA
TAACTGCTGTTGGAGGTGAC

Downstream 100 bases:

>100_bases
ACACTTTTCTCGCAGGGGGGAGGCTTACATGTTCAAGACAGTCGGTGTTTTAGGGGCGGGGCAGATGGGAAGCGGGATCG
CCCACGTTTTCGCGCAGCAC

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKDIFVVESQRTPFGSFGGVLSDVEATVLGGGAIKALLERTGLDPAEVDEVIAGQVLSGGCGQAPARQAMRGAGIPDSAH
AMTINKVCGSGLKAIMLGAGSIQLEDSEIVVAGGMENMSLAPFLLKKARYGYRMGHGSLIDLMIYDGLQDPYSGRHMGEI
AEASVKKHGLTREAQDEFTLRSYKLAQDAVRGGVFKDEIAPVVKKGRKGDEVISDDEEPFKVDFDKLTQLRPVFSKDGTI
TAANASSINDGAAFTLLAGESALKRCNLTPKARVVAYATGSLHPELYTEAPVTAIQAACARAGLKVGDIDLFEINEAFAA
VTMIAMKELDLNPAKVNVNGGACALGHPIGASGARLAGTLIRELHKRQARYGLATLCIGGGEAVAVIFERV

Sequences:

>Translated_391_residues
MKDIFVVESQRTPFGSFGGVLSDVEATVLGGGAIKALLERTGLDPAEVDEVIAGQVLSGGCGQAPARQAMRGAGIPDSAH
AMTINKVCGSGLKAIMLGAGSIQLEDSEIVVAGGMENMSLAPFLLKKARYGYRMGHGSLIDLMIYDGLQDPYSGRHMGEI
AEASVKKHGLTREAQDEFTLRSYKLAQDAVRGGVFKDEIAPVVKKGRKGDEVISDDEEPFKVDFDKLTQLRPVFSKDGTI
TAANASSINDGAAFTLLAGESALKRCNLTPKARVVAYATGSLHPELYTEAPVTAIQAACARAGLKVGDIDLFEINEAFAA
VTMIAMKELDLNPAKVNVNGGACALGHPIGASGARLAGTLIRELHKRQARYGLATLCIGGGEAVAVIFERV
>Mature_391_residues
MKDIFVVESQRTPFGSFGGVLSDVEATVLGGGAIKALLERTGLDPAEVDEVIAGQVLSGGCGQAPARQAMRGAGIPDSAH
AMTINKVCGSGLKAIMLGAGSIQLEDSEIVVAGGMENMSLAPFLLKKARYGYRMGHGSLIDLMIYDGLQDPYSGRHMGEI
AEASVKKHGLTREAQDEFTLRSYKLAQDAVRGGVFKDEIAPVVKKGRKGDEVISDDEEPFKVDFDKLTQLRPVFSKDGTI
TAANASSINDGAAFTLLAGESALKRCNLTPKARVVAYATGSLHPELYTEAPVTAIQAACARAGLKVGDIDLFEINEAFAA
VTMIAMKELDLNPAKVNVNGGACALGHPIGASGARLAGTLIRELHKRQARYGLATLCIGGGEAVAVIFERV

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=45.29262086514, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=45.3846153846154, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI167614485, Length=391, Percent_Identity=40.4092071611253, Blast_Score=295, Evalue=3e-80,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=36.5239294710327, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=30.6791569086651, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=28.9340101522843, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=46.2915601023018, Blast_Score=344, Evalue=7e-96,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=49.8727735368957, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=40.2985074626866, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI48994986, Length=409, Percent_Identity=36.4303178484108, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=29.2682926829268, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=43.7340153452685, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=43.8461538461538, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17535921, Length=392, Percent_Identity=41.0714285714286, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17535917, Length=400, Percent_Identity=32.75, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=30.8962264150943, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325229, Length=396, Percent_Identity=43.9393939393939, Blast_Score=313, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=34.5088161209068, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24640423, Length=393, Percent_Identity=45.5470737913486, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=45.4081632653061, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=40.1015228426396, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=31.1320754716981, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41091; Mature: 41091

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIFVVESQRTPFGSFGGVLSDVEATVLGGGAIKALLERTGLDPAEVDEVIAGQVLSGG
CCCEEEEECCCCCCHHHCCHHHHCHHEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
CGQAPARQAMRGAGIPDSAHAMTINKVCGSGLKAIMLGAGSIQLEDSEIVVAGGMENMSL
CCCCHHHHHHHCCCCCCCCCEEEHHHHHCCCCEEEEEECCCEEEECCCEEEECCCCCCCH
APFLLKKARYGYRMGHGSLIDLMIYDGLQDPYSGRHMGEIAEASVKKHGLTREAQDEFTL
HHHHHHHHHCCEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
RSYKLAQDAVRGGVFKDEIAPVVKKGRKGDEVISDDEEPFKVDFDKLTQLRPVFSKDGTI
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCE
TAANASSINDGAAFTLLAGESALKRCNLTPKARVVAYATGSLHPELYTEAPVTAIQAACA
EECCCCCCCCCCEEEEEECHHHHHHCCCCCCCEEEEEECCCCCCCHHCCCCHHHHHHHHH
RAGLKVGDIDLFEINEAFAAVTMIAMKELDLNPAKVNVNGGACALGHPIGASGARLAGTL
HCCCEECCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCHHHHHHH
IRELHKRQARYGLATLCIGGGEAVAVIFERV
HHHHHHHHHHCCEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MKDIFVVESQRTPFGSFGGVLSDVEATVLGGGAIKALLERTGLDPAEVDEVIAGQVLSGG
CCCEEEEECCCCCCHHHCCHHHHCHHEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
CGQAPARQAMRGAGIPDSAHAMTINKVCGSGLKAIMLGAGSIQLEDSEIVVAGGMENMSL
CCCCHHHHHHHCCCCCCCCCEEEHHHHHCCCCEEEEEECCCEEEECCCEEEECCCCCCCH
APFLLKKARYGYRMGHGSLIDLMIYDGLQDPYSGRHMGEIAEASVKKHGLTREAQDEFTL
HHHHHHHHHCCEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
RSYKLAQDAVRGGVFKDEIAPVVKKGRKGDEVISDDEEPFKVDFDKLTQLRPVFSKDGTI
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCE
TAANASSINDGAAFTLLAGESALKRCNLTPKARVVAYATGSLHPELYTEAPVTAIQAACA
EECCCCCCCCCCEEEEEECHHHHHHCCCCCCCEEEEEECCCCCCCHHCCCCHHHHHHHHH
RAGLKVGDIDLFEINEAFAAVTMIAMKELDLNPAKVNVNGGACALGHPIGASGARLAGTL
HCCCEECCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCHHHHHHH
IRELHKRQARYGLATLCIGGGEAVAVIFERV
HHHHHHHHHHCCEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]