Click here to switch to the map view.

The map label for this gene is fadF [H]

Identifier: 197119204

GI number: 197119204

Start: 3256316

End: 3258460

Strand: Reverse

Name: fadF [H]

Synonym: Gbem_2831

Alternate gene names: 197119204

Gene position: 3258460-3256316 (Counterclockwise)

Preceding gene: 197119205

Following gene: 197119203

Centisome position: 70.6

GC content: 62.61

Gene sequence:

>2145_bases
ATGGAAGCTACCAGGGAAATCTATTGGAACGTCGGACACGGGGTCGTGCTCCCCATGTACCTGCTGACGCTGGTCGCCGT
CGCCGCCTGCTGTTACGGCATCTACCAGCGGGTCGAGGTGTACCGCCAGGGGAAGGAACTGAACCGGCTCGACGCCCTGG
GGGAACGTATCCCCTTCCTGCTCAAGAACCTGTTCGGCCAGGCGAAGGTGCTGCGCGTCAGCGGTCCCGGCTTTCCCCAC
GGCCTCTTCTTCTGGGGCTTTGTCACCCTCTTCATCGGGACGCTGCTGGTGATGCTCCAGGCCGACCTGACCCAGCCGCT
CTTCAATGTGGTGATCCTCAAGGGCTCCTTCTATAAGTTCTATTCCCTGGCGCTCGACCTGGCAGGCGCTGCCGCCTTCC
TCATGCTTTTGGGCCTGATGCTGCGCCGCTTTGTCTACCGGCCGGAGGGGCTCAAGATCACCCGCGACGATTACCTGATG
CATGGCCTCCTGACCGCGATCATCTTCACCGGATTCCTGGCGGAAGGTGTGCGCATCGCCGCGACCGAACTGCGGATGAA
TCCTGAGCTGGCGCGCTGGTCCCCCATCGGGATGTTCGTGGCCAACCAGTTCTCCGCCCAGGACGAGGTTTCCCTGCGGC
AGCTGCACACGCTCTGGTGGTGGGGTCATTTCCTGCTGGTCCTCGGGTTCATCGTCTCTATTCCCTGGAGCAAATTCCGT
CACTTGATCACCACGCCGGCCAACTACCTCTTCACCGACCTCCGCCCCAAGGGGTCCATCGCCACCATAAACCTGGAAGA
GGAGGGGGTGGAGCAGTTCGGCGTGGAGAAGGTTTCGGAATTCTCCTGGAAGGACATCTTCGACGCCGACGCCTGCACCT
CCTGCAAGAGATGCCAGGACCGCTGCCCGGCCCACAACACCGAGAAGCCGCTCTCCCCGATGAAGGTGGTGCAGCAGGTA
GGCGAGTTGGCCTTCGGCGACCCCGACAAGAGCCTGATCGAGACGGTCGGCAACGACGCGCTCTGGTCCTGCACCACCTG
CCGCGCCTGCCAGGAGATCTGCCCGGCGGATGTGGAGCATGTGAACAAGATCATCGAGATGCGCCGCAACCTGGTCCTGA
TGCAGGGGGAGTTCCCCGGCGAGGAGGTCATGGTCGCCGTCAACAACGTCGAGGTGAACGGCAACCCCTTCGGCCTCGCC
TACGCCGGCCGCGGGGATTGGACCGAGGGGCTGGATGTGCAGATCCTGTCGGAGGGTGGGGCTGACGTGGACGTGCTGTA
CTTCGTCGGCTGCTACGCCTCCTTCGACAAGCGAAACCAGGAGGTCGCCAAAAGCTTCGTTAAGATCTGCAACGCTGCGG
GGATCAAGGTTGGCATCCTCGGCAAGGAGGAGAAGTGCTGCGGGGAGCCGGTCAGGAAGCTCGGCAACGAGTACCTGTAC
CAGATGGCGGCCCAGGAGAACATCGAACTGATCAACGGGTACGGCATCAAGCGGATCGTCACCACCTGCCCGCACTGCAT
GAACACGCTCGGGCGCGACTACCGGGACCTGGGGCTGGATGCGCAGGTGGAGCACTACACGGTCTTCATCGACCGCCTGC
TCAAGGAGGGGACCCTTGTCCTCGACGAGCGGCAGTTCGACTACACCTACCACGATTCCTGCTATCTCGGGCGCTACCAG
GACATCATCCAGCAGCCGCGCGACATACTGCACGCCGCGGGCGGGCGCATCAACGAGATGGAGAAATCGGAAAAGGAAAG
CTTTTGCTGCGGCGCAGGGGGGGGCAGGATCCTCGCCGAGGAGAAACTGGGGAACAGGATCAACGTGAAGAGGGTCCACA
TGGCGGAAGCGACCGGGGCACCCGTCCTGGTATCCAACTGCCCCTTCTGCCTCACCATGTTCGAAGACGGCATCAAGACC
GGCGAGTGCGAAGGGAAGCTGAAGGCTCGGGACCTGGCCGAGATCGTCGTGGAGAGGCTGCAGCCGGCAGCCGACCTCTC
CGCGGTGCTCGACACCTTCTTGCCCACAGGGATTCCGCAGGTTACGGAAGCTTCGGCGGCAGGGGCGGAACCGGTTTCGG
CAGAGACAGAAAAAGCGGGAGAAACCACAGAGATTTCGGGTGTAAACGGCAAGGACGCTGCCTGA

Upstream 100 bases:

>100_bases
TAGGGGAGGGGGGACTTTGGGACAACGCTGGGCGTCGTCGGGTTGCTGCCCGGGAAGGCGGCTCCCTCAGGAGAGATCAA
TAAACGCGCAGGAGTCGACC

Downstream 100 bases:

>100_bases
ACCGCATCGACAAGCGGCGGCAAAGCCGCAGCTACAAAAGAGTCAACAAGGAGGAGGCCATGAAGATACTGGTATGCATC
AAGCAGGTTCCTGACATGGA

Product: iron-sulfur cluster-binding oxidoreductase lipoprotein

Products: FADH2; dihydroxy-acetone-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MEATREIYWNVGHGVVLPMYLLTLVAVAACCYGIYQRVEVYRQGKELNRLDALGERIPFLLKNLFGQAKVLRVSGPGFPH
GLFFWGFVTLFIGTLLVMLQADLTQPLFNVVILKGSFYKFYSLALDLAGAAAFLMLLGLMLRRFVYRPEGLKITRDDYLM
HGLLTAIIFTGFLAEGVRIAATELRMNPELARWSPIGMFVANQFSAQDEVSLRQLHTLWWWGHFLLVLGFIVSIPWSKFR
HLITTPANYLFTDLRPKGSIATINLEEEGVEQFGVEKVSEFSWKDIFDADACTSCKRCQDRCPAHNTEKPLSPMKVVQQV
GELAFGDPDKSLIETVGNDALWSCTTCRACQEICPADVEHVNKIIEMRRNLVLMQGEFPGEEVMVAVNNVEVNGNPFGLA
YAGRGDWTEGLDVQILSEGGADVDVLYFVGCYASFDKRNQEVAKSFVKICNAAGIKVGILGKEEKCCGEPVRKLGNEYLY
QMAAQENIELINGYGIKRIVTTCPHCMNTLGRDYRDLGLDAQVEHYTVFIDRLLKEGTLVLDERQFDYTYHDSCYLGRYQ
DIIQQPRDILHAAGGRINEMEKSEKESFCCGAGGGRILAEEKLGNRINVKRVHMAEATGAPVLVSNCPFCLTMFEDGIKT
GECEGKLKARDLAEIVVERLQPAADLSAVLDTFLPTGIPQVTEASAAGAEPVSAETEKAGETTEISGVNGKDAA

Sequences:

>Translated_714_residues
MEATREIYWNVGHGVVLPMYLLTLVAVAACCYGIYQRVEVYRQGKELNRLDALGERIPFLLKNLFGQAKVLRVSGPGFPH
GLFFWGFVTLFIGTLLVMLQADLTQPLFNVVILKGSFYKFYSLALDLAGAAAFLMLLGLMLRRFVYRPEGLKITRDDYLM
HGLLTAIIFTGFLAEGVRIAATELRMNPELARWSPIGMFVANQFSAQDEVSLRQLHTLWWWGHFLLVLGFIVSIPWSKFR
HLITTPANYLFTDLRPKGSIATINLEEEGVEQFGVEKVSEFSWKDIFDADACTSCKRCQDRCPAHNTEKPLSPMKVVQQV
GELAFGDPDKSLIETVGNDALWSCTTCRACQEICPADVEHVNKIIEMRRNLVLMQGEFPGEEVMVAVNNVEVNGNPFGLA
YAGRGDWTEGLDVQILSEGGADVDVLYFVGCYASFDKRNQEVAKSFVKICNAAGIKVGILGKEEKCCGEPVRKLGNEYLY
QMAAQENIELINGYGIKRIVTTCPHCMNTLGRDYRDLGLDAQVEHYTVFIDRLLKEGTLVLDERQFDYTYHDSCYLGRYQ
DIIQQPRDILHAAGGRINEMEKSEKESFCCGAGGGRILAEEKLGNRINVKRVHMAEATGAPVLVSNCPFCLTMFEDGIKT
GECEGKLKARDLAEIVVERLQPAADLSAVLDTFLPTGIPQVTEASAAGAEPVSAETEKAGETTEISGVNGKDAA
>Mature_714_residues
MEATREIYWNVGHGVVLPMYLLTLVAVAACCYGIYQRVEVYRQGKELNRLDALGERIPFLLKNLFGQAKVLRVSGPGFPH
GLFFWGFVTLFIGTLLVMLQADLTQPLFNVVILKGSFYKFYSLALDLAGAAAFLMLLGLMLRRFVYRPEGLKITRDDYLM
HGLLTAIIFTGFLAEGVRIAATELRMNPELARWSPIGMFVANQFSAQDEVSLRQLHTLWWWGHFLLVLGFIVSIPWSKFR
HLITTPANYLFTDLRPKGSIATINLEEEGVEQFGVEKVSEFSWKDIFDADACTSCKRCQDRCPAHNTEKPLSPMKVVQQV
GELAFGDPDKSLIETVGNDALWSCTTCRACQEICPADVEHVNKIIEMRRNLVLMQGEFPGEEVMVAVNNVEVNGNPFGLA
YAGRGDWTEGLDVQILSEGGADVDVLYFVGCYASFDKRNQEVAKSFVKICNAAGIKVGILGKEEKCCGEPVRKLGNEYLY
QMAAQENIELINGYGIKRIVTTCPHCMNTLGRDYRDLGLDAQVEHYTVFIDRLLKEGTLVLDERQFDYTYHDSCYLGRYQ
DIIQQPRDILHAAGGRINEMEKSEKESFCCGAGGGRILAEEKLGNRINVKRVHMAEATGAPVLVSNCPFCLTMFEDGIKT
GECEGKLKARDLAEIVVERLQPAADLSAVLDTFLPTGIPQVTEASAAGAEPVSAETEKAGETTEISGVNGKDAA

Specific function: Electron Transfer Protein; May Also Function As The Membrane Anchor For The Glpab Dimer. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=388, Percent_Identity=22.9381443298969, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR009051 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 79288; Mature: 79288

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATREIYWNVGHGVVLPMYLLTLVAVAACCYGIYQRVEVYRQGKELNRLDALGERIPFL
CCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LKNLFGQAKVLRVSGPGFPHGLFFWGFVTLFIGTLLVMLQADLTQPLFNVVILKGSFYKF
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHH
YSLALDLAGAAAFLMLLGLMLRRFVYRPEGLKITRDDYLMHGLLTAIIFTGFLAEGVRIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCEEE
ATELRMNPELARWSPIGMFVANQFSAQDEVSLRQLHTLWWWGHFLLVLGFIVSIPWSKFR
HHHHHCCCCHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HLITTPANYLFTDLRPKGSIATINLEEEGVEQFGVEKVSEFSWKDIFDADACTSCKRCQD
HHHCCCHHHHEECCCCCCCEEEEECCHHHHHHHCHHHHHHCCHHHCCCCHHHHHHHHHHH
RCPAHNTEKPLSPMKVVQQVGELAFGDPDKSLIETVGNDALWSCTTCRACQEICPADVEH
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHH
VNKIIEMRRNLVLMQGEFPGEEVMVAVNNVEVNGNPFGLAYAGRGDWTEGLDVQILSEGG
HHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCCEEEECCCC
ADVDVLYFVGCYASFDKRNQEVAKSFVKICNAAGIKVGILGKEEKCCGEPVRKLGNEYLY
CCEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
QMAAQENIELINGYGIKRIVTTCPHCMNTLGRDYRDLGLDAQVEHYTVFIDRLLKEGTLV
HHHHHCCCEEECCCCHHHHHHHCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCEE
LDERQFDYTYHDSCYLGRYQDIIQQPRDILHAAGGRINEMEKSEKESFCCGAGGGRILAE
EECCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEECCCCCCEEEH
EKLGNRINVKRVHMAEATGAPVLVSNCPFCLTMFEDGIKTGECEGKLKARDLAEIVVERL
HHCCCCCCEEEEEEECCCCCCEEECCCCEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
QPAADLSAVLDTFLPTGIPQVTEASAAGAEPVSAETEKAGETTEISGVNGKDAA
CCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHCCCCCEECCCCCCCCC
>Mature Secondary Structure
MEATREIYWNVGHGVVLPMYLLTLVAVAACCYGIYQRVEVYRQGKELNRLDALGERIPFL
CCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LKNLFGQAKVLRVSGPGFPHGLFFWGFVTLFIGTLLVMLQADLTQPLFNVVILKGSFYKF
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHH
YSLALDLAGAAAFLMLLGLMLRRFVYRPEGLKITRDDYLMHGLLTAIIFTGFLAEGVRIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCEEE
ATELRMNPELARWSPIGMFVANQFSAQDEVSLRQLHTLWWWGHFLLVLGFIVSIPWSKFR
HHHHHCCCCHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HLITTPANYLFTDLRPKGSIATINLEEEGVEQFGVEKVSEFSWKDIFDADACTSCKRCQD
HHHCCCHHHHEECCCCCCCEEEEECCHHHHHHHCHHHHHHCCHHHCCCCHHHHHHHHHHH
RCPAHNTEKPLSPMKVVQQVGELAFGDPDKSLIETVGNDALWSCTTCRACQEICPADVEH
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHH
VNKIIEMRRNLVLMQGEFPGEEVMVAVNNVEVNGNPFGLAYAGRGDWTEGLDVQILSEGG
HHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCCEEEECCCC
ADVDVLYFVGCYASFDKRNQEVAKSFVKICNAAGIKVGILGKEEKCCGEPVRKLGNEYLY
CCEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
QMAAQENIELINGYGIKRIVTTCPHCMNTLGRDYRDLGLDAQVEHYTVFIDRLLKEGTLV
HHHHHCCCEEECCCCHHHHHHHCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCEE
LDERQFDYTYHDSCYLGRYQDIIQQPRDILHAAGGRINEMEKSEKESFCCGAGGGRILAE
EECCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEECCCCCCEEEH
EKLGNRINVKRVHMAEATGAPVLVSNCPFCLTMFEDGIKTGECEGKLKARDLAEIVVERL
HHCCCCCCEEEEEEECCCCCCEEECCCCEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
QPAADLSAVLDTFLPTGIPQVTEASAAGAEPVSAETEKAGETTEISGVNGKDAA
CCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: FAD; glycerol-3-phosphate [C]

Specific reaction: FAD + glycerol-3-phosphate = FADH2 + dihydroxy-acetone-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]