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The map label for this gene is gctB [H]

Identifier: 197119197

GI number: 197119197

Start: 3250077

End: 3250901

Strand: Reverse

Name: gctB [H]

Synonym: Gbem_2824

Alternate gene names: 197119197

Gene position: 3250901-3250077 (Counterclockwise)

Preceding gene: 197119198

Following gene: 197119196

Centisome position: 70.44

GC content: 61.82

Gene sequence:

>825_bases
ATGGAAAGCAATTACGCAACTCCCGAAGAATACGGCCTCCCCGATCTTCTCTGCTGCGCCGCCTCGCGCGAGGTAAAGGA
CAACGAAATCGTCTTCGCCGGCACCGGCCTTCCCATGGTGGCGATCATGCTGGCCCAGAAAACCCACGCCCCCAACCTGA
AGTTGATCTTCGAGGCCGGGACGCTGGACGGGCGCCCACCGCAGATTCCGACCTCGGTAGGCGACGCGCGCTGCGAGATG
GGAGCCTCCCGCTCCTCTGGGCTTTACGACGCCTTCAGCGTGGCCCAAAGAGGGTACGTCGACTTAGGTTTCCTCGGCGG
TGCGGAGGTCGATGAGTACGGCAACGTGAACACCACCTGCATCGGGAACTACCTCGACCCCGAACTCCGCCTCACCGGTT
CCGGCGGCAACCCCGACATCAACTCCTTCGCCCGACGCACCGTCTTTATCATGGTGCACGAGAAGCGCCGCTTCACCGAA
CAGGTGAGCTACATCACCAGCCCTGGATGGCGGGTGAAGAAATGGCCTGAAGGGAGCTTCGTCCACCGCAAGGAACTCTA
CGGCTCCGCCTACCGCGGAGGCCCCTCCGCCGTCATCAGCACGGCCGGCGTCTTCCGCTTCGACGACGAGACCGGGCGCA
TCTACCTAGACACCTGCCACCGGGGTAACTCCCCCGAGCAAATCCGCGAGATGTGTCAATTTGATCTTGACATCTCGCGG
GTTAGCGGCGAAACTCTGCCCCCGACCAGGGAGGAGCTGCACTTCATCCACAGTGTGCTCGACCCTGAGCAGATTTTCAT
TCCCAAGGTGAAGCACAAAGGGTAG

Upstream 100 bases:

>100_bases
CACCATAGCTACCTGGAGAAACTGGGCTGGCCGCGCATGCTGAACCTCAAGGCCAATACCGCGCTCAACTACAGCACCGG
CGTGAAAAGGGGGCAATGAC

Downstream 100 bases:

>100_bases
CAATGGCTGAATTGAACCCGATGGAGGATTTCCAGAGGGACATCTCCTATCGTCTGTTGAAGCAGGATGACGGCACCGCG
AAGCTGACGGCGCTTTTGAA

Product: coenzyme A transferase subunit beta

Products: NA

Alternate protein names: GCT small subunit [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MESNYATPEEYGLPDLLCCAASREVKDNEIVFAGTGLPMVAIMLAQKTHAPNLKLIFEAGTLDGRPPQIPTSVGDARCEM
GASRSSGLYDAFSVAQRGYVDLGFLGGAEVDEYGNVNTTCIGNYLDPELRLTGSGGNPDINSFARRTVFIMVHEKRRFTE
QVSYITSPGWRVKKWPEGSFVHRKELYGSAYRGGPSAVISTAGVFRFDDETGRIYLDTCHRGNSPEQIREMCQFDLDISR
VSGETLPPTREELHFIHSVLDPEQIFIPKVKHKG

Sequences:

>Translated_274_residues
MESNYATPEEYGLPDLLCCAASREVKDNEIVFAGTGLPMVAIMLAQKTHAPNLKLIFEAGTLDGRPPQIPTSVGDARCEM
GASRSSGLYDAFSVAQRGYVDLGFLGGAEVDEYGNVNTTCIGNYLDPELRLTGSGGNPDINSFARRTVFIMVHEKRRFTE
QVSYITSPGWRVKKWPEGSFVHRKELYGSAYRGGPSAVISTAGVFRFDDETGRIYLDTCHRGNSPEQIREMCQFDLDISR
VSGETLPPTREELHFIHSVLDPEQIFIPKVKHKG
>Mature_274_residues
MESNYATPEEYGLPDLLCCAASREVKDNEIVFAGTGLPMVAIMLAQKTHAPNLKLIFEAGTLDGRPPQIPTSVGDARCEM
GASRSSGLYDAFSVAQRGYVDLGFLGGAEVDEYGNVNTTCIGNYLDPELRLTGSGGNPDINSFARRTVFIMVHEKRRFTE
QVSYITSPGWRVKKWPEGSFVHRKELYGSAYRGGPSAVISTAGVFRFDDETGRIYLDTCHRGNSPEQIREMCQFDLDISR
VSGETLPPTREELHFIHSVLDPEQIFIPKVKHKG

Specific function: Catalyzes the transfer of the CoA moiety from acetyl-CoA to (R)-2-hydroxyglutarate and related compounds like glutaconate [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

None

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004165 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.12 [H]

Molecular weight: Translated: 30330; Mature: 30330

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESNYATPEEYGLPDLLCCAASREVKDNEIVFAGTGLPMVAIMLAQKTHAPNLKLIFEAG
CCCCCCCCHHCCCCHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEEC
TLDGRPPQIPTSVGDARCEMGASRSSGLYDAFSVAQRGYVDLGFLGGAEVDEYGNVNTTC
CCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHCCCCCEEE
IGNYLDPELRLTGSGGNPDINSFARRTVFIMVHEKRRFTEQVSYITSPGWRVKKWPEGSF
ECCCCCCCEEEECCCCCCCHHHHHCEEEEEEEECHHHHHHHHHHHCCCCCEEEECCCCCC
VHRKELYGSAYRGGPSAVISTAGVFRFDDETGRIYLDTCHRGNSPEQIREMCQFDLDISR
EEHHHHHCCCCCCCCHHHEECCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCHHH
VSGETLPPTREELHFIHSVLDPEQIFIPKVKHKG
CCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MESNYATPEEYGLPDLLCCAASREVKDNEIVFAGTGLPMVAIMLAQKTHAPNLKLIFEAG
CCCCCCCCHHCCCCHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEEC
TLDGRPPQIPTSVGDARCEMGASRSSGLYDAFSVAQRGYVDLGFLGGAEVDEYGNVNTTC
CCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHCCCCCEEE
IGNYLDPELRLTGSGGNPDINSFARRTVFIMVHEKRRFTEQVSYITSPGWRVKKWPEGSF
ECCCCCCCEEEECCCCCCCHHHHHCEEEEEEEECHHHHHHHHHHHCCCCCEEEECCCCCC
VHRKELYGSAYRGGPSAVISTAGVFRFDDETGRIYLDTCHRGNSPEQIREMCQFDLDISR
EEHHHHHCCCCCCCCHHHEECCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCHHH
VSGETLPPTREELHFIHSVLDPEQIFIPKVKHKG
CCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7957258; 9083111 [H]