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The map label for this gene is prsA [H]

Identifier: 197119141

GI number: 197119141

Start: 3195422

End: 3196372

Strand: Reverse

Name: prsA [H]

Synonym: Gbem_2768

Alternate gene names: 197119141

Gene position: 3196372-3195422 (Counterclockwise)

Preceding gene: 197119143

Following gene: 197119140

Centisome position: 69.26

GC content: 60.36

Gene sequence:

>951_bases
ATGGAAAACAAGATCAGGGTGTTCAGCGGTAACTCCAATCCTATTCTGGCAGAAAAGATCTGTGATTGCCTGAAGGTGCC
CCTGGGGAAGGCAAAGGTCAAGACCTTCTCCGACGGCGAGATCATGGTCGAGATCGGCGAGAACGTCCGCGGGCGTGACA
TCTACGTGGTGCAGTCGACCTGCTGTCCGACCAACAACAATCTGATGGAACTGCTGATCATGATCGACGCCCTGAAGAGG
GCTTCGGCGGCTACCATCACCGCCGTTATCCCCTACTACGGCTATGCGCGCCAGGACCGCAAGGCCGCACCGAGGACCCC
GATCACTTCCAAGCTCGTGGCCGACCTGATCACTACTGCCGGCGCCGCCAGGGTGGTGACCGTGGACCTTCACGCGGGGC
AGATCCAGGGCTTTTTCAATATCCCTGTGGACAACCTCTACGCCGCTCCGGTTTTGCTCGCCCACCTGAAGAGCCGCTTC
GCCGACGATCCGGACAACCTGGTCATGGTGTCGCCGGACGCAGGCGGGACCGAACGCGCCAGGGCCTTCGCCAAGAGGCT
CGGCTGCACGCTGGCCGTCATCGACAAGCGCCGTACCGGCCCGAACGTGGCCGAGATCATGCACCTGATCGGCGACGTGA
AAGGTAAAAACGCCATCATCCTGGACGACATGATCGACACGGCAGGGACCTTGACCCAGGCAGCTCTCGCTCTGAAACAG
CACGGCGCCGCCAACGTCTACGCCTGCGCCACACATGGCGTGCTTTCCGGCCCCGCGATCGACAGGATCAACGCCTCGGT
CATCGAGAAAGTCGTCATCACCGATACGGTTCCCCTGGGCGAGAAAGCGGAGCTTTCGGACAAGATCAGGGTGCTTTCCG
TTGCAGAACTTTTGGGAGAAGCGATCCGCCGCATCCATGAGGATGAATCGGTCAGCTCGCTTTTCGTGTAA

Upstream 100 bases:

>100_bases
AGTAGCGTTTCCTGGATTTGTGATGCCGGTCTAGGTGGGGTTGGGAAGCCTGCAGGCCGCGCAGAACCTAAATAAAAGAA
GTTACGAGGGCAGAGCTTCA

Downstream 100 bases:

>100_bases
TTGAAATTCAAAAAAACGTCATTGGGTCATTAAATACCAAGATCGACATCGGAGGATGTATGAGTAAGCAGGTGCTGAAA
GCTGAACTGAGAGAGCAAAC

Product: ribose-5-phosphate 1-pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MENKIRVFSGNSNPILAEKICDCLKVPLGKAKVKTFSDGEIMVEIGENVRGRDIYVVQSTCCPTNNNLMELLIMIDALKR
ASAATITAVIPYYGYARQDRKAAPRTPITSKLVADLITTAGAARVVTVDLHAGQIQGFFNIPVDNLYAAPVLLAHLKSRF
ADDPDNLVMVSPDAGGTERARAFAKRLGCTLAVIDKRRTGPNVAEIMHLIGDVKGKNAIILDDMIDTAGTLTQAALALKQ
HGAANVYACATHGVLSGPAIDRINASVIEKVVITDTVPLGEKAELSDKIRVLSVAELLGEAIRRIHEDESVSSLFV

Sequences:

>Translated_316_residues
MENKIRVFSGNSNPILAEKICDCLKVPLGKAKVKTFSDGEIMVEIGENVRGRDIYVVQSTCCPTNNNLMELLIMIDALKR
ASAATITAVIPYYGYARQDRKAAPRTPITSKLVADLITTAGAARVVTVDLHAGQIQGFFNIPVDNLYAAPVLLAHLKSRF
ADDPDNLVMVSPDAGGTERARAFAKRLGCTLAVIDKRRTGPNVAEIMHLIGDVKGKNAIILDDMIDTAGTLTQAALALKQ
HGAANVYACATHGVLSGPAIDRINASVIEKVVITDTVPLGEKAELSDKIRVLSVAELLGEAIRRIHEDESVSSLFV
>Mature_316_residues
MENKIRVFSGNSNPILAEKICDCLKVPLGKAKVKTFSDGEIMVEIGENVRGRDIYVVQSTCCPTNNNLMELLIMIDALKR
ASAATITAVIPYYGYARQDRKAAPRTPITSKLVADLITTAGAARVVTVDLHAGQIQGFFNIPVDNLYAAPVLLAHLKSRF
ADDPDNLVMVSPDAGGTERARAFAKRLGCTLAVIDKRRTGPNVAEIMHLIGDVKGKNAIILDDMIDTAGTLTQAALALKQ
HGAANVYACATHGVLSGPAIDRINASVIEKVVITDTVPLGEKAELSDKIRVLSVAELLGEAIRRIHEDESVSSLFV

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=311, Percent_Identity=52.0900321543408, Blast_Score=318, Evalue=3e-87,
Organism=Homo sapiens, GI4506129, Length=311, Percent_Identity=51.1254019292604, Blast_Score=315, Evalue=3e-86,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=50.6369426751592, Blast_Score=310, Evalue=9e-85,
Organism=Homo sapiens, GI28557709, Length=311, Percent_Identity=50.8038585209003, Blast_Score=309, Evalue=2e-84,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=38.7755102040816, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=39.5348837209302, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI310128524, Length=147, Percent_Identity=34.0136054421769, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI310115209, Length=147, Percent_Identity=34.0136054421769, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI310118259, Length=147, Percent_Identity=34.0136054421769, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI310119946, Length=147, Percent_Identity=34.0136054421769, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1787458, Length=313, Percent_Identity=53.3546325878594, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI25149168, Length=311, Percent_Identity=50.4823151125402, Blast_Score=311, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17554702, Length=311, Percent_Identity=50.4823151125402, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI71989924, Length=311, Percent_Identity=50.4823151125402, Blast_Score=310, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI17554704, Length=309, Percent_Identity=50.4854368932039, Blast_Score=309, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17570245, Length=336, Percent_Identity=38.0952380952381, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319403, Length=315, Percent_Identity=47.6190476190476, Blast_Score=283, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=46.3492063492063, Blast_Score=275, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=46.8354430379747, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=198, Percent_Identity=41.9191919191919, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=109, Percent_Identity=48.6238532110092, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21355239, Length=311, Percent_Identity=52.0900321543408, Blast_Score=310, Evalue=6e-85,
Organism=Drosophila melanogaster, GI45551540, Length=334, Percent_Identity=48.502994011976, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI281362873, Length=354, Percent_Identity=37.0056497175141, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24651454, Length=354, Percent_Identity=37.0056497175141, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=37.0056497175141, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=37.0056497175141, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24651462, Length=373, Percent_Identity=35.1206434316354, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24651464, Length=373, Percent_Identity=35.1206434316354, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI45552010, Length=373, Percent_Identity=35.1206434316354, Blast_Score=207, Evalue=8e-54,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33898; Mature: 33898

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKIRVFSGNSNPILAEKICDCLKVPLGKAKVKTFSDGEIMVEIGENVRGRDIYVVQST
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCEEEEEEEC
CCPTNNNLMELLIMIDALKRASAATITAVIPYYGYARQDRKAAPRTPITSKLVADLITTA
CCCCCCHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
GAARVVTVDLHAGQIQGFFNIPVDNLYAAPVLLAHLKSRFADDPDNLVMVSPDAGGTERA
CCCEEEEEEEECCEECEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
RAFAKRLGCTLAVIDKRRTGPNVAEIMHLIGDVKGKNAIILDDMIDTAGTLTQAALALKQ
HHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
HGAANVYACATHGVLSGPAIDRINASVIEKVVITDTVPLGEKAELSDKIRVLSVAELLGE
CCCCCEEEEECCCCCCCCCHHHCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHH
AIRRIHEDESVSSLFV
HHHHHCCCCCHHHHCC
>Mature Secondary Structure
MENKIRVFSGNSNPILAEKICDCLKVPLGKAKVKTFSDGEIMVEIGENVRGRDIYVVQST
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCEEEEEEEC
CCPTNNNLMELLIMIDALKRASAATITAVIPYYGYARQDRKAAPRTPITSKLVADLITTA
CCCCCCHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
GAARVVTVDLHAGQIQGFFNIPVDNLYAAPVLLAHLKSRFADDPDNLVMVSPDAGGTERA
CCCEEEEEEEECCEECEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
RAFAKRLGCTLAVIDKRRTGPNVAEIMHLIGDVKGKNAIILDDMIDTAGTLTQAALALKQ
HHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
HGAANVYACATHGVLSGPAIDRINASVIEKVVITDTVPLGEKAELSDKIRVLSVAELLGE
CCCCCEEEEECCCCCCCCCHHHCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHH
AIRRIHEDESVSSLFV
HHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]