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The map label for this gene is ribF [H]

Identifier: 197118976

GI number: 197118976

Start: 3010959

End: 3011930

Strand: Reverse

Name: ribF [H]

Synonym: Gbem_2599

Alternate gene names: 197118976

Gene position: 3011930-3010959 (Counterclockwise)

Preceding gene: 197118977

Following gene: 197118975

Centisome position: 65.26

GC content: 63.68

Gene sequence:

>972_bases
ATGGTTATTTTCAGAAGCATCTCCGACATAAAGGAAAAACTGCGCCACCCGGCGGTCACCATCGGCAACTTCGACGGAGT
TCATCTGGGGCACCGCGAGATCTTCCGGAGGGTGCGCGAACGGGCTGCAGAACTTTCCGGGGTCTCGGTGGTGGTGACCT
TCGTGCCGCACCCGCTGAAGGTGCTCGGCGCCCACAAGGGGATCCGGCTCATCACCACACGCCGCGAGAAGGAGTTCCTC
ATCGAGTCAGCCGGGATCGACTACCTCTTGGAGATCCCCTTCGACCGGGAGTTCGCCGCGGTTCCCGCCGCCGATTTCGT
GCAAAAGGTGCTGGTGGACGCCATCGGGGTCGAAAGCCTCGTCATCGGCTACGATTACGCCTTTGGCCGCGGCAGGGAAG
GGAACGTGCAGTTGCTGCGCGGGATGGGCGAGCGTTACGGCTACAGCGTCGAGGTGCTGGACCCCATCTCCAACGGCGCC
ACGGTCTACAGCTCAACCAAGGTCCGCGACATGGTGAGCAAGGGCGACGTGGAAGGGGTGGTCGGCGTCCTGGGGCGGCA
TTACGCCATCACCGGCACCGTGGTGCACGGCCACCAGCGCGGCCGGGGGCTAGGCTTTCCCACCGCGAACCTCGCCATGG
AGAAGGACCTCCTTCCGGCGGCGGGAGTCTACGCGGTCAAGGTCTGCATCGGCAGCGACCTTTTGGATGGCGCCTGCAAC
ATAGGAACGAACCCCACCTTCGGCAACGGGGCGCTTTCGCTGGAGGTATTCCTCTTCGACCTCGACCGCGACCTCTACGG
TCTGGAGGTCACCGTGTTCTTCATCGAGAGGCTCAGGGAGGAGCGCCGTTTCGACGACCTTCAGGCGCTCAAGGAACAGA
TCGCGCTGGACGTTTCGCGCTGCCGGGGCATCTTGCATGATGCAGAACCGGTCGGGGCCGGCGACTGGGGCCCCCTTAAG
TGCTGCAAGTAA

Upstream 100 bases:

>100_bases
CATGTACGAGGTCAGCGTCTGGGTGGCCCTTCTCTTCGGGAAGAAGAAGGCGCCGCAGGTGGAGGCGGAACCGGCTGAGG
CTTAAGGAAACGCACCGCAC

Downstream 100 bases:

>100_bases
CCCATCACCGTCGCAGACAGGAGCCAGCTTGTTCAAGGAAAAACTAGATAAGAAACTCTTGGTCGGCCTCGCGATCAGCG
CGCTTTGCCTGTTGTTGCTC

Product: bifunctional riboflavin kinase/FAD synthetase

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MVIFRSISDIKEKLRHPAVTIGNFDGVHLGHREIFRRVRERAAELSGVSVVVTFVPHPLKVLGAHKGIRLITTRREKEFL
IESAGIDYLLEIPFDREFAAVPAADFVQKVLVDAIGVESLVIGYDYAFGRGREGNVQLLRGMGERYGYSVEVLDPISNGA
TVYSSTKVRDMVSKGDVEGVVGVLGRHYAITGTVVHGHQRGRGLGFPTANLAMEKDLLPAAGVYAVKVCIGSDLLDGACN
IGTNPTFGNGALSLEVFLFDLDRDLYGLEVTVFFIERLREERRFDDLQALKEQIALDVSRCRGILHDAEPVGAGDWGPLK
CCK

Sequences:

>Translated_323_residues
MVIFRSISDIKEKLRHPAVTIGNFDGVHLGHREIFRRVRERAAELSGVSVVVTFVPHPLKVLGAHKGIRLITTRREKEFL
IESAGIDYLLEIPFDREFAAVPAADFVQKVLVDAIGVESLVIGYDYAFGRGREGNVQLLRGMGERYGYSVEVLDPISNGA
TVYSSTKVRDMVSKGDVEGVVGVLGRHYAITGTVVHGHQRGRGLGFPTANLAMEKDLLPAAGVYAVKVCIGSDLLDGACN
IGTNPTFGNGALSLEVFLFDLDRDLYGLEVTVFFIERLREERRFDDLQALKEQIALDVSRCRGILHDAEPVGAGDWGPLK
CCK
>Mature_323_residues
MVIFRSISDIKEKLRHPAVTIGNFDGVHLGHREIFRRVRERAAELSGVSVVVTFVPHPLKVLGAHKGIRLITTRREKEFL
IESAGIDYLLEIPFDREFAAVPAADFVQKVLVDAIGVESLVIGYDYAFGRGREGNVQLLRGMGERYGYSVEVLDPISNGA
TVYSSTKVRDMVSKGDVEGVVGVLGRHYAITGTVVHGHQRGRGLGFPTANLAMEKDLLPAAGVYAVKVCIGSDLLDGACN
IGTNPTFGNGALSLEVFLFDLDRDLYGLEVTVFFIERLREERRFDDLQALKEQIALDVSRCRGILHDAEPVGAGDWGPLK
CCK

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=312, Percent_Identity=37.8205128205128, Blast_Score=204, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 35440; Mature: 35440

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00878 ODR_DC_2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIFRSISDIKEKLRHPAVTIGNFDGVHLGHREIFRRVRERAAELSGVSVVVTFVPHPLK
CEEECCHHHHHHHHCCCEEEECCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
VLGAHKGIRLITTRREKEFLIESAGIDYLLEIPFDREFAAVPAADFVQKVLVDAIGVESL
HHCCCCCEEEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCHHHE
VIGYDYAFGRGREGNVQLLRGMGERYGYSVEVLDPISNGATVYSSTKVRDMVSKGDVEGV
EEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCEEEEECHHHHHHHHCCCHHHH
VGVLGRHYAITGTVVHGHQRGRGLGFPTANLAMEKDLLPAAGVYAVKVCIGSDLLDGACN
HHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCHHEEEEECCCCHHCCCCC
IGTNPTFGNGALSLEVFLFDLDRDLYGLEVTVFFIERLREERRFDDLQALKEQIALDVSR
CCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
CRGILHDAEPVGAGDWGPLKCCK
HHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVIFRSISDIKEKLRHPAVTIGNFDGVHLGHREIFRRVRERAAELSGVSVVVTFVPHPLK
CEEECCHHHHHHHHCCCEEEECCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
VLGAHKGIRLITTRREKEFLIESAGIDYLLEIPFDREFAAVPAADFVQKVLVDAIGVESL
HHCCCCCEEEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCHHHE
VIGYDYAFGRGREGNVQLLRGMGERYGYSVEVLDPISNGATVYSSTKVRDMVSKGDVEGV
EEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCEEEEECHHHHHHHHCCCHHHH
VGVLGRHYAITGTVVHGHQRGRGLGFPTANLAMEKDLLPAAGVYAVKVCIGSDLLDGACN
HHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCHHEEEEECCCCHHCCCCC
IGTNPTFGNGALSLEVFLFDLDRDLYGLEVTVFFIERLREERRFDDLQALKEQIALDVSR
CCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
CRGILHDAEPVGAGDWGPLKCCK
HHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]