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The map label for this gene is azoR

Identifier: 197118906

GI number: 197118906

Start: 2931833

End: 2932465

Strand: Direct

Name: azoR

Synonym: Gbem_2529

Alternate gene names: 197118906

Gene position: 2931833-2932465 (Clockwise)

Preceding gene: 197118905

Following gene: 197118910

Centisome position: 63.53

GC content: 53.08

Gene sequence:

>633_bases
ATGGCAAGACTACTCTACGTTACCTGCAACCTTAAACCTCTGGAGATTTCCCGGAGCCTCTCGGTCGGGAACGAATTCCT
GCATGAATACCTCAAACAAAATCCTGACGACGAGGTGCATTTTCTCGACCTTTACCGCGACCACATCCAGCGGATCGATG
CCGATGTTCTCTCCGGCTGGGGGAAACTGCGGAGTGGCGCGAGCCTTGCGACCCTCTCCGACGACGAGCAGCGCAAAGTC
AGCCGCATCTGGAAGCACGCAGACCAATTCATCGCAGCGGACAAGTACGTGTTCGTGACACCGATGTACAATCTCGGCTT
TCCGGCGGAATTCAAGATGTATATAGATGCGGTATGCGTGGTCGGCAAAACTTTCACATACACCCCTGCCGGCGCTGTCG
GTCTTTTGAAAAACCAGGGCAGAAAGTGTCTCCATATTCACTCGAATGGCGGCTTCCATTACGGCAAGGAAGAGGACCAC
TCCGTCCCTTATCTCAAATCAATTATGGGCTTCATGGGGATCGAAGACTTTGAATCGGTCGTCTTGGAGGGGGTCGACGC
CATGCCGGACTTGGCAGAAGAATTCAAACAAGCAGCCGTAATAAAGGCCCATCTAGCCGCGCAGAGCTTTTAA

Upstream 100 bases:

>100_bases
TTTAAAGACAGCCCAAGGCGGGTGAGGAAGGATAAACAACCAAAGAATATTGGGGAATGCCTCCAATAGACTGTAGAAAA
TCACTAAGAAGGAGAACATT

Downstream 100 bases:

>100_bases
GGGATGAAAGGGGTCGTATTGCTAACCTTGTTCGCCTGGGTCTGCGTCTGTCTGATAGCCTTTTTCCATAAGAGAGAACG
GTGAGGAGCTGGCAGAGCCC

Product: FMN-dependent NADH-azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MARLLYVTCNLKPLEISRSLSVGNEFLHEYLKQNPDDEVHFLDLYRDHIQRIDADVLSGWGKLRSGASLATLSDDEQRKV
SRIWKHADQFIAADKYVFVTPMYNLGFPAEFKMYIDAVCVVGKTFTYTPAGAVGLLKNQGRKCLHIHSNGGFHYGKEEDH
SVPYLKSIMGFMGIEDFESVVLEGVDAMPDLAEEFKQAAVIKAHLAAQSF

Sequences:

>Translated_210_residues
MARLLYVTCNLKPLEISRSLSVGNEFLHEYLKQNPDDEVHFLDLYRDHIQRIDADVLSGWGKLRSGASLATLSDDEQRKV
SRIWKHADQFIAADKYVFVTPMYNLGFPAEFKMYIDAVCVVGKTFTYTPAGAVGLLKNQGRKCLHIHSNGGFHYGKEEDH
SVPYLKSIMGFMGIEDFESVVLEGVDAMPDLAEEFKQAAVIKAHLAAQSF
>Mature_209_residues
ARLLYVTCNLKPLEISRSLSVGNEFLHEYLKQNPDDEVHFLDLYRDHIQRIDADVLSGWGKLRSGASLATLSDDEQRKVS
RIWKHADQFIAADKYVFVTPMYNLGFPAEFKMYIDAVCVVGKTFTYTPAGAVGLLKNQGRKCLHIHSNGGFHYGKEEDHS
VPYLKSIMGFMGIEDFESVVLEGVDAMPDLAEEFKQAAVIKAHLAAQSF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=183, Percent_Identity=31.1475409836066, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_GEOBB (B5EGQ4)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139333.1
- ProteinModelPortal:   B5EGQ4
- SMR:   B5EGQ4
- GeneID:   6782526
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2529
- HOGENOM:   HBG565834
- OMA:   FSGWGKL
- ProtClustDB:   CLSK2324738
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 23634; Mature: 23503

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLLYVTCNLKPLEISRSLSVGNEFLHEYLKQNPDDEVHFLDLYRDHIQRIDADVLSGW
CCEEEEEEECCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GKLRSGASLATLSDDEQRKVSRIWKHADQFIAADKYVFVTPMYNLGFPAEFKMYIDAVCV
HHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHH
VGKTFTYTPAGAVGLLKNQGRKCLHIHSNGGFHYGKEEDHSVPYLKSIMGFMGIEDFESV
HCCCEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHH
VLEGVDAMPDLAEEFKQAAVIKAHLAAQSF
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARLLYVTCNLKPLEISRSLSVGNEFLHEYLKQNPDDEVHFLDLYRDHIQRIDADVLSGW
CEEEEEEECCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GKLRSGASLATLSDDEQRKVSRIWKHADQFIAADKYVFVTPMYNLGFPAEFKMYIDAVCV
HHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHH
VGKTFTYTPAGAVGLLKNQGRKCLHIHSNGGFHYGKEEDHSVPYLKSIMGFMGIEDFESV
HCCCEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHH
VLEGVDAMPDLAEEFKQAAVIKAHLAAQSF
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA