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The map label for this gene is emrA [H]

Identifier: 197118905

GI number: 197118905

Start: 2930688

End: 2931737

Strand: Direct

Name: emrA [H]

Synonym: Gbem_2528

Alternate gene names: 197118905

Gene position: 2930688-2931737 (Clockwise)

Preceding gene: 197118904

Following gene: 197118906

Centisome position: 63.5

GC content: 55.24

Gene sequence:

>1050_bases
ATGGCATTTCCAAAAACAGCAATGATCATCTCCGGCCTCGTTTTAGTGTCCGTAGTCGGAGCGGGTATTTACCTTCTCAA
TCGTCACCAATCCGATGCGGGTATGCAATCAACCGATGATGCATATGTGCGGGCGGATTTTACCTTGGTAGCCCCGCGGG
TAAACGGGCAGATAGCTCGTGTTCTTGTGGATGATAATGAAACCGTAAAGGCTGGTCAGCTGCTGGCTGTAATCGACGAC
CACGATCTTAAGGTTGCCGTACAGAGCGCACAAGCGGAGGCAGGAGCAGCGCAGGCCGCCATTGAAAGCCTGAAGGCAAC
CATCAAGCGAAATGCGTCAACCATTCAGCAGGCTCAGGCTGCCGTAAGGTCGGATGAAGCTTCGATCGCCCTCGCTAAGG
CCAATGCCGAGCGTTATCGGAATTTGTCAACTGATGGTTCAGGAACTCGCCAGGAAGCTCAGAATGCGGATACCCAATTA
CAGGTGCACCTGGCAGCGCTCGACCGCGACAAGGCAGGGGTAGAAGCAGCCCGACAGCAAACCTCGATACTTGAAGCCGA
TCTGAAAAAAGCGGAAAGCTCACTGGAGCGTGCGAAGGCCGTACAGGCTGCCGCCGAGCTCAACCTTTCCTATACCAAGA
TCGTCGCTCCCATAGACGGGACCATAGCACGCCGTTCCGTGCGCCAGGGCGCATTCGTCACCACCGGTATGCCTTTGCTG
GCAGTGGTGCCGCTGGATAAGGTGTATATCGAAGCCAACTTCCGCGAAACACAACTTGCCCGCATTCGCCCGGGACAATC
CGTTACAATCCGTGTTGATACCCTGCCTGGCGTGGTGCTGTCGGGCAGCGTCGCAAGTTTATCGCCGGCAAGCGGTGCTT
CATTTGCCCCTGTAGCTCCGCAGAACGCAACTGGCAATTTCACCAAAATTATTCAGCGCTTGCCGGTTCGCATAAGTATT
ACCCCCGGACAAGTCGCCAGCGGGCAGTTACGAGTTGGCATGTCCGTACAGCCAACGATTGAGATCTGTGAAGGCAAGGG
GAGCGTTTAA

Upstream 100 bases:

>100_bases
GCGCTGGCGGTGCTGCTCATACCTTTCGTATTAAGAATGGATTACATAGCGGCTCCGGCCAGGCAAACCTCAGCACCTAT
CACAAAGAAAGGATAAGACA

Downstream 100 bases:

>100_bases
AGACAGCCCAAGGCGGGTGAGGAAGGATAAACAACCAAAGAATATTGGGGAATGCCTCCAATAGACTGTAGAAAATCACT
AAGAAGGAGAACATTATGGC

Product: RND family efflux pump membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MAFPKTAMIISGLVLVSVVGAGIYLLNRHQSDAGMQSTDDAYVRADFTLVAPRVNGQIARVLVDDNETVKAGQLLAVIDD
HDLKVAVQSAQAEAGAAQAAIESLKATIKRNASTIQQAQAAVRSDEASIALAKANAERYRNLSTDGSGTRQEAQNADTQL
QVHLAALDRDKAGVEAARQQTSILEADLKKAESSLERAKAVQAAAELNLSYTKIVAPIDGTIARRSVRQGAFVTTGMPLL
AVVPLDKVYIEANFRETQLARIRPGQSVTIRVDTLPGVVLSGSVASLSPASGASFAPVAPQNATGNFTKIIQRLPVRISI
TPGQVASGQLRVGMSVQPTIEICEGKGSV

Sequences:

>Translated_349_residues
MAFPKTAMIISGLVLVSVVGAGIYLLNRHQSDAGMQSTDDAYVRADFTLVAPRVNGQIARVLVDDNETVKAGQLLAVIDD
HDLKVAVQSAQAEAGAAQAAIESLKATIKRNASTIQQAQAAVRSDEASIALAKANAERYRNLSTDGSGTRQEAQNADTQL
QVHLAALDRDKAGVEAARQQTSILEADLKKAESSLERAKAVQAAAELNLSYTKIVAPIDGTIARRSVRQGAFVTTGMPLL
AVVPLDKVYIEANFRETQLARIRPGQSVTIRVDTLPGVVLSGSVASLSPASGASFAPVAPQNATGNFTKIIQRLPVRISI
TPGQVASGQLRVGMSVQPTIEICEGKGSV
>Mature_348_residues
AFPKTAMIISGLVLVSVVGAGIYLLNRHQSDAGMQSTDDAYVRADFTLVAPRVNGQIARVLVDDNETVKAGQLLAVIDDH
DLKVAVQSAQAEAGAAQAAIESLKATIKRNASTIQQAQAAVRSDEASIALAKANAERYRNLSTDGSGTRQEAQNADTQLQ
VHLAALDRDKAGVEAARQQTSILEADLKKAESSLERAKAVQAAAELNLSYTKIVAPIDGTIARRSVRQGAFVTTGMPLLA
VVPLDKVYIEANFRETQLARIRPGQSVTIRVDTLPGVVLSGSVASLSPASGASFAPVAPQNATGNFTKIIQRLPVRISIT
PGQVASGQLRVGMSVQPTIEICEGKGSV

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=343, Percent_Identity=31.7784256559767, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788711, Length=312, Percent_Identity=31.4102564102564, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790519, Length=306, Percent_Identity=29.0849673202614, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081951, Length=325, Percent_Identity=29.2307692307692, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789637, Length=335, Percent_Identity=25.9701492537313, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790012, Length=243, Percent_Identity=28.3950617283951, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787013, Length=296, Percent_Identity=30.0675675675676, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790024, Length=250, Percent_Identity=26.4, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36685; Mature: 36554

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFPKTAMIISGLVLVSVVGAGIYLLNRHQSDAGMQSTDDAYVRADFTLVAPRVNGQIAR
CCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEE
VLVDDNETVKAGQLLAVIDDHDLKVAVQSAQAEAGAAQAAIESLKATIKRNASTIQQAQA
EEECCCCEEECCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
AVRSDEASIALAKANAERYRNLSTDGSGTRQEAQNADTQLQVHLAALDRDKAGVEAARQQ
HHHCCCCEEEEEECCHHHHCCCCCCCCCCHHHHHCCCCEEEEEEEEECCCHHHHHHHHHH
TSILEADLKKAESSLERAKAVQAAAELNLSYTKIVAPIDGTIARRSVRQGAFVTTGMPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCEEECCCCEE
AVVPLDKVYIEANFRETQLARIRPGQSVTIRVDTLPGVVLSGSVASLSPASGASFAPVAP
EEECCCEEEEEECCCCCCCEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCC
QNATGNFTKIIQRLPVRISITPGQVASGQLRVGMSVQPTIEICEGKGSV
CCCCCHHHHHHHHCCEEEEECCCCCCCCEEEECCCCCCCEEEECCCCCC
>Mature Secondary Structure 
AFPKTAMIISGLVLVSVVGAGIYLLNRHQSDAGMQSTDDAYVRADFTLVAPRVNGQIAR
CCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEE
VLVDDNETVKAGQLLAVIDDHDLKVAVQSAQAEAGAAQAAIESLKATIKRNASTIQQAQA
EEECCCCEEECCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
AVRSDEASIALAKANAERYRNLSTDGSGTRQEAQNADTQLQVHLAALDRDKAGVEAARQQ
HHHCCCCEEEEEECCHHHHCCCCCCCCCCHHHHHCCCCEEEEEEEEECCCHHHHHHHHHH
TSILEADLKKAESSLERAKAVQAAAELNLSYTKIVAPIDGTIARRSVRQGAFVTTGMPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCEEECCCCEE
AVVPLDKVYIEANFRETQLARIRPGQSVTIRVDTLPGVVLSGSVASLSPASGASFAPVAP
EEECCCEEEEEECCCCCCCEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCC
QNATGNFTKIIQRLPVRISITPGQVASGQLRVGMSVQPTIEICEGKGSV
CCCCCHHHHHHHHCCEEEEECCCCCCCCEEEECCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]