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The map label for this gene is pflD [H]

Identifier: 197118876

GI number: 197118876

Start: 2897430

End: 2899790

Strand: Reverse

Name: pflD [H]

Synonym: Gbem_2499

Alternate gene names: 197118876

Gene position: 2899790-2897430 (Counterclockwise)

Preceding gene: 197118877

Following gene: 197118875

Centisome position: 62.83

GC content: 63.74

Gene sequence:

>2361_bases
ATGACCGGAAGAACTGAACGTCTGCGGCAGGAGAGCCTTGACGCCGAGGCGGCGATTTCGGCGGAGCGCGCGCTGCTCCT
CACCGAGTTTTACCGCGAGAACGAGGGGCGCTGGTCCATCCCGGTGCTGCGGGCCAAGTCCTTTTACTACCTCTGCGAGA
AGAAGACCATCTATCTCGGCGACGGGGAACTGGTGGTGGGGGAGAGGGGGCCTGCTCCCAAGCTGGTTCCGACCTACCCG
GAGCTTACCTGCCACTCGCTTAAGGACCTGCGCATCCTGAACTCGCGCGAGAAGACCAGCTACCGCGTCGACGACGCCTG
CCTCAAGGCTTACGGGGAGACGGTGATCCCCTACTGGAGCACGAGGTCGCTTCGGGACAAGATCTTCCGGGAACTCCCCG
AGGAATGGCACGAGGCCTACCAAAGCGGCATCTTCACCGAGTTCATGGAGCAGCGCGCCCCCGGGCACACGGTGTTGGAC
GACAAGATCTACAATAAGGGGCTCCTCGATTTCAAGAAGGAGATAGCGGACGCCATCGCCGCGCTCGATTTCGTCAGCGA
TCCGAACGCCTGGTCCAAGCGGGAGGAGCTGAAGGCGATGGATATCTCCTGCGACGCCGTCATCCTTTTCGCCGAACGCC
ACGCGAAACTTGCCGAAAGCATGGCCGCGGCGTGCGCCGATGCCGTCCGCAAGCAGGAGCTCTTGAAGATCGCCGCCAAC
TGCCGTTGGGTTCCGGCGCACGCCCCTACCGATTTCTTCGAGGCGCTGCAGTCCTACTGGTTCTGCCACCTGGCGGTCAT
TACGGAGCTGAACGGCTGGGACGCCTTCAGCCCCGGGCACCTCGACCAGCACCTCTACCCTTTCTACCGGGACGGGCTCG
CCGCCGGCACCCTGAAAAAGGAGGAGGCGCGCGAGCTCCTCGAATGCTTCTTCGTCAAGTTCAACAACCACCCCTCGCCC
CCCAAGGTGGGGGTGACCGCGGCCGAGAGCGGCACCTACACCGACTTCGCCAACATCAACCTGGGGGGGCTTCTCCCGGA
CGGCTCCGACGGCTCCAACGAGGTGTCGCATCTGCTTCTGGACATCATCGACGAGATGCACCTGTTGCAGCCTTCCAGCA
ACATCCAGCTCTCCCGCAAGTCCCCCGACCGCTTCCTGAAGCACACCTTGAGGGTGGTGCGCAAGGGGTACGGCTTTCCA
TCCATCTTCAACGCGGACTCCGTGGTCGAGGAGCAGCTGCGCCAGGGAAAGACGCTCGCGGACGCCCGCGCAGGGGGGTG
CAGCGGCTGCGTCGAGGTCGGGGCGTTCGGCAAGGAGGCCTACATCCTCACCGGCTACTTCAACCTGGTGAAGATGCTGG
AACTGGCGCTGCACGACGGCGTCGACCCGGCAACCGGCGCCCAGCTGGGGCCCGCTACCGGTAAAACGGAGAGTTTCGCC
TGCTTCGACGACGTCTTCGCCGCCTTCACCAGGCAGCTCGCCCATTTCATCGATATCAAGATCCGCGGCAACCGCCTCAT
CGAGATGATCTACGCAGGCCTCATGCCCGCTCCGTTTCTGTCGGTTTTGACCGATGACTGCATCAGCCGCGGGGTGGATT
ACAACGCGGGAGGTGCCAGGTACAACAACAGCTTCATCCAGGGGGTGGGGATAGGGAGCATCACCGATTCGCTCTCCGCC
ATCAAGGAGCACGTTTTCCAAGAGAAGGCGGTCTCGCTCCCCGACCTGGTGGCCAAGCTCGATGCGGACTTCGCTTCGGA
CGAGCCGCTCAGGCAGCGGCTTTGGAACCGGACCCGCAAGTACGGCAATGACGACGACTTCGCCGACGACCTGATGCGGC
AGGTCTTCGAGGCCTTCTTCCAGCAGGTGGACGGCAGGCCCAACACGAAGGGGGGCGTGTACCGCATCGAGATGCTCCCG
ACCACCTGCCACGTCTATTTCGGCTCGGTCACCGGGGCAATGCCCGACGGCAGGCGCCGGGGCGCTCCCCTTTCCGAGGG
GATCTCGCCGGTGCAGGGGGCGGACCGCCAGGGACCTACCGCCGTCATCCGTTCGGCCGGGAAGATGGATCACATCCGCA
GCGGCGGCACCCTTTTGAACCTGAAGTTCTCCCCGACGCTTTTAGCCGGGGAGGGGGGCGTCGACGGACTGGCGGCGCTG
GTGCGCAGCTACTTCAGGATGGACGGCCATCACGTCCAGTTCAACGTGGTGGACGTGGAGACGCTGAAAAGGGCGCAGGC
CGATCCCCGGCAGTACCGTGACCTGATTGTCAGGGTGGCCGGCTACAGCGATTACTTCTGCGACCTCTCCGAGGAGCTGC
AAAACGAGATCATAGCCAGGACCGAGCACACGTCCCTGTGA

Upstream 100 bases:

>100_bases
ACCGGCGCAAGAGGCCATGGCGCCCTCGCAGGAGAGTTTGGAACATTATGCCCGACTGTTCAGGGACAAAGGGCTGGATA
CGCGTATAGGGGGAGCTTAG

Downstream 100 bases:

>100_bases
GCGCGGCGACAGCCAAGGAGGGAACATCATGGCCGTAGAACCCGGACGCTACCGGCATTACAAGGGGAATTTCTACCAGG
TCATCGGCACCGCGCGTCAC

Product: glycerol dehydratase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 786; Mature: 785

Protein sequence:

>786_residues
MTGRTERLRQESLDAEAAISAERALLLTEFYRENEGRWSIPVLRAKSFYYLCEKKTIYLGDGELVVGERGPAPKLVPTYP
ELTCHSLKDLRILNSREKTSYRVDDACLKAYGETVIPYWSTRSLRDKIFRELPEEWHEAYQSGIFTEFMEQRAPGHTVLD
DKIYNKGLLDFKKEIADAIAALDFVSDPNAWSKREELKAMDISCDAVILFAERHAKLAESMAAACADAVRKQELLKIAAN
CRWVPAHAPTDFFEALQSYWFCHLAVITELNGWDAFSPGHLDQHLYPFYRDGLAAGTLKKEEARELLECFFVKFNNHPSP
PKVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLLDIIDEMHLLQPSSNIQLSRKSPDRFLKHTLRVVRKGYGFP
SIFNADSVVEEQLRQGKTLADARAGGCSGCVEVGAFGKEAYILTGYFNLVKMLELALHDGVDPATGAQLGPATGKTESFA
CFDDVFAAFTRQLAHFIDIKIRGNRLIEMIYAGLMPAPFLSVLTDDCISRGVDYNAGGARYNNSFIQGVGIGSITDSLSA
IKEHVFQEKAVSLPDLVAKLDADFASDEPLRQRLWNRTRKYGNDDDFADDLMRQVFEAFFQQVDGRPNTKGGVYRIEMLP
TTCHVYFGSVTGAMPDGRRRGAPLSEGISPVQGADRQGPTAVIRSAGKMDHIRSGGTLLNLKFSPTLLAGEGGVDGLAAL
VRSYFRMDGHHVQFNVVDVETLKRAQADPRQYRDLIVRVAGYSDYFCDLSEELQNEIIARTEHTSL

Sequences:

>Translated_786_residues
MTGRTERLRQESLDAEAAISAERALLLTEFYRENEGRWSIPVLRAKSFYYLCEKKTIYLGDGELVVGERGPAPKLVPTYP
ELTCHSLKDLRILNSREKTSYRVDDACLKAYGETVIPYWSTRSLRDKIFRELPEEWHEAYQSGIFTEFMEQRAPGHTVLD
DKIYNKGLLDFKKEIADAIAALDFVSDPNAWSKREELKAMDISCDAVILFAERHAKLAESMAAACADAVRKQELLKIAAN
CRWVPAHAPTDFFEALQSYWFCHLAVITELNGWDAFSPGHLDQHLYPFYRDGLAAGTLKKEEARELLECFFVKFNNHPSP
PKVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLLDIIDEMHLLQPSSNIQLSRKSPDRFLKHTLRVVRKGYGFP
SIFNADSVVEEQLRQGKTLADARAGGCSGCVEVGAFGKEAYILTGYFNLVKMLELALHDGVDPATGAQLGPATGKTESFA
CFDDVFAAFTRQLAHFIDIKIRGNRLIEMIYAGLMPAPFLSVLTDDCISRGVDYNAGGARYNNSFIQGVGIGSITDSLSA
IKEHVFQEKAVSLPDLVAKLDADFASDEPLRQRLWNRTRKYGNDDDFADDLMRQVFEAFFQQVDGRPNTKGGVYRIEMLP
TTCHVYFGSVTGAMPDGRRRGAPLSEGISPVQGADRQGPTAVIRSAGKMDHIRSGGTLLNLKFSPTLLAGEGGVDGLAAL
VRSYFRMDGHHVQFNVVDVETLKRAQADPRQYRDLIVRVAGYSDYFCDLSEELQNEIIARTEHTSL
>Mature_785_residues
TGRTERLRQESLDAEAAISAERALLLTEFYRENEGRWSIPVLRAKSFYYLCEKKTIYLGDGELVVGERGPAPKLVPTYPE
LTCHSLKDLRILNSREKTSYRVDDACLKAYGETVIPYWSTRSLRDKIFRELPEEWHEAYQSGIFTEFMEQRAPGHTVLDD
KIYNKGLLDFKKEIADAIAALDFVSDPNAWSKREELKAMDISCDAVILFAERHAKLAESMAAACADAVRKQELLKIAANC
RWVPAHAPTDFFEALQSYWFCHLAVITELNGWDAFSPGHLDQHLYPFYRDGLAAGTLKKEEARELLECFFVKFNNHPSPP
KVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLLDIIDEMHLLQPSSNIQLSRKSPDRFLKHTLRVVRKGYGFPS
IFNADSVVEEQLRQGKTLADARAGGCSGCVEVGAFGKEAYILTGYFNLVKMLELALHDGVDPATGAQLGPATGKTESFAC
FDDVFAAFTRQLAHFIDIKIRGNRLIEMIYAGLMPAPFLSVLTDDCISRGVDYNAGGARYNNSFIQGVGIGSITDSLSAI
KEHVFQEKAVSLPDLVAKLDADFASDEPLRQRLWNRTRKYGNDDDFADDLMRQVFEAFFQQVDGRPNTKGGVYRIEMLPT
TCHVYFGSVTGAMPDGRRRGAPLSEGISPVQGADRQGPTAVIRSAGKMDHIRSGGTLLNLKFSPTLLAGEGGVDGLAALV
RSYFRMDGHHVQFNVVDVETLKRAQADPRQYRDLIVRVAGYSDYFCDLSEELQNEIIARTEHTSL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=796, Percent_Identity=34.6733668341709, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787044, Length=799, Percent_Identity=32.1652065081352, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI48994926, Length=551, Percent_Identity=27.2232304900181, Blast_Score=139, Evalue=8e-34,
Organism=Escherichia coli, GI1787131, Length=579, Percent_Identity=25.5613126079447, Blast_Score=129, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 87477; Mature: 87346

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRTERLRQESLDAEAAISAERALLLTEFYRENEGRWSIPVLRAKSFYYLCEKKTIYLG
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCEEEEC
DGELVVGERGPAPKLVPTYPELTCHSLKDLRILNSREKTSYRVDDACLKAYGETVIPYWS
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCC
TRSLRDKIFRELPEEWHEAYQSGIFTEFMEQRAPGHTVLDDKIYNKGLLDFKKEIADAIA
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEECHHHHCCCHHHHHHHHHHHHH
ALDFVSDPNAWSKREELKAMDISCDAVILFAERHAKLAESMAAACADAVRKQELLKIAAN
HHHHCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
CRWVPAHAPTDFFEALQSYWFCHLAVITELNGWDAFSPGHLDQHLYPFYRDGLAAGTLKK
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHHHCCCHHCCCCH
EEARELLECFFVKFNNHPSPPKVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLL
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHH
DIIDEMHLLQPSSNIQLSRKSPDRFLKHTLRVVRKGYGFPSIFNADSVVEEQLRQGKTLA
HHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHH
DARAGGCSGCVEVGAFGKEAYILTGYFNLVKMLELALHDGVDPATGAQLGPATGKTESFA
HHCCCCCHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
CFDDVFAAFTRQLAHFIDIKIRGNRLIEMIYAGLMPAPFLSVLTDDCISRGVDYNAGGAR
HHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
YNNSFIQGVGIGSITDSLSAIKEHVFQEKAVSLPDLVAKLDADFASDEPLRQRLWNRTRK
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHC
YGNDDDFADDLMRQVFEAFFQQVDGRPNTKGGVYRIEMLPTTCHVYFGSVTGAMPDGRRR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCC
GAPLSEGISPVQGADRQGPTAVIRSAGKMDHIRSGGTLLNLKFSPTLLAGEGGVDGLAAL
CCCHHHCCCCCCCCCCCCCHHHHHCCCCHHHHCCCCEEEEEEECCEEEECCCCHHHHHHH
VRSYFRMDGHHVQFNVVDVETLKRAQADPRQYRDLIVRVAGYSDYFCDLSEELQNEIIAR
HHHHHHCCCCEEEEEEEEHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHC
TEHTSL
CCCCCC
>Mature Secondary Structure 
TGRTERLRQESLDAEAAISAERALLLTEFYRENEGRWSIPVLRAKSFYYLCEKKTIYLG
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCEEEEC
DGELVVGERGPAPKLVPTYPELTCHSLKDLRILNSREKTSYRVDDACLKAYGETVIPYWS
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCC
TRSLRDKIFRELPEEWHEAYQSGIFTEFMEQRAPGHTVLDDKIYNKGLLDFKKEIADAIA
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEECHHHHCCCHHHHHHHHHHHHH
ALDFVSDPNAWSKREELKAMDISCDAVILFAERHAKLAESMAAACADAVRKQELLKIAAN
HHHHCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
CRWVPAHAPTDFFEALQSYWFCHLAVITELNGWDAFSPGHLDQHLYPFYRDGLAAGTLKK
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHHHCCCHHCCCCH
EEARELLECFFVKFNNHPSPPKVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLL
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHH
DIIDEMHLLQPSSNIQLSRKSPDRFLKHTLRVVRKGYGFPSIFNADSVVEEQLRQGKTLA
HHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHH
DARAGGCSGCVEVGAFGKEAYILTGYFNLVKMLELALHDGVDPATGAQLGPATGKTESFA
HHCCCCCHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
CFDDVFAAFTRQLAHFIDIKIRGNRLIEMIYAGLMPAPFLSVLTDDCISRGVDYNAGGAR
HHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
YNNSFIQGVGIGSITDSLSAIKEHVFQEKAVSLPDLVAKLDADFASDEPLRQRLWNRTRK
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHC
YGNDDDFADDLMRQVFEAFFQQVDGRPNTKGGVYRIEMLPTTCHVYFGSVTGAMPDGRRR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCC
GAPLSEGISPVQGADRQGPTAVIRSAGKMDHIRSGGTLLNLKFSPTLLAGEGGVDGLAAL
CCCHHHCCCCCCCCCCCCCHHHHHCCCCHHHHCCCCEEEEEEECCEEEECCCCHHHHHHH
VRSYFRMDGHHVQFNVVDVETLKRAQADPRQYRDLIVRVAGYSDYFCDLSEELQNEIIAR
HHHHHHCCCCEEEEEEEEHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHC
TEHTSL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]