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The map label for this gene is gph [H]

Identifier: 197118828

GI number: 197118828

Start: 2841644

End: 2842282

Strand: Reverse

Name: gph [H]

Synonym: Gbem_2451

Alternate gene names: 197118828

Gene position: 2842282-2841644 (Counterclockwise)

Preceding gene: 197118829

Following gene: 197118827

Centisome position: 61.59

GC content: 61.82

Gene sequence:

>639_bases
ATGCCCGCGGAGAATATCTTGAGAAAAGAAACCAAGGCGGTCATTTACGACTGCGACGGGGTGCTGTTCGACTCCTTCGA
GGCGAACTACGCCTTCTACGGGCAGGTGGTAGAGCACTTCGGCAAGGCCCCCCTGGACCGCGGCGACGCCGAGCTGATGC
GGATTCTGCACACCTACTGCAACAAGGACGTGCTGTCCCACATCTTCGCCGGAGAAGAGCGGATGGGCGAGGTGCGGGCT
TTCTCGGCCAGTATCGACTACAGGAAGCTTTTCCCCCTGATGGTGATGGAGGAGGGGCTGCGCGAGACACTGGAGGCGCT
GAAGGGGAGGGTGGAGCTTGCCGTCTGCACCAACAGGGCAAGCTCCATGGACATGCTGCTGGAAAGCTTCGGGCTTGCTC
CCTATTTCAGCTGTGTCATGACCGCCGCGCAGGTGCAAAACCCGAAACCACACCCGGAGCCGCTTTGGAAGGTGCTGGCT
CAGTTCGGGATCGCCCCTGAAGAGGCGCTCTTTGTCGGGGATTCCGAGGTGGACCGCCAGGCAGCCGACGCTGCCGGCGT
CCCGTTTATCGCCTACCGCGGCGGGATGCGCGCGCTGGCCAGGATAGAGAAGCACCAGGAACTGCTGTTACTGCTGTAG

Upstream 100 bases:

>100_bases
CTTGCAAGGAATGCAACAACAAGAAGAAGCACCTGCTCCCCATCGAGTGGGAGGAATACCTGGAGACGCTTGCCGGGAAT
AAGCAGCAGTGAAACGGGCC

Downstream 100 bases:

>100_bases
TTGCCGGAGGTGGGACCGATGGAGCTTATGCCGCAGGATGATCAGGGGAGACTTTGCCAGCGTGCGCTGGAGGCGCTGTC
GTCGGGGGACCCCCAGGCAG

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MPAENILRKETKAVIYDCDGVLFDSFEANYAFYGQVVEHFGKAPLDRGDAELMRILHTYCNKDVLSHIFAGEERMGEVRA
FSASIDYRKLFPLMVMEEGLRETLEALKGRVELAVCTNRASSMDMLLESFGLAPYFSCVMTAAQVQNPKPHPEPLWKVLA
QFGIAPEEALFVGDSEVDRQAADAAGVPFIAYRGGMRALARIEKHQELLLLL

Sequences:

>Translated_212_residues
MPAENILRKETKAVIYDCDGVLFDSFEANYAFYGQVVEHFGKAPLDRGDAELMRILHTYCNKDVLSHIFAGEERMGEVRA
FSASIDYRKLFPLMVMEEGLRETLEALKGRVELAVCTNRASSMDMLLESFGLAPYFSCVMTAAQVQNPKPHPEPLWKVLA
QFGIAPEEALFVGDSEVDRQAADAAGVPFIAYRGGMRALARIEKHQELLLLL
>Mature_211_residues
PAENILRKETKAVIYDCDGVLFDSFEANYAFYGQVVEHFGKAPLDRGDAELMRILHTYCNKDVLSHIFAGEERMGEVRAF
SASIDYRKLFPLMVMEEGLRETLEALKGRVELAVCTNRASSMDMLLESFGLAPYFSCVMTAAQVQNPKPHPEPLWKVLAQ
FGIAPEEALFVGDSEVDRQAADAAGVPFIAYRGGMRALARIEKHQELLLLL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=119, Percent_Identity=43.6974789915966, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23708; Mature: 23576

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAENILRKETKAVIYDCDGVLFDSFEANYAFYGQVVEHFGKAPLDRGDAELMRILHTYC
CCHHHHHHHHHHEEEEECCCCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
NKDVLSHIFAGEERMGEVRAFSASIDYRKLFPLMVMEEGLRETLEALKGRVELAVCTNRA
CHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCH
SSMDMLLESFGLAPYFSCVMTAAQVQNPKPHPEPLWKVLAQFGIAPEEALFVGDSEVDRQ
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHH
AADAAGVPFIAYRGGMRALARIEKHQELLLLL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAENILRKETKAVIYDCDGVLFDSFEANYAFYGQVVEHFGKAPLDRGDAELMRILHTYC
CHHHHHHHHHHEEEEECCCCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
NKDVLSHIFAGEERMGEVRAFSASIDYRKLFPLMVMEEGLRETLEALKGRVELAVCTNRA
CHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCH
SSMDMLLESFGLAPYFSCVMTAAQVQNPKPHPEPLWKVLAQFGIAPEEALFVGDSEVDRQ
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHH
AADAAGVPFIAYRGGMRALARIEKHQELLLLL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]