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The map label for this gene is ppnK

Identifier: 197118822

GI number: 197118822

Start: 2834740

End: 2835606

Strand: Reverse

Name: ppnK

Synonym: Gbem_2445

Alternate gene names: 197118822

Gene position: 2835606-2834740 (Counterclockwise)

Preceding gene: 197118826

Following gene: 197118821

Centisome position: 61.44

GC content: 63.32

Gene sequence:

>867_bases
ATGAAAAAAATCGCCATTTTTGCCAAAGTCCACGACCCGCGTGCGCTGGCTGTAGCGGAGGAGCTGATCGAGTGGCTGGC
GGCACGCGGCGTGACCGCGCACGTCGAGGAGCATCTTTCGAAAAGGCTCAGGCGCACCACCCTGGCCGAGAGTTCTGAGA
GTACGGAAATCGCGGCCGACGCCGATCTGGTGGTGGTTTTGGGCGGCGACGGCACCCTCATCGCCGCGGCGAGGCTCGTC
GGCGAGCGGGACGTCCCCATCCTGGCGGTGAACCTCGGGAGCCTCGGTTTTCTCACCGAGATCACCCTGAACGAACTCTA
CCCCTCGGTGGAGCGCTGCCTGGCAGGCGACTTCGAGGTGAGCGAGCGGATGATGCTGATGGCGAGCGTGGAGCGCTCCG
GGGAAGTCGTGGAACTGCACCGGGTGCTGAACGATGTGGTGATCAACAAGGGGGCCTTGGCGAGGATCATCGACATGGAG
ACCTCGGTTAACGGCCGCTACCTGACCACCTTCAAGGCCGACGGTCTGATCGTCTCCACACCGACCGGCTCCACCGGCTA
TTCGCTCTCGGCGAACGGCCCCATCCTGCACCCGGAACTCGAGTGCATCTCGCTCACCCCCATTTGCCCGCATACCCTCA
CCAACCGCCCGCTGGTTATGGCCGCGGACGCGCACATCGCCATCAAGCTTAAGTACGCACCGGACGAATCGGTCTTTCTC
ACCCTCGACGGGCAGGTGGGGATGAAGCTCCTCTCAGGCGACGTGGTGCAGATCACCAAGGCGGCGCATGTGACCCGCCT
GATCCAGTCCCGCAGCAAGGACTACTTCGAGGTCCTGAGGACCAAGCTCAAATGGGGCGAGAGGTGA

Upstream 100 bases:

>100_bases
TGGTGTTTGTAGCTGTGTCTTTCCGTGCCAGTTTCCGTTGGACATGATACTACATCTGTGCTAGTAAATCCTAAATTACA
TTACAAGTAAAGGCCTCCTG

Downstream 100 bases:

>100_bases
GGCGTTGCTGAGAGAACTGCAGATAACGAACCTGGCCATTATCGAGAAGCTGCACGTCGAGTTCGCCCCGGGGCTAAACA
TCCTCACCGGCGAGACCGGC

Product: ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKKIAIFAKVHDPRALAVAEELIEWLAARGVTAHVEEHLSKRLRRTTLAESSESTEIAADADLVVVLGGDGTLIAAARLV
GERDVPILAVNLGSLGFLTEITLNELYPSVERCLAGDFEVSERMMLMASVERSGEVVELHRVLNDVVINKGALARIIDME
TSVNGRYLTTFKADGLIVSTPTGSTGYSLSANGPILHPELECISLTPICPHTLTNRPLVMAADAHIAIKLKYAPDESVFL
TLDGQVGMKLLSGDVVQITKAAHVTRLIQSRSKDYFEVLRTKLKWGER

Sequences:

>Translated_288_residues
MKKIAIFAKVHDPRALAVAEELIEWLAARGVTAHVEEHLSKRLRRTTLAESSESTEIAADADLVVVLGGDGTLIAAARLV
GERDVPILAVNLGSLGFLTEITLNELYPSVERCLAGDFEVSERMMLMASVERSGEVVELHRVLNDVVINKGALARIIDME
TSVNGRYLTTFKADGLIVSTPTGSTGYSLSANGPILHPELECISLTPICPHTLTNRPLVMAADAHIAIKLKYAPDESVFL
TLDGQVGMKLLSGDVVQITKAAHVTRLIQSRSKDYFEVLRTKLKWGER
>Mature_288_residues
MKKIAIFAKVHDPRALAVAEELIEWLAARGVTAHVEEHLSKRLRRTTLAESSESTEIAADADLVVVLGGDGTLIAAARLV
GERDVPILAVNLGSLGFLTEITLNELYPSVERCLAGDFEVSERMMLMASVERSGEVVELHRVLNDVVINKGALARIIDME
TSVNGRYLTTFKADGLIVSTPTGSTGYSLSANGPILHPELECISLTPICPHTLTNRPLVMAADAHIAIKLKYAPDESVFL
TLDGQVGMKLLSGDVVQITKAAHVTRLIQSRSKDYFEVLRTKLKWGER

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=326, Percent_Identity=28.5276073619632, Blast_Score=127, Evalue=8e-30,
Organism=Escherichia coli, GI1788968, Length=288, Percent_Identity=33.6805555555556, Blast_Score=181, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI71999610, Length=195, Percent_Identity=28.2051282051282, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320794, Length=320, Percent_Identity=27.5, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322509, Length=320, Percent_Identity=27.8125, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6325068, Length=239, Percent_Identity=31.7991631799163, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI28573826, Length=317, Percent_Identity=29.6529968454259, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28573832, Length=317, Percent_Identity=29.6529968454259, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28573830, Length=317, Percent_Identity=29.6529968454259, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28573828, Length=317, Percent_Identity=29.6529968454259, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI161077047, Length=317, Percent_Identity=29.6529968454259, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI20129957, Length=272, Percent_Identity=31.9852941176471, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24653422, Length=272, Percent_Identity=31.9852941176471, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI281363321, Length=272, Percent_Identity=31.9852941176471, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281363323, Length=272, Percent_Identity=31.9852941176471, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24653424, Length=272, Percent_Identity=31.9852941176471, Blast_Score=126, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_GEOBB (B5EFY8)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139249.1
- ProteinModelPortal:   B5EFY8
- SMR:   B5EFY8
- GeneID:   6782442
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2445
- HOGENOM:   HBG713904
- OMA:   DGTMLNA
- ProtClustDB:   CLSK828714
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 31350; Mature: 31350

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIFAKVHDPRALAVAEELIEWLAARGVTAHVEEHLSKRLRRTTLAESSESTEIAAD
CCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECC
ADLVVVLGGDGTLIAAARLVGERDVPILAVNLGSLGFLTEITLNELYPSVERCLAGDFEV
CCEEEEECCCCCEEHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCH
SERMMLMASVERSGEVVELHRVLNDVVINKGALARIIDMETSVNGRYLTTFKADGLIVST
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCEEEEC
PTGSTGYSLSANGPILHPELECISLTPICPHTLTNRPLVMAADAHIAIKLKYAPDESVFL
CCCCCCEEEECCCCEECCCCEEEEECCCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEE
TLDGQVGMKLLSGDVVQITKAAHVTRLIQSRSKDYFEVLRTKLKWGER
EECCCCCHHHHCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKIAIFAKVHDPRALAVAEELIEWLAARGVTAHVEEHLSKRLRRTTLAESSESTEIAAD
CCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECC
ADLVVVLGGDGTLIAAARLVGERDVPILAVNLGSLGFLTEITLNELYPSVERCLAGDFEV
CCEEEEECCCCCEEHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCH
SERMMLMASVERSGEVVELHRVLNDVVINKGALARIIDMETSVNGRYLTTFKADGLIVST
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCEEEEC
PTGSTGYSLSANGPILHPELECISLTPICPHTLTNRPLVMAADAHIAIKLKYAPDESVFL
CCCCCCEEEECCCCEECCCCEEEEECCCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEE
TLDGQVGMKLLSGDVVQITKAAHVTRLIQSRSKDYFEVLRTKLKWGER
EECCCCCHHHHCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA