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The map label for this gene is tgt-2 [H]

Identifier: 197118812

GI number: 197118812

Start: 2822966

End: 2824084

Strand: Reverse

Name: tgt-2 [H]

Synonym: Gbem_2435

Alternate gene names: 197118812

Gene position: 2824084-2822966 (Counterclockwise)

Preceding gene: 197118813

Following gene: 197118811

Centisome position: 61.19

GC content: 62.56

Gene sequence:

>1119_bases
TTGGCAGCTATCAGCTTCGAACTCATAAAGAAGGACCCGGGCTCTCTGGCCCGCCTAGGCTCTCTGACAACAACGCACGG
CAGCATCCAGACCCCGATCTTCATGCCGGTCGGCACGCAGGCGACGGTAAAGGCGATGACGCCCGAGGAACTGGTCGAGG
TAGGCTCCCAGATCATCCTCGCCAACACCTACCATCTGTACCTGCGCCCCGGCCATGAACTGATCGCGCGCCTGGGCGGC
CTGCACCGCTTCATGCACTGGGACCGTCCTATGCTCACCGACTCCGGCGGCTTCCAGGTCTTTTCCCTGGGGGAATTGAG
GAAGATCTCCGAGGAGGGGGTCAACTTCCGCTCCCACATCGACGGCTCCCGGCACTTCATCTCGCCCGAGGTCTCCATCG
CCATCCAGGAGGCGCTGGGCGCCGACATAGCCATGTGCTTCGACGAATGCCCCCCGTATCCGGCCGAGTATTCCTACGTG
CAGAAGTCTTTGGAACTCACCACCCGCTGGGCGCAGCGCTGCAAAAACGCGCACCAAAGGCCGGACCAGGCCCTTTTCGG
CATCGTCCAGGGGGGGATGCACCCCGAGCTTAGGCGGGAGAGCGCCAAGCAGATCACCGACATCGGTTTCGACGGCTATG
CCCTGGGGGGGCTCTCCGTCGGCGAGGAGAAGGAGGTCATGCACCGTATGATGCATGAATGCTCCGACATCCTCCCCGAG
AACTCCCCGCGCTACATCATGGGGATCGGCGCACCCGAGGACCTGGTGGAGGCGGTGAACGCCGGCTTCGACATGTTCGA
CTGCGTCATGCCGACCAGAAACGCGAGAAACGGCGCGCTCTTCACCAGTTTCGGCAGGATCAACATCAAGGCGGCCGTCT
TCGCGGAGGACCAGTCCCCGATAGACCCCGCCTGCAGCTGCTACGTCTGCAGGAACTACAGCAGGGCGTATCTGCGCCAT
CTGTACCGCAGCCAGGAGATACTGGCCTCCCGGCTGAACAGCTGGCACAACCTGCACTACTTCCTCGATCTGATGGCGCA
GATGCGCGCCGCCATCGGGCGCGGCGAGTTCTCAAGCTTCAGGAAGGATTTCTACGCGGCGAGGAGTCAGCAATTGTAG

Upstream 100 bases:

>100_bases
TAACGGAACTCTTCCCCACAGAGGGAAGCACTCCAACCCTTGCCCCCTCGCGCCGGGGGAGGGGGGAGTAAAACGAATAC
AAGCAGCAAACGGGATATTT

Downstream 100 bases:

>100_bases
TTCAACAAGAAACGAGAAAGGCCCTCCCCCTCCCTTGACGGGCCTGCGCGCCGAAGCGCTACGGCTCGCGAAGGCGGGAG
GGGGCCAGAGCTTTATCGCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MAAISFELIKKDPGSLARLGSLTTTHGSIQTPIFMPVGTQATVKAMTPEELVEVGSQIILANTYHLYLRPGHELIARLGG
LHRFMHWDRPMLTDSGGFQVFSLGELRKISEEGVNFRSHIDGSRHFISPEVSIAIQEALGADIAMCFDECPPYPAEYSYV
QKSLELTTRWAQRCKNAHQRPDQALFGIVQGGMHPELRRESAKQITDIGFDGYALGGLSVGEEKEVMHRMMHECSDILPE
NSPRYIMGIGAPEDLVEAVNAGFDMFDCVMPTRNARNGALFTSFGRINIKAAVFAEDQSPIDPACSCYVCRNYSRAYLRH
LYRSQEILASRLNSWHNLHYFLDLMAQMRAAIGRGEFSSFRKDFYAARSQQL

Sequences:

>Translated_372_residues
MAAISFELIKKDPGSLARLGSLTTTHGSIQTPIFMPVGTQATVKAMTPEELVEVGSQIILANTYHLYLRPGHELIARLGG
LHRFMHWDRPMLTDSGGFQVFSLGELRKISEEGVNFRSHIDGSRHFISPEVSIAIQEALGADIAMCFDECPPYPAEYSYV
QKSLELTTRWAQRCKNAHQRPDQALFGIVQGGMHPELRRESAKQITDIGFDGYALGGLSVGEEKEVMHRMMHECSDILPE
NSPRYIMGIGAPEDLVEAVNAGFDMFDCVMPTRNARNGALFTSFGRINIKAAVFAEDQSPIDPACSCYVCRNYSRAYLRH
LYRSQEILASRLNSWHNLHYFLDLMAQMRAAIGRGEFSSFRKDFYAARSQQL
>Mature_371_residues
AAISFELIKKDPGSLARLGSLTTTHGSIQTPIFMPVGTQATVKAMTPEELVEVGSQIILANTYHLYLRPGHELIARLGGL
HRFMHWDRPMLTDSGGFQVFSLGELRKISEEGVNFRSHIDGSRHFISPEVSIAIQEALGADIAMCFDECPPYPAEYSYVQ
KSLELTTRWAQRCKNAHQRPDQALFGIVQGGMHPELRRESAKQITDIGFDGYALGGLSVGEEKEVMHRMMHECSDILPEN
SPRYIMGIGAPEDLVEAVNAGFDMFDCVMPTRNARNGALFTSFGRINIKAAVFAEDQSPIDPACSCYVCRNYSRAYLRHL
YRSQEILASRLNSWHNLHYFLDLMAQMRAAIGRGEFSSFRKDFYAARSQQL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=363, Percent_Identity=45.4545454545455, Blast_Score=323, Evalue=1e-88,
Organism=Escherichia coli, GI1786607, Length=371, Percent_Identity=53.9083557951482, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17544680, Length=350, Percent_Identity=46.2857142857143, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI25146502, Length=183, Percent_Identity=27.3224043715847, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19920510, Length=364, Percent_Identity=46.4285714285714, Blast_Score=336, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21355311, Length=213, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41721; Mature: 41590

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAISFELIKKDPGSLARLGSLTTTHGSIQTPIFMPVGTQATVKAMTPEELVEVGSQIIL
CCCEEHHHHHCCCCHHHHHCCCEECCCCCCCCEEEECCCCCEEECCCHHHHHHCCCEEEE
ANTYHLYLRPGHELIARLGGLHRFMHWDRPMLTDSGGFQVFSLGELRKISEEGVNFRSHI
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECHHHHHHHHHCCCHHHHC
DGSRHFISPEVSIAIQEALGADIAMCFDECPPYPAEYSYVQKSLELTTRWAQRCKNAHQR
CCCCEECCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PDQALFGIVQGGMHPELRRESAKQITDIGFDGYALGGLSVGEEKEVMHRMMHECSDILPE
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCC
NSPRYIMGIGAPEDLVEAVNAGFDMFDCVMPTRNARNGALFTSFGRINIKAAVFAEDQSP
CCCCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCEEEEEEEEECCCCC
IDPACSCYVCRNYSRAYLRHLYRSQEILASRLNSWHNLHYFLDLMAQMRAAIGRGEFSSF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RKDFYAARSQQL
HHHHHHHHHCCC
>Mature Secondary Structure 
AAISFELIKKDPGSLARLGSLTTTHGSIQTPIFMPVGTQATVKAMTPEELVEVGSQIIL
CCEEHHHHHCCCCHHHHHCCCEECCCCCCCCEEEECCCCCEEECCCHHHHHHCCCEEEE
ANTYHLYLRPGHELIARLGGLHRFMHWDRPMLTDSGGFQVFSLGELRKISEEGVNFRSHI
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECHHHHHHHHHCCCHHHHC
DGSRHFISPEVSIAIQEALGADIAMCFDECPPYPAEYSYVQKSLELTTRWAQRCKNAHQR
CCCCEECCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PDQALFGIVQGGMHPELRRESAKQITDIGFDGYALGGLSVGEEKEVMHRMMHECSDILPE
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCC
NSPRYIMGIGAPEDLVEAVNAGFDMFDCVMPTRNARNGALFTSFGRINIKAAVFAEDQSP
CCCCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCEEEEEEEEECCCCC
IDPACSCYVCRNYSRAYLRHLYRSQEILASRLNSWHNLHYFLDLMAQMRAAIGRGEFSSF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RKDFYAARSQQL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]