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The map label for this gene is fieF [H]

Identifier: 197118807

GI number: 197118807

Start: 2816510

End: 2817409

Strand: Reverse

Name: fieF [H]

Synonym: Gbem_2428

Alternate gene names: 197118807

Gene position: 2817409-2816510 (Counterclockwise)

Preceding gene: 197118808

Following gene: 197118806

Centisome position: 61.05

GC content: 62.11

Gene sequence:

>900_bases
GTGCTGAGAGGGGAGCGCTTCAACAAGGCCGACCGCGCCATCAGGATCGGCTTCTGGCTCAACGCCGTGCTCATGGTCAT
GAAGCTGGCCGCCGGCCATTACGGCGACTCCGAGGCGGTCTTCGCCGACGGCGTCGAAAGCGCCTGTGACTTCATAGCGA
TCGGGATGACCCTGGTCGCGCTCAAGCTCGGGCGCAAGCCCTACGACGAAGACCACCCCTACGGCCACGGCAAGGCGGAA
AGCCTCTCGGCCATCTTCGTCTCCGTCATCATCGGCGCTACCGGCGCCTGGATCCTCTACGGCTCCATCTCCACCATGGT
GCACGGGACCTATCCCAAGCCCGCTTTGATCGCAGTCCTGGCTGCCGCGGTGACCATCGTTATCAAGGAACTCCTCTACC
GCTACTCGGTGCGGGTGGGTCGCTCCCTGGGGAGCCCGGCGCTCTTGGCCATCGCCAAGGACCACAGAAAAGACGCCGTC
ACCTCGGTCGCCACCCTGATCGGCGTCAGTTTCGCCTACTTCGGCGCCTCCGTCATGGATCCCATCGCCGCCGGAATCAC
CTCCTTCTTCATCTTCAGCATCGGCTACCAGACCTTCCGCTCCTCGGCCCACGAACTGATGGACGGCCAACCCGAGCAGG
AACTTTTGCGCGCCATAACGCTTTTGGCCGAGAGGGTGGAGGGGGTCGACCAGGTGCACGAAATAAGGGCCAGGCACTCG
GGGCAGTTCCTCATCGTCGATCTGAAGCTGGACATGGACCCCGAGATGACGGTGAAGCGCTCGCATGCCATAGCCACCCA
GGTGAAGGAGGAGATTTTCGACCAGTTCAACAACGTAGGCGACGTGATGATACACATAAACCCTTCCGACGAGCCGCACG
AGGATCTTAACCGCCTCTAG

Upstream 100 bases:

>100_bases
GGGCGCAGCGCGTCGACCTGGTCTGCTCCCCCGGGATCAACGTCTGGAACGGCAAAAAGAGCCTGCAATTGACCGTAAAG
GATCTGCGCGAGGTGCATGA

Downstream 100 bases:

>100_bases
AGCCTTTCTATCAGTCATCCCCACCGGTTTCTCAATCAATTAAGATTTACCCAAACAACTTTCTTGACACAGAGTCCGCT
TAGGTAGTATTACGATCAGT

Product: iron/zinc/nickel/cobalt/cadmium efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLRGERFNKADRAIRIGFWLNAVLMVMKLAAGHYGDSEAVFADGVESACDFIAIGMTLVALKLGRKPYDEDHPYGHGKAE
SLSAIFVSVIIGATGAWILYGSISTMVHGTYPKPALIAVLAAAVTIVIKELLYRYSVRVGRSLGSPALLAIAKDHRKDAV
TSVATLIGVSFAYFGASVMDPIAAGITSFFIFSIGYQTFRSSAHELMDGQPEQELLRAITLLAERVEGVDQVHEIRARHS
GQFLIVDLKLDMDPEMTVKRSHAIATQVKEEIFDQFNNVGDVMIHINPSDEPHEDLNRL

Sequences:

>Translated_299_residues
MLRGERFNKADRAIRIGFWLNAVLMVMKLAAGHYGDSEAVFADGVESACDFIAIGMTLVALKLGRKPYDEDHPYGHGKAE
SLSAIFVSVIIGATGAWILYGSISTMVHGTYPKPALIAVLAAAVTIVIKELLYRYSVRVGRSLGSPALLAIAKDHRKDAV
TSVATLIGVSFAYFGASVMDPIAAGITSFFIFSIGYQTFRSSAHELMDGQPEQELLRAITLLAERVEGVDQVHEIRARHS
GQFLIVDLKLDMDPEMTVKRSHAIATQVKEEIFDQFNNVGDVMIHINPSDEPHEDLNRL
>Mature_299_residues
MLRGERFNKADRAIRIGFWLNAVLMVMKLAAGHYGDSEAVFADGVESACDFIAIGMTLVALKLGRKPYDEDHPYGHGKAE
SLSAIFVSVIIGATGAWILYGSISTMVHGTYPKPALIAVLAAAVTIVIKELLYRYSVRVGRSLGSPALLAIAKDHRKDAV
TSVATLIGVSFAYFGASVMDPIAAGITSFFIFSIGYQTFRSSAHELMDGQPEQELLRAITLLAERVEGVDQVHEIRARHS
GQFLIVDLKLDMDPEMTVKRSHAIATQVKEEIFDQFNNVGDVMIHINPSDEPHEDLNRL

Specific function: Unknown

COG id: COG0053

COG function: function code P; Predicted Co/Zn/Cd cation transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family [H]

Homologues:

Organism=Escherichia coli, GI1790349, Length=272, Percent_Identity=25.7352941176471, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI72000060, Length=289, Percent_Identity=24.5674740484429, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI72000062, Length=289, Percent_Identity=24.5674740484429, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533508, Length=259, Percent_Identity=24.7104247104247, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115533506, Length=259, Percent_Identity=24.7104247104247, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI193210990, Length=311, Percent_Identity=24.1157556270096, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325032, Length=229, Percent_Identity=28.3842794759825, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323831, Length=227, Percent_Identity=26.431718061674, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 32746; Mature: 32746

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGERFNKADRAIRIGFWLNAVLMVMKLAAGHYGDSEAVFADGVESACDFIAIGMTLVA
CCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
LKLGRKPYDEDHPYGHGKAESLSAIFVSVIIGATGAWILYGSISTMVHGTYPKPALIAVL
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHEECCCCCHHHHHHH
AAAVTIVIKELLYRYSVRVGRSLGSPALLAIAKDHRKDAVTSVATLIGVSFAYFGASVMD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIAAGITSFFIFSIGYQTFRSSAHELMDGQPEQELLRAITLLAERVEGVDQVHEIRARHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GQFLIVDLKLDMDPEMTVKRSHAIATQVKEEIFDQFNNVGDVMIHINPSDEPHEDLNRL
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCC
>Mature Secondary Structure
MLRGERFNKADRAIRIGFWLNAVLMVMKLAAGHYGDSEAVFADGVESACDFIAIGMTLVA
CCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
LKLGRKPYDEDHPYGHGKAESLSAIFVSVIIGATGAWILYGSISTMVHGTYPKPALIAVL
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHEECCCCCHHHHHHH
AAAVTIVIKELLYRYSVRVGRSLGSPALLAIAKDHRKDAVTSVATLIGVSFAYFGASVMD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIAAGITSFFIFSIGYQTFRSSAHELMDGQPEQELLRAITLLAERVEGVDQVHEIRARHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GQFLIVDLKLDMDPEMTVKRSHAIATQVKEEIFDQFNNVGDVMIHINPSDEPHEDLNRL
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]