Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 197118690

GI number: 197118690

Start: 2683020

End: 2683682

Strand: Reverse

Name: nth [H]

Synonym: Gbem_2309

Alternate gene names: 197118690

Gene position: 2683682-2683020 (Counterclockwise)

Preceding gene: 197118691

Following gene: 197118689

Centisome position: 58.15

GC content: 56.71

Gene sequence:

>663_bases
ATGAAGGACGATCAGATACACCAGGCCATCGCCATCCTCAAGGATGCGGTGCTGACAAAAGGGTGGGTGACTCCGGCGGT
CACTATCGTGGCTACGCAGGACCGCGACCCCTACAAGGTGCTGGTCTCCTGCATTCTATCCCTGCGAACCCGCGATCAGA
CCACAGCCGAGGCTTCCCAGCGCTTGTTTGCGCTTGCCGATACACCGCAGAAGATGACGGAACTCAGTGTCCCCGAGATC
GAGCAGGCCATCTACCCCGTCGGGTTTTACCGGGTCAAGGCACAGCAGATTTTAGAACTCTCGTTCCAAATCGGTGAGCT
GCACCAGGGGAGGGTGCCCGACGAATTGGAGACCCTGCTGACTTTCAAGGGAGTGGGGCGCAAGACGGCGAACCTGGTGC
TGACGCTTGGATACGGCAAGCCCGGCATCTGCGTCGACATTCACGTGCACAGGATCTGCAACAGGTGGGGGTATGTAAAA
ACAGTCAATCCTGAGCAGACAGAGGGTGCTTTGAGAAAGAAGCTCCCGCCCGAGTACTGGATCATAATCAACGATCTTCT
AGTGACTTTCGGGCAAAACCAGTGCACACCTGTCTCACCGCGCTGCTCCACCTGTCCCCTCTATGCGCTCTGTGACAGGG
TCGCTGTTACTAAGTCTAGATAA

Upstream 100 bases:

>100_bases
GGTGAGGGAAGAAGCATCGGCATTCTCTTTGTCTGCGTGGGACTTCTTCTGTCAATCCTGGGAGTGCTGGTGATGCGTCT
CGTGCGCAACAGGATCTAGC

Downstream 100 bases:

>100_bases
AACAGTGTTGCACTAAAGCGCTCTTTGAGTATAATGAGTCCTTGAGTTTTTTAAGCCACATTCTCACCGCCAGTTAGACG
CGGTGGAAAATGTTATCCGC

Product: endonuclease III-like DNA glycosidase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKDDQIHQAIAILKDAVLTKGWVTPAVTIVATQDRDPYKVLVSCILSLRTRDQTTAEASQRLFALADTPQKMTELSVPEI
EQAIYPVGFYRVKAQQILELSFQIGELHQGRVPDELETLLTFKGVGRKTANLVLTLGYGKPGICVDIHVHRICNRWGYVK
TVNPEQTEGALRKKLPPEYWIIINDLLVTFGQNQCTPVSPRCSTCPLYALCDRVAVTKSR

Sequences:

>Translated_220_residues
MKDDQIHQAIAILKDAVLTKGWVTPAVTIVATQDRDPYKVLVSCILSLRTRDQTTAEASQRLFALADTPQKMTELSVPEI
EQAIYPVGFYRVKAQQILELSFQIGELHQGRVPDELETLLTFKGVGRKTANLVLTLGYGKPGICVDIHVHRICNRWGYVK
TVNPEQTEGALRKKLPPEYWIIINDLLVTFGQNQCTPVSPRCSTCPLYALCDRVAVTKSR
>Mature_220_residues
MKDDQIHQAIAILKDAVLTKGWVTPAVTIVATQDRDPYKVLVSCILSLRTRDQTTAEASQRLFALADTPQKMTELSVPEI
EQAIYPVGFYRVKAQQILELSFQIGELHQGRVPDELETLLTFKGVGRKTANLVLTLGYGKPGICVDIHVHRICNRWGYVK
TVNPEQTEGALRKKLPPEYWIIINDLLVTFGQNQCTPVSPRCSTCPLYALCDRVAVTKSR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=43.5028248587571, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1787920, Length=177, Percent_Identity=35.5932203389831, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1789331, Length=185, Percent_Identity=28.1081081081081, Blast_Score=73, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=197, Percent_Identity=41.1167512690355, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=32.0855614973262, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319304, Length=167, Percent_Identity=34.1317365269461, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=40.6779661016949, Blast_Score=133, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24649; Mature: 24649

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDDQIHQAIAILKDAVLTKGWVTPAVTIVATQDRDPYKVLVSCILSLRTRDQTTAEASQ
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHH
RLFALADTPQKMTELSVPEIEQAIYPVGFYRVKAQQILELSFQIGELHQGRVPDELETLL
HEEEECCCHHHHHHCCCHHHHHHHCCCHHEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TFKGVGRKTANLVLTLGYGKPGICVDIHVHRICNRWGYVKTVNPEQTEGALRKKLPPEYW
HHHCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCEE
IIINDLLVTFGQNQCTPVSPRCSTCPLYALCDRVAVTKSR
EEHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKDDQIHQAIAILKDAVLTKGWVTPAVTIVATQDRDPYKVLVSCILSLRTRDQTTAEASQ
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHH
RLFALADTPQKMTELSVPEIEQAIYPVGFYRVKAQQILELSFQIGELHQGRVPDELETLL
HEEEECCCHHHHHHCCCHHHHHHHCCCHHEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TFKGVGRKTANLVLTLGYGKPGICVDIHVHRICNRWGYVKTVNPEQTEGALRKKLPPEYW
HHHCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCEE
IIINDLLVTFGQNQCTPVSPRCSTCPLYALCDRVAVTKSR
EEHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]