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The map label for this gene is cpsA1 [H]

Identifier: 197118609

GI number: 197118609

Start: 2594004

End: 2595296

Strand: Reverse

Name: cpsA1 [H]

Synonym: Gbem_2228

Alternate gene names: 197118609

Gene position: 2595296-2594004 (Counterclockwise)

Preceding gene: 197118615

Following gene: 197118606

Centisome position: 56.23

GC content: 65.04

Gene sequence:

>1293_bases
ATGAACGACCAAGTAACCAGCGGCACTTTCACCGGCATGGTTGAAGCCGAACTCCCCTCCCTGGCCGCCATCTACCGACA
GCTGCACGCTCAGCCCGAACTCTCGGGGCAGGAGGAGCAGACCTCGGCACTTGTCGCCGCCGAACTGCGCGCCTTGGGTT
ACACGGTGACGGAAGGGGTGGGAAGATACCGCAATTACGACTGGCCTGGCTACGGCGTCGTCGCCGTTCTTTCCAACGGC
ATCGGGCCCACCGTGCTGCTTCGGGCCGACATGGACGCGCTCCCGGTTCAGGAAAAGACCGGCCTTCCCTATGCCAGCCA
GGTCAAGGGGACCTACCGCGACGGCAACGAGGTGCCGGTAATGCATGCCTGCGGGCACGATATCCATGTCACGGTCTTAT
TGGGCGTGGCGCGCCTTATGGCGCGGCTTAGGAGGAACTGGCAGGGATCCCTGGTTCTGGTCGCCCAACCCGGCGAAGAG
GGGGGCGGCGGGGCAGACGCCATGTTGGATGACGGCGCCTACGGCTATTGCCCTAAGCCCGACTTTGCCCTGGCGCTGCA
CAGCACCCTGCACCTTAAGGCCGGCAGCGCCGGGTACGCCCCGGGGAACTTCATGGCGAGCTTGTGCGAGCTGGAGGTCG
TAGTCCGCGGCGTCGGCTCCCATGGCTCGGCCCCCGAGTGCGGCAAGGACCCGGTCGTCATGGCCGCCCAACTGGTGCTC
GCCCTGCAGACCGTCGTCAGCCGGGAGAAAAGCCCCAGAGAACCGGCAGTCTTAAGCGTCGGCTCCATTCACGGCGGCGC
AGCCAGCAATGTGATACCGGACGAGGTCGTACTGCAACTCAGCGTCAGGACCTATGACGACGGCGTTCGCGACCGTATCG
TCGAATCGGTGCGCCGGATGGCCGCCGGCGTGGCGCTGGTCGCCGGGGTGCCCGATGACCGGTCTCCGCTCGTGAAGGTG
AAGGTCTCCCATCCGGCGATCTACAACGACCCGGAGTTGGCCGAACGGGTGGCGGCTTCGCTGCGCCTGGCCTTAGGCTC
CGGCAACGTCTACCGCAGCGAACCGAAGATGGTGAGTGAGGATTTCGGCTCCTGGAGCCTGGAAGGGAAGATTCCCATCT
GCATGTTCTGGCTGGGCGCAGCCGCCCCGGAAAGCTTCGAGGCGAGCCGGAAAAGCGGAATGCCGCTTCCTTCGCACCAT
TCTCCTCTGTTCGCCCCATTACCGGAGCCGACCATTCGGGCAGGTGTTGCCGGCCTTGCCACGGCGGCGCTGGATCTCTT
CAAAACGACCTAA

Upstream 100 bases:

>100_bases
TATCCATAGTACCCTTGCGGCGTCCAGCGGCGCGACAGACGCTGTTATCCCCCTTACTGCCACGCAACCACAACCTCCGA
TACAGCAGGTGCGATTTCGT

Downstream 100 bases:

>100_bases
GAAAAAGAGGGGATAAGTCGATGACTTGTCCCCTCTTCGTTCCGATTCCTCTTTACCTTTTATCGAGTGTCTCTTTCCTC
TCGCTGTCCCCCCCTCGGTT

Product: metal-dependent amidase/aminoacylase/carboxypeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNDQVTSGTFTGMVEAELPSLAAIYRQLHAQPELSGQEEQTSALVAAELRALGYTVTEGVGRYRNYDWPGYGVVAVLSNG
IGPTVLLRADMDALPVQEKTGLPYASQVKGTYRDGNEVPVMHACGHDIHVTVLLGVARLMARLRRNWQGSLVLVAQPGEE
GGGGADAMLDDGAYGYCPKPDFALALHSTLHLKAGSAGYAPGNFMASLCELEVVVRGVGSHGSAPECGKDPVVMAAQLVL
ALQTVVSREKSPREPAVLSVGSIHGGAASNVIPDEVVLQLSVRTYDDGVRDRIVESVRRMAAGVALVAGVPDDRSPLVKV
KVSHPAIYNDPELAERVAASLRLALGSGNVYRSEPKMVSEDFGSWSLEGKIPICMFWLGAAAPESFEASRKSGMPLPSHH
SPLFAPLPEPTIRAGVAGLATAALDLFKTT

Sequences:

>Translated_430_residues
MNDQVTSGTFTGMVEAELPSLAAIYRQLHAQPELSGQEEQTSALVAAELRALGYTVTEGVGRYRNYDWPGYGVVAVLSNG
IGPTVLLRADMDALPVQEKTGLPYASQVKGTYRDGNEVPVMHACGHDIHVTVLLGVARLMARLRRNWQGSLVLVAQPGEE
GGGGADAMLDDGAYGYCPKPDFALALHSTLHLKAGSAGYAPGNFMASLCELEVVVRGVGSHGSAPECGKDPVVMAAQLVL
ALQTVVSREKSPREPAVLSVGSIHGGAASNVIPDEVVLQLSVRTYDDGVRDRIVESVRRMAAGVALVAGVPDDRSPLVKV
KVSHPAIYNDPELAERVAASLRLALGSGNVYRSEPKMVSEDFGSWSLEGKIPICMFWLGAAAPESFEASRKSGMPLPSHH
SPLFAPLPEPTIRAGVAGLATAALDLFKTT
>Mature_430_residues
MNDQVTSGTFTGMVEAELPSLAAIYRQLHAQPELSGQEEQTSALVAAELRALGYTVTEGVGRYRNYDWPGYGVVAVLSNG
IGPTVLLRADMDALPVQEKTGLPYASQVKGTYRDGNEVPVMHACGHDIHVTVLLGVARLMARLRRNWQGSLVLVAQPGEE
GGGGADAMLDDGAYGYCPKPDFALALHSTLHLKAGSAGYAPGNFMASLCELEVVVRGVGSHGSAPECGKDPVVMAAQLVL
ALQTVVSREKSPREPAVLSVGSIHGGAASNVIPDEVVLQLSVRTYDDGVRDRIVESVRRMAAGVALVAGVPDDRSPLVKV
KVSHPAIYNDPELAERVAASLRLALGSGNVYRSEPKMVSEDFGSWSLEGKIPICMFWLGAAAPESFEASRKSGMPLPSHH
SPLFAPLPEPTIRAGVAGLATAALDLFKTT

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=334, Percent_Identity=28.7425149700599, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 45411; Mature: 45411

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQVTSGTFTGMVEAELPSLAAIYRQLHAQPELSGQEEQTSALVAAELRALGYTVTEGV
CCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHH
GRYRNYDWPGYGVVAVLSNGIGPTVLLRADMDALPVQEKTGLPYASQVKGTYRDGNEVPV
HHHCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHCCCCCHHHCCCCCCCCCCCCE
MHACGHDIHVTVLLGVARLMARLRRNWQGSLVLVAQPGEEGGGGADAMLDDGAYGYCPKP
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEECCCCCCCCCCC
DFALALHSTLHLKAGSAGYAPGNFMASLCELEVVVRGVGSHGSAPECGKDPVVMAAQLVL
CCEEEEECEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
ALQTVVSREKSPREPAVLSVGSIHGGAASNVIPDEVVLQLSVRTYDDGVRDRIVESVRRM
HHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
AAGVALVAGVPDDRSPLVKVKVSHPAIYNDPELAERVAASLRLALGSGNVYRSEPKMVSE
HHCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCEECCCCCHHHH
DFGSWSLEGKIPICMFWLGAAAPESFEASRKSGMPLPSHHSPLFAPLPEPTIRAGVAGLA
CCCCEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
TAALDLFKTT
HHHHHHHHCC
>Mature Secondary Structure
MNDQVTSGTFTGMVEAELPSLAAIYRQLHAQPELSGQEEQTSALVAAELRALGYTVTEGV
CCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHH
GRYRNYDWPGYGVVAVLSNGIGPTVLLRADMDALPVQEKTGLPYASQVKGTYRDGNEVPV
HHHCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHCCCCCHHHCCCCCCCCCCCCE
MHACGHDIHVTVLLGVARLMARLRRNWQGSLVLVAQPGEEGGGGADAMLDDGAYGYCPKP
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEECCCCCCCCCCC
DFALALHSTLHLKAGSAGYAPGNFMASLCELEVVVRGVGSHGSAPECGKDPVVMAAQLVL
CCEEEEECEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
ALQTVVSREKSPREPAVLSVGSIHGGAASNVIPDEVVLQLSVRTYDDGVRDRIVESVRRM
HHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
AAGVALVAGVPDDRSPLVKVKVSHPAIYNDPELAERVAASLRLALGSGNVYRSEPKMVSE
HHCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCEECCCCCHHHH
DFGSWSLEGKIPICMFWLGAAAPESFEASRKSGMPLPSHHSPLFAPLPEPTIRAGVAGLA
CCCCEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
TAALDLFKTT
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]