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The map label for this gene is cspLA [H]

Identifier: 197118603

GI number: 197118603

Start: 2586456

End: 2586656

Strand: Direct

Name: cspLA [H]

Synonym: Gbem_2222

Alternate gene names: 197118603

Gene position: 2586456-2586656 (Clockwise)

Preceding gene: 197118600

Following gene: 197118607

Centisome position: 56.04

GC content: 49.75

Gene sequence:

>201_bases
ATGGTAAACGGAACTGTGAAATGGTTTAACGACAGCAAGGGTTTTGGCTTTCTCGAGCAGGAAAATGGCGAGGACGTATT
CGTTCATTTCTCCGCTATCAACGGCGACGGCTTCAAATCCCTCACCGAAGGCGACAGCGTGACTTTCGAAATCGTAAAGG
GACCGAAAGGGCTTCAGGCAGCTAACGTTTCCAGGGCTTAA

Upstream 100 bases:

>100_bases
TCAAATTATAAGATTGACATGGTGCAGGAGATGCTATATGTTTACGCATCCGTGGATTTCCGGAACACTAACACAGCAGT
AATATGGGAGAAGTAAGTAA

Downstream 100 bases:

>100_bases
TCACATAGCGTAGAACTTTATCGAAGGCCCCGGAGGAATCCGGGGCCTTTTTTCGTTTCTTGGCCGTAGACCTACCCCTC
CCCAACCCTCCCCTTCCAGG

Product: cold shock DNA/RNA-binding protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAINGDGFKSLTEGDSVTFEIVKGPKGLQAANVSRA

Sequences:

>Translated_66_residues
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAINGDGFKSLTEGDSVTFEIVKGPKGLQAANVSRA
>Mature_66_residues
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAINGDGFKSLTEGDSVTFEIVKGPKGLQAANVSRA

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=63.4920634920635, Blast_Score=79, Evalue=4e-17,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=60.9375, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=57.8125, Blast_Score=72, Evalue=5e-15,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=51.5625, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=57.1428571428571, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1787834, Length=63, Percent_Identity=55.5555555555556, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI2367114, Length=63, Percent_Identity=50.7936507936508, Blast_Score=64, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7126; Mature: 7126

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAINGDGFKSLTEGDSVTFEIVKGPKGLQA
CCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCC
ANVSRA
CCCCCC
>Mature Secondary Structure
MVNGTVKWFNDSKGFGFLEQENGEDVFVHFSAINGDGFKSLTEGDSVTFEIVKGPKGLQA
CCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCC
ANVSRA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]