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The map label for this gene is paaK-2 [H]

Identifier: 197118573

GI number: 197118573

Start: 2551500

End: 2552804

Strand: Reverse

Name: paaK-2 [H]

Synonym: Gbem_2192

Alternate gene names: 197118573

Gene position: 2552804-2551500 (Counterclockwise)

Preceding gene: 197118574

Following gene: 197118572

Centisome position: 55.31

GC content: 61.38

Gene sequence:

>1305_bases
ATGTTCTTCAACGAGGAGTTCGAGACGCTTCCCAGGGAGGCTATCGAGGCACTGCAGCTCAAAAGGCTCAAGTCGATGGT
GGCGCGCGTTCAACAAAACGTCCCCTTCTACAAGGAGTCGCTGGCCAAGGCGGGAGTCAACGCCGATTCCATAAAGTCCC
TGGCGGACCTGGCCCGGCTCCCCTTCACCTACAAGCAGGACATGCGCGACTCCTACCCGTACCGCCTCTTCGCGGTGCCG
ATGGAGGACATCGTCCGCATCCACGCCTCTTCCGGCACCACCGGCAAACCTACGGTGGTCGGCTACACCCAGAAGGACAT
CGACACCTGGAGCGAGCTGATGGCCCGTTCGTTTGTAGCCGCCGGGGTGCACAAGGGCGACATCATTCACAACTCTTACG
GTTACGGCCTCTTCACCGGCGGCCTGGGCGCGCATTACGGCGCCGAGCGGCTCGGCGCGTCGGTCATCCCGATGTCCGGG
GGTAACACCAAGAAGCAGATCATGATCATGCAGGACTTCGGTTCCACCGTCCTCACCTGCACCCCGTCCTACTCGCTCTA
CATGGCGGAGGCCGCCAAGGAAGAAGGGATCGACTTCCGCGACCTGAAGCTCAAGGTCGGCATCTTCGGCGCGGAGCCCT
GGTCCGAGGCGATGCGCCTCGACATCGAGGAGAAGCTGAACCTCTCCGCGGTCGACATCTACGGACTCTCCGAGATCATG
GGACCCGGCGTCGCCATCGAGTGCTGCGAGGCGAAAAAGGGGCTCCACGTCTGGGAGGACCACTTCATCCCCGAGATCAT
CAACCCCGAGACCGGCGAAGTGCTCCCAGAAGGGCAGAAGGGGGAGCTGGTCATCACCACCATCACCAAGGAAGGGATTC
CGCTCATCCGCTACCGGACCCGCGACATCACCTCCATCACCTACGAGCCCTGCATCTGCGGCAGGACCCATGCCCGCATA
GCCCGCATGAGCGGCAGAAGCGACGACATGCTGATCATCCGCGGCGTCAACGTCTTCCCGTCGCAGATCGAGGCGATCCT
CATGGGGGTCGAAGGTGTCGAGCCGCACTACGTCCTCATCGTGGACAGGAAGGACAACCTGGACACGCTCGAAGTGCAGG
TCGAGGTGGGCGAGAACATTTTCTCCGACGAGATCAAGCACCTCCAGGCGATCTCCACCAAGATCGAGAAGCAGATAAAG
GAGATGCTGGGGGTCACCTGCCGCGTAAGGCTCGTGGAACCCAAAAGCATCACCCGCAGCGAAGGCAAGGCCAAGAGGGT
CATCGACAACAGGAACAAAGCCTAA

Upstream 100 bases:

>100_bases
TCCCTGTATAAAGCCTTTTGTTTTAACCGCTTTTCCTCAGGAACCTTGTGGTGAAGCTTTTTGGTTTGATCTAACGTTTC
GGTTTTTTCAGGAGTAAGTC

Downstream 100 bases:

>100_bases
GGGGGATACCATGTACGTAGAACAGATCTCCATATTCATCGAAAACAAGTTCGGACGTCTGGCAGAGGTGACCCGCATCC
TGGGCGATGCAGGCGTCAAC

Product: phenylacetate--coenzyme A ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MFFNEEFETLPREAIEALQLKRLKSMVARVQQNVPFYKESLAKAGVNADSIKSLADLARLPFTYKQDMRDSYPYRLFAVP
MEDIVRIHASSGTTGKPTVVGYTQKDIDTWSELMARSFVAAGVHKGDIIHNSYGYGLFTGGLGAHYGAERLGASVIPMSG
GNTKKQIMIMQDFGSTVLTCTPSYSLYMAEAAKEEGIDFRDLKLKVGIFGAEPWSEAMRLDIEEKLNLSAVDIYGLSEIM
GPGVAIECCEAKKGLHVWEDHFIPEIINPETGEVLPEGQKGELVITTITKEGIPLIRYRTRDITSITYEPCICGRTHARI
ARMSGRSDDMLIIRGVNVFPSQIEAILMGVEGVEPHYVLIVDRKDNLDTLEVQVEVGENIFSDEIKHLQAISTKIEKQIK
EMLGVTCRVRLVEPKSITRSEGKAKRVIDNRNKA

Sequences:

>Translated_434_residues
MFFNEEFETLPREAIEALQLKRLKSMVARVQQNVPFYKESLAKAGVNADSIKSLADLARLPFTYKQDMRDSYPYRLFAVP
MEDIVRIHASSGTTGKPTVVGYTQKDIDTWSELMARSFVAAGVHKGDIIHNSYGYGLFTGGLGAHYGAERLGASVIPMSG
GNTKKQIMIMQDFGSTVLTCTPSYSLYMAEAAKEEGIDFRDLKLKVGIFGAEPWSEAMRLDIEEKLNLSAVDIYGLSEIM
GPGVAIECCEAKKGLHVWEDHFIPEIINPETGEVLPEGQKGELVITTITKEGIPLIRYRTRDITSITYEPCICGRTHARI
ARMSGRSDDMLIIRGVNVFPSQIEAILMGVEGVEPHYVLIVDRKDNLDTLEVQVEVGENIFSDEIKHLQAISTKIEKQIK
EMLGVTCRVRLVEPKSITRSEGKAKRVIDNRNKA
>Mature_434_residues
MFFNEEFETLPREAIEALQLKRLKSMVARVQQNVPFYKESLAKAGVNADSIKSLADLARLPFTYKQDMRDSYPYRLFAVP
MEDIVRIHASSGTTGKPTVVGYTQKDIDTWSELMARSFVAAGVHKGDIIHNSYGYGLFTGGLGAHYGAERLGASVIPMSG
GNTKKQIMIMQDFGSTVLTCTPSYSLYMAEAAKEEGIDFRDLKLKVGIFGAEPWSEAMRLDIEEKLNLSAVDIYGLSEIM
GPGVAIECCEAKKGLHVWEDHFIPEIINPETGEVLPEGQKGELVITTITKEGIPLIRYRTRDITSITYEPCICGRTHARI
ARMSGRSDDMLIIRGVNVFPSQIEAILMGVEGVEPHYVLIVDRKDNLDTLEVQVEVGENIFSDEIKHLQAISTKIEKQIK
EMLGVTCRVRLVEPKSITRSEGKAKRVIDNRNKA

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=429, Percent_Identity=53.1468531468531, Blast_Score=465, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48370; Mature: 48370

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFNEEFETLPREAIEALQLKRLKSMVARVQQNVPFYKESLAKAGVNADSIKSLADLARL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHC
PFTYKQDMRDSYPYRLFAVPMEDIVRIHASSGTTGKPTVVGYTQKDIDTWSELMARSFVA
CCHHHHHHCCCCCEEEEEECHHHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
AGVHKGDIIHNSYGYGLFTGGLGAHYGAERLGASVIPMSGGNTKKQIMIMQDFGSTVLTC
HCCCCCCEEECCCCCEEEECCCCCCCCHHHHCCEEEECCCCCCCEEEEEEECCCCEEEEE
TPSYSLYMAEAAKEEGIDFRDLKLKVGIFGAEPWSEAMRLDIEEKLNLSAVDIYGLSEIM
CCCCEEEEEHHHHHHCCCEEEEEEEEEEECCCCHHHHHHCCHHHHCCCEEEEECCHHHHC
GPGVAIECCEAKKGLHVWEDHFIPEIINPETGEVLPEGQKGELVITTITKEGIPLIRYRT
CCCCEEEEECCCCCCEEHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEC
RDITSITYEPCICGRTHARIARMSGRSDDMLIIRGVNVFPSQIEAILMGVEGVEPHYVLI
CCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEE
VDRKDNLDTLEVQVEVGENIFSDEIKHLQAISTKIEKQIKEMLGVTCRVRLVEPKSITRS
EECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
EGKAKRVIDNRNKA
CCCHHHHHCCCCCC
>Mature Secondary Structure
MFFNEEFETLPREAIEALQLKRLKSMVARVQQNVPFYKESLAKAGVNADSIKSLADLARL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHC
PFTYKQDMRDSYPYRLFAVPMEDIVRIHASSGTTGKPTVVGYTQKDIDTWSELMARSFVA
CCHHHHHHCCCCCEEEEEECHHHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
AGVHKGDIIHNSYGYGLFTGGLGAHYGAERLGASVIPMSGGNTKKQIMIMQDFGSTVLTC
HCCCCCCEEECCCCCEEEECCCCCCCCHHHHCCEEEECCCCCCCEEEEEEECCCCEEEEE
TPSYSLYMAEAAKEEGIDFRDLKLKVGIFGAEPWSEAMRLDIEEKLNLSAVDIYGLSEIM
CCCCEEEEEHHHHHHCCCEEEEEEEEEEECCCCHHHHHHCCHHHHCCCEEEEECCHHHHC
GPGVAIECCEAKKGLHVWEDHFIPEIINPETGEVLPEGQKGELVITTITKEGIPLIRYRT
CCCCEEEEECCCCCCEEHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEC
RDITSITYEPCICGRTHARIARMSGRSDDMLIIRGVNVFPSQIEAILMGVEGVEPHYVLI
CCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEE
VDRKDNLDTLEVQVEVGENIFSDEIKHLQAISTKIEKQIKEMLGVTCRVRLVEPKSITRS
EECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
EGKAKRVIDNRNKA
CCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]