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The map label for this gene is fadA [H]

Identifier: 197118509

GI number: 197118509

Start: 2473251

End: 2474423

Strand: Direct

Name: fadA [H]

Synonym: Gbem_2127

Alternate gene names: 197118509

Gene position: 2473251-2474423 (Clockwise)

Preceding gene: 197118508

Following gene: 197118510

Centisome position: 53.59

GC content: 65.9

Gene sequence:

>1173_bases
ATGAGAACCGCATACATAGTTATCGCCCTGCGCACTCCGGCGACCAAGGCCTACAAAGGGAAACTGAGGCAGGTGCGCCC
CGACGACCTGGCCGCCGCGGCCATCAAAGGGGTCATCGACGCCACCGGCATCGACCCGGCAGAACTCGACGACATTATCA
TCGGCTGCGCCTTCCCCGAGGCCGAACAGGGGATGAACGTGGCGCGCATCGCCGCTTTCAAGGCGGGCGTCCCGTACCAG
GTTCCCTGCCAGACGGTGAACCGCTTCTGCTCCTCCGGGCTGCAGACCATCGCCACCGCCGCCGAGCGCATCATGGCCGG
CTTCGCCGACTGCATCGTCGCGGGGGGGACCGAGAGCATGTCGCTCATTCCCATGGGGGGGAACAAATTCAGCGCCAACC
CGGGGCTCGTCCATGCCTGGCCCGAGACCTACGCCTCGATGGGAATCACCGCGGAGCTCATGGTCGAGAAGTACGGGGTG
TCCCGCGAGGACCAGGACGCCTTCGCCGCGGCGAGCCACCAAAAGGCGGCACAGGCGATAAGGGAGGGGAGGTTTGCCGA
CGAGATCGTCCCGGTCAAAGCTCAAATCTGCGGCCTGGACGGCCTGAAGATGAAGTGCAGCGTGGAACAGGTGAGCGTCG
ACGACGGCGTCCGCGCCGACACGACGGTTGCGGCACTCGCGAAGCTCAAGCCCGCCTTCAAGGCCAACGGCAGCGTTACC
GCAGGCAACTCCTCCCAGATGACCGACGGGGCCGCGGCGGCGCTGGTCGTCTCCGAGGATTTCCTGAAGCGAACCGGCTT
GCAGCCGATGGCGCGCTTTGTCTGCTTCGCCGTCAAGGGGGTCCCGCCCGAGATCATGGGGACCGGCCCCATCGAGGCGA
TTCCAGCGGCGTTAAAGCTTGCCGGGCTCTCTCTGGATCAGGTCTCCCTCTTCGAGCTGAACGAAGCCTTCGCCGTCCAG
TCCCTGGTCTGCATCGACGAGCTGAAGCTCGACCCGGAAAGGGTGAACGTGAACGGCGGCGCCATCGCCATGGGGCATCC
GCTGGGGTGCACCGGGACCAAGCTGACCGCGACGCTGTTGCACGAATTGCGGCGCCGGAAGGAGCGCTACGGCTGCGTCT
CCATGTGCATCGGCGGCGGCATGGGCGCGGCAGGGATCTTCGAGATGGTCTAG

Upstream 100 bases:

>100_bases
AGTTCTGCGGCCAGAAGAAGACCCTGGAGCGCATCCAGCACATGCTGAAAAAAGGGAGGCCTCTCAGGAACTAGGGGGGC
GCTACCATAGGAGAAACACC

Downstream 100 bases:

>100_bases
TGCGATGGTGCTACCTGCCAGTTGAGTGGGAGTGAGGGTTGTGCTCCCCTCGCCCTCTGGGGGGGGCGGGGGTGAGGGAA
GGGGTGTGCGGGGGTGAGGG

Product: thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MRTAYIVIALRTPATKAYKGKLRQVRPDDLAAAAIKGVIDATGIDPAELDDIIIGCAFPEAEQGMNVARIAAFKAGVPYQ
VPCQTVNRFCSSGLQTIATAAERIMAGFADCIVAGGTESMSLIPMGGNKFSANPGLVHAWPETYASMGITAELMVEKYGV
SREDQDAFAAASHQKAAQAIREGRFADEIVPVKAQICGLDGLKMKCSVEQVSVDDGVRADTTVAALAKLKPAFKANGSVT
AGNSSQMTDGAAAALVVSEDFLKRTGLQPMARFVCFAVKGVPPEIMGTGPIEAIPAALKLAGLSLDQVSLFELNEAFAVQ
SLVCIDELKLDPERVNVNGGAIAMGHPLGCTGTKLTATLLHELRRRKERYGCVSMCIGGGMGAAGIFEMV

Sequences:

>Translated_390_residues
MRTAYIVIALRTPATKAYKGKLRQVRPDDLAAAAIKGVIDATGIDPAELDDIIIGCAFPEAEQGMNVARIAAFKAGVPYQ
VPCQTVNRFCSSGLQTIATAAERIMAGFADCIVAGGTESMSLIPMGGNKFSANPGLVHAWPETYASMGITAELMVEKYGV
SREDQDAFAAASHQKAAQAIREGRFADEIVPVKAQICGLDGLKMKCSVEQVSVDDGVRADTTVAALAKLKPAFKANGSVT
AGNSSQMTDGAAAALVVSEDFLKRTGLQPMARFVCFAVKGVPPEIMGTGPIEAIPAALKLAGLSLDQVSLFELNEAFAVQ
SLVCIDELKLDPERVNVNGGAIAMGHPLGCTGTKLTATLLHELRRRKERYGCVSMCIGGGMGAAGIFEMV
>Mature_390_residues
MRTAYIVIALRTPATKAYKGKLRQVRPDDLAAAAIKGVIDATGIDPAELDDIIIGCAFPEAEQGMNVARIAAFKAGVPYQ
VPCQTVNRFCSSGLQTIATAAERIMAGFADCIVAGGTESMSLIPMGGNKFSANPGLVHAWPETYASMGITAELMVEKYGV
SREDQDAFAAASHQKAAQAIREGRFADEIVPVKAQICGLDGLKMKCSVEQVSVDDGVRADTTVAALAKLKPAFKANGSVT
AGNSSQMTDGAAAALVVSEDFLKRTGLQPMARFVCFAVKGVPPEIMGTGPIEAIPAALKLAGLSLDQVSLFELNEAFAVQ
SLVCIDELKLDPERVNVNGGAIAMGHPLGCTGTKLTATLLHELRRRKERYGCVSMCIGGGMGAAGIFEMV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=391, Percent_Identity=46.8030690537084, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=39.75, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI148539872, Length=394, Percent_Identity=39.3401015228426, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=33.9152119700748, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI194353979, Length=384, Percent_Identity=34.8958333333333, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI4504327, Length=439, Percent_Identity=33.4851936218679, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI48994986, Length=395, Percent_Identity=44.5569620253165, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1787663, Length=412, Percent_Identity=42.7184466019417, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=39.5522388059701, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=42.1836228287841, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788683, Length=428, Percent_Identity=32.0093457943925, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=38.4039900249377, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=35.264483627204, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI25147385, Length=399, Percent_Identity=33.0827067669173, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=407, Percent_Identity=33.1695331695332, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17535917, Length=400, Percent_Identity=29.25, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=38.8471177944862, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6325229, Length=411, Percent_Identity=36.4963503649635, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24655093, Length=397, Percent_Identity=42.0654911838791, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=40.6015037593985, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=36.5482233502538, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137578, Length=412, Percent_Identity=31.7961165048544, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24585051, Length=383, Percent_Identity=24.0208877284595, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40888; Mature: 40888

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTAYIVIALRTPATKAYKGKLRQVRPDDLAAAAIKGVIDATGIDPAELDDIIIGCAFPE
CCEEEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHCCEEEECCCCC
AEQGMNVARIAAFKAGVPYQVPCQTVNRFCSSGLQTIATAAERIMAGFADCIVAGGTESM
HHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCE
SLIPMGGNKFSANPGLVHAWPETYASMGITAELMVEKYGVSREDQDAFAAASHQKAAQAI
EEEEECCCEECCCCCEEEECHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
REGRFADEIVPVKAQICGLDGLKMKCSVEQVSVDDGVRADTTVAALAKLKPAFKANGSVT
HCCCCHHHEECCHHEEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCHHHCCCCCEE
AGNSSQMTDGAAAALVVSEDFLKRTGLQPMARFVCFAVKGVPPEIMGTGPIEAIPAALKL
CCCCCCCCCCHHEEEEECHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHH
AGLSLDQVSLFELNEAFAVQSLVCIDELKLDPERVNVNGGAIAMGHPLGCTGTKLTATLL
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHHHH
HELRRRKERYGCVSMCIGGGMGAAGIFEMV
HHHHHHHHHHCHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure
MRTAYIVIALRTPATKAYKGKLRQVRPDDLAAAAIKGVIDATGIDPAELDDIIIGCAFPE
CCEEEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHCCEEEECCCCC
AEQGMNVARIAAFKAGVPYQVPCQTVNRFCSSGLQTIATAAERIMAGFADCIVAGGTESM
HHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCE
SLIPMGGNKFSANPGLVHAWPETYASMGITAELMVEKYGVSREDQDAFAAASHQKAAQAI
EEEEECCCEECCCCCEEEECHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
REGRFADEIVPVKAQICGLDGLKMKCSVEQVSVDDGVRADTTVAALAKLKPAFKANGSVT
HCCCCHHHEECCHHEEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCHHHCCCCCEE
AGNSSQMTDGAAAALVVSEDFLKRTGLQPMARFVCFAVKGVPPEIMGTGPIEAIPAALKL
CCCCCCCCCCHHEEEEECHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHH
AGLSLDQVSLFELNEAFAVQSLVCIDELKLDPERVNVNGGAIAMGHPLGCTGTKLTATLL
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHHHH
HELRRRKERYGCVSMCIGGGMGAAGIFEMV
HHHHHHHHHHCHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]