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The map label for this gene is nrfA-1 [H]

Identifier: 197118452

GI number: 197118452

Start: 2391436

End: 2392914

Strand: Direct

Name: nrfA-1 [H]

Synonym: Gbem_2070

Alternate gene names: 197118452

Gene position: 2391436-2392914 (Clockwise)

Preceding gene: 197118451

Following gene: 197118453

Centisome position: 51.82

GC content: 60.99

Gene sequence:

>1479_bases
ATGCGTCTCACCTATGCGAAGCGATGTGGTAGCGCGGTTTTGGTCCTTTTGGCAGCAACGGCACTTTGGACCGGATGTAC
CCATAACAAAGCGGAATCGAGGCCGCCGGCGGAGATGGCAGAGGAGCCGATGGATCCGGCCGATGCCGCCATCGACCCGG
CTCATTGGGGAAGGCTTTACCCGGTGCACTATCAGCAGTGGAAGCTCACCAGCGAACCGACTCCGGCCGGCTTGAGCAAG
TACAAACGCGGGTACGACGTGGGCGAAGAGCGCCGCGACAAGCTGGACGAGTACCCATTCCTCGCGCTTTTGTACAGCGG
ATGGGGGTTCGGCGCGGAATACAACGAACCGCGCGGGCATTACCACATGGTGCAGGACCAGCTTGAGGTGGATCCGGGCA
GGCTCAAGGCCGGGGGCGCCTGCCTCACCTGCAAAACCCCTTACGCGCCGAAGCTGCAAAAGGAGATGGGGAGAGCCTAC
TTCGCGACCCCCTACAAGGAAGTGCTGGCAAGGCTTCCCAAGCAGAACCAGACGCTCGGGGTGGCCTGCATCGACTGCCA
CGACAACCGCGGCATGGAGCTGAGAATTTCACGCGAGTTCACGCTGGGCAAGGCGCTCAGGAAGATCGGGGTGGACCCGG
CCAAACTCACTAGGGAGCAGAAGAGGACGCTTGTCTGCGCCCAGTGCCACGTTACCTACATGATCCCCAAGGACAAGGAT
ATGCATTCGACGGACGTGGTGTTTCCCTGGGCGGGTAGCAGGGAAGGGGGGATACCGATCGAGAACATCATCAAGGACAT
CAAGAGTTCCCCTTCGCACCTTGAGTGGACCCAGGCGGTAACCGGCTTCAAGCTCGCCTTTTTACGGCACCCCGAATACG
AGTTTTACTCCAACAACAGCCCCCACTGGTCCGCTGGCGTTACCTGCGCCGACTGCCATATGCCGATGATGACCGTCAGC
CACGAGAGCCTGACCGACCACAGGATCATGAGCCCGCTAAAGGGTGAGATGACGCAGTGCGCCGGTTGCCACGAGGAGAA
AGCGCCGGAGTTGCGCGCCAAGGTGATTGCGATGCAGGACCGCTTTATCGGCAGCTACCTGAAAACCGGGTATGCTGTCG
CAGCCGACGCCAAGCTTTTCGAGATGGCCAACAAGGCGAAAGCGGCAGGCAAGCAGATCGACAACGAGGTCTACGGCATA
GCCAGGGAGAATTACGAGCAGGCCTTCTACCGCCTCATCTTCGTCGGCGCCGAGAACTCCACCGGTTTCCACAACCCCAA
AGAGGGGCTGCGGATCTTGGACGACGCCGCGCGCCACGCGGCCACCGCCGACATGCACCTGCGCCAGATGCTGGCAAAGG
CGGGCGAGCGGGTCCCGGACCAGGTCGATCTTGAGCTTCCCAAGTACACCGGCAACCGCGGTACGAAGCATCTGCCGTTC
CGACCGGAACATGAGATCAAGGCGCCCATCGTGAAATAG

Upstream 100 bases:

>100_bases
GGAATTCCCTTGGATTAAACAGATGGTAGACTCGGAAAGTCTCGCCGGAGGCCTGCAAGGTCTGCGGAACCAAACGCCAA
CGGAAAAGGGGGGTATCGAC

Downstream 100 bases:

>100_bases
GGGCTCCCAAGCATTAAGATGCCTTCGCGGAACGCTCCCGCGAAGGCATCTTTCGTTTCAGGCGTCCCCCCCCCTCGGAA
TTGGCGGGGGACTGGCTCCG

Product: cytochrome c nitrite reductase catalytic subunit lipoprotein

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MRLTYAKRCGSAVLVLLAATALWTGCTHNKAESRPPAEMAEEPMDPADAAIDPAHWGRLYPVHYQQWKLTSEPTPAGLSK
YKRGYDVGEERRDKLDEYPFLALLYSGWGFGAEYNEPRGHYHMVQDQLEVDPGRLKAGGACLTCKTPYAPKLQKEMGRAY
FATPYKEVLARLPKQNQTLGVACIDCHDNRGMELRISREFTLGKALRKIGVDPAKLTREQKRTLVCAQCHVTYMIPKDKD
MHSTDVVFPWAGSREGGIPIENIIKDIKSSPSHLEWTQAVTGFKLAFLRHPEYEFYSNNSPHWSAGVTCADCHMPMMTVS
HESLTDHRIMSPLKGEMTQCAGCHEEKAPELRAKVIAMQDRFIGSYLKTGYAVAADAKLFEMANKAKAAGKQIDNEVYGI
ARENYEQAFYRLIFVGAENSTGFHNPKEGLRILDDAARHAATADMHLRQMLAKAGERVPDQVDLELPKYTGNRGTKHLPF
RPEHEIKAPIVK

Sequences:

>Translated_492_residues
MRLTYAKRCGSAVLVLLAATALWTGCTHNKAESRPPAEMAEEPMDPADAAIDPAHWGRLYPVHYQQWKLTSEPTPAGLSK
YKRGYDVGEERRDKLDEYPFLALLYSGWGFGAEYNEPRGHYHMVQDQLEVDPGRLKAGGACLTCKTPYAPKLQKEMGRAY
FATPYKEVLARLPKQNQTLGVACIDCHDNRGMELRISREFTLGKALRKIGVDPAKLTREQKRTLVCAQCHVTYMIPKDKD
MHSTDVVFPWAGSREGGIPIENIIKDIKSSPSHLEWTQAVTGFKLAFLRHPEYEFYSNNSPHWSAGVTCADCHMPMMTVS
HESLTDHRIMSPLKGEMTQCAGCHEEKAPELRAKVIAMQDRFIGSYLKTGYAVAADAKLFEMANKAKAAGKQIDNEVYGI
ARENYEQAFYRLIFVGAENSTGFHNPKEGLRILDDAARHAATADMHLRQMLAKAGERVPDQVDLELPKYTGNRGTKHLPF
RPEHEIKAPIVK
>Mature_492_residues
MRLTYAKRCGSAVLVLLAATALWTGCTHNKAESRPPAEMAEEPMDPADAAIDPAHWGRLYPVHYQQWKLTSEPTPAGLSK
YKRGYDVGEERRDKLDEYPFLALLYSGWGFGAEYNEPRGHYHMVQDQLEVDPGRLKAGGACLTCKTPYAPKLQKEMGRAY
FATPYKEVLARLPKQNQTLGVACIDCHDNRGMELRISREFTLGKALRKIGVDPAKLTREQKRTLVCAQCHVTYMIPKDKD
MHSTDVVFPWAGSREGGIPIENIIKDIKSSPSHLEWTQAVTGFKLAFLRHPEYEFYSNNSPHWSAGVTCADCHMPMMTVS
HESLTDHRIMSPLKGEMTQCAGCHEEKAPELRAKVIAMQDRFIGSYLKTGYAVAADAKLFEMANKAKAAGKQIDNEVYGI
ARENYEQAFYRLIFVGAENSTGFHNPKEGLRILDDAARHAATADMHLRQMLAKAGERVPDQVDLELPKYTGNRGTKHLPF
RPEHEIKAPIVK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=442, Percent_Identity=29.8642533936652, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 55265; Mature: 55265

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTYAKRCGSAVLVLLAATALWTGCTHNKAESRPPAEMAEEPMDPADAAIDPAHWGRLY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHCCCCHHHCCCCC
PVHYQQWKLTSEPTPAGLSKYKRGYDVGEERRDKLDEYPFLALLYSGWGFGAEYNEPRGH
EEEEEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
YHMVQDQLEVDPGRLKAGGACLTCKTPYAPKLQKEMGRAYFATPYKEVLARLPKQNQTLG
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHCCEEECCCHHHHHHHCCCCCCEEE
VACIDCHDNRGMELRISREFTLGKALRKIGVDPAKLTREQKRTLVCAQCHVTYMIPKDKD
EEEEEECCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEEEEEECCCCCC
MHSTDVVFPWAGSREGGIPIENIIKDIKSSPSHLEWTQAVTGFKLAFLRHPEYEFYSNNS
CCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCC
PHWSAGVTCADCHMPMMTVSHESLTDHRIMSPLKGEMTQCAGCHEEKAPELRAKVIAMQD
CCCCCCCEEECCCCCEEEECCHHHHHHHHHCCHHHHHHHHCCCCHHCCHHHHHHHHHHHH
RFIGSYLKTGYAVAADAKLFEMANKAKAAGKQIDNEVYGIARENYEQAFYRLIFVGAENS
HHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCC
TGFHNPKEGLRILDDAARHAATADMHLRQMLAKAGERVPDQVDLELPKYTGNRGTKHLPF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCC
RPEHEIKAPIVK
CCCCCCCCCCCC
>Mature Secondary Structure
MRLTYAKRCGSAVLVLLAATALWTGCTHNKAESRPPAEMAEEPMDPADAAIDPAHWGRLY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHCCCCHHHCCCCC
PVHYQQWKLTSEPTPAGLSKYKRGYDVGEERRDKLDEYPFLALLYSGWGFGAEYNEPRGH
EEEEEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
YHMVQDQLEVDPGRLKAGGACLTCKTPYAPKLQKEMGRAYFATPYKEVLARLPKQNQTLG
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHCCEEECCCHHHHHHHCCCCCCEEE
VACIDCHDNRGMELRISREFTLGKALRKIGVDPAKLTREQKRTLVCAQCHVTYMIPKDKD
EEEEEECCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEEEEEECCCCCC
MHSTDVVFPWAGSREGGIPIENIIKDIKSSPSHLEWTQAVTGFKLAFLRHPEYEFYSNNS
CCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCC
PHWSAGVTCADCHMPMMTVSHESLTDHRIMSPLKGEMTQCAGCHEEKAPELRAKVIAMQD
CCCCCCCEEECCCCCEEEECCHHHHHHHHHCCHHHHHHHHCCCCHHCCHHHHHHHHHHHH
RFIGSYLKTGYAVAADAKLFEMANKAKAAGKQIDNEVYGIARENYEQAFYRLIFVGAENS
HHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCC
TGFHNPKEGLRILDDAARHAATADMHLRQMLAKAGERVPDQVDLELPKYTGNRGTKHLPF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCC
RPEHEIKAPIVK
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA