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The map label for this gene is mdeA [H]

Identifier: 197118364

GI number: 197118364

Start: 2284141

End: 2285394

Strand: Reverse

Name: mdeA [H]

Synonym: Gbem_1982

Alternate gene names: 197118364

Gene position: 2285394-2284141 (Counterclockwise)

Preceding gene: 197118395

Following gene: 197118363

Centisome position: 49.52

GC content: 62.84

Gene sequence:

>1254_bases
GTGGGAGAGAAAGAAAAGAAGCTGAGATTCGACACGAAACTGATCCACGGCGGCACCTCGCCCGGGCCCTCCGGCGCCAC
CAAGACCCCGATAGTACAGGCATCCGCCTTCGCCTACGACACGGCGGAAGAACTGGAAGACATCTTCAGGGGAAGAGCCG
TAGGCCAGGTGTACACGAGGATAGGCAACCCCACCGTGGACACCCTGGAAAAGAGGCTGGCCGCGATCGAGGACGGCATC
GCCGCCGTCGCCACCTCTTCGGGCATGGCGGCGATCACCACCTCGGTCATGGCCGTAGTCAGAAGCGGCGACGAGGTCCT
CTCCTCCTCCTCGCTTTTCGGGGGCACCTACTCGCTGTTCCACGTCACCCTGGCCAACTTCGGGATCGAAACCCGCTTCG
TCGACCCCGTCGACCTTGCCGCGGTCGAGGCGGGGATCAACGACAAAACCCGACTGATCTTCGTGGAAACCATCGGCAAC
CCGAAGATGGACGTTCCCGACATAGCGGCTTTCGCAGCCATCGCCAAAAAGCACGGCATCCCGCTCATGGTCGACGCCAC
CGTTTCCACGCCGTATCTCGCGCGATCCAAGGAACTCGGGGCCGACATCGTCGTCCATTCCACCAGCAAGTACATCAACG
GAACAGCCAACTCCATCGGCGGCGCCATCATAGATGCCGGGAGCTTCAACTGGCAGAGCCCGAAGTTCCCGCACTTCGAG
CAGTTTTTCCGCAAGTACCGCAGCTTCGCCTTCACGGCACGGGTCCGCAAGCTGATCCACAAGGATTTCGGCGCCTGCGC
CGCGCCGCTCAACTCGTTCCTTTTGGGCGAGGGGCTGGAGACGCTGGCCCTGCGCATGGAGCGGCACTGCTCCAACGCCC
TCCAGGTGGCCCGGTTTCTTCAGGCGCACGAAAAGGTCACTTGGGTCAACTACCCCGGCCTCGACGATTCCCCCTTTTAC
GAGGTGGCGAAGCGCCAGTTCAACGGCCGCTTCGGCGGGCTCTTGACCTTCGGGCTCACGGACAAGGCCGCCGCCTTCCG
GGTCATCAACAACCTGCGGCTGGCCAAGAATCTCGCCAACATCGGCGACACCAAGACTCTGGTGATCCACCCGGCGAGCA
CCATCTGCGCCGATTACACCCCCGAGGTGAAGGCGCTCATGGGAGTGAGCGAGGAGCAGGTCAGGGTCTCGGTGGGGATC
GAGGACATCGAGGACATCCTGGAGGATTTCGCGGCCGCGCTGGAAGAGGCCTGA

Upstream 100 bases:

>100_bases
TACTGTTCCACTCAACAGTGCTACAGTATTTGCCGACATGTCCGATTTGCCCTGGAATTCCCGTGGCATCGACATCTAAA
GATGGCATTTTGGAGGGGTT

Downstream 100 bases:

>100_bases
GGGAAAATCTGTTCTTTACCAATCATCTATGTTACTAAACAAGGAGCGATCTTAATGAATCTTACAGTCAACGGCAAACC
AGCCAGCATCGCAGAGGAGA

Product: O-acetyl-L-homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MGEKEKKLRFDTKLIHGGTSPGPSGATKTPIVQASAFAYDTAEELEDIFRGRAVGQVYTRIGNPTVDTLEKRLAAIEDGI
AAVATSSGMAAITTSVMAVVRSGDEVLSSSSLFGGTYSLFHVTLANFGIETRFVDPVDLAAVEAGINDKTRLIFVETIGN
PKMDVPDIAAFAAIAKKHGIPLMVDATVSTPYLARSKELGADIVVHSTSKYINGTANSIGGAIIDAGSFNWQSPKFPHFE
QFFRKYRSFAFTARVRKLIHKDFGACAAPLNSFLLGEGLETLALRMERHCSNALQVARFLQAHEKVTWVNYPGLDDSPFY
EVAKRQFNGRFGGLLTFGLTDKAAAFRVINNLRLAKNLANIGDTKTLVIHPASTICADYTPEVKALMGVSEEQVRVSVGI
EDIEDILEDFAAALEEA

Sequences:

>Translated_417_residues
MGEKEKKLRFDTKLIHGGTSPGPSGATKTPIVQASAFAYDTAEELEDIFRGRAVGQVYTRIGNPTVDTLEKRLAAIEDGI
AAVATSSGMAAITTSVMAVVRSGDEVLSSSSLFGGTYSLFHVTLANFGIETRFVDPVDLAAVEAGINDKTRLIFVETIGN
PKMDVPDIAAFAAIAKKHGIPLMVDATVSTPYLARSKELGADIVVHSTSKYINGTANSIGGAIIDAGSFNWQSPKFPHFE
QFFRKYRSFAFTARVRKLIHKDFGACAAPLNSFLLGEGLETLALRMERHCSNALQVARFLQAHEKVTWVNYPGLDDSPFY
EVAKRQFNGRFGGLLTFGLTDKAAAFRVINNLRLAKNLANIGDTKTLVIHPASTICADYTPEVKALMGVSEEQVRVSVGI
EDIEDILEDFAAALEEA
>Mature_416_residues
GEKEKKLRFDTKLIHGGTSPGPSGATKTPIVQASAFAYDTAEELEDIFRGRAVGQVYTRIGNPTVDTLEKRLAAIEDGIA
AVATSSGMAAITTSVMAVVRSGDEVLSSSSLFGGTYSLFHVTLANFGIETRFVDPVDLAAVEAGINDKTRLIFVETIGNP
KMDVPDIAAFAAIAKKHGIPLMVDATVSTPYLARSKELGADIVVHSTSKYINGTANSIGGAIIDAGSFNWQSPKFPHFEQ
FFRKYRSFAFTARVRKLIHKDFGACAAPLNSFLLGEGLETLALRMERHCSNALQVARFLQAHEKVTWVNYPGLDDSPFYE
VAKRQFNGRFGGLLTFGLTDKAAAFRVINNLRLAKNLANIGDTKTLVIHPASTICADYTPEVKALMGVSEEQVRVSVGIE
DIEDILEDFAAALEEA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=397, Percent_Identity=32.9974811083123, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI299473759, Length=397, Percent_Identity=30.9823677581864, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI25453487, Length=396, Percent_Identity=28.2828282828283, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1790375, Length=355, Percent_Identity=33.8028169014084, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789383, Length=421, Percent_Identity=26.3657957244656, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71980708, Length=423, Percent_Identity=33.096926713948, Blast_Score=215, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17538055, Length=401, Percent_Identity=32.6683291770574, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=399, Percent_Identity=31.5789473684211, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=40.1847575057737, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6319307, Length=416, Percent_Identity=31.0096153846154, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=309, Percent_Identity=29.126213592233, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=419, Percent_Identity=25.5369928400955, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322970, Length=422, Percent_Identity=24.1706161137441, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922554, Length=415, Percent_Identity=31.8072289156626, Blast_Score=194, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45075; Mature: 44944

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00359 RIBOSOMAL_L11

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKEKKLRFDTKLIHGGTSPGPSGATKTPIVQASAFAYDTAEELEDIFRGRAVGQVYTR
CCCHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHCCHHHHHHHH
IGNPTVDTLEKRLAAIEDGIAAVATSSGMAAITTSVMAVVRSGDEVLSSSSLFGGTYSLF
CCCCCHHHHHHHHHHHHCCHHHEECCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHH
HVTLANFGIETRFVDPVDLAAVEAGINDKTRLIFVETIGNPKMDVPDIAAFAAIAKKHGI
HHHHHHCCCCEEECCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCC
PLMVDATVSTPYLARSKELGADIVVHSTSKYINGTANSIGGAIIDAGSFNWQSPKFPHFE
CEEEECCCCCCHHHHHHHCCCEEEEECCHHHCCCCCHHCCCEEEECCCCCCCCCCCCCHH
QFFRKYRSFAFTARVRKLIHKDFGACAAPLNSFLLGEGLETLALRMERHCSNALQVARFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QAHEKVTWVNYPGLDDSPFYEVAKRQFNGRFGGLLTFGLTDKAAAFRVINNLRLAKNLAN
HHHCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
IGDTKTLVIHPASTICADYTPEVKALMGVSEEQVRVSVGIEDIEDILEDFAAALEEA
CCCCCEEEEECCHHHHCCCCCHHHHHHCCCHHHEEEEECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GEKEKKLRFDTKLIHGGTSPGPSGATKTPIVQASAFAYDTAEELEDIFRGRAVGQVYTR
CCHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHCCHHHHHHHH
IGNPTVDTLEKRLAAIEDGIAAVATSSGMAAITTSVMAVVRSGDEVLSSSSLFGGTYSLF
CCCCCHHHHHHHHHHHHCCHHHEECCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHH
HVTLANFGIETRFVDPVDLAAVEAGINDKTRLIFVETIGNPKMDVPDIAAFAAIAKKHGI
HHHHHHCCCCEEECCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCC
PLMVDATVSTPYLARSKELGADIVVHSTSKYINGTANSIGGAIIDAGSFNWQSPKFPHFE
CEEEECCCCCCHHHHHHHCCCEEEEECCHHHCCCCCHHCCCEEEECCCCCCCCCCCCCHH
QFFRKYRSFAFTARVRKLIHKDFGACAAPLNSFLLGEGLETLALRMERHCSNALQVARFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QAHEKVTWVNYPGLDDSPFYEVAKRQFNGRFGGLLTFGLTDKAAAFRVINNLRLAKNLAN
HHHCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
IGDTKTLVIHPASTICADYTPEVKALMGVSEEQVRVSVGIEDIEDILEDFAAALEEA
CCCCCEEEEECCHHHHCCCCCHHHHHHCCCHHHEEEEECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]