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The map label for this gene is thiF [H]

Identifier: 197118362

GI number: 197118362

Start: 2283062

End: 2283874

Strand: Reverse

Name: thiF [H]

Synonym: Gbem_1980

Alternate gene names: 197118362

Gene position: 2283874-2283062 (Counterclockwise)

Preceding gene: 197118363

Following gene: 197118361

Centisome position: 49.49

GC content: 64.45

Gene sequence:

>813_bases
GTGAAGCTCAGCGAAGAACAGATCACCCGCTATTCCCGGCACATCCTGTTGAAGGAAGTCGGCGGTAAGGGGCAGAAGAA
GCTGCTTGAGGGGCGCGTTCTCATCATCGGGGCTGGCGGCCTCGGTTCCCCGATTGCCCTGTACCTGGCGGCAGCCGGTG
TCGGCACCATCGGCATCGCGGACGCCGACGAGGTGGACCTCTCCAACCTGCAGCGCCAGGTCATCCACACCACCCCTGAC
GTGGGGAAGGCGAAGGTGCTCTCGGCCAAGGAAACGATGCTGGCGATCAACCCCGAGCTCACCGTGAACGCCTACCAGAC
CTGGGTTTCCGCCGAGAACATCGCCGGCCTCATCGCCGACTACGACTTCGTCATCGACGGCACCGACAACTTCGCGGCCA
AGTTCCTCATCAACGACGCCTGCGTCCTCGCCGGCAAGCCCTACTCCCACGGCGGGATACTGCAGTTCGACGGGCAGACC
ATGACGGTCAAGCCGGGGGAATCCCCCTGCTACCGCTGCATCTTCCCGGAGCCTCCCCCGAAGGACGTGATCCCGACCTG
CTCGCGGGCAGGGGTCATCGGCGTCCTCCCCGGCGTGCTCGGCACGCTTCAGGCCACCGAGGCGATCAAGTACCTGCTGG
GAGCCGGCGATCTTTTGACCGGGCGCCTGCTTACCTACAACGCTCTCAGGATGCGCTTCAGGGAAATCCCGGTGAAGAAG
AGCGCCAAGTGCCCGATCTGCGGCGACCACCCCACCATCACCGAGCTGAAGGACGAGCTTGACGCCATGACCGTCTGCGA
CCTGGGACACTGA

Upstream 100 bases:

>100_bases
AACTGAACGGCGACATCCTCGACCGCGGCGATTTCGACGGCGCAACGGTGAAGAACGGCGACAACGTGGAATTTCTCTAT
TTCATGGGTGGAGGTAGATA

Downstream 100 bases:

>100_bases
TATGGTCGAGATACCGAAAGAGATACTGGCCGCGGTGATAAGCCACGCCCAGAACGGGTTTCCGCTGGAGGTCTGCGGCA
TACTGGGCGGCAACGGACAG

Product: thiamin biosynthesis thiocarboxylate synthase

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKLSEEQITRYSRHILLKEVGGKGQKKLLEGRVLIIGAGGLGSPIALYLAAAGVGTIGIADADEVDLSNLQRQVIHTTPD
VGKAKVLSAKETMLAINPELTVNAYQTWVSAENIAGLIADYDFVIDGTDNFAAKFLINDACVLAGKPYSHGGILQFDGQT
MTVKPGESPCYRCIFPEPPPKDVIPTCSRAGVIGVLPGVLGTLQATEAIKYLLGAGDLLTGRLLTYNALRMRFREIPVKK
SAKCPICGDHPTITELKDELDAMTVCDLGH

Sequences:

>Translated_270_residues
MKLSEEQITRYSRHILLKEVGGKGQKKLLEGRVLIIGAGGLGSPIALYLAAAGVGTIGIADADEVDLSNLQRQVIHTTPD
VGKAKVLSAKETMLAINPELTVNAYQTWVSAENIAGLIADYDFVIDGTDNFAAKFLINDACVLAGKPYSHGGILQFDGQT
MTVKPGESPCYRCIFPEPPPKDVIPTCSRAGVIGVLPGVLGTLQATEAIKYLLGAGDLLTGRLLTYNALRMRFREIPVKK
SAKCPICGDHPTITELKDELDAMTVCDLGH
>Mature_270_residues
MKLSEEQITRYSRHILLKEVGGKGQKKLLEGRVLIIGAGGLGSPIALYLAAAGVGTIGIADADEVDLSNLQRQVIHTTPD
VGKAKVLSAKETMLAINPELTVNAYQTWVSAENIAGLIADYDFVIDGTDNFAAKFLINDACVLAGKPYSHGGILQFDGQT
MTVKPGESPCYRCIFPEPPPKDVIPTCSRAGVIGVLPGVLGTLQATEAIKYLLGAGDLLTGRLLTYNALRMRFREIPVKK
SAKCPICGDHPTITELKDELDAMTVCDLGH

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=257, Percent_Identity=43.579766536965, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4885649, Length=159, Percent_Identity=32.7044025157233, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI13376212, Length=265, Percent_Identity=29.811320754717, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI38327032, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI23510338, Length=194, Percent_Identity=27.8350515463918, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI23510340, Length=194, Percent_Identity=27.8350515463918, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI38045942, Length=173, Percent_Identity=27.7456647398844, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI38045944, Length=173, Percent_Identity=27.7456647398844, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI150417996, Length=195, Percent_Identity=26.1538461538462, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787048, Length=252, Percent_Identity=40.4761904761905, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=41.3654618473896, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789177, Length=115, Percent_Identity=33.0434782608696, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=254, Percent_Identity=42.1259842519685, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI193203301, Length=198, Percent_Identity=28.2828282828283, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71984476, Length=183, Percent_Identity=29.5081967213115, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=259, Percent_Identity=36.6795366795367, Blast_Score=170, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320598, Length=171, Percent_Identity=30.4093567251462, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322639, Length=191, Percent_Identity=30.3664921465969, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325323, Length=173, Percent_Identity=29.4797687861272, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582879, Length=256, Percent_Identity=42.96875, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24660640, Length=168, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28573937, Length=188, Percent_Identity=29.7872340425532, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24641311, Length=255, Percent_Identity=29.8039215686275, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20129973, Length=174, Percent_Identity=28.735632183908, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 28861; Mature: 28861

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSEEQITRYSRHILLKEVGGKGQKKLLEGRVLIIGAGGLGSPIALYLAAAGVGTIGIA
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCEEECC
DADEVDLSNLQRQVIHTTPDVGKAKVLSAKETMLAINPELTVNAYQTWVSAENIAGLIAD
CCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHEEEC
YDFVIDGTDNFAAKFLINDACVLAGKPYSHGGILQFDGQTMTVKPGESPCYRCIFPEPPP
EEEEEECCCCCEEEEEECCEEEEECCCCCCCCEEEECCEEEEECCCCCCCEEEECCCCCC
KDVIPTCSRAGVIGVLPGVLGTLQATEAIKYLLGAGDLLTGRLLTYNALRMRFREIPVKK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
SAKCPICGDHPTITELKDELDAMTVCDLGH
CCCCCCCCCCCCHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MKLSEEQITRYSRHILLKEVGGKGQKKLLEGRVLIIGAGGLGSPIALYLAAAGVGTIGIA
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCEEECC
DADEVDLSNLQRQVIHTTPDVGKAKVLSAKETMLAINPELTVNAYQTWVSAENIAGLIAD
CCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHEEEC
YDFVIDGTDNFAAKFLINDACVLAGKPYSHGGILQFDGQTMTVKPGESPCYRCIFPEPPP
EEEEEECCCCCEEEEEECCEEEEECCCCCCCCEEEECCEEEEECCCCCCCEEEECCCCCC
KDVIPTCSRAGVIGVLPGVLGTLQATEAIKYLLGAGDLLTGRLLTYNALRMRFREIPVKK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
SAKCPICGDHPTITELKDELDAMTVCDLGH
CCCCCCCCCCCCHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]