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The map label for this gene is vapI [H]

Identifier: 197118289

GI number: 197118289

Start: 2208046

End: 2208348

Strand: Reverse

Name: vapI [H]

Synonym: Gbem_1906

Alternate gene names: 197118289

Gene position: 2208348-2208046 (Counterclockwise)

Preceding gene: 197118290

Following gene: 197118288

Centisome position: 47.85

GC content: 48.84

Gene sequence:

>303_bases
ATGTCCTATACAAATAATCTGCCTCCCATTCATCCTGGAGTATTCTTAAAAGAAATACTTGATGACCTTACCGTCACGCA
GACTGCTTTTGCCAAAGCTATCGGCGTTTCTCCTATGCGAGTTTCGCACGTCATCAAGGGTACACGCCCTGTGACTGCGG
AGTTGGCCTTACTCTTCGGCAAAGCCTTTAGTCAGACGCCGACGTATTGGATGAATCTGCAAACCAGCTTCGATCTGAAA
ACAGCAGAAAAGGAACTCGCACAAAAGGTGCGCCAAGTACATCCGCTCTCGCAGGCAGCGTAG

Upstream 100 bases:

>100_bases
TGGAGTATAAGAATCAACGACCAGTGGCGAATTTGTTTTTCCTTCGCAGATGGTGACGCCACCCTAGTCGAGATAACCGA
CTATCACTGAGGAGACCCTT

Downstream 100 bases:

>100_bases
CCGACTATCTTCTTCAAAAAGGCAAAAGGCGGAACACAGAGGGCACTGAGGTCCACGGAGGTCACCGAGGTTTTCAGGTA
AAGACAACAGAAAAACAGAT

Product: antitoxin VapI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 100; Mature: 99

Protein sequence:

>100_residues
MSYTNNLPPIHPGVFLKEILDDLTVTQTAFAKAIGVSPMRVSHVIKGTRPVTAELALLFGKAFSQTPTYWMNLQTSFDLK
TAEKELAQKVRQVHPLSQAA

Sequences:

>Translated_100_residues
MSYTNNLPPIHPGVFLKEILDDLTVTQTAFAKAIGVSPMRVSHVIKGTRPVTAELALLFGKAFSQTPTYWMNLQTSFDLK
TAEKELAQKVRQVHPLSQAA
>Mature_99_residues
SYTNNLPPIHPGVFLKEILDDLTVTQTAFAKAIGVSPMRVSHVIKGTRPVTAELALLFGKAFSQTPTYWMNLQTSFDLKT
AEKELAQKVRQVHPLSQAA

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 11053; Mature: 10922

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTNNLPPIHPGVFLKEILDDLTVTQTAFAKAIGVSPMRVSHVIKGTRPVTAELALLFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHH
KAFSQTPTYWMNLQTSFDLKTAEKELAQKVRQVHPLSQAA
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SYTNNLPPIHPGVFLKEILDDLTVTQTAFAKAIGVSPMRVSHVIKGTRPVTAELALLFG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHH
KAFSQTPTYWMNLQTSFDLKTAEKELAQKVRQVHPLSQAA
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]