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The map label for this gene is pstS-2 [H]

Identifier: 197118230

GI number: 197118230

Start: 2140840

End: 2141892

Strand: Reverse

Name: pstS-2 [H]

Synonym: Gbem_1847

Alternate gene names: 197118230

Gene position: 2141892-2140840 (Counterclockwise)

Preceding gene: 197118231

Following gene: 197118229

Centisome position: 46.41

GC content: 60.97

Gene sequence:

>1053_bases
ATGTGGAACAGACTTGTCAAAAACATCCTCGCATTAGCCCTAGCCATCTCGGCCGCATCCGGTTCGGCCTACGCTGAAAC
TGCGCTCACCGGGGCCGGCGCCACTTTCCCCCATCCCCTCTACAGCAAGTGGTTCAGGGACTATCAGGCAGAAGATCCCG
GCGTGACCTTCCGCTACGACGCGCAGGGGAGCGGCGAGGGTATCAGGAGGATCCTGGCCCGCGAGGTCGACTTCGGCGCA
TCCGACAAGTTTCTCTCCGACGAAGAACTGAATAAAGCGCCCGGCAAGCTGCTGCACATCCCTACCGTGATGGGCGCCGT
CGCCGTCACCTACAATATCCCCGGAATCGGTTCCGACCTGAAACTCACGCCGCAGGCGCTTGCCGGGATCTACCTCGGGA
AGGTCACCAAATGGAACGACCCGCTCATCGCCAGGGAGAACGCCAAGCTCAAGCTCCCCGCTGAGGATATCGTCGCCGTG
CACCGCTCAGACGCCAGCGGCACCACTAGCATCTTTACCGACTACCTGAGCTCGGTGGACCCATCCTGGTCAAGCGGGAT
CGGCAAGGGAACGACCGTGGCCTGGCCTGCGGGAATCGGGGCGAAGGGGAGCAGTGCGGTGGTGAAGAAGATAAAGGAGG
TTCCCTACAGCATCGGCTACGTGGAGATCGCCTACGCGCTGGAGAACGAGCTGAATGCGGCGGCGCTGAAGAACCGGGCC
GGTAAGTTCGTCAGGCCGACCATGCTCTCCACGAGAGCCGCGGCCGTTCAGGGAATGAAAAATCACAAGATGAAAAAGGA
TGTGGAATTGGACTACCGCCTGTCCCTGGTGAACCAGCCGGGCAAGGACGCCTACCCTATCGTCGGGCTCACCTGGCTGC
TGGTGTACCGTGACCAGCAGGACCCGGTCAAGGGGAGGAAGCTGGTCGAGTTCCTTACCTGGCAGCTGAAAAAGGCCGAG
AAGATGACTTCCACGCTGCACTACACCCCGCTCCCCGACACCTGGTCCGCCCAGGTGGAAAAAACCATCAGGAGCATCGC
TGTACCGCAGTAA

Upstream 100 bases:

>100_bases
GCACATTTTCACACAATACGATCAATGTGGTAACAAAACCGTCACACTGAGCTGCTATGTTTCCTCCGTCAGAATGAAGG
GAAAAAGGAGACAGCACATC

Downstream 100 bases:

>100_bases
TCGAAAGATGAAAATGCCACGGCAATCGCCGTGGCATTTTCGTTACCGCTCCTATTTAGTTTCCAGCAGGGGACGAGCAG
TTTTGAGCCGCGCAGAGATT

Product: phosphate ABC transporter periplasmic phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MWNRLVKNILALALAISAASGSAYAETALTGAGATFPHPLYSKWFRDYQAEDPGVTFRYDAQGSGEGIRRILAREVDFGA
SDKFLSDEELNKAPGKLLHIPTVMGAVAVTYNIPGIGSDLKLTPQALAGIYLGKVTKWNDPLIARENAKLKLPAEDIVAV
HRSDASGTTSIFTDYLSSVDPSWSSGIGKGTTVAWPAGIGAKGSSAVVKKIKEVPYSIGYVEIAYALENELNAAALKNRA
GKFVRPTMLSTRAAAVQGMKNHKMKKDVELDYRLSLVNQPGKDAYPIVGLTWLLVYRDQQDPVKGRKLVEFLTWQLKKAE
KMTSTLHYTPLPDTWSAQVEKTIRSIAVPQ

Sequences:

>Translated_350_residues
MWNRLVKNILALALAISAASGSAYAETALTGAGATFPHPLYSKWFRDYQAEDPGVTFRYDAQGSGEGIRRILAREVDFGA
SDKFLSDEELNKAPGKLLHIPTVMGAVAVTYNIPGIGSDLKLTPQALAGIYLGKVTKWNDPLIARENAKLKLPAEDIVAV
HRSDASGTTSIFTDYLSSVDPSWSSGIGKGTTVAWPAGIGAKGSSAVVKKIKEVPYSIGYVEIAYALENELNAAALKNRA
GKFVRPTMLSTRAAAVQGMKNHKMKKDVELDYRLSLVNQPGKDAYPIVGLTWLLVYRDQQDPVKGRKLVEFLTWQLKKAE
KMTSTLHYTPLPDTWSAQVEKTIRSIAVPQ
>Mature_350_residues
MWNRLVKNILALALAISAASGSAYAETALTGAGATFPHPLYSKWFRDYQAEDPGVTFRYDAQGSGEGIRRILAREVDFGA
SDKFLSDEELNKAPGKLLHIPTVMGAVAVTYNIPGIGSDLKLTPQALAGIYLGKVTKWNDPLIARENAKLKLPAEDIVAV
HRSDASGTTSIFTDYLSSVDPSWSSGIGKGTTVAWPAGIGAKGSSAVVKKIKEVPYSIGYVEIAYALENELNAAALKNRA
GKFVRPTMLSTRAAAVQGMKNHKMKKDVELDYRLSLVNQPGKDAYPIVGLTWLLVYRDQQDPVKGRKLVEFLTWQLKKAE
KMTSTLHYTPLPDTWSAQVEKTIRSIAVPQ

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=325, Percent_Identity=41.8461538461538, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38138; Mature: 38138

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNRLVKNILALALAISAASGSAYAETALTGAGATFPHPLYSKWFRDYQAEDPGVTFRYD
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
AQGSGEGIRRILAREVDFGASDKFLSDEELNKAPGKLLHIPTVMGAVAVTYNIPGIGSDL
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEHHHHHHHEEEEEECCCCCCCC
KLTPQALAGIYLGKVTKWNDPLIARENAKLKLPAEDIVAVHRSDASGTTSIFTDYLSSVD
EECHHHHHHHHHHCCCCCCCCEEECCCCEEECCHHHHHEEECCCCCCHHHHHHHHHHHCC
PSWSSGIGKGTTVAWPAGIGAKGSSAVVKKIKEVPYSIGYVEIAYALENELNAAALKNRA
CCHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHCHHHHHHHHHC
GKFVRPTMLSTRAAAVQGMKNHKMKKDVELDYRLSLVNQPGKDAYPIVGLTWLLVYRDQQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHEEEEEEEECCCC
DPVKGRKLVEFLTWQLKKAEKMTSTLHYTPLPDTWSAQVEKTIRSIAVPQ
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MWNRLVKNILALALAISAASGSAYAETALTGAGATFPHPLYSKWFRDYQAEDPGVTFRYD
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
AQGSGEGIRRILAREVDFGASDKFLSDEELNKAPGKLLHIPTVMGAVAVTYNIPGIGSDL
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEHHHHHHHEEEEEECCCCCCCC
KLTPQALAGIYLGKVTKWNDPLIARENAKLKLPAEDIVAVHRSDASGTTSIFTDYLSSVD
EECHHHHHHHHHHCCCCCCCCEEECCCCEEECCHHHHHEEECCCCCCHHHHHHHHHHHCC
PSWSSGIGKGTTVAWPAGIGAKGSSAVVKKIKEVPYSIGYVEIAYALENELNAAALKNRA
CCHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHCHHHHHHHHHC
GKFVRPTMLSTRAAAVQGMKNHKMKKDVELDYRLSLVNQPGKDAYPIVGLTWLLVYRDQQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHEEEEEEEECCCC
DPVKGRKLVEFLTWQLKKAEKMTSTLHYTPLPDTWSAQVEKTIRSIAVPQ
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6321434; 6365894; 7686882; 9278503; 9298646; 2215649; 9228942; 9671506 [H]