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The map label for this gene is pstS-1 [H]

Identifier: 197118101

GI number: 197118101

Start: 1981654

End: 1982706

Strand: Reverse

Name: pstS-1 [H]

Synonym: Gbem_1716

Alternate gene names: 197118101

Gene position: 1982706-1981654 (Counterclockwise)

Preceding gene: 197118102

Following gene: 197118094

Centisome position: 42.96

GC content: 59.92

Gene sequence:

>1053_bases
ATGTTGAGACATCTCGCGAAGACCCTGGCACTGGTTGTTGCATTATCGGTAGGCGCCGGCGCGGCATACGCCGACACAAC
CCTCAAGGGAGCAGGCGCTACCTTCCCCCACCCCCTCTACAGCAAATGGTTCCGGGACTACCAGGCTGAGGACCCCTCCG
TAACTTTCAGCTATGAAGCCAAGGGAAGCGGACATGGGATAAAGAGGATAATAAGCGGCGAGGTCGACTTCGGCGCCACT
GACAAATTCCTCACCGACCAGGAACTGAAGGGGGCGCCCGGGAAACTGCTGCATGTCCCTACTGTCATAGGCGCCGTAGC
GGTGGCGTACAACATCCCCGGCATAGGCGCCGGGCTCAAACTGACACCGCAGGCCCTGGCGGGAATCTACCAAGGGAAAA
TCACCAAGTGGAACGCCCCTCTCCTTGCCAAGCTGAACCCGAAGCTGAGGCTTCCAGCCCAGGAGATCGTCGTCGTCCAC
CGCTCCGACAGCAGCGGCACCACCAGCATCTTCACCGACTACCTGAGCGCAGTGGACAAATCCTGGGCTACGTCCGTAGG
CAAAGGAACCAGCGTTGCCTGGCCGGTCGGGGAAGGAGCCGAGGGAAGCAGCGCGCTGGTCAAGAAGATCGGAGAGACGC
CCAACAGCATCGGGTACGTCGAGATAGCCTACGCCCTGGAAAACGACCTCAACACCGCGGCCCTCAAGAACAAAAGCGGC
AAGTTCGTCAAAGCGAACGTGTTGTCCACCCGCGCAGCGGCAGTGCAGGCGATGAAAAAACAGACGGGGAAAGCACTGTC
GGCTGAGCCCGATTTCCGTTTGTCACTGGTGGACCAACCGGGCAAGGAGTCCTACCCGGTCGTGGGTCTTACCTGGCTAT
TGGTGTACCAGGAGCAGCGGGACGCGGTACAGGGGAAAAAGCTGGTGGAGTTCCTGAGATGGCAGTTGAGAAAGGCGGAG
AAGATGACCTCGACGCTACACTACACGCCGCTCCCCGACACCTGGACGAGGCAGGTTGAGCAGACAATCGACAGCATCCG
TGTACCGCAGTAG

Upstream 100 bases:

>100_bases
CCCGGCGCTCTTTGTCACAAAAACAGGTCAAGCCCGTAACGAAGATGACACAGTGCACTGTTATCGTGGCCACGGATTCC
AACAACAAAGGAGACATGAC

Downstream 100 bases:

>100_bases
ATCATAAGACTTACTGAAAGTGCAGAAGGCGACGTCACAGCAGACGTCGCCTTTTTTTATTGAGTTCAAAGGGCAAGATC
CTGCTGCCCGATGACCGAAG

Product: phosphate ABC transporter periplasmic phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLRHLAKTLALVVALSVGAGAAYADTTLKGAGATFPHPLYSKWFRDYQAEDPSVTFSYEAKGSGHGIKRIISGEVDFGAT
DKFLTDQELKGAPGKLLHVPTVIGAVAVAYNIPGIGAGLKLTPQALAGIYQGKITKWNAPLLAKLNPKLRLPAQEIVVVH
RSDSSGTTSIFTDYLSAVDKSWATSVGKGTSVAWPVGEGAEGSSALVKKIGETPNSIGYVEIAYALENDLNTAALKNKSG
KFVKANVLSTRAAAVQAMKKQTGKALSAEPDFRLSLVDQPGKESYPVVGLTWLLVYQEQRDAVQGKKLVEFLRWQLRKAE
KMTSTLHYTPLPDTWTRQVEQTIDSIRVPQ

Sequences:

>Translated_350_residues
MLRHLAKTLALVVALSVGAGAAYADTTLKGAGATFPHPLYSKWFRDYQAEDPSVTFSYEAKGSGHGIKRIISGEVDFGAT
DKFLTDQELKGAPGKLLHVPTVIGAVAVAYNIPGIGAGLKLTPQALAGIYQGKITKWNAPLLAKLNPKLRLPAQEIVVVH
RSDSSGTTSIFTDYLSAVDKSWATSVGKGTSVAWPVGEGAEGSSALVKKIGETPNSIGYVEIAYALENDLNTAALKNKSG
KFVKANVLSTRAAAVQAMKKQTGKALSAEPDFRLSLVDQPGKESYPVVGLTWLLVYQEQRDAVQGKKLVEFLRWQLRKAE
KMTSTLHYTPLPDTWTRQVEQTIDSIRVPQ
>Mature_350_residues
MLRHLAKTLALVVALSVGAGAAYADTTLKGAGATFPHPLYSKWFRDYQAEDPSVTFSYEAKGSGHGIKRIISGEVDFGAT
DKFLTDQELKGAPGKLLHVPTVIGAVAVAYNIPGIGAGLKLTPQALAGIYQGKITKWNAPLLAKLNPKLRLPAQEIVVVH
RSDSSGTTSIFTDYLSAVDKSWATSVGKGTSVAWPVGEGAEGSSALVKKIGETPNSIGYVEIAYALENDLNTAALKNKSG
KFVKANVLSTRAAAVQAMKKQTGKALSAEPDFRLSLVDQPGKESYPVVGLTWLLVYQEQRDAVQGKKLVEFLRWQLRKAE
KMTSTLHYTPLPDTWTRQVEQTIDSIRVPQ

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=326, Percent_Identity=39.8773006134969, Blast_Score=236, Evalue=1e-63,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37707; Mature: 37707

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRHLAKTLALVVALSVGAGAAYADTTLKGAGATFPHPLYSKWFRDYQAEDPSVTFSYEA
CHHHHHHHHHHHHHHHCCCCCCHHCCEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
KGSGHGIKRIISGEVDFGATDKFLTDQELKGAPGKLLHVPTVIGAVAVAYNIPGIGAGLK
CCCCCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCE
LTPQALAGIYQGKITKWNAPLLAKLNPKLRLPAQEIVVVHRSDSSGTTSIFTDYLSAVDK
ECHHHHHHHHHCCEEECCCCEEEECCCCEECCHHHEEEEECCCCCCCHHHHHHHHHHHHH
SWATSVGKGTSVAWPVGEGAEGSSALVKKIGETPNSIGYVEIAYALENDLNTAALKNKSG
HHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHCCCCC
KFVKANVLSTRAAAVQAMKKQTGKALSAEPDFRLSLVDQPGKESYPVVGLTWLLVYQEQR
CEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEEEHHHH
DAVQGKKLVEFLRWQLRKAEKMTSTLHYTPLPDTWTRQVEQTIDSIRVPQ
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRHLAKTLALVVALSVGAGAAYADTTLKGAGATFPHPLYSKWFRDYQAEDPSVTFSYEA
CHHHHHHHHHHHHHHHCCCCCCHHCCEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
KGSGHGIKRIISGEVDFGATDKFLTDQELKGAPGKLLHVPTVIGAVAVAYNIPGIGAGLK
CCCCCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCE
LTPQALAGIYQGKITKWNAPLLAKLNPKLRLPAQEIVVVHRSDSSGTTSIFTDYLSAVDK
ECHHHHHHHHHCCEEECCCCEEEECCCCEECCHHHEEEEECCCCCCCHHHHHHHHHHHHH
SWATSVGKGTSVAWPVGEGAEGSSALVKKIGETPNSIGYVEIAYALENDLNTAALKNKSG
HHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHCCCCC
KFVKANVLSTRAAAVQAMKKQTGKALSAEPDFRLSLVDQPGKESYPVVGLTWLLVYQEQR
CEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEEEHHHH
DAVQGKKLVEFLRWQLRKAEKMTSTLHYTPLPDTWTRQVEQTIDSIRVPQ
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6321434; 6365894; 7686882; 9278503; 9298646; 2215649; 9228942; 9671506 [H]