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The map label for this gene is cysN-2 [H]

Identifier: 197118016

GI number: 197118016

Start: 1891064

End: 1892290

Strand: Reverse

Name: cysN-2 [H]

Synonym: Gbem_1629

Alternate gene names: 197118016

Gene position: 1892290-1891064 (Counterclockwise)

Preceding gene: 197118017

Following gene: 197118015

Centisome position: 41.0

GC content: 60.15

Gene sequence:

>1227_bases
ATGACAGATGCGATCAAATCGGCATTCCCCATAGCCATCACCGGCCATGTCGACCACGGCAAGAGCACCCTCATTGGAAG
GCTCCTCTACGACACCGGCACATTGCAGTCGGGCCGCTACCAAGAGATGCTGCAGAGCTCCTTGGAGACCGGGCGCGGTG
ACGAGTTCGCTTTCGTCCTCGACGCCTTCGAGGAGGAACGACGGCGCGGCATCACCATTGACACCTCGCAGATCTACTTC
AACTCAAAGCTTCGCCCCTACCTGATTATCGACACTCCGGGCCACCGCGAATTCATCCGCAACATGGTCACCGGTGCGAG
CTACGCCAAGGCGGCGGTGCTCATCGTCGACGCGGTGGAGGGCGTGATGGAGCAGACGCGGCGACATGCGTGGCTCCTGA
GCATCGTTGGGATTCAGGAAATCTGCGTTGCGGTCAACAAGATGGACGCAGTAGCCTACTCGAGCGACGCGTTCGCCGCC
CTTTCCGTCGCGGTGGAATCTCTTTTCACCGAGTTCGGCCTCTCCCCCGCAGCGATAGTGCCGATTTCCGCCCGCGTGGG
CGACAACGTGGCAAAGCTGAGCGGCTCGATGCCATGGTACACGGGAAAATCGCTGCTTGAAGTGCTGGACTCTCTGGAGT
GCCGCCCCATCGAAGAGCGTCCTTTCCGCTTCCCGGTCCAGGATGTCTACCGCTTCGATTCGGAGCCTATCGTGGTAGGC
AGAATAGAATCGGGAGCGGTACGGATTGGGGAGAAGGTCACCATTTACCCAGGCGGGCGCGAGTCCGCCATCGGCACCAT
CCGCACCTTCCCCTCCAGTGAAGCGGCCTCTGCGAGTTACGGAGAGGCTATCGGCTTCACTCTCGAAGCCGCAGATGCAG
AAATCGTACGCGGATCGGTCATTGCAGCAGGAGCTCCTCCAACCTGCGGTAGAGAGTTCCAGGCCACGGTGTTTTGGTTC
ATGGACGAATACGCGGCAGGAGACCCGGTCACCATCCGCTGCACCACACAGTCGGTGCCGGGCAGGATACTCCTACATCA
GGTCTTCGACCCGGCCCAACCGGACGCGGAACTTCCTTCCGACCTCATCTGCGTCGGCGAGGCAGCACGCTGTACCATAC
TCACCGAAGTAGACCTGGCAGGTGACCACCCTGGTCTCATCCCTGAAACAGGCCGCTTTGTCATTGAACGAGAAGGCATT
CCGGCAGGAGCAGGTATTTTTCGATGA

Upstream 100 bases:

>100_bases
CCGAGCTTGAAACTTCGACAGACTCCGAACGCGCCGGCAGGGCGCAGGACAAGGAGAACCTCTACACCATGCAGAAACTG
CGTTCGTTGGGATACATGTA

Downstream 100 bases:

>100_bases
GTAAGACGATTTTTCTCGGCATCGACGGCATGGACCCCAAGATTGCCGAGCAACTGATGAGTGCAGGCCAGCTCCCCAAC
TTCGAGCGGCTCGCAGCCCG

Product: sulfate adenylyltransferase subunit 1

Products: ADP; 3'-phosphoadenylyl sulfate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTDAIKSAFPIAITGHVDHGKSTLIGRLLYDTGTLQSGRYQEMLQSSLETGRGDEFAFVLDAFEEERRRGITIDTSQIYF
NSKLRPYLIIDTPGHREFIRNMVTGASYAKAAVLIVDAVEGVMEQTRRHAWLLSIVGIQEICVAVNKMDAVAYSSDAFAA
LSVAVESLFTEFGLSPAAIVPISARVGDNVAKLSGSMPWYTGKSLLEVLDSLECRPIEERPFRFPVQDVYRFDSEPIVVG
RIESGAVRIGEKVTIYPGGRESAIGTIRTFPSSEAASASYGEAIGFTLEAADAEIVRGSVIAAGAPPTCGREFQATVFWF
MDEYAAGDPVTIRCTTQSVPGRILLHQVFDPAQPDAELPSDLICVGEAARCTILTEVDLAGDHPGLIPETGRFVIEREGI
PAGAGIFR

Sequences:

>Translated_408_residues
MTDAIKSAFPIAITGHVDHGKSTLIGRLLYDTGTLQSGRYQEMLQSSLETGRGDEFAFVLDAFEEERRRGITIDTSQIYF
NSKLRPYLIIDTPGHREFIRNMVTGASYAKAAVLIVDAVEGVMEQTRRHAWLLSIVGIQEICVAVNKMDAVAYSSDAFAA
LSVAVESLFTEFGLSPAAIVPISARVGDNVAKLSGSMPWYTGKSLLEVLDSLECRPIEERPFRFPVQDVYRFDSEPIVVG
RIESGAVRIGEKVTIYPGGRESAIGTIRTFPSSEAASASYGEAIGFTLEAADAEIVRGSVIAAGAPPTCGREFQATVFWF
MDEYAAGDPVTIRCTTQSVPGRILLHQVFDPAQPDAELPSDLICVGEAARCTILTEVDLAGDHPGLIPETGRFVIEREGI
PAGAGIFR
>Mature_407_residues
TDAIKSAFPIAITGHVDHGKSTLIGRLLYDTGTLQSGRYQEMLQSSLETGRGDEFAFVLDAFEEERRRGITIDTSQIYFN
SKLRPYLIIDTPGHREFIRNMVTGASYAKAAVLIVDAVEGVMEQTRRHAWLLSIVGIQEICVAVNKMDAVAYSSDAFAAL
SVAVESLFTEFGLSPAAIVPISARVGDNVAKLSGSMPWYTGKSLLEVLDSLECRPIEERPFRFPVQDVYRFDSEPIVVGR
IESGAVRIGEKVTIYPGGRESAIGTIRTFPSSEAASASYGEAIGFTLEAADAEIVRGSVIAAGAPPTCGREFQATVFWFM
DEYAAGDPVTIRCTTQSVPGRILLHQVFDPAQPDAELPSDLICVGEAARCTILTEVDLAGDHPGLIPETGRFVIEREGIP
AGAGIFR

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503475, Length=367, Percent_Identity=35.6948228882834, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI4503471, Length=367, Percent_Identity=34.6049046321526, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI223555963, Length=424, Percent_Identity=30.8962264150943, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=424, Percent_Identity=30.8962264150943, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI194018522, Length=331, Percent_Identity=29.3051359516616, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194097354, Length=331, Percent_Identity=29.3051359516616, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194018520, Length=331, Percent_Identity=29.3051359516616, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI46094014, Length=329, Percent_Identity=29.483282674772, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI34147630, Length=333, Percent_Identity=29.4294294294294, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI157426893, Length=195, Percent_Identity=31.2820512820513, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI94966754, Length=211, Percent_Identity=28.9099526066351, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789108, Length=307, Percent_Identity=41.042345276873, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1790412, Length=323, Percent_Identity=30.030959752322, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789737, Length=323, Percent_Identity=30.030959752322, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI2367247, Length=300, Percent_Identity=26, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994988, Length=262, Percent_Identity=28.2442748091603, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788922, Length=263, Percent_Identity=27.3764258555133, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17552884, Length=338, Percent_Identity=36.3905325443787, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17569207, Length=338, Percent_Identity=36.3905325443787, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI115532067, Length=333, Percent_Identity=34.2342342342342, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI115532065, Length=333, Percent_Identity=34.2342342342342, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=331, Percent_Identity=34.1389728096677, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32566629, Length=333, Percent_Identity=29.1291291291291, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17556456, Length=419, Percent_Identity=26.491646778043, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=27.1794871794872, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=144, Percent_Identity=40.2777777777778, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=264, Percent_Identity=30.6818181818182, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17533571, Length=140, Percent_Identity=34.2857142857143, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325337, Length=422, Percent_Identity=31.7535545023697, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319594, Length=422, Percent_Identity=31.7535545023697, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322937, Length=333, Percent_Identity=30.3303303303303, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=417, Percent_Identity=29.0167865707434, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324761, Length=331, Percent_Identity=30.2114803625378, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=339, Percent_Identity=27.4336283185841, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322359, Length=168, Percent_Identity=32.1428571428571, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=179, Percent_Identity=29.608938547486, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652838, Length=419, Percent_Identity=31.5035799522673, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI17137572, Length=419, Percent_Identity=31.5035799522673, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI45553807, Length=338, Percent_Identity=34.6153846153846, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI45553816, Length=338, Percent_Identity=34.6153846153846, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24651721, Length=338, Percent_Identity=34.6153846153846, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI17864154, Length=338, Percent_Identity=34.6153846153846, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI45550900, Length=335, Percent_Identity=33.134328358209, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17137380, Length=415, Percent_Identity=26.5060240963855, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281363316, Length=249, Percent_Identity=32.1285140562249, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI17864358, Length=249, Percent_Identity=32.1285140562249, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19921738, Length=321, Percent_Identity=27.7258566978193, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI78706572, Length=188, Percent_Identity=32.4468085106383, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=154, Percent_Identity=35.0649350649351, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 2.7.1.25; 2.7.7.4

Molecular weight: Translated: 44163; Mature: 44031

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAIKSAFPIAITGHVDHGKSTLIGRLLYDTGTLQSGRYQEMLQSSLETGRGDEFAFVL
CCCCHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEH
DAFEEERRRGITIDTSQIYFNSKLRPYLIIDTPGHREFIRNMVTGASYAKAAVLIVDAVE
HHHHHHHHCCCEEECCEEEECCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHH
GVMEQTRRHAWLLSIVGIQEICVAVNKMDAVAYSSDAFAALSVAVESLFTEFGLSPAAIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCEEEE
PISARVGDNVAKLSGSMPWYTGKSLLEVLDSLECRPIEERPFRFPVQDVYRFDSEPIVVG
EEEHHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCEEEE
RIESGAVRIGEKVTIYPGGRESAIGTIRTFPSSEAASASYGEAIGFTLEAADAEIVRGSV
EECCCCEEECCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEECCEE
IAAGAPPTCGREFQATVFWFMDEYAAGDPVTIRCTTQSVPGRILLHQVFDPAQPDAELPS
EECCCCCCCCCCCEEEEEEEEHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCC
DLICVGEAARCTILTEVDLAGDHPGLIPETGRFVIEREGIPAGAGIFR
CEEEECCCCEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TDAIKSAFPIAITGHVDHGKSTLIGRLLYDTGTLQSGRYQEMLQSSLETGRGDEFAFVL
CCCHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEH
DAFEEERRRGITIDTSQIYFNSKLRPYLIIDTPGHREFIRNMVTGASYAKAAVLIVDAVE
HHHHHHHHCCCEEECCEEEECCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHH
GVMEQTRRHAWLLSIVGIQEICVAVNKMDAVAYSSDAFAALSVAVESLFTEFGLSPAAIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCEEEE
PISARVGDNVAKLSGSMPWYTGKSLLEVLDSLECRPIEERPFRFPVQDVYRFDSEPIVVG
EEEHHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCEEEE
RIESGAVRIGEKVTIYPGGRESAIGTIRTFPSSEAASASYGEAIGFTLEAADAEIVRGSV
EECCCCEEECCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEECCEE
IAAGAPPTCGREFQATVFWFMDEYAAGDPVTIRCTTQSVPGRILLHQVFDPAQPDAELPS
EECCCCCCCCCCCEEEEEEEEHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCC
DLICVGEAARCTILTEVDLAGDHPGLIPETGRFVIEREGIPAGAGIFR
CEEEECCCCEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Adenylylsulfate

Specific reaction: ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]