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The map label for this gene is paaJ [H]

Identifier: 197117821

GI number: 197117821

Start: 1656494

End: 1657690

Strand: Direct

Name: paaJ [H]

Synonym: Gbem_1434

Alternate gene names: 197117821

Gene position: 1656494-1657690 (Clockwise)

Preceding gene: 197117820

Following gene: 197117822

Centisome position: 35.89

GC content: 66.83

Gene sequence:

>1197_bases
ATGCGAGAAGCGGTTATCGTCGATGCGGTGCGGACGCCGGTGGGGAAATTCGGAGGCGCGCTGAAGGACGTCCGCCCGGA
CGACCTGGCGGCACTTTGCATCATGGAGCTGGTGCAGCGGAACAAGCTCGACCCCGGGCTCATCGAGGACGTGGTGCTTG
GCTGCACCAATCAGGCAGGAGAGGACAACCGCAACGTGGCGCGCATGGCGGCGCTCCTGGCGGGGCTCCCGCACTCGGTG
GCGGGGCACACCATCAACCGCCTCTGCGGTTCGGGGTTGAACGCCATCAACAGCGCCGCCCAGGCCATCAAGGTGGGGGA
GGGGAAGATCTTCATCGCCGGGGGGACCGAGTCCATGACCCGCGCACCCTTCGTCTTCGCCAAGGCCGATTCCCCCTTCT
CGCGCGACATCAAGGTGTTCGATTCCACCATCGGGTGGCGCTTCACCAACCCCCGGATGACCGAACCGTATGCCAAGGAA
GGAATGGGGGACACCGCCGAGAACGTGGCGCGCAGCTACGGCATCACCCGCGAGCAGCAGGACGAGTTCGCGCTGGCGAC
CCAGAGGAAATGGGGCGAGGCCCAGGCCGCGGGGAAGTTCGAGGACGAGCTGGTTCCGGTCGTCATCCCGCAGAAGAAGG
GGGACCCGAAGGTCGTGGACCGGGACGAGTTCCCGCGCCCGGACGTGACGCTGGAGCAACTCGCCAAACTTTCCCCCGCC
TTCAAGAAGGACGGCAGCGTCACCGCGGGTAATTCCAGCGGCATCAACGACGGCGCCGCCGCCGTGCTCCTCATGGAAGG
GGAGCTTGCCCGAGAGCTCGGCTACCGGCCGCTGGCGCGCATTCTCTCCAGCGCCGTCGCCGGCTGCGACCCCTCGTTCA
TGGGGCTCGGCCCGGTTCCGGCCATAAGGAAGGCTCTGGAAAGGGCGGGGCTGACCATCGGCGATATCGACCTCTTCGAG
CTGAACGAGGCCTTCGCGGCGCAGGCCATACCTTGCATGAGCGAGCTGGGGATAGACCCGGCCAAGGTGAACGTGAACGG
CGGATCCATTGCCATCGGCCATCCTCTCGGCTCCACCGGCGCCCGCATCACCGCGACGCTCGTGCACGAGATGCGGCGGC
GTAACGCCCGCTACGGCGTGATCTCGCTCTGCATAGGCCTTGGGCAGGGGATCGCCACCGTGGTGGAACGGGTCTGA

Upstream 100 bases:

>100_bases
GAAAATTGCCGCCCCTTCGCAAAAGGGGACAGGCTACTTTTCAAAATAAAAAAGTAGCCTGTCCCCCTTTCGGGGGCGCG
GCCGAGATATGGAGGTTGCA

Downstream 100 bases:

>100_bases
AACGGATGTAAGGGGGAGAAACCATGCCGGCAACCGAAGAGAAACCCCTCAGGATCGATTTCCACGTGCACCTCGCCACG
TACGACGGTTTCCTGCACCC

Product: thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MREAVIVDAVRTPVGKFGGALKDVRPDDLAALCIMELVQRNKLDPGLIEDVVLGCTNQAGEDNRNVARMAALLAGLPHSV
AGHTINRLCGSGLNAINSAAQAIKVGEGKIFIAGGTESMTRAPFVFAKADSPFSRDIKVFDSTIGWRFTNPRMTEPYAKE
GMGDTAENVARSYGITREQQDEFALATQRKWGEAQAAGKFEDELVPVVIPQKKGDPKVVDRDEFPRPDVTLEQLAKLSPA
FKKDGSVTAGNSSGINDGAAAVLLMEGELARELGYRPLARILSSAVAGCDPSFMGLGPVPAIRKALERAGLTIGDIDLFE
LNEAFAAQAIPCMSELGIDPAKVNVNGGSIAIGHPLGSTGARITATLVHEMRRRNARYGVISLCIGLGQGIATVVERV

Sequences:

>Translated_398_residues
MREAVIVDAVRTPVGKFGGALKDVRPDDLAALCIMELVQRNKLDPGLIEDVVLGCTNQAGEDNRNVARMAALLAGLPHSV
AGHTINRLCGSGLNAINSAAQAIKVGEGKIFIAGGTESMTRAPFVFAKADSPFSRDIKVFDSTIGWRFTNPRMTEPYAKE
GMGDTAENVARSYGITREQQDEFALATQRKWGEAQAAGKFEDELVPVVIPQKKGDPKVVDRDEFPRPDVTLEQLAKLSPA
FKKDGSVTAGNSSGINDGAAAVLLMEGELARELGYRPLARILSSAVAGCDPSFMGLGPVPAIRKALERAGLTIGDIDLFE
LNEAFAAQAIPCMSELGIDPAKVNVNGGSIAIGHPLGSTGARITATLVHEMRRRNARYGVISLCIGLGQGIATVVERV
>Mature_398_residues
MREAVIVDAVRTPVGKFGGALKDVRPDDLAALCIMELVQRNKLDPGLIEDVVLGCTNQAGEDNRNVARMAALLAGLPHSV
AGHTINRLCGSGLNAINSAAQAIKVGEGKIFIAGGTESMTRAPFVFAKADSPFSRDIKVFDSTIGWRFTNPRMTEPYAKE
GMGDTAENVARSYGITREQQDEFALATQRKWGEAQAAGKFEDELVPVVIPQKKGDPKVVDRDEFPRPDVTLEQLAKLSPA
FKKDGSVTAGNSSGINDGAAAVLLMEGELARELGYRPLARILSSAVAGCDPSFMGLGPVPAIRKALERAGLTIGDIDLFE
LNEAFAAQAIPCMSELGIDPAKVNVNGGSIAIGHPLGSTGARITATLVHEMRRRNARYGVISLCIGLGQGIATVVERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=44.9748743718593, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI148539872, Length=395, Percent_Identity=42.7848101265823, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI4501853, Length=403, Percent_Identity=43.6724565756824, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI4557237, Length=399, Percent_Identity=36.0902255639098, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI4504327, Length=428, Percent_Identity=33.8785046728972, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI194353979, Length=396, Percent_Identity=31.5656565656566, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=57.1072319201995, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI87082165, Length=406, Percent_Identity=46.7980295566502, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1788554, Length=401, Percent_Identity=49.1271820448878, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI48994986, Length=408, Percent_Identity=46.5686274509804, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=34.7931873479319, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=45.2261306532663, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=38, Blast_Score=255, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=33.5839598997494, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17551802, Length=429, Percent_Identity=33.1002331002331, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17535917, Length=401, Percent_Identity=30.6733167082294, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322031, Length=401, Percent_Identity=41.8952618453865, Blast_Score=260, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=36.2282878411911, Blast_Score=232, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24655093, Length=398, Percent_Identity=43.4673366834171, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=41.8546365914787, Blast_Score=297, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24640423, Length=402, Percent_Identity=36.5671641791045, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=34.7417840375587, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42204; Mature: 42204

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREAVIVDAVRTPVGKFGGALKDVRPDDLAALCIMELVQRNKLDPGLIEDVVLGCTNQAG
CCCCEEHHHHHCCHHHHCCHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
EDNRNVARMAALLAGLPHSVAGHTINRLCGSGLNAINSAAQAIKVGEGKIFIAGGTESMT
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCEEEEECCCCCHH
RAPFVFAKADSPFSRDIKVFDSTIGWRFTNPRMTEPYAKEGMGDTAENVARSYGITREQQ
CCCEEEEECCCCCCCCHHHHHCCCCEEECCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCC
DEFALATQRKWGEAQAAGKFEDELVPVVIPQKKGDPKVVDRDEFPRPDVTLEQLAKLSPA
HHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCH
FKKDGSVTAGNSSGINDGAAAVLLMEGELARELGYRPLARILSSAVAGCDPSFMGLGPVP
HHCCCCEECCCCCCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHH
AIRKALERAGLTIGDIDLFELNEAFAAQAIPCMSELGIDPAKVNVNGGSIAIGHPLGSTG
HHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCC
ARITATLVHEMRRRNARYGVISLCIGLGQGIATVVERV
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MREAVIVDAVRTPVGKFGGALKDVRPDDLAALCIMELVQRNKLDPGLIEDVVLGCTNQAG
CCCCEEHHHHHCCHHHHCCHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
EDNRNVARMAALLAGLPHSVAGHTINRLCGSGLNAINSAAQAIKVGEGKIFIAGGTESMT
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCEEEEECCCCCHH
RAPFVFAKADSPFSRDIKVFDSTIGWRFTNPRMTEPYAKEGMGDTAENVARSYGITREQQ
CCCEEEEECCCCCCCCHHHHHCCCCEEECCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCC
DEFALATQRKWGEAQAAGKFEDELVPVVIPQKKGDPKVVDRDEFPRPDVTLEQLAKLSPA
HHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCH
FKKDGSVTAGNSSGINDGAAAVLLMEGELARELGYRPLARILSSAVAGCDPSFMGLGPVP
HHCCCCEECCCCCCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHH
AIRKALERAGLTIGDIDLFELNEAFAAQAIPCMSELGIDPAKVNVNGGSIAIGHPLGSTG
HHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCC
ARITATLVHEMRRRNARYGVISLCIGLGQGIATVVERV
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]