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The map label for this gene is cspLA [H]

Identifier: 197117779

GI number: 197117779

Start: 1605963

End: 1606163

Strand: Direct

Name: cspLA [H]

Synonym: Gbem_1391

Alternate gene names: 197117779

Gene position: 1605963-1606163 (Clockwise)

Preceding gene: 197117774

Following gene: 197117780

Centisome position: 34.8

GC content: 51.74

Gene sequence:

>201_bases
ATGGCAAACGGAACTGTAAAATGGTTTAACGACAGCAAGGGTTTTGGTTTTCTCGAGCAGGAAAACGGCGACGATGTTTT
CTGCCACTTCTCCGCTATCACTGGCGAAGGATTCAAATCCCTTGTTGAAGGCGACAGCGTCACCTTCGACGTAGTCAGGG
GGCCGAAAGGTCTTCAGGCTGCCAACGTGACCAGGGTCTAG

Upstream 100 bases:

>100_bases
CGATATTTAAATAGCTTGCTATGTAGATTCGAAGCTGTTACGATGAGATTCAATCTGGAACAACTCCGGGTAAATTCAGA
AATTTTGGAGATATAAAGTA

Downstream 100 bases:

>100_bases
ATTCAGCCACCTGGCAGGACCATTGGTTCCTGCACCGTCATACGGCTCACTAACGGACGAGGCCCCTGTCAATAAAACAG
GGGCCTCGTCTTTTGTAGCG

Product: cold shock DNA/RNA-binding protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 65

Protein sequence:

>66_residues
MANGTVKWFNDSKGFGFLEQENGDDVFCHFSAITGEGFKSLVEGDSVTFDVVRGPKGLQAANVTRV

Sequences:

>Translated_66_residues
MANGTVKWFNDSKGFGFLEQENGDDVFCHFSAITGEGFKSLVEGDSVTFDVVRGPKGLQAANVTRV
>Mature_65_residues
ANGTVKWFNDSKGFGFLEQENGDDVFCHFSAITGEGFKSLVEGDSVTFDVVRGPKGLQAANVTRV

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=60.655737704918, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=57.8125, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=50.7462686567164, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=54.6875, Blast_Score=69, Evalue=8e-14,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=54.0983606557377, Blast_Score=65, Evalue=7e-13,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=50, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=46.875, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7129; Mature: 6998

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGTVKWFNDSKGFGFLEQENGDDVFCHFSAITGEGFKSLVEGDSVTFDVVRGPKGLQA
CCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCHHHHHCCCCEEEEEEECCCCCCC
ANVTRV
CCEECC
>Mature Secondary Structure 
ANGTVKWFNDSKGFGFLEQENGDDVFCHFSAITGEGFKSLVEGDSVTFDVVRGPKGLQA
CCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCHHHHHCCCCEEEEEEECCCCCCC
ANVTRV
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]