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The map label for this gene is hyaS [H]

Identifier: 197117757

GI number: 197117757

Start: 1576785

End: 1577960

Strand: Reverse

Name: hyaS [H]

Synonym: Gbem_1369

Alternate gene names: 197117757

Gene position: 1577960-1576785 (Counterclockwise)

Preceding gene: 197117761

Following gene: 197117756

Centisome position: 34.19

GC content: 69.22

Gene sequence:

>1176_bases
ATGAAAGATCTTCAGCAGTGGGCAGAGGGCGACACCTTCGGGGATTTGCTGAAACGGCGGGGGGTAACCAGGCGTGAATT
CCTCTCCTTTTGCGGCAAGATGGCGGCGCTGATCGGCACCGGCGGGGTCTTCGCGGGAAGCCGGACCGCATGGGCGCAGG
AACTCGCCGGGCGGCTGGAAGGGGCGCGGCGCCCGAGCGTGGTCTACCTGCAGTTGCAGGAGTGCACCGGCTGTCTGGAG
AGCCTGCTCCGTTCCGCGAGCACCCCGGTCGAGGAACTGGTCCTGGAGCAGATCTCGCTCGACTACAACGAGCTCCTGAT
GGCTCCCTCGGGAGAGGCGGCCGAACAGGCGCTCGCCGCAGCACAAGGAAAGCCGCACCTCCTCTTGGTGAACGGCTCGG
TGCCGCTCAAAAACGGCGGGGTCTACTGCACCATCGGCGGCCGCTCCGCGCGCGACGTGCTCGAGCGCGCAGCGGCCAAC
GCCACCGCGGTCGTTGCCATAGGCGCCTGCGCCGAGTACGGCTGCGTCCAGGCCGCCGCCCCCAACCCCACCGGCGCCGT
GGGGGTAGCCGACGTGATCAGGGACCGCCCGGTGGTGAACGTAAGCGGCTGCCCCCCCATCGCCGAGACCATCAGCGCCA
CCCTCACCTACTACCTCGCCTACGGCCGCACGCCGCCTCTGGACGGGCTGGGACGCCCGCTCTTCGCCTACGGCCAGCGC
ATACACGACAAGTGCCCCCGGCGCGCAAGTTTCGACGCCGGGCAGTTCGCGGAGCGCTTCGACGACCAGAACGCCCGCCT
GGGGCACTGCCTCTACCGGCTGGGGTGCAAGGGGCCGGCCACCTTCGCCCCCTGCGCCACCATCGAATGGAACGACGGGT
TGAGCTTCCCGATCAAGGCGGGGCATCCCTGCCTCGGCTGCACCGAGCGCCACTTCTACGACCGGATGACCCCATTCTAC
CGGCGCCTCCCCGGCATCGTGGTCCCGGGGCTCGGGGTGGAAGCGACCGCCAACACCATAGGCGTTGCGGCCGTCGCCGC
TTCGGTCGCCGCGGTCGCGGTCCACTCCGCAGCGACGGTGATAGCGAAGCACCGGGCGCGCCGGGCCGAGCCGGAAAGCC
TGCCGCTGGCGGTACTGGGAGACAGGAAGGAAGCTGACGAGAAGGAAAAGGATTAG

Upstream 100 bases:

>100_bases
CCGTAATATCGAGCCGGAAATGTTCTGCCTCGGAAGGACTCCCTCGGCAGAATCGTCTCCGTTTTGTGGCACCGGTCCAG
GGCCGGATGGAGCATGGGAC

Downstream 100 bases:

>100_bases
GCCTTCCCGAGCGGCAAGGAGAAACCAGATGGCAACAGAGCGCATCGTCATTGACCCGATCACCAGGATCGAGGGGCACC
TGAGAATAGAGCTTGAGGCG

Product: [NiFe]-hydrogenase small subunit

Products: NA

Alternate protein names: HYD1; Membrane-bound hydrogenase 1 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKDLQQWAEGDTFGDLLKRRGVTRREFLSFCGKMAALIGTGGVFAGSRTAWAQELAGRLEGARRPSVVYLQLQECTGCLE
SLLRSASTPVEELVLEQISLDYNELLMAPSGEAAEQALAAAQGKPHLLLVNGSVPLKNGGVYCTIGGRSARDVLERAAAN
ATAVVAIGACAEYGCVQAAAPNPTGAVGVADVIRDRPVVNVSGCPPIAETISATLTYYLAYGRTPPLDGLGRPLFAYGQR
IHDKCPRRASFDAGQFAERFDDQNARLGHCLYRLGCKGPATFAPCATIEWNDGLSFPIKAGHPCLGCTERHFYDRMTPFY
RRLPGIVVPGLGVEATANTIGVAAVAASVAAVAVHSAATVIAKHRARRAEPESLPLAVLGDRKEADEKEKD

Sequences:

>Translated_391_residues
MKDLQQWAEGDTFGDLLKRRGVTRREFLSFCGKMAALIGTGGVFAGSRTAWAQELAGRLEGARRPSVVYLQLQECTGCLE
SLLRSASTPVEELVLEQISLDYNELLMAPSGEAAEQALAAAQGKPHLLLVNGSVPLKNGGVYCTIGGRSARDVLERAAAN
ATAVVAIGACAEYGCVQAAAPNPTGAVGVADVIRDRPVVNVSGCPPIAETISATLTYYLAYGRTPPLDGLGRPLFAYGQR
IHDKCPRRASFDAGQFAERFDDQNARLGHCLYRLGCKGPATFAPCATIEWNDGLSFPIKAGHPCLGCTERHFYDRMTPFY
RRLPGIVVPGLGVEATANTIGVAAVAASVAAVAVHSAATVIAKHRARRAEPESLPLAVLGDRKEADEKEKD
>Mature_391_residues
MKDLQQWAEGDTFGDLLKRRGVTRREFLSFCGKMAALIGTGGVFAGSRTAWAQELAGRLEGARRPSVVYLQLQECTGCLE
SLLRSASTPVEELVLEQISLDYNELLMAPSGEAAEQALAAAQGKPHLLLVNGSVPLKNGGVYCTIGGRSARDVLERAAAN
ATAVVAIGACAEYGCVQAAAPNPTGAVGVADVIRDRPVVNVSGCPPIAETISATLTYYLAYGRTPPLDGLGRPLFAYGQR
IHDKCPRRASFDAGQFAERFDDQNARLGHCLYRLGCKGPATFAPCATIEWNDGLSFPIKAGHPCLGCTERHFYDRMTPFY
RRLPGIVVPGLGVEATANTIGVAAVAASVAAVAVHSAATVIAKHRARRAEPESLPLAVLGDRKEADEKEKD

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=365, Percent_Identity=45.4794520547945, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1789371, Length=300, Percent_Identity=43.6666666666667, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 41537; Mature: 41537

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLQQWAEGDTFGDLLKRRGVTRREFLSFCGKMAALIGTGGVFAGSRTAWAQELAGRLE
CCCHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GARRPSVVYLQLQECTGCLESLLRSASTPVEELVLEQISLDYNELLMAPSGEAAEQALAA
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHEEECCCCHHHHHHHHH
AQGKPHLLLVNGSVPLKNGGVYCTIGGRSARDVLERAAANATAVVAIGACAEYGCVQAAA
HCCCCEEEEEECCCEECCCCEEEEECCCHHHHHHHHHHCCCEEEEEEHHHHHHCCEECCC
PNPTGAVGVADVIRDRPVVNVSGCPPIAETISATLTYYLAYGRTPPLDGLGRPLFAYGQR
CCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
IHDKCPRRASFDAGQFAERFDDQNARLGHCLYRLGCKGPATFAPCATIEWNDGLSFPIKA
HHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
GHPCLGCTERHFYDRMTPFYRRLPGIVVPGLGVEATANTIGVAAVAASVAAVAVHSAATV
CCCCCCCCHHHHHHHCCHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IAKHRARRAEPESLPLAVLGDRKEADEKEKD
HHHHHHHCCCCCCCCEEEECCCHHCCCCCCC
>Mature Secondary Structure
MKDLQQWAEGDTFGDLLKRRGVTRREFLSFCGKMAALIGTGGVFAGSRTAWAQELAGRLE
CCCHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GARRPSVVYLQLQECTGCLESLLRSASTPVEELVLEQISLDYNELLMAPSGEAAEQALAA
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHEEECCCCHHHHHHHHH
AQGKPHLLLVNGSVPLKNGGVYCTIGGRSARDVLERAAANATAVVAIGACAEYGCVQAAA
HCCCCEEEEEECCCEECCCCEEEEECCCHHHHHHHHHHCCCEEEEEEHHHHHHCCEECCC
PNPTGAVGVADVIRDRPVVNVSGCPPIAETISATLTYYLAYGRTPPLDGLGRPLFAYGQR
CCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
IHDKCPRRASFDAGQFAERFDDQNARLGHCLYRLGCKGPATFAPCATIEWNDGLSFPIKA
HHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
GHPCLGCTERHFYDRMTPFYRRLPGIVVPGLGVEATANTIGVAAVAASVAAVAVHSAATV
CCCCCCCCHHHHHHHCCHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IAKHRARRAEPESLPLAVLGDRKEADEKEKD
HHHHHHHCCCCCCCCEEEECCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA