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The map label for this gene is dhaT [H]

Identifier: 197117741

GI number: 197117741

Start: 1559749

End: 1560912

Strand: Reverse

Name: dhaT [H]

Synonym: Gbem_1353

Alternate gene names: 197117741

Gene position: 1560912-1559749 (Counterclockwise)

Preceding gene: 197117749

Following gene: 197117740

Centisome position: 33.82

GC content: 61.25

Gene sequence:

>1164_bases
ATGGCATTAGGAGAGCAGACGTACGGTTTCTACATTCCGACAGTATCACTGATGGGTATTGGTTCCGCTAAAGAGACCGG
TGGCCAGATCAAGGCACTGGGCGCATCCAAGGCGCTGATCGTTACCGACAAAGGCCTCTCGGCCATGGGCGTTGCCGACA
AGATCAAATCCCAGGTTGAAGAGGCCGGTGTTTCCGCAGTCATCTTCGACGGCGCAGAGCCCAACCCGACCGACATCAAC
GTGCACGACGGCGTGAAGGTGTACCAGGACAACGGCTGTGACGCGATCATCTCCCTGGGCGGCGGTTCCTCCCATGACTG
CGCTAAAGGCATCGGCATGGTCATCGGCAACGGCGGCCACATCCGCGATCTCGAAGGCGTGAACAAGACCACCAAGCCGA
TGCCGGCATTCGTGGCCATCAACACCACCGCAGGCACCGCGTCCGAAATGACCCGTTTCTGCATCATCACCAACACCGAC
ACCCACGTGAAGATGGCGATCGTCGACTGGCGCTGCACCCCGAACGTCGCGATCAACGACCCGCTGCTCATGGTCGGCAA
GCCGGCGGCACTGACCGCGGCAACCGGCATGGACGCACTGACCCACGCCGTCGAGGCGTACGTGTCCACCATCGCTACCC
CGATCACCGACGCTTGCGCCATCAAGGCAATCGAGCTGATCGCCGAGTTCCTCTCCAAGGCAGTTGCCAACGGCGAAGAC
CTCGAGGCGCGCGACAAGATGGCTTACGCCGAGTACCTGGCCGGCATGGCGTTCAACAACGCATCGCTTGGCTACGTTCA
CTCCATGGCTCACCAGCTGGGCGGCTTCTACAACCTGCCGCACGGCGTCTGCAACGCCATCCTGCTCCCGGCCGTCAGCC
AGTACAACCTGATCGCTTGCCCGAAGCGTTTCGCCGACATCGCGAAAGCCCTCGGCGAGAACATCGACGGCCTCTCCGTG
ACCGAAGCAGGCCAGAAGGCAATCGACAGGATCCGCACCCTCTCCGCTTCCATCGGCATCCCGACCGGCCTCAAGGCCCT
CAACGTCAAGGAAGCCGACCTCACCATCATGGCTGAGAACGCGAAGAAGGACGCTTGCCAGTTCACCAACCCGCGCAAGG
CAACCCTTGAGCAGGTCGTCCAGATCTTCAAGGACGCAATGTAA

Upstream 100 bases:

>100_bases
ACCTAGAAAACCGTTTTAGGACAGTGTATGCAACTTGGACCTACGCAATGGTTAAACAACCGGGCTTGAAATCCGGACAC
AACAACAGGGGGAAAGACAA

Downstream 100 bases:

>100_bases
GAACCAGCAGCATAGCAGCAAAGCTCCCCTCCCCCTGGCGGGGGAGGGGTTTTGAATCTCCGAGCCATGCGCCGCCTAAA
AGGGACCACCCTCACGGAGC

Product: ethanol dehydrogenase

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MALGEQTYGFYIPTVSLMGIGSAKETGGQIKALGASKALIVTDKGLSAMGVADKIKSQVEEAGVSAVIFDGAEPNPTDIN
VHDGVKVYQDNGCDAIISLGGGSSHDCAKGIGMVIGNGGHIRDLEGVNKTTKPMPAFVAINTTAGTASEMTRFCIITNTD
THVKMAIVDWRCTPNVAINDPLLMVGKPAALTAATGMDALTHAVEAYVSTIATPITDACAIKAIELIAEFLSKAVANGED
LEARDKMAYAEYLAGMAFNNASLGYVHSMAHQLGGFYNLPHGVCNAILLPAVSQYNLIACPKRFADIAKALGENIDGLSV
TEAGQKAIDRIRTLSASIGIPTGLKALNVKEADLTIMAENAKKDACQFTNPRKATLEQVVQIFKDAM

Sequences:

>Translated_387_residues
MALGEQTYGFYIPTVSLMGIGSAKETGGQIKALGASKALIVTDKGLSAMGVADKIKSQVEEAGVSAVIFDGAEPNPTDIN
VHDGVKVYQDNGCDAIISLGGGSSHDCAKGIGMVIGNGGHIRDLEGVNKTTKPMPAFVAINTTAGTASEMTRFCIITNTD
THVKMAIVDWRCTPNVAINDPLLMVGKPAALTAATGMDALTHAVEAYVSTIATPITDACAIKAIELIAEFLSKAVANGED
LEARDKMAYAEYLAGMAFNNASLGYVHSMAHQLGGFYNLPHGVCNAILLPAVSQYNLIACPKRFADIAKALGENIDGLSV
TEAGQKAIDRIRTLSASIGIPTGLKALNVKEADLTIMAENAKKDACQFTNPRKATLEQVVQIFKDAM
>Mature_386_residues
ALGEQTYGFYIPTVSLMGIGSAKETGGQIKALGASKALIVTDKGLSAMGVADKIKSQVEEAGVSAVIFDGAEPNPTDINV
HDGVKVYQDNGCDAIISLGGGSSHDCAKGIGMVIGNGGHIRDLEGVNKTTKPMPAFVAINTTAGTASEMTRFCIITNTDT
HVKMAIVDWRCTPNVAINDPLLMVGKPAALTAATGMDALTHAVEAYVSTIATPITDACAIKAIELIAEFLSKAVANGEDL
EARDKMAYAEYLAGMAFNNASLGYVHSMAHQLGGFYNLPHGVCNAILLPAVSQYNLIACPKRFADIAKALGENIDGLSVT
EAGQKAIDRIRTLSASIGIPTGLKALNVKEADLTIMAENAKKDACQFTNPRKATLEQVVQIFKDAM

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=380, Percent_Identity=27.3684210526316, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI48994951, Length=378, Percent_Identity=56.0846560846561, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789163, Length=374, Percent_Identity=43.3155080213904, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI87082107, Length=382, Percent_Identity=35.3403141361257, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1787493, Length=383, Percent_Identity=34.9869451697128, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1789386, Length=348, Percent_Identity=25.5747126436782, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=424, Percent_Identity=24.2924528301887, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321181, Length=381, Percent_Identity=52.49343832021, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24657991, Length=425, Percent_Identity=25.8823529411765, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 40522; Mature: 40391

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGEQTYGFYIPTVSLMGIGSAKETGGQIKALGASKALIVTDKGLSAMGVADKIKSQVE
CCCCCCCCEEEECCEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
EAGVSAVIFDGAEPNPTDINVHDGVKVYQDNGCDAIISLGGGSSHDCAKGIGMVIGNGGH
HCCCEEEEECCCCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCHHHHCCCEEEECCCC
IRDLEGVNKTTKPMPAFVAINTTAGTASEMTRFCIITNTDTHVKMAIVDWRCTPNVAIND
EECCCCCCCCCCCCCEEEEEECCCCCHHHCEEEEEEECCCCEEEEEEEEEECCCCCEECC
PLLMVGKPAALTAATGMDALTHAVEAYVSTIATPITDACAIKAIELIAEFLSKAVANGED
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEARDKMAYAEYLAGMAFNNASLGYVHSMAHQLGGFYNLPHGVCNAILLPAVSQYNLIAC
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEC
PKRFADIAKALGENIDGLSVTEAGQKAIDRIRTLSASIGIPTGLKALNVKEADLTIMAEN
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECC
AKKDACQFTNPRKATLEQVVQIFKDAM
CCCHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALGEQTYGFYIPTVSLMGIGSAKETGGQIKALGASKALIVTDKGLSAMGVADKIKSQVE
CCCCCCCEEEECCEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
EAGVSAVIFDGAEPNPTDINVHDGVKVYQDNGCDAIISLGGGSSHDCAKGIGMVIGNGGH
HCCCEEEEECCCCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCHHHHCCCEEEECCCC
IRDLEGVNKTTKPMPAFVAINTTAGTASEMTRFCIITNTDTHVKMAIVDWRCTPNVAIND
EECCCCCCCCCCCCCEEEEEECCCCCHHHCEEEEEEECCCCEEEEEEEEEECCCCCEECC
PLLMVGKPAALTAATGMDALTHAVEAYVSTIATPITDACAIKAIELIAEFLSKAVANGED
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEARDKMAYAEYLAGMAFNNASLGYVHSMAHQLGGFYNLPHGVCNAILLPAVSQYNLIAC
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEC
PKRFADIAKALGENIDGLSVTEAGQKAIDRIRTLSASIGIPTGLKALNVKEADLTIMAEN
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECC
AKKDACQFTNPRKATLEQVVQIFKDAM
CCCHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]