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The map label for this gene is dut

Identifier: 197117698

GI number: 197117698

Start: 1514323

End: 1514763

Strand: Direct

Name: dut

Synonym: Gbem_1310

Alternate gene names: 197117698

Gene position: 1514323-1514763 (Clockwise)

Preceding gene: 197117697

Following gene: 197117699

Centisome position: 32.81

GC content: 65.76

Gene sequence:

>441_bases
ATGGGTAGCCTGCCTGTCCGGATAAAGAGGTTGAGAGCAACCCCGCTGCCGGCCTACATGACCGAGCACGCGGCCGGCGT
CGACCTCTGCGCGTCGCTCTCCGCCGATTTCGTGCTGGCGCCTGGCGAGAGGGCACTGGTACCCACCGGGCTTGCCATAG
AACTCCCGCCGGGGTTCGAGGCGCAGGTGAGGCCGAGAAGCGGGCTCGCTTTACGCCACGGCATCGCGCTTGTTAACTCG
CCGGGAACCATTGACGCCGACTACCGCGGCGAGATCGGGGTGATCCTGATCAACCTGGGGAGCGAGCCGTTCACGGTGAG
CGACGGCGAGCGTATCGCCCAGATGGTTTTTGCCCGCTGTGAGCGTGCCGAGTTCATTGAGGTCGATGAGTTAGGCGACA
CCGCGCGCGGCGCCGGCGGCTTCGGGCATACGGGTAGATAG

Upstream 100 bases:

>100_bases
ACCCTCGCCGTAGATATCCTCGACAATTCCGCGCTGACCCTGGTCATGCTGGGGCGGATCGGTACGCCTTCTTTCAAAGC
TTCGGACATAAACGTCTGAG

Downstream 100 bases:

>100_bases
AGAAAGAAGCAGATAGAGAAAGAAGCAGATAAAGATAGAGATAAAGATAAAGATTGAGACTAAGACTAATCTAAGACTAA
GACTCTGGGCCTCATATTGC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MGSLPVRIKRLRATPLPAYMTEHAAGVDLCASLSADFVLAPGERALVPTGLAIELPPGFEAQVRPRSGLALRHGIALVNS
PGTIDADYRGEIGVILINLGSEPFTVSDGERIAQMVFARCERAEFIEVDELGDTARGAGGFGHTGR

Sequences:

>Translated_146_residues
MGSLPVRIKRLRATPLPAYMTEHAAGVDLCASLSADFVLAPGERALVPTGLAIELPPGFEAQVRPRSGLALRHGIALVNS
PGTIDADYRGEIGVILINLGSEPFTVSDGERIAQMVFARCERAEFIEVDELGDTARGAGGFGHTGR
>Mature_145_residues
GSLPVRIKRLRATPLPAYMTEHAAGVDLCASLSADFVLAPGERALVPTGLAIELPPGFEAQVRPRSGLALRHGIALVNSP
GTIDADYRGEIGVILINLGSEPFTVSDGERIAQMVFARCERAEFIEVDELGDTARGAGGFGHTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI4503423, Length=147, Percent_Identity=43.5374149659864, Blast_Score=110, Evalue=5e-25,
Organism=Homo sapiens, GI70906444, Length=144, Percent_Identity=43.75, Blast_Score=108, Evalue=1e-24,
Organism=Homo sapiens, GI70906441, Length=147, Percent_Identity=43.5374149659864, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1790071, Length=136, Percent_Identity=49.2647058823529, Blast_Score=127, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71988561, Length=145, Percent_Identity=41.3793103448276, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319729, Length=144, Percent_Identity=35.4166666666667, Blast_Score=92, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583610, Length=141, Percent_Identity=39.7163120567376, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19921126, Length=141, Percent_Identity=39.7163120567376, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_GEOBB (B5EI65)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138125.1
- GeneID:   6781297
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1310
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- ProtClustDB:   PRK00601
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15410; Mature: 15279

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: BINDING 79-79

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLPVRIKRLRATPLPAYMTEHAAGVDLCASLSADFVLAPGERALVPTGLAIELPPGFE
CCCCCCEEEEEECCCCCHHHHHCCCCHHHHHHCCCCEEECCCCCEEECCCCEEECCCCCC
AQVRPRSGLALRHGIALVNSPGTIDADYRGEIGVILINLGSEPFTVSDGERIAQMVFARC
EEECCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEEEECCCCEEECCHHHHHHHHHHHC
ERAEFIEVDELGDTARGAGGFGHTGR
CCCCEEEHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GSLPVRIKRLRATPLPAYMTEHAAGVDLCASLSADFVLAPGERALVPTGLAIELPPGFE
CCCCCEEEEEECCCCCHHHHHCCCCHHHHHHCCCCEEECCCCCEEECCCCEEECCCCCC
AQVRPRSGLALRHGIALVNSPGTIDADYRGEIGVILINLGSEPFTVSDGERIAQMVFARC
EEECCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEEEECCCCEEECCHHHHHHHHHHHC
ERAEFIEVDELGDTARGAGGFGHTGR
CCCCEEEHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA