Click here to switch to the map view.

The map label for this gene is pnp

Identifier: 197117696

GI number: 197117696

Start: 1510921

End: 1513011

Strand: Direct

Name: pnp

Synonym: Gbem_1308

Alternate gene names: 197117696

Gene position: 1510921-1513011 (Clockwise)

Preceding gene: 197117695

Following gene: 197117697

Centisome position: 32.74

GC content: 63.27

Gene sequence:

>2091_bases
ATGGTACACACCGTACAGGCAGAATGCTGTGGCAAGACAATCACCATCGAAACGGGAAAGATCGCGAAGCAGGCCAGCGG
CGCAGTCATGATCAAAAGCGGCGACACCATGGTGCTCGTCACCGCCGTCGCCATGAAGAGCGCCAAGGAAGGGCAGGGGT
TCTTCCCGCTCACCGTCAACTACCAGGAGAAGGCGTACGCGGGCGGCCGCATCCCCGGGTCCTTCTTCAAGCGCGAGGGG
CGTCCCTCCGACAACGAGACCCTGACCTGCCGTCTCATCGACCGCCCGATCCGTCCGCTGTTCCCGGAGAACTTCCTTAA
CGACACCCAGATCATGGCGACCGTGGTTTCGGCCGACAAGGACAACGACCCCGGCATCCTCTCCATGATCGGCGCCTCCG
CGGCCCTGATGGTTTCCGACGTCCCCTTCGCAGGCCCCATCGCCGGCGTCAAGGTCGGCCGCGTCGACGGCCAGTTCATC
GCCAACCCGACTGCGGAGCAGGAAGAAAAGAGCGACCTCGAGATCGTGATCGCCGCCAGCCAGGACGCAATCCTGATGGT
GGAAGGTTCCGCCTGCGAGGTTTCCGAGGACGACCTGCTGGAAGCCATTTTCTTCGGCCACAAAGCGGTGCAGCCGGTCC
TCGCCGCCCAGCTCGAGCTCGCCAAGAAAGTCGGCACCTCGAAGCGCGAAATCCCGGCGCCGGTGGTGAACGAGGCGCTG
AAGGCGCGCGTATCCGCTCTCGCCAAAGAGGGGATGAAGCAGGCGGTCCGCATCAAGACCAAGGTGGAGCGCCACCTGGC
CATCGACGCCATCGCGGACGAGACCGTCGCGGCACTTTCGGCCGAGTTCGAAGGCTCCGAGAAAGAAATCAAAGGTTTCA
TCGAAGACCTCGAGTACGACCTCGTGCGTGAGCACATCATCAAGGACGGCCAGAGGATCGACGGGCGCGATACCAAGACC
ATCCGCGCCATCTCCACCGAAGTAAGCCTGCTCCCCCGCGCCCACGGCTCCGCCCTGTTCACCCGCGGCGAGACCCAGTC
CATCGTGGCGGCGACGCTCGGCACCTCGGTCGACGAGCAGCGCATCGACTCGCTCTACGGCGACTCCAGGAAGAAGTTCA
TGCTGCACTACAACTTCCCTCCGTACTCCGTCGGCGAGACCAGCTTCCGCCTTGCCCCGGGGCGCCGCGAGATCGGCCAC
GGCATGCTCGCCGAGCGCGCCCTGCAGCAGGTGCTCCCGAAGCATGACGACTTCCCCTACACCATCAGGATCGTTTCCGA
CATCACCGAGAGCAACGGCTCCTCTTCGATGGCGACCGTCTGCGGCGGCTCGCTGTCCATGATGGACGCAGGCATCCCGA
TCAAGGCGCCGGTTGCCGGCATCGCCATGGGCCTCATCAAGGAAGGCGACGATTTCGCCATCCTCTCCGACATCCTCGGC
GACGAAGACCACCTGGGCGACATGGACTTCAAAGTGGCCGGTACCGCCGAAGGCGTCACCGCGCTGCAGATGGACATCAA
GATCGGCGGCGTCACCCGCGAGATCATGAGCGCGGCGCTGGCTCAGGCCAAGGCAGGCAGGATCCACATCCTGGGCGAGA
TGGCCAAGACCATCGGGGCATCGCGCGGCGACCTCTCCGCCTTCGCGCCTCGCATCACCACCATCTGGGTCAAAGTCGAC
AAGATCCGCGACGTCATCGGGAGCGGCGGCAAGAACATCAGGAGCGTCACCGAGGCGACCGGCGTCTCCATCGACATCGA
CGACACCGGCAAGATCAACATCGCTTCCACCAACAAGGAAGCCTGCGACCTCGCCATCAAGATGATCAGGAACCTCACCG
CCGAGGCCGAGGAAGGGAAGCTCTACATGGGCACCGTCAAAAAGATCATGGAGTTCGGCGCCTTCGTGGAGATCTTCCCG
GGAACCGACGGCCTGGTCCACGTCTCCGAGCTCGACACCGAGCGCGTCAAGAACGTGAGCGACATCCTCAAAGAGGGCGA
CAAGGTCCTGGTCAAGTGCATCGGCATCGACAAGCAGGGCAAGATCAAGCTGTCCCGTAAAGAGGCTCTGGGACTGACTT
TCACCGAATAA

Upstream 100 bases:

>100_bases
GCGTGGATAAAACCGCCTTGGGAGCTTGTCCGGCTCGCAGGGCGCTTTTATCGGCGGCCCCAACACCAACCGTAAAAAAG
TTTAAATCGGAGGCCGAAGA

Downstream 100 bases:

>100_bases
TCCTTCTCATTTGAAAGCTGGCAATTTATGGCAATCCAGGTATAGTTCTAGCCATGATTAAAAAGACCATTCTAAATAAC
GGGATACGTGTCATCACCGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MVHTVQAECCGKTITIETGKIAKQASGAVMIKSGDTMVLVTAVAMKSAKEGQGFFPLTVNYQEKAYAGGRIPGSFFKREG
RPSDNETLTCRLIDRPIRPLFPENFLNDTQIMATVVSADKDNDPGILSMIGASAALMVSDVPFAGPIAGVKVGRVDGQFI
ANPTAEQEEKSDLEIVIAASQDAILMVEGSACEVSEDDLLEAIFFGHKAVQPVLAAQLELAKKVGTSKREIPAPVVNEAL
KARVSALAKEGMKQAVRIKTKVERHLAIDAIADETVAALSAEFEGSEKEIKGFIEDLEYDLVREHIIKDGQRIDGRDTKT
IRAISTEVSLLPRAHGSALFTRGETQSIVAATLGTSVDEQRIDSLYGDSRKKFMLHYNFPPYSVGETSFRLAPGRREIGH
GMLAERALQQVLPKHDDFPYTIRIVSDITESNGSSSMATVCGGSLSMMDAGIPIKAPVAGIAMGLIKEGDDFAILSDILG
DEDHLGDMDFKVAGTAEGVTALQMDIKIGGVTREIMSAALAQAKAGRIHILGEMAKTIGASRGDLSAFAPRITTIWVKVD
KIRDVIGSGGKNIRSVTEATGVSIDIDDTGKINIASTNKEACDLAIKMIRNLTAEAEEGKLYMGTVKKIMEFGAFVEIFP
GTDGLVHVSELDTERVKNVSDILKEGDKVLVKCIGIDKQGKIKLSRKEALGLTFTE

Sequences:

>Translated_696_residues
MVHTVQAECCGKTITIETGKIAKQASGAVMIKSGDTMVLVTAVAMKSAKEGQGFFPLTVNYQEKAYAGGRIPGSFFKREG
RPSDNETLTCRLIDRPIRPLFPENFLNDTQIMATVVSADKDNDPGILSMIGASAALMVSDVPFAGPIAGVKVGRVDGQFI
ANPTAEQEEKSDLEIVIAASQDAILMVEGSACEVSEDDLLEAIFFGHKAVQPVLAAQLELAKKVGTSKREIPAPVVNEAL
KARVSALAKEGMKQAVRIKTKVERHLAIDAIADETVAALSAEFEGSEKEIKGFIEDLEYDLVREHIIKDGQRIDGRDTKT
IRAISTEVSLLPRAHGSALFTRGETQSIVAATLGTSVDEQRIDSLYGDSRKKFMLHYNFPPYSVGETSFRLAPGRREIGH
GMLAERALQQVLPKHDDFPYTIRIVSDITESNGSSSMATVCGGSLSMMDAGIPIKAPVAGIAMGLIKEGDDFAILSDILG
DEDHLGDMDFKVAGTAEGVTALQMDIKIGGVTREIMSAALAQAKAGRIHILGEMAKTIGASRGDLSAFAPRITTIWVKVD
KIRDVIGSGGKNIRSVTEATGVSIDIDDTGKINIASTNKEACDLAIKMIRNLTAEAEEGKLYMGTVKKIMEFGAFVEIFP
GTDGLVHVSELDTERVKNVSDILKEGDKVLVKCIGIDKQGKIKLSRKEALGLTFTE
>Mature_696_residues
MVHTVQAECCGKTITIETGKIAKQASGAVMIKSGDTMVLVTAVAMKSAKEGQGFFPLTVNYQEKAYAGGRIPGSFFKREG
RPSDNETLTCRLIDRPIRPLFPENFLNDTQIMATVVSADKDNDPGILSMIGASAALMVSDVPFAGPIAGVKVGRVDGQFI
ANPTAEQEEKSDLEIVIAASQDAILMVEGSACEVSEDDLLEAIFFGHKAVQPVLAAQLELAKKVGTSKREIPAPVVNEAL
KARVSALAKEGMKQAVRIKTKVERHLAIDAIADETVAALSAEFEGSEKEIKGFIEDLEYDLVREHIIKDGQRIDGRDTKT
IRAISTEVSLLPRAHGSALFTRGETQSIVAATLGTSVDEQRIDSLYGDSRKKFMLHYNFPPYSVGETSFRLAPGRREIGH
GMLAERALQQVLPKHDDFPYTIRIVSDITESNGSSSMATVCGGSLSMMDAGIPIKAPVAGIAMGLIKEGDDFAILSDILG
DEDHLGDMDFKVAGTAEGVTALQMDIKIGGVTREIMSAALAQAKAGRIHILGEMAKTIGASRGDLSAFAPRITTIWVKVD
KIRDVIGSGGKNIRSVTEATGVSIDIDDTGKINIASTNKEACDLAIKMIRNLTAEAEEGKLYMGTVKKIMEFGAFVEIFP
GTDGLVHVSELDTERVKNVSDILKEGDKVLVKCIGIDKQGKIKLSRKEALGLTFTE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=709, Percent_Identity=37.7997179125529, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI145693187, Length=678, Percent_Identity=55.3097345132743, Blast_Score=762, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=701, Percent_Identity=34.9500713266762, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI281362905, Length=704, Percent_Identity=37.0738636363636, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=704, Percent_Identity=37.0738636363636, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=704, Percent_Identity=37.0738636363636, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=36.1832061068702, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_GEOBB (B5EI63)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138123.1
- ProteinModelPortal:   B5EI63
- GeneID:   6781295
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1308
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 74702; Mature: 74702

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHTVQAECCGKTITIETGKIAKQASGAVMIKSGDTMVLVTAVAMKSAKEGQGFFPLTVN
CCEEEEHHHCCCEEEEECCCHHHHCCCEEEEECCCCEEEEEHHHHHCCCCCCCEEEEEEC
YQEKAYAGGRIPGSFFKREGRPSDNETLTCRLIDRPIRPLFPENFLNDTQIMATVVSADK
CCHHHCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCHHHHHEEECCCC
DNDPGILSMIGASAALMVSDVPFAGPIAGVKVGRVDGQFIANPTAEQEEKSDLEIVIAAS
CCCCCHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCEEEEEEEC
QDAILMVEGSACEVSEDDLLEAIFFGHKAVQPVLAAQLELAKKVGTSKREIPAPVVNEAL
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
KARVSALAKEGMKQAVRIKTKVERHLAIDAIADETVAALSAEFEGSEKEIKGFIEDLEYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LVREHIIKDGQRIDGRDTKTIRAISTEVSLLPRAHGSALFTRGETQSIVAATLGTSVDEQ
HHHHHHHCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCHH
RIDSLYGDSRKKFMLHYNFPPYSVGETSFRLAPGRREIGHGMLAERALQQVLPKHDDFPY
HHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCHHHHHCHHHHHHHHHHHCCCCCCCCE
TIRIVSDITESNGSSSMATVCGGSLSMMDAGIPIKAPVAGIAMGLIKEGDDFAILSDILG
EEEEEEHHHCCCCCCCCHHHCCCCCEEHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCC
DEDHLGDMDFKVAGTAEGVTALQMDIKIGGVTREIMSAALAQAKAGRIHILGEMAKTIGA
CCCCCCCCCEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHCC
SRGDLSAFAPRITTIWVKVDKIRDVIGSGGKNIRSVTEATGVSIDIDDTGKINIASTNKE
CCCCHHHHCCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCEEEEECCCHH
ACDLAIKMIRNLTAEAEEGKLYMGTVKKIMEFGAFVEIFPGTDGLVHVSELDTERVKNVS
HHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCEEEEECCCCCEEEHHHCCHHHHHHHH
DILKEGDKVLVKCIGIDKQGKIKLSRKEALGLTFTE
HHHHCCCEEEEEEECCCCCCCEEEEHHHHCCCEECC
>Mature Secondary Structure
MVHTVQAECCGKTITIETGKIAKQASGAVMIKSGDTMVLVTAVAMKSAKEGQGFFPLTVN
CCEEEEHHHCCCEEEEECCCHHHHCCCEEEEECCCCEEEEEHHHHHCCCCCCCEEEEEEC
YQEKAYAGGRIPGSFFKREGRPSDNETLTCRLIDRPIRPLFPENFLNDTQIMATVVSADK
CCHHHCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCHHHHHEEECCCC
DNDPGILSMIGASAALMVSDVPFAGPIAGVKVGRVDGQFIANPTAEQEEKSDLEIVIAAS
CCCCCHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCEEEEEEEC
QDAILMVEGSACEVSEDDLLEAIFFGHKAVQPVLAAQLELAKKVGTSKREIPAPVVNEAL
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
KARVSALAKEGMKQAVRIKTKVERHLAIDAIADETVAALSAEFEGSEKEIKGFIEDLEYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LVREHIIKDGQRIDGRDTKTIRAISTEVSLLPRAHGSALFTRGETQSIVAATLGTSVDEQ
HHHHHHHCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCHH
RIDSLYGDSRKKFMLHYNFPPYSVGETSFRLAPGRREIGHGMLAERALQQVLPKHDDFPY
HHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCHHHHHCHHHHHHHHHHHCCCCCCCCE
TIRIVSDITESNGSSSMATVCGGSLSMMDAGIPIKAPVAGIAMGLIKEGDDFAILSDILG
EEEEEEHHHCCCCCCCCHHHCCCCCEEHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCC
DEDHLGDMDFKVAGTAEGVTALQMDIKIGGVTREIMSAALAQAKAGRIHILGEMAKTIGA
CCCCCCCCCEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHCC
SRGDLSAFAPRITTIWVKVDKIRDVIGSGGKNIRSVTEATGVSIDIDDTGKINIASTNKE
CCCCHHHHCCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCEEEEECCCHH
ACDLAIKMIRNLTAEAEEGKLYMGTVKKIMEFGAFVEIFPGTDGLVHVSELDTERVKNVS
HHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCEEEEECCCCCEEEHHHCCHHHHHHHH
DILKEGDKVLVKCIGIDKQGKIKLSRKEALGLTFTE
HHHHCCCEEEEEEECCCCCCCEEEEHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA