Click here to switch to the map view.

The map label for this gene is thrC [H]

Identifier: 197117686

GI number: 197117686

Start: 1499588

End: 1500964

Strand: Reverse

Name: thrC [H]

Synonym: Gbem_1298

Alternate gene names: 197117686

Gene position: 1500964-1499588 (Counterclockwise)

Preceding gene: 197117711

Following gene: 197117685

Centisome position: 32.52

GC content: 62.53

Gene sequence:

>1377_bases
ATGAAATACATAAGCACCAGGGGAAAGATAGCGCCGGTCGGTTTCAAGGACGCGGTCATGATGGGACTTGCCACCGACGG
CGGGCTGATTCTTCCGGAATCAATCCCGCAGCTCGACCGCGGCACCTTGGCCGCCTGGTCCAAGCTCCCCTACCGCGAGC
TTGCCTTCAACGTCATCTCCCTTTTCGCCACCGACATACCGGCTTTCGACCTGAAGGCGCTCATCGACCGTTCCTACGGC
AGCTTCGAGCACGAGGAGACCACGCCGCTGGTGCAAAAGGACGGCGTCTACATATTGGAGCTCTTCCACGGCCCCACCCT
CGCCTTCAAGGACGTGGCGCTGCAGCTTCTTGGGAACCTCTTCGAATACCTTTTGAAGGAGCGCGGGGAGAAGATGAACA
TCCTCGGCGCCACCTCGGGCGACACCGGGAGCGCGGCCATTGCCGGGGTGCGCGGCAAGGAGAACATCAACATCTTCATC
CTGCATCCGCACCTGAAGACCTCGCCGATCCAGGCGCTGCAGATGACCAGCGTACTCGACGAGAACGTGCACAACGTCGC
GGTGGAGGGGACCTTCGACGACTGCCAGAACATCGTGAAGGCCCTCTTCAACGACCTGCAGTTCAAGAAGGAGTACGCGC
TCGGCGCGGTGAACTCGATCAACTGGGCGCGCGTTTTGGCCCAGGTGGTCTACTACTTCTACGCCTGGGGTAGGCTCCCG
GAGCAAAAAGAGGTGATCTTCTCCGTCCCCACCGGCAACTTCGGCGACATCTTCGCCGGCTACCTCGCGAAAAGGATGGG
GCTCCCGGTGGAGAAGCTGCTTTTGGCCACCAACGAGAACAACATCCTCGCCCGCTTCGTGCAAACCGGCGACTATTCGC
TCGGGAAGGTGGTGCAGACCGTCTCCCCCTCCATGGACATCCAGCTCGCCTCCAACCTTGAGCGCTACCTCTACTACCTC
TGGGACGAGAACCCGGAGAAGGTCTGCAGCGCCTTCGCCGAATTGCAGTCGACCGGCAGGATCGTGTTCACCAAGGAGCA
GGTGCTAAGGGTGCAGGCGGAGTTCATGAGCTGCACGGTGGACGAGCAGAAGACCCTGGATACCATCGCCTCCTTCAACC
GCGAAACCGGCTACCTCCTCGACCCACACACCGCGGTCGGCGTGCGCGGCGCCCTGGAATGCGCGCCGGGTCACCCGAGG
GTGGTCTGCCTCGCCACGGCACACCCGGCGAAATTCGGGGATGCCGTCGAGCGCGCCGTAGGCTCACCTCCCCCGCTTCC
CCCGGCACTCGCGGCCCTGGTGGGTAAAGAGACCCGCTGCGAGCTGATGGCGCCCGACCGGGAGAAGATCAAGGCGTTCG
TCAAGGCCAAGGCGTAA

Upstream 100 bases:

>100_bases
ATACTGCCCGGCCAGCAATCGCTTCACTTTTAAACTTTTAACTGCTATATATTGACGTTGCCGAAACGGCAAAACCACCT
ACAGCAGAAAGAGAATCCAC

Downstream 100 bases:

>100_bases
AGAGCAGCATCAGGCGGATCGGCTCCTGCCGATCCGCCTGCCGAGGAAAACCCTACATGCCCCTCCCTCTGAAAATCGAC
TGGACCCTCATCGACACCGT

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKYISTRGKIAPVGFKDAVMMGLATDGGLILPESIPQLDRGTLAAWSKLPYRELAFNVISLFATDIPAFDLKALIDRSYG
SFEHEETTPLVQKDGVYILELFHGPTLAFKDVALQLLGNLFEYLLKERGEKMNILGATSGDTGSAAIAGVRGKENINIFI
LHPHLKTSPIQALQMTSVLDENVHNVAVEGTFDDCQNIVKALFNDLQFKKEYALGAVNSINWARVLAQVVYYFYAWGRLP
EQKEVIFSVPTGNFGDIFAGYLAKRMGLPVEKLLLATNENNILARFVQTGDYSLGKVVQTVSPSMDIQLASNLERYLYYL
WDENPEKVCSAFAELQSTGRIVFTKEQVLRVQAEFMSCTVDEQKTLDTIASFNRETGYLLDPHTAVGVRGALECAPGHPR
VVCLATAHPAKFGDAVERAVGSPPPLPPALAALVGKETRCELMAPDREKIKAFVKAKA

Sequences:

>Translated_458_residues
MKYISTRGKIAPVGFKDAVMMGLATDGGLILPESIPQLDRGTLAAWSKLPYRELAFNVISLFATDIPAFDLKALIDRSYG
SFEHEETTPLVQKDGVYILELFHGPTLAFKDVALQLLGNLFEYLLKERGEKMNILGATSGDTGSAAIAGVRGKENINIFI
LHPHLKTSPIQALQMTSVLDENVHNVAVEGTFDDCQNIVKALFNDLQFKKEYALGAVNSINWARVLAQVVYYFYAWGRLP
EQKEVIFSVPTGNFGDIFAGYLAKRMGLPVEKLLLATNENNILARFVQTGDYSLGKVVQTVSPSMDIQLASNLERYLYYL
WDENPEKVCSAFAELQSTGRIVFTKEQVLRVQAEFMSCTVDEQKTLDTIASFNRETGYLLDPHTAVGVRGALECAPGHPR
VVCLATAHPAKFGDAVERAVGSPPPLPPALAALVGKETRCELMAPDREKIKAFVKAKA
>Mature_458_residues
MKYISTRGKIAPVGFKDAVMMGLATDGGLILPESIPQLDRGTLAAWSKLPYRELAFNVISLFATDIPAFDLKALIDRSYG
SFEHEETTPLVQKDGVYILELFHGPTLAFKDVALQLLGNLFEYLLKERGEKMNILGATSGDTGSAAIAGVRGKENINIFI
LHPHLKTSPIQALQMTSVLDENVHNVAVEGTFDDCQNIVKALFNDLQFKKEYALGAVNSINWARVLAQVVYYFYAWGRLP
EQKEVIFSVPTGNFGDIFAGYLAKRMGLPVEKLLLATNENNILARFVQTGDYSLGKVVQTVSPSMDIQLASNLERYLYYL
WDENPEKVCSAFAELQSTGRIVFTKEQVLRVQAEFMSCTVDEQKTLDTIASFNRETGYLLDPHTAVGVRGALECAPGHPR
VVCLATAHPAKFGDAVERAVGSPPPLPPALAALVGKETRCELMAPDREKIKAFVKAKA

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI149193323, Length=457, Percent_Identity=39.3873085339169, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI153792148, Length=506, Percent_Identity=35.3754940711462, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1786185, Length=434, Percent_Identity=33.8709677419355, Blast_Score=218, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6319901, Length=457, Percent_Identity=48.7964989059081, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 50397; Mature: 50397

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYISTRGKIAPVGFKDAVMMGLATDGGLILPESIPQLDRGTLAAWSKLPYRELAFNVIS
CCCCCCCCCCCCCCHHHHHHEEEECCCCEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHH
LFATDIPAFDLKALIDRSYGSFEHEETTPLVQKDGVYILELFHGPTLAFKDVALQLLGNL
HHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCEEEEEEECCCCHHHHHHHHHHHHHH
FEYLLKERGEKMNILGATSGDTGSAAIAGVRGKENINIFILHPHLKTSPIQALQMTSVLD
HHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
ENVHNVAVEGTFDDCQNIVKALFNDLQFKKEYALGAVNSINWARVLAQVVYYFYAWGRLP
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
EQKEVIFSVPTGNFGDIFAGYLAKRMGLPVEKLLLATNENNILARFVQTGDYSLGKVVQT
CCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCEEEEEECCCCCCHHHHHHH
VSPSMDIQLASNLERYLYYLWDENPEKVCSAFAELQSTGRIVFTKEQVLRVQAEFMSCTV
CCCCCCCHHHHCHHHEEEEEECCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCC
DEQKTLDTIASFNRETGYLLDPHTAVGVRGALECAPGHPRVVCLATAHPAKFGDAVERAV
CHHHHHHHHHHCCCCCCCEECCCHHHCCCCCEECCCCCCEEEEEEECCCHHHHHHHHHHC
GSPPPLPPALAALVGKETRCELMAPDREKIKAFVKAKA
CCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure
MKYISTRGKIAPVGFKDAVMMGLATDGGLILPESIPQLDRGTLAAWSKLPYRELAFNVIS
CCCCCCCCCCCCCCHHHHHHEEEECCCCEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHH
LFATDIPAFDLKALIDRSYGSFEHEETTPLVQKDGVYILELFHGPTLAFKDVALQLLGNL
HHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCEEEEEEECCCCHHHHHHHHHHHHHH
FEYLLKERGEKMNILGATSGDTGSAAIAGVRGKENINIFILHPHLKTSPIQALQMTSVLD
HHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
ENVHNVAVEGTFDDCQNIVKALFNDLQFKKEYALGAVNSINWARVLAQVVYYFYAWGRLP
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
EQKEVIFSVPTGNFGDIFAGYLAKRMGLPVEKLLLATNENNILARFVQTGDYSLGKVVQT
CCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCEEEEEECCCCCCHHHHHHH
VSPSMDIQLASNLERYLYYLWDENPEKVCSAFAELQSTGRIVFTKEQVLRVQAEFMSCTV
CCCCCCCHHHHCHHHEEEEEECCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCC
DEQKTLDTIASFNRETGYLLDPHTAVGVRGALECAPGHPRVVCLATAHPAKFGDAVERAV
CHHHHHHHHHHCCCCCCCEECCCHHHCCCCCEECCCCCCEEEEEEECCCHHHHHHHHHHC
GSPPPLPPALAALVGKETRCELMAPDREKIKAFVKAKA
CCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1333566; 10984043 [H]