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The map label for this gene is rph [H]

Identifier: 197117592

GI number: 197117592

Start: 1398492

End: 1399211

Strand: Direct

Name: rph [H]

Synonym: Gbem_1204

Alternate gene names: 197117592

Gene position: 1398492-1399211 (Clockwise)

Preceding gene: 197117591

Following gene: 197117593

Centisome position: 30.3

GC content: 64.72

Gene sequence:

>720_bases
ATGATACGCAGAGAAGGCCGCGCGGCCAACGCCTTGAGAGAAGTTAAGATCACCCGCAATTACCTGAAGCACGCCGAGGG
CTCTGTCCTGGTCGAGTTTGGGGACACCAAAGTCATCTGCAACGCAACGGTCGAGGCGAGGGTACCTTCGTTTCTGAAAG
GGAAGGGGAGCGGCTGGGTCACGGCGGAGTACTGCATGCTGCCGCGCGCCACGCACACCAGGAACCAGCGCGAATCTGCC
AAGGGGAAACTCTCCGGCCGCACCCACGAGATCCAGAGGCTGATCGGCAGGTCGCTGCGCGCGGTGGTCGACCTGGAGCT
TCTGGGCGAGCGCTCCATCGTGATCGACTGCGACGTGATCCAGGCCGACGGCGGCACCCGCACCGCCTCCATCACCGGCG
CCTACGTGGCCTTGGCGGACGCGCTGCAGAACCTGGTCAACGCCGGCGAACTCGAGAAGAGCCCCCTCAGGGAGGCGGTC
GCCGCCGTAAGCGTGGGGATCGTCGACGGCGTTCCCTTCCTCGACCTCGACTACCCCGAGGACTCCACCGCCGAAGTCGA
CATGAACTTCGTGATGACCTCGTCCATGCGCTTTGTCGAGGTGCAGGGGACTGCCGAGGCGGAGCCTTTCACCCTCGACC
AGATGGACGCCATGCGCAGGCACGCGATGCTCGGCCTCTCCCGCCTCTTCGAGATCCAGCAGGAGGCTTTGCAGGCATGA

Upstream 100 bases:

>100_bases
GGAGCGGGGAAGGTCATATTCACCCCGCGGCTGACAGCACCGCCTGAAGCAGGTTCCAAAGAAAAACCCTAAACTTTTTA
CCCAGAGAGGATGGAGATAC

Downstream 100 bases:

>100_bases
AAGAGCTCCTGGTAGCGTCGGGGAACAAGGGGAAGCTGCGCGAATTCGAGAAGCTTTTGGAGGGGGTGGTGGAGACCATC
CTCTCCCCTGCCGACTTCCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIRREGRAANALREVKITRNYLKHAEGSVLVEFGDTKVICNATVEARVPSFLKGKGSGWVTAEYCMLPRATHTRNQRESA
KGKLSGRTHEIQRLIGRSLRAVVDLELLGERSIVIDCDVIQADGGTRTASITGAYVALADALQNLVNAGELEKSPLREAV
AAVSVGIVDGVPFLDLDYPEDSTAEVDMNFVMTSSMRFVEVQGTAEAEPFTLDQMDAMRRHAMLGLSRLFEIQQEALQA

Sequences:

>Translated_239_residues
MIRREGRAANALREVKITRNYLKHAEGSVLVEFGDTKVICNATVEARVPSFLKGKGSGWVTAEYCMLPRATHTRNQRESA
KGKLSGRTHEIQRLIGRSLRAVVDLELLGERSIVIDCDVIQADGGTRTASITGAYVALADALQNLVNAGELEKSPLREAV
AAVSVGIVDGVPFLDLDYPEDSTAEVDMNFVMTSSMRFVEVQGTAEAEPFTLDQMDAMRRHAMLGLSRLFEIQQEALQA
>Mature_239_residues
MIRREGRAANALREVKITRNYLKHAEGSVLVEFGDTKVICNATVEARVPSFLKGKGSGWVTAEYCMLPRATHTRNQRESA
KGKLSGRTHEIQRLIGRSLRAVVDLELLGERSIVIDCDVIQADGGTRTASITGAYVALADALQNLVNAGELEKSPLREAV
AAVSVGIVDGVPFLDLDYPEDSTAEVDMNFVMTSSMRFVEVQGTAEAEPFTLDQMDAMRRHAMLGLSRLFEIQQEALQA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=68.0952380952381, Blast_Score=294, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71981632, Length=186, Percent_Identity=27.4193548387097, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26166; Mature: 26166

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRREGRAANALREVKITRNYLKHAEGSVLVEFGDTKVICNATVEARVPSFLKGKGSGWV
CCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHCCCHHHCCCCCCCE
TAEYCMLPRATHTRNQRESAKGKLSGRTHEIQRLIGRSLRAVVDLELLGERSIVIDCDVI
EEHHEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
QADGGTRTASITGAYVALADALQNLVNAGELEKSPLREAVAAVSVGIVDGVPFLDLDYPE
ECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCC
DSTAEVDMNFVMTSSMRFVEVQGTAEAEPFTLDQMDAMRRHAMLGLSRLFEIQQEALQA
CCCCEEEEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRREGRAANALREVKITRNYLKHAEGSVLVEFGDTKVICNATVEARVPSFLKGKGSGWV
CCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHCCCHHHCCCCCCCE
TAEYCMLPRATHTRNQRESAKGKLSGRTHEIQRLIGRSLRAVVDLELLGERSIVIDCDVI
EEHHEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
QADGGTRTASITGAYVALADALQNLVNAGELEKSPLREAVAAVSVGIVDGVPFLDLDYPE
ECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCC
DSTAEVDMNFVMTSSMRFVEVQGTAEAEPFTLDQMDAMRRHAMLGLSRLFEIQQEALQA
CCCCEEEEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA